US Patent No. 9,933,363

SYSTEM AND METHOD FOR DENSE-STOCHASTIC-SAMPLING IMAGING


Patent No. 9,933,363
Issue Date April 03, 2018
Title System And Method For Dense-stochastic-sampling Imaging
Inventorship Gaudenz Danuser, Cambridge, MA (US)
Paul C. Goodwin, Shoreline, WA (US)
Assignee GE HEALTHCARE BIO-SCIENCES CORP., Piscataway, NJ (US)

Claim of US Patent No. 9,933,363

21. An apparatus comprising:a position-sensitive detector configured for detecting radiation as a function of position on the detector;an optical system characterized by a diffraction-limited resolution volume and configured for imaging radiation emitted from optical labels in a sample onto the position sensitive-detector, wherein the optical labels are distributed in the sample with a density greater than an inverse of the diffraction-limited resolution volume of the optical system;a radiation source configured to provide radiation to the sample to induce emission of fluorescence radiation from a plurality of subsets of the optical labels in the sample, wherein different subsets of the optical labels emit fluorescence radiation at different times;a controller configured to control the radiation provided to the sample such that a mean volume per fluorescing optical label in the subsets is less than the diffraction-limited resolution volume of the optical system;a controller configured to control the position-sensitive detector to record images of different subsets of the optical labels at different times.