US Patent No. 9,783,584

METHODS AND COMPOSITIONS FOR EXPRESSING FUNCTIONAL CLASS XIV MYOSIN


Patent No. 9,783,584
Issue Date October 10, 2017
Title Methods And Compositions For Expressing Functional Class Xiv Myosin
Inventorship Kathleen M. Trybus, Burlington, VT (US)
Gary E. Ward, Burlington, VT (US)
Assignee University of Vermont and State Agricultural College, Burlington, VT (US)

Claim of US Patent No. 9,783,584

1. A method of producing a functional class XIV myosin polypeptide, the method comprising:
co-expressing three or more polynucleotides in a heterologous expression-system cell, wherein the three or more polynucleotides
comprise a class XIV heavy chain polypeptide-encoding polynucleotide, a myosin light chain polypeptide-encoding polynucleotide,
and a parasite co-chaperone polypeptide-encoding polynucleotide, wherein the three or more polynucleotides are co-expressed
in the cell under conditions suitable to produce a functional class XIV myosin polypeptide comprising the class XIV heavy
chain polypeptide and the myosin light chain polypeptide, and wherein the encoded parasite co-chaperone polypeptide comprises
an amino acid sequence set forth as SEQ ID NO: 4 or a functional variant thereof that has an amino acid sequence with at least
75% sequence similarity to SEQ ID NO: 4 or SEQ ID NO: 12 or a functional fragment thereof that has an amino acid sequence
with at least 75% sequence similarity to SEQ ID NO: 12.