US Patent No. 10,656,117

NANOPORE-BASED DETERMINATION OF PROTEIN CHARGE, SHARP, VOLUME, ROTATIONAL DIFFUSION COEFFICIENT, AND DIPOLE MOMENT


Patent No. 10,656,117
Issue Date May 19, 2020
Title Nanopore-based Determination Of Protein Charge, Sharp, Volume, Rotational Diffusion Coefficient, And Dipole Moment
Inventorship Michael Mayer, Ann Arbor, MI (US)
Erik Yusko, Seattle, WA (US)
Assignee THE REGENTS OF THE UNIVERSITY OF MICHIGAN, Ann Arbor, MI (US)

Claim of US Patent No. 10,656,117

1. A method of detecting a target biomolecule in a solution comprising a plurality of different biomolecules, the method comprising:measuring an electrical parameter as a function of time between two liquid compartments separated by and fluidically coupled through a synthetic nanopore upon translocation of individual biomolecules of the plurality of different biomolecules in a plurality of translocation events from one liquid compartment through the synthetic nanopore to the other liquid compartment;
detecting the translocation events by recognizing a change in the electrical parameter relative to a baseline;
collecting values of intra-event values ?I(t);
deriving a parameter selected from the group consisting of the volume ?, the length to diameter ratio m, the rotational diffusion coefficient DR, and the dipole moment ? for each individual biomolecule of the plurality from the intra-event values ?I(t);
comparing the parameter for each individual biomolecule of the plurality of different biomolecules to a corresponding known parameter for the target biomolecule; and
determining the presence of the target biomolecule in the solution when the parameter for an individual biomolecule of the plurality of different biomolecules matches the corresponding known parameter for the target biomolecule.