US Patent No. 9,408,901

COMPOSITIONS, METHODS AND USES FOR DENGUE VIRUS SEROTYPE-4 CONSTRUCTS


Patent No. 9,408,901
Issue Date August 09, 2016
Title Compositions, Methods And Uses For Dengue Virus Serotype-4 Constructs
Inventorship Jill A. Livengood, Fort Collins, CO (US)
Claire Y. Huang, Fort Collins, CO (US)
Timothy D. Powell, Fort Collins, CO (US)
Dan T. Stinchcomb, Fort Collins, CO (US)
Jorge Osorio, Mount Horeb, WI (US)
Assignee TAKEDA VACCINES, INC, Deerfield, IL (US)

Claim of US Patent No. 9,408,901

1. A nucleic acid molecule comprising a chimeric flavivirus construct comprising nucleic acid sequences encoding nonstructural
proteins and one or more structural proteins from a live, attenuated dengue-2 virus and at least encoding one or more structural
proteins from a second dengue virus, the second flavivirus is selected from the group dengue-1, dengue-3 or dengue-4 virus
wherein the chimeric construct further comprises one or more mutations comprising, a mutation in an envelope (E) protein at
an amino acid position synonymous of dengue 4 to amino acid 417 when the E protein is from the second flavivirus wherein the
mutation in the envelope (E) protein at the position synonymous to amino acid 417 changes glutamic acid to a positively charged
residue; a mutation in the capsid protein at an amino acid position synonymous to amino acid 107 in the attenuated dengue-2
wherein the mutation in the capsid (C) protein at the position synonymous to amino acid 107 changes cysteine to an aromatic
amino acid residue in the attenuated dengue-2, and a mutation in NS4A at an amino acid position synonymous to amino acid position
17 in the attenuated dengue-2.