US Patent No. 10,138,512

NUCLEIC ACID PROCESSING OF A NUCLEIC ACID FRAGMENT WITH A TRIAZOLE LINKAGE


Patent No. 10,138,512
Issue Date November 27, 2018
Title Nucleic Acid Processing Of A Nucleic Acid Fragment With A Triazole Linkage
Inventorship Afaf H. El-Sagheer, Hampshire (GB)
Tom Brown, Hampshire (GB)
Assignee ATDBIO LIMITED, Southhampton (GB)

Claim of US Patent No. 10,138,512

1. A method of nucleic acid processing comprising:providing an adapted nucleic acid fragment comprising a nucleic acid fragment linked at its 3?-end to a 3?-adapter molecule by a triazole linkage comprising a 1,2,3-triazole group, wherein the 3?-adapter molecule comprises RNA, DNA, a nucleic acid analogue or combinations thereof;
annealing a primer to the adapted nucleic acid fragment, wherein the primer anneals to a region of the 3?-adapter molecule; and
processing the adapted nucleic acid fragment by transcribing at least a portion of the adapted nucleic acid fragment with reverse transcriptase, wherein said portion of the adapted nucleic acid fragment transcribed with reverse transcriptase comprises said triazole linkage.