US Patent No. 10,428,322

HETEROLOGOUS EXPRESSION OF TERMITE CELLULASES IN YEAST


Patent No. 10,428,322
Issue Date October 01, 2019
Title Heterologous Expression Of Termite Cellulases In Yeast
Inventorship Elena E. Brevnova, Winchester, MA (US)
Vineet Rajgarhia, Dublin, CA (US)
Mark Mellon, Grantham, NH (US)
Anne Warner, Arlington, MA (US)
John McBride, Lyme, NH (US)
Chhayal Gandhi, Nashua, NH (US)
Erin Wiswall, Danbury, NH (US)
Assignee Lallemand Hungary Liquidity Management LLC, Budapest (HU)

Claim of US Patent No. 10,428,322

1. An isolated polynucleotide comprising a nucleic acid which encodes the mature endoglucanase of SEQ ID NO: 38, wherein said nucleic acid is codon-optimized for expression in a yeast strain wherein at least one nucleotide within a sequence of 4, 5, 6, 7, 8, 9, or 10 consecutive A, T, C or G nucleotides is replaced with a different nucleotide, wherein the nucleotide replacement does not alter the amino acid sequence encoded by the polynucleotide and wherein the nucleotide replacement creates a codon that is the second most frequently used codon to encode an amino acid in the yeast strain.