US Patent No. 9,335,533

ADJUSTABLE TOTAL INTERNAL REFLECTANCE MICROSCOPY (TIRFM) ILLUMINATOR APPARATUS


Patent No. 9,335,533
Issue Date May 10, 2016
Title Adjustable Total Internal Reflectance Microscopy (tirfm) Illuminator Apparatus
Inventorship Guy Kennedy, Underhill Center, VT (US)
David M. Warshaw, South Burlington, VT (US)
Assignee The University of Vermont and State Agricultural College, Burlington, VT (...

Claim of US Patent No. 9,335,533

1. An adjustable total internal reflectance microscopy (TIRFM) illuminator apparatus, the apparatus comprising:
an objective lens wherein the objective lens comprises:
an optical axis,
an outer circumference;
inner circumference
at least one optical fiber, wherein the at least one optical fiber is adaptable to transmit at least one optical excitation
light wavelength through the outer circumference of the objective lens;

at least one fiber axial translator for focusing the at least one optical excitation light wavelength, wherein the at least
one fiber axial translator is mechanically adjustable in at least one-degree-of freedom;

at least one collimating optical element connectable to the at least optical fiber for impinging the at least one optical
excitation light wavelength on to the optical axis wherein the at least one collimating optical element comprises at least
one optical prism adaptable to impinge the at least one optical excitation light wavelength on to the optical axis through
the outer circumference of the objective lens;

wherein the objective lens is adaptable to transmit emission light wavelength through the inner circumference of the objective
lens; and

said TIRFM illuminator apparatus not including a dichroic mirror for impinging the at least one optical excitation light wavelength
on to the optical axis.