US Patent No. 9,933,430

METHOD AND KIT FOR DISTINGUISHING BETWEEN PROSTATE CARCINOMA AND BENIGN PROSTATIC HYPERPLASIA


Patent No. 9,933,430
Issue Date April 03, 2018
Title Method And Kit For Distinguishing Between Prostate Carcinoma And Benign Prostatic Hyperplasia
Inventorship Chikara Ohyama, Hirosaki (JP)
Tohru Yoneyama, Hirosaki (JP)
Yuki Tobisawa, Hirosaki (JP)
Shingo Hatakeyama, Hirosaki (JP)
Takashi Suzuki, Shizuoka (JP)
Ilpal Jwa, Shizuoka (JP)
Maho Yamaguchi, Shizuoka (JP)
Assignee HIROSAKI UNIVERSITY, Hirosaki-shi (JP) SHIZUOKA PREFECTURAL UNIVERSITY COR...

Claim of US Patent No. 9,933,430

1. A method for analyzing an analyte sample, comprising:bringing an analyte sample containing a prostate-specific antigen (PSA) into contact with a carrier having an anti-free PSA antibody immobilized thereon, thereby binding free PSA to the anti-free PSA antibody immobilized on the carrier;
thereafter bringing the carrier in which the free PSA is bound to the immobilized anti-free PSA antibody into contact with a monoclonal antibody capable of specifically recognizing galactose having a terminal sialic acid residue through an ?(2,3) bond in an N-type glycan, thereby binding the monoclonal antibody to the free PSA bound to the anti-free PSA antibody immobilized on the carrier; and
measuring the amount of the monoclonal antibody bound to free PSA, thereby measuring the amount of free PSA having an N-type glycan in which a terminal sialic acid residue is bound to galactose through an ?(2,3) bond.