US Patent No. 9,746,420

DIFFERENTIAL SCAN IMAGING SYSTEMS AND METHODS


Patent No. 9,746,420
Issue Date August 29, 2017
Title Differential Scan Imaging Systems And Methods
Inventorship Ahmed Bouzid, Lincoln, NE (US)
Assignee Li-Cor, Inc., Lincoln, NE (US)

Claim of US Patent No. 9,746,420

1. An imaging system, comprising:
a sample platform; and
an illumination and detection system, including:
an illumination system that provides a beam of excitation light from a source to a target area on the sample platform;
a sensor adapted to detect light and having an array of sensing locations; and
one or more optical elements arranged and configured to image light emanating from the sample platform onto the sensor, wherein
fluorescent light from a portion of the target area illuminated by the excitation light is imaged onto only a first portion
of the sensor comprising a first plurality of the sensing locations and background light from proximal to the portion of the
target area illuminated by the excitation light on the platform is simultaneously imaged onto only a second portion of the
sensor comprising a second plurality of the sensing locations different from the first plurality of the sensing locations;
and

a scanning mechanism configured to adjust a relative position of the sample platform and the illumination and detection system
such that the portion of the target area illuminated by the excitation beam changes at each of a plurality of scan positions,
and

wherein, at each scan position, a second signal detected by the second portion of the sensor is subtracted from a first signal
simultaneously detected by the first portion of the sensor to produce a background-reduced signal for the scan position of
the plurality of scan positions.