US Patent No. 10,428,319

ENGINEERED CAS9 NUCLEASES


Patent No. 10,428,319
Issue Date October 01, 2019
Title Engineered Cas9 Nucleases
Inventorship Barrett Ethan Steinberg, Cambridge, MA (US)
Derek Cerchione, Cambridge, MA (US)
Assignee EDITAS MEDICINE, INC., Cambridge, MA (US)

Claim of US Patent No. 10,428,319

1. A polypeptide having nuclease activity, comprising an amino acid sequence having at least 90% identity to SEQ ID NO:13, wherein the polypeptide comprises an amino acid substitution at two or more of the following positions of SEQ ID NO:13: D23, T67, Y128, and D1251.