US Patent No. 9,146,212

THREAD-BASED MICROFLUIDIC GUIDING SYSTEM


Patent No. 9,146,212
Issue Date September 29, 2015
Title Thread-based Microfluidic Guiding System
Inventorship Che-hsin Lin, Kaohsiung (TW)
Yi-chi Wei, Kaohsiung (TW)
Lung-min Fu, Kaohsiung (TW)
Yu-An Yang, Kaohsiung (TW)
Assignee NATIONAL SUN YAT-SEN UNIVERSITY, Kaohsiung (TW)

Claim of US Patent No. 9,146,212

1. A thread-based microfluidic guiding system, comprising:
a substrate;
a first fiber thread disposed on the substrate and sequentially having a sample fluid injection end, a sample fluid transmission
section, a first turning portion, a mixture fluid transmission section, and a first waste fluid end, wherein the sample fluid
transmission section is turned and coupled to the mixture fluid transmission section through the first turning portion;

a second fiber thread disposed on the substrate and sequentially having a buffer fluid injection end, a buffer fluid transmission
section, a second turning portion, a waste fluid transmission section, and a second waste fluid end, wherein the buffer fluid
transmission section is turned and coupled to the waste fluid transmission section through the second turning portion, and
the first turning portion crosses and contacts the second turning portion;

a working electrode disposed on the substrate and contacting the mixture fluid transmission section to form a detection area
which is close to the first waste fluid end;

a counter electrode which is disposed on the substrate and in contact with the mixture fluid transmission section, close to
the working electrode, and located between the working electrode and the first waste fluid end;

a reference electrode which is disposed on the substrate and in contact with the mixture fluid transmission section, and located
between the working electrode and the counter electrode; and

a decoupler electrode which is disposed on the substrate and in contact with the mixture fluid transmission section, close
to the working electrode, and located between the first turning portion and working electrode;

wherein each of the working electrode, the counter electrode, the reference electrode and the decoupler electrode has a flat
top surface parallel to a top surface of the substrate, and a groove configured as a depression in the flat top surface and
having a plurality of interior wall surfaces for contacting with the mixture fluid transmission section.