US Patent No. 10,137,186

COMPOSITIONS AND METHODS FOR LIVE, ATTENUATED ALPHAVIRUS FORMULATIONS


Patent No. 10,137,186
Issue Date November 27, 2018
Title Compositions And Methods For Live, Attenuated Alphavirus Formulations
Inventorship Dan T. Stinchcomb, Enumclaw, WA (US)
Jill A. Livengood, Fort Collins, CO (US)
Laszlo Varga, Fort Collins, CO (US)
Assignee Takeda Vaccines, Inc., Cambridge, MA (US)

Claim of US Patent No. 10,137,186

1. A live, attenuated alphavirus virus composition comprising:one or more live, attenuated alphaviruses, wherein the live, attenuated alphaviruses are selected from the group consisting of chikungunya (CHIK) virus, o'nyong'nyong virus, Eastern equine encephalitis, Western equine encephalitis, and Venezuelan equine encephalitis;
10.0 mM to 20.0 mM HEPES buffer;
one or more carbohydrate agents having a concentration from 1.0% to 15% (w/v) selected from the group consisting of: trehalose, sucrose, mannitol, sorbitol, and galactose; and
0.1% to 1.0% (w/v) gelatin but does not contain human serum albumin (HSA), wherein the composition stabilizes the live attenuated alphaviruses.