US Patent No. 9,826,743

COMPOSITIONS COMPRISING RECOMBINANT BACILLUS CELLS AND ANOTHER BIOLOGICAL CONTROL AGENT


Patent No. 9,826,743
Issue Date November 28, 2017
Title Compositions Comprising Recombinant Bacillus Cells And Another Biological Control Agent
Inventorship Damian Curtis, Davis, CA (US)
Brian Thompson, Creve Coeur, MO (US)
Assignee Bayer CropScience LP, Research Triangle Park, NC (US)

Claim of US Patent No. 9,826,743

1. A composition comprising: a) recombinant Bacillus cereus family member cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide
selected from the group consisting of an endoglucanase with at least 85% sequence identity to SEQ ID NO: 107 and a phospholipase
with at least 85% sequence identity to SEQ ID NO: 108; and (ii) a targeting sequence, exosporium protein, or exosporium protein
fragment comprising an amino acid sequence having at least 43% identity with amino acids 20-35 of SEQ ID NO: 1, wherein the
identity with amino acids 25-35 is at least 54%: a targeting sequence comprising amino acids 1-35 of SEQ ID NO: 1: a targeting
sequence comprising amino acids 20-35 of SEQ ID NO: 1: a targeting sequence comprising amino acids 22-31 of SEQ ID NO: 1:
a targeting sequence comprising amino acids 22-33 of SEQ ID NO: 1: a targeting sequence comprising amino acids 20-31 of SEQ
ID NO: 1: a targeting sequence comprising SEQ ID NO: 1: or an exosporium protein comprising an amino acid sequence having
at least 85% identity with SEQ ID NO: 2; and b) at least one biological control agent selected from the group consisting of
Bacillus subtilis QST713, Bacillus subtilis MBI600, Bacillus amyloliquefaciens D747, Bacillus amyloliquefaciens FZB42, Bacillus subtilis var. amyloliquefaciens FZB24, and Bacillus subtilis Y1336 in a synergistically effective amount, wherein the synergy is between a) and b).