US Patent No. 10,806,782

HAND, FOOT, AND MOUTH VACCINES AND METHODS OF MANUFACTURE AND USE THEREOF


Patent No. 10,806,782
Issue Date October 20, 2020
Title Hand, Foot, And Mouth Vaccines And Methods Of Manufacture And Use Thereof
Inventorship Subash Chandra Das, Middleton, WI (US)
Joseph David Santangelo, Helios (SG)
Dan Thomas Stinchcomb, Fort Collins, CO (US)
Jorge E. Osorio, Mount Horeb, WI (US)
Assignee Takeda Vaccines, Inc., Deerfield, IL (US)

Claim of US Patent No. 10,806,782

1. A hand, foot, and mouth immunogenic composition comprising one or more antigens from a chemically-inactivated enterovirus 71 (EV71), wherein the one or more antigens comprise the VP1 polypeptide of EV71, wherein the VP1 polypeptide comprises four mutations comprising:(i) a valine to methionine substitution at position 7 of SEQ ID NO: 1, or at a position corresponding to position 7 of SEQ ID NO: 1 when aligned to SEQ ID NO: 1 using a pairwise alignment algorithm;
(ii) an aspartic acid to asparagine substitution at position 14 of SEQ ID NO: 1, or at a position corresponding to position 14 of SEQ ID NO: 1 when aligned to SEQ ID NO: 1 using a pairwise alignment algorithm;
(iii) a glutamic acid to glutamine substitution at position 145 of SEQ ID NO: 1, or at a position corresponding to position 145 of SEQ ID NO: 1 when aligned to SEQ ID NO: 1 using a pairwise alignment algorithm; and
(iv) an asparagine to aspartic acid substitution at a position corresponding to position 282 of SEQ ID NO: 1 when aligned to SEQ ID NO: 1 using a pairwise alignment algorithm.