US Patent No. 10,138,502

METHOD FOR PRODUCING OIL CONTAINING POLYUNSATURATED FATTY ACID USING LIPASE


Patent No. 10,138,502
Issue Date November 27, 2018
Title Method For Producing Oil Containing Polyunsaturated Fatty Acid Using Lipase
Inventorship Hideo Ikemoto, Hachioji (JP)
Nobushige Doisaki, Hachioji (JP)
Yasuo Umehara, Hakodate (JP)
Assignee NIPPON SUISAN KAISHA, LTD., Tokyo (JP)

Claim of US Patent No. 10,138,502

1. A method for lowering saturated fatty acid content in a glyceride containing a polyunsaturated fatty acid, the method comprising:concentrating the polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to hydrolyze the glyceride containing the polyunsaturated fatty acid, wherein the glyceride containing the polyunsaturated fatty acid is a marine animal oil glyceride or a microorganism oil glyceride;
wherein the polyunsaturated fatty acid is a fatty acid having at least 20 carbon atoms and having at least 3 double bonds,
wherein the hydrolysis by the lipase is performed in the presence of 10 to 200 weight percent of water based on a total weight of the glyceride containing the polyunsaturated fatty acid, and at a temperature from 10° C. to 20° C.,
wherein the lipase is derived from a microorganism selected from the group consisting of Candida cylindracea and Alcaligenes sp.; and
wherein the saturated fatty acid content in the resulting glyceride is less than the saturated fatty acid content in the glyceride prior to the concentrating.