US Patent No. 10,920,280

GENOME CAPTURE AND SEQUENCING FOR COMPREHENSIVE CHROMATIN STRUCTURE MAPS IN COMPLEX GENOMES AND CANCER PROGRESSION


Patent No. 10,920,280
Issue Date February 16, 2021
Title Genome Capture And Sequencing For Comprehensive Chromatin Structure Maps In Complex Genomes And Cancer Progression
Inventorship Jonathan H. Dennis, Tallahassee, FL (US)
Assignee The Florida State University Research Foundation, Inc., Tallahassee, FL (U...

Claim of US Patent No. 10,920,280

1. A method of early detection of a grade one tumor in a patient via analysis of chromatin structure, and dysregulation thereof, in a genome of said patient, comprising the steps of:collecting a first sample from a first tissue of said patient, said first sample of said patient not being carcinogenic;
collecting a second sample from a second tissue of said patient, said second sample of said patient being carcinogenic;
targeting and capturing less than a 5% region of said genome in each of said first and second samples of said stage one subject, said captured region containing transcription start sites in said genome;
quantitatively measuring nucleosome distribution in said first sample within a first predetermined range of base pairs flanking a transcription start site in said first sample, wherein said quantitative measurement is performed after capturing said first predetermined range of base pairs in an Ataxia-Telangiesctasia Mutated (ATM) gene in said first sample;
quantitatively measuring nucleosome distribution in said second sample within a second predetermined range of base pairs flanking a transcription start site in said first sample, wherein said quantitative measurement is performed after capturing said second predetermined range of base pairs in an ATM gene in said second sample;
evaluating levels of nucleosome distribution between said first sample and said second sample, wherein a difference of nucleosome distribution between said levels of nucleosome distribution of 10% or higher indicates said second sample being carcinogenic, whereby said difference is not prevalent in later grade subjects.