1. A method for removing aggregates from a solution with high aggregate content, comprising the steps of:a) applying a solution comprising the polypeptide in monomeric form and in aggregated form, wherein the aggregated form is 20% or more as determined by size exclusion chromatography of the total. polypeptide in the solution, to a mixed-mode Chromatography material. and recovering the polypeptide from the mixed-mode chromatography material, wherein the polypeptide recovered from the mixed-mode chromatography material has a reduced aggregate content; and
b) applying the solution obtained in the previous step to a weak cation exchange chromatography material and recovering the polypeptide therefrom and thereby producing the monomeric polypeptide,
wherein the solution applied to the mixed-mode chromatography material and/or the weak cation exchange chromatography material has a pH value at least 0.2 pH units and at most 2.5 pH units above the isoelectric point of the polypeptide.