US Pat. No. 9,296,984

TISSUE PROCESSING APPARATUS AND METHOD FOR PROCESSING ADIPOSE TISSUE

THE GID GROUP, INC., Lou...

1. An apparatus for processing human biological material containing stringy tissue, the apparatus comprising;
a container having an internal containment volume, the internal containment volume including a tissue retention volume and
a filtrate volume;

a filter disposed within the internal containment volume with the tissue retention volume on one side of the filter and the
filtrate volume on another side of the filter with the tissue retention volume and the filtrate volume being in fluid communication
through the filter;

an inlet port in fluid communication with the tissue retention volume and configured to access the tissue retention volume
for introducing human biological material into the tissue retention volume;

a suction port in fluid communication with the filtrate volume and configured to access the filtrate volume for suctioning
material from the filtrate volume;

a tissue collector disposed in the tissue retention volume and rotatable relative to the container in at least a first direction
of rotation about an axis of rotation, the tissue collector including at least one toothed member that sweeps through a portion
of the tissue retention volume when the tissue collector is rotated in the first direction, the toothed member being configured
with a plurality of teeth to collect and retain stringy tissue when the tissue collector is rotated in the first direction
in contact with human biological material containing stringy tissue disposed in the tissue retention volume; and

wherein, each said toothed member has a first end located radially toward the axis and a second end located radially away
from the axis, each said toothed member includes between the first end and the second end at least three teeth and an open
space between the teeth of each pair of adjacent said teeth and the teeth project toward a leading side of the toothed member
when the tissue collector is rotated in the first direction.

US Pat. No. 10,138,457

TISSUE PROCESSING APPARATUS AND METHOD FOR PROCESSING ADIPOSE TISSUE

The GID Group, Inc., Lou...

1. A method for processing biological material containing stringy tissue, the method comprising;providing a container having
at least one inlet port,
an internal containment volume, the internal containment volume including a tissue retention volume and a filtrate volume separated from the tissue retention volume by a filter, and
a tissue collector disposed in the tissue retention volume and rotatable relative to the container in a direction of rotation about an axis of rotation, the tissue collector including multiple teeth positioned on the tissue collector;
washing a biological material contained in the tissue retention volume by adding a wash liquid to the tissue retention volume and allowing the wash liquid and contaminants to pass through the filter from the tissue retention volume to the filtrate volume; and
rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect stringy material on the tissue collector.

US Pat. No. 9,260,697

APPARATUS AND METHODS RELATING TO COLLECTING AND PROCESSING HUMAN BIOLOGICAL MATERIAL CONTAINING ADIPOSE

THE GID GROUP, INC., Lou...

1. An apparatus useful for collection of human biological material and post-collection processing of collected material, the
apparatus being orientable in a collection orientation, as oriented in the collection orientation the apparatus comprising:
a filter;
a container having an internal containment volume, the internal containment volume comprising:
a tissue retention volume and a filtrate volume separated by the filter;
a collection volume within the filtrate volume, the collection volume having a bottom elevation corresponding to a bottom
elevation of the filtrate volume and a top elevation that is lower than the bottom elevation of the tissue retention volume;

a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located above the
collection volume;

an inlet port in fluid communication with the tissue retention volume and configured for introducing human biological material
comprising adipose directly into the tissue retention volume;

a suction port in fluid communication with the filtrate volume and providing access to the filtrate volume for suctioning
from the filtrate volume components passing through the filter from the tissue retention volume to the filtrate volume;

a mixing device disposed at least in part within the tissue retention volume for mixing contents within the tissue retention
volume, the mixing device comprising a rotatable shaft extending from outside of the internal containment volume to inside
of the internal containment volume; and

a lumen extending through the shaft and having a proximal end located outside of the internal containment volume and a distal
end located within the internal containment volume, thereby providing access from outside of the internal containment volume
to inside of the internal containment volume.

US Pat. No. 9,206,387

METHOD AND APPARATUS FOR PROCESSING ADIPOSE TISSUE

THE GID GROUP, INC., Lou...

1. An apparatus for collection of human biological material and post-collection processing of collected material, the apparatus
being orientable in an access orientation, as oriented in the access orientation the apparatus comprising:
a filter;
a container having an internal containment volume having a volume in a range of from 100 cubic centimeters to 1300 cubic centimeters,
the internal containment volume comprising:

a tissue retention volume and a filtrate volume separated by the filter;
a collection volume within the filtrate volume, the collection volume having a bottom elevation corresponding to a bottom
elevation of the filtrate volume and a top elevation that is lower than the bottom elevation of the tissue retention volume;

a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located below the
bottom elevation of the tissue retention volume;

an inlet port in fluid communication with the tissue retention volume and configured for introducing human biological material
directly into the tissue retention volume;

a suction port in fluid communication with the filtrate volume and providing access to the filtrate volume for suctioning
from the filtrate volume components passing through the filter from the tissue retention volume to the filtrate volume; and

an extraction port in fluid communication with the internal containment volume and configured for removing processed biological
material from the internal containment volume;

wherein each of the inlet port, the suction port and the extraction port is configured for access therethrough from above
the container into the internal containment volume;

a mixing device disposed at least in part within the tissue retention volume for mixing contents within the tissue retention
volume, wherein the mixing device comprises a rotatable shaft extending from outside of the internal containment volume to
inside of the internal containment volume, wherein the shaft is rotatable about an axis that extends through the collection
volume, the apparatus further comprising:

a lumen extending through the rotatable shaft and having a proximal end located outside of the internal containment volume
and a distal end located within the internal containment volume, thereby providing access from outside of the internal containment
volume to inside of the internal containment volume.

US Pat. No. 9,909,095

TISSUE PROCESSING APPARATUS WITH FILTER PIERCEABLE TO REMOVE PRODUCT AND METHOD FOR PROCESSING ADIPOSE TISSUE

The GID Group, Inc., Lou...

1. An apparatus useful for collection of human biological material and for post-collection processing of collected material
including enzymatic digestion and post-digestion centrifuging of the apparatus to prepare in the apparatus a concentrate product
comprising stromal vascular fraction cells, the apparatus being orientable in a collection orientation for collection of human
biological material comprising adipose during a lipoplasty procedure, as oriented in the collection orientation the apparatus
comprising:
a filter;
a container having an internal containment volume, the internal containment volume comprising:
a tissue retention volume and a filtrate volume separated by the filter;
a collection volume within the filtrate volume and configured to collect the concentrate product during the centrifuging,
the collection volume having a bottom elevation corresponding to a bottom elevation of the filtrate volume and a top elevation
that is lower than the bottom elevation of the tissue retention volume; and

a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located above the
collection volume;

an inlet port in fluid communication with the tissue retention volume and configured for introducing human biological material
directly into the tissue retention volume;

a suction port in fluid communication with the filtrate volume and providing access to the filtrate volume for suctioning
from the filtrate volume components passing through the filter from the tissue retention volume to the filtrate volume; and

an extraction port in fluid communication with the internal containment volume and providing access to the collection volume;
wherein:
the extraction port is configured for access therethrough from above the container into the internal containment volume;
the apparatus is configured for advancing a hollow member through the extraction port to access the collection volume with
an advancing tip of the hollow member; and

the extraction port is located above a portion of the filter, so that the advancing tip of the hollow member pierces through
the filter when the tip of the hollow member is advanced from the extraction port into the collection volume.

US Pat. No. 9,907,883

METHOD FOR PROCESSING CANCELLOUS BONE MATERIAL AND RELATED PRODUCTS, METHODS AND USES

The GID Group, Inc., Lou...

1. A method for processing cancellous bone material initially comprising cancellous bone and associated blood, the method
comprising;
performing processing operations using a portable container apparatus;
the portable container apparatus having a filter disposed in an internal containment volume of the portable container apparatus
and the internal containment volume includes a retentate volume on one side of the filter and a filtrate volume on another
side of the filter;

the processing operations comprising:
washing, wherein the washing comprises adding aqueous wash liquid to the portable container apparatus to contact cancellous
bone material disposed within the retentate volume and removing from the filtrate volume at least a majority of the aqueous
wash liquid together with at least some blood washed from the cancellous bone material;

after the washing, digesting, wherein the digesting comprises adding to the portable container apparatus a digestion medium
comprising collagenase enzyme to contact the digestion medium with at least a washed portion of the cancellous bone material
disposed in the retentate volume; and

after the digesting, centrifuging the portable container apparatus having disposed within the internal containment volume
at least a digested portion of the cancellous bone material, and during the centrifuging forming in the internal containment
volume density-separated phases, the density-separated phases including lower-density material phases and a higher-density
pellet phase concentrated in stromal vascular fraction cells from the cancellous bone material;
and wherein:
the apparatus has an access orientation;
the internal containment volume includes a pellet well disposed in a bottom portion of the filtrate volume below a bottom
elevation of the filter and accessible only from above when the apparatus is in the access orientation;

the filtrate volume includes a lower tapered portion below a bottom elevation of the filter and above a top elevation of the
pellet well;

the lower tapered portion of the filtrate volume is defined by internal wall surfaces of the container that are inclined relative
to horizontal at a maximum angle of no larger than 60° when the container is in the access orientation; and

at least a portion of the pellet well is defined by a wall surface of the container inclined relative to horizontal at an
angle that is larger than the maximum angle of the lower tapered portion when the apparatus is in the access orientation.

US Pat. No. 9,909,094

TISSUE PROCESSING APPARATUS WITH MIXING DEVICE AND METHOD FOR PROCESSING ADIPOSE TISSUE

The GID Group, Inc., Lou...

1. An apparatus useful for collection of human biological material comprising adipose and post-collection processing of collected
material, the apparatus being orientable in a collection orientation, as oriented in the collection orientation the apparatus
comprising:
a filter;
a container having an internal containment volume, the internal containment volume comprising:
a tissue retention volume and a filtrate volume separated by the filter;
a collection volume within the filtrate volume, the collection volume having a bottom elevation corresponding to a bottom
elevation of the filtrate volume and a top elevation that is lower than the bottom elevation of the tissue retention volume;
and

a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located above the
collection volume and with at least a portion of the tissue retention volume being within the tapered portion;

an inlet port in fluid communication with the tissue retention volume and configured for introducing the human biological
material directly into the tissue retention volume;

a suction port in fluid communication with the filtrate volume and providing access to the filtrate volume for suctioning
from the filtrate volume components passing through the filter from the tissue retention volume to the filtrate volume; and

a mixing device disposed at least in part within the tissue retention volume for mixing contents within the tissue retention
volume, wherein the mixing device comprises a rotatable shaft extending from outside of the internal containment volume to
inside of the internal containment volume and comprises at least one mixing member that

is disposed in the tissue retention volume with at least a portion disposed in the tapered portion of the internal containment
volume and

is connected with the shaft to move through the tissue retention volume when the shaft is rotated; and
wherein, the mixing device comprises a filter contact member that moves when the shaft is rotated, the filter contact member
contacting the filter at least periodically when the shaft is rotated.

US Pat. No. 9,950,015

TISSUE PROCESSING APPARATUS WITH FLUID SUCTION FEATURES AND METHODS RELATING TO COLLECTING AND PROCESSING HUMAN BIOLOGICAL MATERIAL

The GID Group, Inc., Lou...

1. An apparatus for collection of human biological material and post-collection processing of collected material, the apparatus being orientable in a collection orientation for collection of human biological material, as oriented in the collection orientation the apparatus comprising:a filter;
a container having an internal containment volume, the internal containment volume comprising:
a tissue retention volume and a filtrate volume separated by the filter;
a collection volume within the filtrate volume, the collection volume having a bottom elevation corresponding to a bottom elevation of the filtrate volume and a top elevation that is lower than the bottom elevation of the tissue retention volume;
a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located above the collection volume;
an inlet port in fluid communication with the tissue retention volume and configured for introducing human biological material comprising adipose directly into the tissue retention volume;
a suction port in fluid communication with the filtrate volume and providing access to the filtrate volume for suctioning from the filtrate volume components passing through the filter from the tissue retention volume to the filtrate volume; and
wherein:
the suction port is in fluid communication with the tapered portion of the internal containment volume through a conduit providing fluid communication from the suction port to a location within the filtrate volume that is within the tapered portion of the internal containment volume;
the conduit extends through the filtrate volume from adjacent the suction port to the location within the filtrate volume;
the suction port is located above the tapered portion of the internal containment volume and above the collection volume;
the location is within the collection volume;
the container comprises:
a fluid containment shell with an internal cavity portion forming at least a part of the internal containment volume, the internal cavity portion being open to above; and
a lid attached to the shell and disposed to cover from above the internal cavity portion;
the suction port passes through the lid;
the shell comprises an upper portion having a first wall surface portion defining a corresponding upper portion of the internal containment volume, wherein substantially all of the first wall surface portion has an incline relative to horizontal of at least 75° ;
the shell comprises a lower portion located below the upper portion and having a second wall surface portion defining a corresponding lower portion of the internal containment volume, the lower portion comprising a tapered wall surface portion defining the tapered portion of the internal containment volume, substantially all of the tapered wall surface portion having an incline relative to horizontal in a range of from 30° to 60°; and
the conduit extends from the suction port through the upper portion of the internal containment volume and into the tapered portion within the lower portion of the internal containment volume.

US Pat. No. 10,336,980

TISSUE PROCESSING APPARATUS AND METHOD FOR PROCESSING ADIPOSE TISSUE

The GID Group, Inc., Lou...

1. A portable apparatus with a container for multi-step processing of human biological material containing stringy tissue including washing, enzymatic digestion and centrifuging to prepare a cell concentrate in the container in the form of a pellet phase formed during the centrifuging, the apparatus being orientable in an access orientation and the apparatus comprising:the container having an internal containment volume including a tissue retention volume and a filtrate volume;
a filter disposed within the internal containment volume with the tissue retention volume on one side of the filter and the filtrate volume on another side of the filter with the tissue retention volume and the filtrate volume being in fluid communication through the filter;
an inlet port in fluid communication with the tissue retention volume and configured to access the tissue retention volume for introducing human biological material into the tissue retention volume;
a suction port in fluid communication with the filtrate volume and configured to access the filtrate volume for suctioning material from the filtrate volume;
wherein:
the internal containment volume includes a pellet well disposed in a bottom portion of the filtrate volume below a bottom elevation of the filter and accessible only from above when the apparatus is in the access orientation;
the pellet well has a volume in a range of from 0.3 cubic centimeter to 3 cubic centimeters;
when the apparatus is in the access orientation, the pellet well has at least one portion with a vertical length of 0.5 centimeter, a maximum horizontal cross-dimension of no larger than 10 millimeters and a minimum horizontal cross-dimension of no smaller than 1.5 millimeters; and
the internal containment volume comprises an available processing volume with a portion of the available processing volume being in the tissue retention volume and the volume of the pellet well is in a range of from 0.2 percent to 2 percent of the portion of the available processingvolume in the tissue retention volume;
wherein the available processing volume is in a range of from 20 cubic centimeters to 400 cubic centimeters.