US Patent No. 9,598,684

ROTHIA SPECIES GLUTEN-DEGRADING ENZYMES AND USES THEREOF


Patent No. 9,598,684
Issue Date March 21, 2017
Title Rothia Species Gluten-degrading Enzymes And Uses Thereof
Inventorship Eva J. Helmerhorst, Chestnut Hill, MA (US)
Frank G. Oppenheim, Chestnut Hill, MA (US)
Assignee TRUSTEES OF BOSTON UNIVERSITY, Boston, MA (US)

Claim of US Patent No. 9,598,684

1. A lyophilized probiotic composition comprising an effective amount of Rothia species bacteria and a pharmaceutically acceptable carrier, wherein the bacteria comprises a gluten-degrading enzyme that
retains protease activity at acidic pH of 3.0 as measured in an in vitro gliadin degradation assay for 3 hours using a synthetic
substrate Z-YPQ-pNA, wherein the enzyme, when purified, comprises an iso-electric point within a pH range of 2.0-7.0 inclusive,
and wherein the composition is formulated into granules.