US Pat. No. 9,589,099

DETERMINATION OF GENE EXPRESSION LEVELS OF A CELL TYPE

The Chinese University of...

1. A method for measuring a genomic expression in a first subpopulation of cells of a sample of a cell mixture, the method
comprising:
identifying a plurality of subpopulation informative genes including:
at least one subpopulation target gene; and
at least one subpopulation reference gene having a lower biological variation relative to the subpopulation target gene, each
of the plurality of subpopulation informative genes identified as having at least a predetermined percentage of its transcripts
in one or more calibration samples of the cell mixture contributed by the cells of the first subpopulation, the cell mixture
containing a plurality of different subpopulations of cells, the predetermined percentage being equal to or greater than 50%;

performing one or more reactions involving the cell mixture sample;
measuring signals corresponding to the plurality of subpopulation informative genes from the one or more reactions involving
the cell mixture sample, wherein the signals are proportional to amounts of transcripts of the plurality of subpopulation
informative genes in the cell mixture sample;

receiving, at a computer system, the signals obtained from the measurement of the cell mixture sample;
using the signals to determine, with the computer system, a first amount of transcripts of the at least one subpopulation
target gene in the cell mixture sample and a second amount of transcripts of the at least one subpopulation reference gene
in the cell mixture sample; and

calculating a parameter that is a relative value of the first amount to the second amount, the parameter providing a measurement
of an amount of genomic expression of the subpopulation target gene in the first subpopulation of cells of the cell mixture
sample.

US Pat. No. 9,298,050

TERAHERTZ IN-PLANE AND TERAHERTZ OUT-OF-PLANE SWITCHING LIQUID CRYSTAL DEVICES

The Hong Kong University ...

1. A liquid crystal cell, comprising:
a first window transparent at terahertz (THz) frequencies between 0.1 THz to 10.0 THz;
a second window transparent at THz frequencies between 0.1 THz to 10.0 THz;
a liquid crystal layer, disposed between the first and second windows;
a first plurality of in-plane switching electrodes, configured to generate in-plane electric fields over the liquid crystal
layer, disposed between the liquid crystal layer and the first window;

a second plurality of in-plane switching electrodes, configured to generate in-plane electric fields over the liquid crystal
layer, disposed between the liquid crystal layer and the second window;

a first plurality of out-of-plane switching electrodes, configured to generate out-of-plane electric fields that are perpendicular
to the liquid crystal layer, disposed between the liquid crystal layer and the first window; and

a second plurality of out-of-plane switching electrodes, configured to generate out-of-plane electric fields that are perpendicular
to the liquid crystal layer, disposed between the liquid crystal layer and the second window;

wherein the first and second pluralities of in-plane switching electrodes and the first and second pluralities of out-of-plane
switching electrodes are wire-grid electrodes and are configured to be controlled independently to tune optical properties
of the liquid crystal cell.

US Pat. No. 9,322,778

OPTICAL SENSING APPARATUS AND A METHOD FOR DETECTING CHARACTERISTICS OF A SAMPLE

THE CHINESE UNIVERSITY OF...

1. An optical sensing apparatus comprising:
a broadband source configured to generate a first light beam containing a p-polarized component and an s-polarized component;
an interferometer configured to direct the p-polarized component and the s-polarized component to a first path and a second
path, respectively, wherein the first path has a different length than the second path such that the p-polarized component
and the s-polarized component are reflected and then recombined to provide a second light beam with interference between the
p-polarized component and the s-polarized component;

a reference device configured to receive a part of the second light beam to extract a reference optical characteristic variation
induced by the interference;

a surface plasmon resonance (SPR) sensor configured to receive the other part of the second light beam and to introduce an
SPR effect associated with a sample to the second light beam, such that a third light beam with a probe optical characteristic
variation induced by the SPR effect is generated;

a polarizer configured to receive the third light beam to generate a probe light beam;
a lens placed in the back of the polarizer and configured to couple the probe light beam to an analyzer, such that the analyzer
extracts the probe optical characteristic variation induced by the SPR effect of the probe light beam; and

a processing module configured to determine characteristics of the sample by comparing the reference optical characteristic
variation and the probe optical characteristic variation to determine characteristics of the sample.

US Pat. No. 9,713,603

PRODRUG OF GREEN TEA EPIGALLOCATECHIN-3-GALLATE (PRO-EGCG) FOR USE IN THE TREATMENT OF ENDOMETRIOSIS

The Hong Kong Polytechnic...

1. A method of treating endometriosis or a related indication comprising administering to a subject in need thereof an effective
amount of Pro-EGCG having the formula:

US Pat. No. 9,210,451

REPLICATION DECISION IN P2P VOD SYSTEMS

The Chinese University of...

1. A system for making a replication decision in a Peer-to-Peer Video-on-Demand (P2P VoD) architecture, the system comprising:
a plurality of peers;
a server maintaining a replication indicator for each of a plurality of videos distributed over the peers, wherein the replication
indicator for each video indicates a redundancy, inadequacy or balance of replication of the video;

wherein each of the peers reports to the server first information regarding one or more first videos of the plurality of videos
which are stored in the peer and reports second information that includes a downloading rate of a second video of the plurality
of videos which has been viewed at the peer; and

wherein the server determines, according to the first information, the second information and the replication indicators for
the one or more first videos and the second video, whether the peer is to replace at least one of the one or more first videos
with the second video when the peer does not have enough space for storing the second video; and

wherein the server determines the replication indicator for the second video using the downloading rates for the second video
reported to the server by all the peers at which the second video has been viewed.

US Pat. No. 9,447,064

HETEROCYCLIC COMPOUND PREPARING

THE CHINESE UNIVERSITY OF...

1. A process for selectively producing a heterocyclic compound from a diene in the presence of a buffer and a catalyst, wherein
the catalyst is represented by the general structure [carbeneLMRL]AL, wherein M represents a transition metal selected from the group consisting of Fe, Co, Ni, Ru, Rh, Pd, Os, Ir, Pt, Cu, Ag,
Au, Ti, Zr and Sc; R represents H or a carbon fragment; A represents a halogen, sulfonate or other non-nucleophilic ion; and
L independently represents an integer wherein 1?L?4;
the diene is represented by the formula
and
the heterocyclic compound is represented by the formula

wherein R1, R2, R3 and R4 are independently selected from the group consisting of hydrogen,

optionally substituted alkyl, optionally substituted aryl, and optionally substituted alkylene;
X is selected from the group consisting of oxygen, nitrogen, sulfur, and silicon;
Y and Z respective represent a spacer and are independently selected from the group consisting of optionally substituted alkyl,
optionally substituted aryl, optionally substituted alkylene, an optionally substituted cycloalkyl, oxygen, nitrogen, sulfur,
silicon, and metalloid atoms; or

two or more members selected from the group consisting of R1, R2, R3, R4, X, Y and Z are linked; and

n and m are each an integer respectively, wherein 0?n?50, and 0?m?50.

US Pat. No. 9,056,916

METHOD AND ASSAY KIT FOR DETECTION OF TOXICITY INDUCED BY PYRROLIZIDINE ALKALOIDS

THE CHINESE UNIVERSITY OF...

1. An antibody, comprising a binding site which specifically recognizes a pyrrole moiety conjugated to a cellular macromolecule,
and being raised in a mammal administered with a synthetic immunogen comprising the pyrrole moiety as hapten conjugated to
a carrier protein, wherein the pyrrole moiety has the structure

and the antibody has no cross-reactivity with monocrotaline and retrorsine.
US Pat. No. 9,371,566

DIAGNOSTIC METHOD

The Chinese University of...

1. A method for the detection or monitoring of hepatocellular carcinoma or nasopharyngeal carcinoma in a biological sample
selected from blood, plasma, serum, saliva, and urine from an individual, or for assessing the susceptibility of said individual
to developing hepatocellular carcinoma or nasopharyngeal carcinoma, which comprises
(a) subjecting the sample, or nucleic acid extracted therefrom, to a methylation-sensitive restriction enzyme;
(b)(i) amplifying a target sequence comprising residues 1142 to 1269 of SEQ ID NO:1 from said sample, or said nucleic acid
extracted therefrom, with a primer comprising the sequence shown in SEQ ID NO: 2 and a primer comprising the sequence shown
in SEQ ID NO: 3, and (ii) detecting residues 1142 to 1269 of SEQ ID NO:1 with a detectably-labelled probe comprising the sequence
shown in SEQ ID NO: 4, and

(c)(i) amplifying a ?-actin control sequence from said sample, or said nucleic acid extracted therefrom, with a primer comprising
the sequence shown in SEQ ID NO: 5 and a primer comprising the sequence shown in SEQ ID NO: 6, and (ii) detecting the amplified
?-actin control sequence with a detectably-labelled probe comprising the sequence shown in SEQ ID NO: 7,

wherein detecting amplified target sequence in step (b)(ii), while not detecting amplified control sequence in step (c)(ii),
indicates the detection of hepatocellular carcinoma or nasopharyngeal carcinoma, or susceptibility of developing hepatocellular
carcinoma or nasopharyngeal carcinoma, in the individual.

US Pat. No. 9,112,771

SYSTEM AND METHOD FOR CATCHING TOP HOSTS

The Chinese University of...

1. A system for catching top hosts from a plurality of hosts, comprising:
a filter configured to sample flows from the hosts and remove the flows that do not satisfy a constraint;
a tracker configured to record a first estimated flow count for each host and to determine a first set of hosts from the plurality
of hosts in terms of the estimated flow count; and

an estimator configured to determine a second estimated flow count for each of the determined hosts and select a second set
of hosts from the determined hosts based on the second estimated flow count,

wherein determining the second estimated flow count further comprises:
calculating a plurality of sums from a plurality of counters;
determining whether any of the sums overflow;
estimating the flow count based on the determined difference and a rate of sampling r if any of the sums are determined to
overflow; and

estimating the flow count based on counts in the plurality of counters, the calculated sums, the rate of sampling r, and a
threshold for filtering non-top hosts if any of the sums do not overflow in the estimating.

US Pat. No. 9,051,616

DIAGNOSING FETAL CHROMOSOMAL ANEUPLOIDY USING MASSIVELY PARALLEL GENOMIC SEQUENCING

The Chinese University of...

1. A method for determining the presence or absence of fetal aneuploidy from a plasma or serum sample from a pregnant female
subject, wherein the sample comprises cell-free nucleic acid molecules from the genome of both the female subject and a fetus,
comprising:
(a) performing random massively parallel DNA sequencing on at least a portion of the cell-free nucleic acid molecules to generate
random sequenced tags;

(b) aligning at least a portion of the sequenced tags obtained in step (a) to a reference genome;
(c) calculating a fractional representation of a first chromosome from sequenced tags aligned to the first chromosome and
sequenced tags aligning to one or more second chromosomes;

(d) comparing the fractional representation of the first chromosome to a cutoff value representing the expected fractional
representation in the absence of aneuploidy; and

(e) based on the comparison step of (d), determining whether fetal aneuploidy is present or absent.
US Pat. No. 9,512,480

DETERMINATION OF THE DEPTH COVERAGE OF THE FETAL GENOME

The Chinese University of...

1. A method of determining a first proportion of a fetal genome that has been sequenced from a biological sample obtained
from a pregnant female, the fetus having a father and a mother being the pregnant female, the father having a paternal genome
and the mother having a maternal genome, wherein the sample contains a mixture of maternal and fetal nucleic acids, the method
comprising:
receiving sequencing results of a sequencing of a plurality of nucleic acid molecules from the biological sample obtained
from the pregnant female;

analyzing the sequencing results, the analyzing for a nucleic acid molecule including:
identifying a location of the nucleic acid molecule in a human genome; and
determining a respective allele of the nucleic acid molecule;
determining a first plurality of loci, wherein the fetal genome is heterozygous at each loci of the first plurality such that
the fetal genome has a respective first and second allele at that loci, and wherein the maternal genome is homozygous at each
loci of the first plurality such that the maternal genome has two of the respective second allele at that loci, the first
allele being different than the second allele;

determining, with a computer system, a second proportion of loci of the first plurality of loci in which a respective first
allele is detected from the sequencing results;

based on the second proportion, determining the first proportion of the fetal genome that has been sequenced from the biological
sample;

determining that a threshold amount of sequencing has not been performed based on the first proportion of the fetal genome
that has been sequenced from the biological sample; and

performing more sequencing in response to the threshold amount of sequencing having not been reached.
US Pat. No. 9,394,570

MARKER FOR COLON CANCER AND METHOD FOR DETECTING COLON CANCER

The Chinese University of...

1. A method for assessing risk of colon cancer in a human patient, said method comprising the steps of:
(1) contacting genomic DNA isolated from a colon mucosa sample from the human patient with a bisulfite, wherein the bisulfite
converts unmethylated cytosines in DNA present in the sample to uracils;

(2) performing a polymerase chain reaction (PCR) to amplify a genomic DNA sequence comprising SEQ ID NO:1 using a primer consisting
of SEQ ID NO:4 and a primer consisting of SEQ ID NO:5;

(3) determining number of methylated cytosine-phosphate-guanine (CpGs) in the genomic sequence amplified in step (2);
(4) comparing the number of methylated CpGs with the number of methylated CpGs in the genomic sequence from a non-cancer colon
mucosa sample and processed through steps (1) to (3); and

(5) determining the human patient, whose colon mucosa sample contains more methylated CpGs in the genomic sequence determined
in step (3) compared to the number of methylated CpGs with the number of methylated CpGs in the genomic sequence from a noncancer
colon mucosa tissue sample and processed through steps (1) to (3), as having an increased risk of colon cancer compared with
a human subject not diagnosed with colon cancer.

US Pat. No. 9,284,611

EPIGENETIC BIOMARKER ZNF545 FOR DIAGNOSING AND PROGNOSIS OF GASTRIC CANCER

The Chinese University of...

1. A method for assessing time of survival from gastric cancer in a subject suffering from gastric cancer, comprising the
steps of:
(a) detecting methylation in a promoter region of the ZNF545 gene corresponding to SEQ ID NO: 1 or 2 by:
(i) treating genomic DNA of a sample taken from the subject with a bisulfite, wherein the sample is taken from non-cancer
tissue adjacent to the gastric cancer;

(ii) amplifying the treated genomic DNA by performing a polymerase chain reaction (PCR) with a primer selected from the group
consisting of SEQ ID NOs: 12, 13, 16, and 17;

(iii) detecting in the amplified DNA after stop (b) a methylated version of the promoter region of the ZNF545 gene; and
(b) determining the subject as having a shorter time of survival when a methylated version of the genomic DNA sequence is
detected in the sample, in comparison to another subject suffering from gastric cancer but whose non-cancer tissue adjacent
to gastric cancer is determined to contain no methylated version of the promoter region of the ZNF545 gene.

US Pat. No. 9,805,296

METHOD AND APPARATUS FOR DECODING OR GENERATING MULTI-LAYER COLOR QR CODE, METHOD FOR RECOMMENDING SETTING PARAMETERS IN GENERATION OF MULTI-LAYER QR CODE, AND PRODUCT COMPRISING MULTI-LAYER COLOR QR CODE

The Chinese University of...

27. An apparatus for generating a multi-layer color QR code from data to be encoded, comprising:
a processor; and
a memory;
the memory storing computer-readable instructions which when executed by the processor, cause the processor to perform operations,
the operations comprising:

partitioning the data to be encoded into a plurality of data blocks according to the number of layers and an error correction
level of each layer specified by a user;

breaking the data in all the data blocks into bits;
encoding the portioned data blocks into a plurality of monochrome QR codes independently, wherein each monochrome QR code
shares a same dimension;

shuffling the data blocks in each monochrome QR code in a bit-by-bit manner randomly or based on sizes of each monochrome
QR code;

combining all the monochrome QR codes to generate a multi-layer color QR code using a predefined color codebook; and
adding color constraints to spatial patterns,
wherein the adding of color constraints to spatial patterns comprises: coloring all finder patterns and alignment patterns
with a set of color to reduce false positives in a localization process.

US Pat. No. 9,425,886

NETWORK-CODING BUILDING BLOCKS AND DECOMPOSITION SCHEDULING BASED THEREON

The Chinese University of...

1. A data communication method comprising:
identifying a plurality of physical-layer network coding (PNC) atoms in a digital telecommunications network, the digital
telecommunications network comprising a plurality of nodes and at least one relay,

each of the plurality of PNC atoms comprising:
a transmission pattern attribute,
at least some of the plurality of nodes, and
the at least one relay in the network,
wherein each transmission pattern attribute of the plurality of PNC atoms cannot be decomposed to any other transmission pattern
attribute of the PNC atoms;

wherein each of the plurality of PNC atoms specifies how traffic flows between the plurality of nodes and the at least one
relay using the transmission pattern attribute;

generating a transmission schedule using the plurality of PNC atoms based at least in part on the transmission pattern attribute
of at least some of the PNC atoms; and

relaying traffic between the plurality of nodes and the at least one relay, using the transmission schedule.

US Pat. No. 9,601,647

CONVERTING INFRARED LIGHT INTO BROADBAND VISIBLE LIGHT AT HIGH EFFICIENCY USING LANTHANIDE-SENSITIZED OXIDES

The Chinese University of...

1. A doped oxide, comprising
A) an oxide host having:
i) a melting point greater than 1800 K; and
ii) a room temperature thermal conductivity lower than 45 W·m?1·K?1; and

B) a dopant uniformly distributed in the oxide host,
wherein the doped oxide is in the form of mesoporous microspheres having a diameter of about 10 to 50 ?m, wherein the surface
area of the mesoporous microspheres is about 1 to 200 m2/g, and wherein the mesoporous microspheres have a pore size of about 2 to 100 nm.

US Pat. No. 9,117,121

DETECTION OF DISEASE-RELATED RETINAL NERVE FIBER LAYER THINNING

The Chinese University of...

1. A method for identifying regions of abnormalities in a patient's eye, the method comprising:
receiving a plurality of images of the patient's eye, each image obtained at a different time, wherein each image of the plurality
of images is comprised of a plurality of pixels, each pixel indicating a time-varying characteristic of a particular location
in the patient's eye;

dividing each image into a plurality of pixel partitions, each pixel partition including one or more pixels;
identifying a plurality of pixel partition sets, each pixel partition set comprising a pixel partition from each image, wherein
the pixel partitions of the pixel partition set correspond to a common region in the patient's eye;

for each pixel partition set:
for each pixel partition of the pixel partition set:
determining a respective value for the respective common region of the pixel partition set, wherein the respective value corresponds
to the time-varying characteristic of the pixel partition at the time of the corresponding image;

calculating, with a computer system, a regression model from the respective values of the pixel partitions of the pixel partition
set, wherein the regression model comprises a rate of change value; and

determining whether the common region that corresponds to the pixel partition set exhibits at least one abnormality in the
patient's eye, based on whether the rate of change value of the regression model is more negative than a rate of age-related
change.

US Pat. No. 9,094,994

NETWORK-CODING BUILDING BLOCKS AND DECOMPOSITION SCHEDULING BASED THEREON

The Chinese University of...

16. A data communication method comprising:
identifying at least one relay in a digital telecommunications network communicatively connected to each of a plurality of
nodes in said network;

identifying a plurality of physical layer network coding (PNC) atoms comprising the at least one relay and at least some of
the plurality of nodes, the at least one PNC atom specifying how transmission traffic flows between the at least some of the
plurality of nodes and the at least one relay;

generating a transmission schedule, wherein the transmission schedule comprises a first instance of one of the PNC atoms;
and

relaying traffic across the plurality of nodes by instructing the plurality of nodes to transmit packet information according
to a first instance of the PNC atoms.

US Pat. No. 9,051,614

METHODS FOR ASSESSING LIVER PATHOLOGIES

The Chinese University of...

1. A method for detecting fatty liver, comprising the steps of:
(a) performing a reverse transcriptase-polymerase chain reaction (RT-PCR) to determine the concentration of albumin mRNA in
an acellular blood sample taken from a human subject who has no indication of a liver disease, wherein a primer having polynucleotide
sequences set forth in SEQ ID NO:7 or SEQ ID NO:8 is used in the RT-PCR;

(b) comparing the concentration of albumin mRNA from step (a) with a standard control;
(c) detecting the concentration from step (a) being higher than the standard control; and
(d) determining the presence of fatty liver in the subject.
US Pat. No. 9,297,798

THERAPEUTIC APPROACH FOR POLYGLUTAMINE DEGENERATION

The Chinese University of...

1. An isolated polypeptide comprising (1) a core sequence that is a fragment of nucleolin (NCL) of less than 50 amino acids
and comprises SEQ ID NO:6 and (2) a heterologous amino acid sequence located at the N-terminus or the C-terminus of the core
sequence, provided that the polypeptide does not comprise the full length NCL.

US Pat. No. 9,185,024

PATH SELECTION IN STREAMING VIDEO OVER MULTI-OVERLAY APPLICATION LAYER MULTICAST

The Chinese University of...

1. In a multicast data communication system, a method for selecting paths for video data between a source and ultimate destination
peer nodes, the method comprising:
providing in-band bandwidth probing tools at peer nodes between the source and the destination peer nodes, each said probing
tool being independently operative and without reliance on information on link capacity and numbers of flows passing through
a link as reported by routers, wherein network paths between the peer nodes are defined at an application layer of the multicast
communication system in a plurality of application layer overlays;

at each peer node that has a probing tool,
determining achievable bandwidth of video data directed through said peer node, where achievable bandwidth is established
by measured data rate, the measured data rate being determined by measuring actual data rate of incoming video data at the
peer node to determine if the measured data rate exceeds a prescribed data rate of a congestion-aware transport protocol for
the video data, wherein network paths accommodate at least one protocol other than the congestion-aware transport protocol;

using the achievable bandwidth as a metric at said peer node to determine useable bandwidth at the application layer for said
node; and

selecting a preferred path among paths defined at the application layer to divert a fraction of video data traffic from a
congested path onto another path that has the useable bandwidth.

US Pat. No. 9,920,100

MIMOTOPES OF TROPOMYOSIN FOR USE IN IMMUNOTHERAPY FOR SHELLFISH AND/OR ARTHROPOD ALLERGY

The Chinese University of...

1. An isolated peptide having at least 80% sequence identity to any one of SEQ ID NOS: 3 and 4, wherein the peptide is a mimotope
of an epitope of a tropomyosin protein.

US Pat. No. 9,406,105

BINOCULAR VISUAL EXPERIENCE ENRICHMENT SYSTEM

The Chinese University of...

1. A system for generating an image for display comprising:
a computer processor with associated digital memory and output element, said processor being operative to employ an optimization-based
binocular tone mapping framework;

said computer processor and associated memory being configured to:
receive in digital representation as a source a high-dynamic range image of a source high dynamic range image;
produce a first low-dynamic range image directly from said high-dynamic range image;
synthesize a corresponding second low-dynamic range image from said high-dynamic range image, wherein extra image space is
used to enhance image content while preserving visual information, and

present said two low-dynamic range images separately to eyes of a viewer to be processed in the brain of the viewer via human
visual perception phenomenon of binocular single vision,

said tone mapping framework being guided by a metric of a binocular viewing comfort predictor (BVCP) based upon brightness,
contrast and contour differences between two views in order to assure stable formation of binocular single vision for the
viewer and avoid viewing discomfort.

US Pat. No. 9,218,449

METHODS FOR ANALYZING MASSIVELY PARALLEL SEQUENCING DATA FOR NONINVASIVE PRENATAL DIAGNOSIS

The Chinese University of...

1. A method of characterizing a test genome or portion thereof, the method comprising:
identifying a first chromosomal region having a first GC content in a reference genome;
assembling an artificial reference chromosome including a plurality of disjoint regions of the reference genome, the plurality
of disjoint regions being at least 50 kb in length, the artificial reference chromosome having a second GC content that is
about the first GC content;

aligning, by a computer system, each of a plurality of sequence tags with the first chromosomal region and with the artificial
reference chromosome, wherein the sequence tags have been obtained by sequencing nucleic acids in a biological sample comprising
cell-free nucleic acids from a first tissue and a second tissue;

determining, by a computer system, a first amount of sequence tags that align with the first chromosomal region;
determining, by a computer system, a reference amount of sequence tags that align with the artificial reference chromosome;
determining a parameter from the first amount and the reference amount, the parameter including a ratio of the first amount
and the reference amount; and

comparing the parameter to a cutoff value, thereby determining a classification of an amplification or deletion in the first
chromosomal region of the first tissue.

US Pat. No. 9,181,535

TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES (TALENS)

THE CHINESE UNIVERSITY OF...

1. A polynucleotide sequence encoding a transcription activator like effector nuclease (TALEN), the TALEN comprising from
an N-terminus to a C-terminus:
(i) a first segment of about 50 to about 200 amino acids in length, wherein the first segment comprises the amino acid sequence
of SEQ ID NO: 1;

(ii) a TAL effector DNA-binding domain providing sequence-specific binding to a target nucleotide sequence, wherein the TAL
effector DNA-binding domain comprises 12-31 TAL repeats and a C-terminal truncated TAL repeat;

(iii) a second segment of about 20 to 100 amino acids in length, wherein the first segment comprises the amino acid sequence
of SEQ ID NO: 2; and

(iv) a modified FokI nuclease catalytic domain.
US Pat. No. 9,090,944

BIOMARKER DACT1 FOR GASTRIC CANCER

The Chinese University of...

1. A method for detecting gastric cancer in a subject, comprising the steps of:
(a) treating a gastric sample taken from the subject with a bisulfite;
(b) performing a polymerase chain reaction using a primer consisting of the polynucleotide sequence of SEQ ID NO:9, 10, 11,
12, 13, or 14 and determining methylation status of each CpG in a CpG-containing genomic sequence, wherein the CpG-containing
genomic sequence is the -465 to -406 -465 to -406 region from human DACT1 transcription start site or a fragment thereof that comprises at least one CpG; and

(c) diagnosing the subject as having gastric cancer when at least one CpG in the CpG-containing genomic sequence is at least
11% methylated.

US Pat. No. 9,478,854

DEVICES AND METHODS FOR REDUCING INTERFERENCE BETWEEN CLOSELY COLLOCATED ANTENNAS

The Chinese University of...

1. A device for reducing interference between antennas in a compact antenna array, the antenna array comprising a plurality
of antennas for transmitting signals from and receiving signals to a plurality of transceivers respectively, the device comprising:
a plurality of resonators;
a first set of ports, each of which is connected to a respective one of the plurality of transceivers; and
a second set of ports, each of which is connected to a respective one of the plurality of antennas;
wherein at least two of the plurality resonators are cascade connected between each of the first set of ports and a corresponding
one of the second set of ports;

wherein each of the transceivers and the antennas is connected to a respective one of the resonators, and coupling coefficients
among the resonators as well as a resonance frequency of each of the resonators are configured so that a desired isolation
among the first set of ports and a desired matching performance at each of the first set of ports are obtained.

US Pat. No. 10,111,722

ROBOTIC SYSTEM

THE CHINESE UNIVERSITY OF...

1. A robot for Endoscopic Submucosal Dissection (ESD), comprising:a slave device configured with a first arm and a second arm;
an endoscopic platform with at least two channels extensible to a target tissue and for receiving the first and second arms, respectively; and
a master device configured to control the first arm to retract the target tissue and control the second arm to dissect the retracted tissue;
each of the first arm and the second arm comprising a continuum structure and an end-effector connected with the continuum structure; and
the continuum structure of the first and second arms comprising at least two pairs of actuators configured to drive the end-effector, the actuators in the first arm comprising a wire and a shape memory alloy, the wire and the shape memory alloy in the actuators of the first arm being separately controlled, and being used to control the end-effector and the first arm.
US Pat. No. 9,518,298

DACH1 AS A BIOMARKER FOR DIABETES

The Chinese University of...

1. A method for assessing the presence or risk of type 2 diabetes or cardiovascular disease in a human subject, comprising
the steps of:
(a) amplifying nucleotide sequence of a portion of genomic sequence of DACH1 (Dachshund homolog 1) present in a biological
sample taken from the subject using primers consisting of SEQ ID NO:4 and SEQ ID NO:5,

(b) detecting a T allele of polymorphism rs1408888 in the portion of genomic sequence of DACH1, and
(c) determining that the subject has or is at risk of developing type 2 diabetes or cardiovascular disease.

US Pat. No. 9,190,705

DUAL MODE DIELECTRIC RESONATOR FILTER HAVING PLURAL HOLES FORMED THEREIN FOR RECEIVING TUNING AND COUPLING SCREWS

The Chinese University of...

17. A dielectric resonator filter including a plurality of dielectric resonators in a common housing, wherein the housing
includes a top surface and a bottom surface, a separating wall is provided between each of two adjacent dielectric resonators
to separate the housing into a plurality of resonator cavities, and a coupling element is provided for coupling between two
adjacent dielectric resonators, wherein each of the dielectric resonators comprises:
a dielectric rod located within the resonator cavity of the dielectric resonator, wherein the dielectric rod is short-circuited
at both the top surface and the bottom surface;

a plurality of holes are formed in the dielectric rod parallel to an axis of the dielectric rod and a plurality of apertures
are formed on the top surface corresponding to the positions of the holes, respectively; and

a plurality of screws are inserted into the holes through the apertures, respectively;
wherein each of the dielectric resonators supports dual TM11 degenerate modes, each of which forms a resonant circuit, and an insertion depth of each of the screws is adjustable to adjust
resonance frequencies of the dual degenerate modes and coupling between the dual degenerate modes, wherein a length of the
resonator cavity and a height of the resonator cavity are based on a spurious mode location and a Q value of the resonator
filter.

US Pat. No. 9,575,226

POSITIVE MICROCONTACT PRINTING

THE CHINESE UNIVERSITY OF...

1. A process for positive microcontact printing, comprising
inking a patterned mold with a thiol;
contacting the mold with a metal surface of a substrate;
backfilling the metal surface with a solution containing an aromatic amine to form a backfilling layer;
etching the metal surface covered by the thiol; and
rinsing the substrate to remove the backfilling layer.
US Pat. No. 9,556,490

METHODS FOR ASSESSING LIVER PATHOLOGIES

The Chinese University of...

1. A method for monitoring a post-liver transplant patient, comprising the steps of:
(a) determining the concentration of albumin mRNA in an acellular blood sample taken from the patient, wherein the determining
comprises amplification of the albumin mRNA by a polymerase chain reaction (PCR), and wherein a primer comprising the polynucleotide
sequence set forth in SEQ ID NO:7 or 8 is used in the PCR;

(b) comparing the concentration of albumin mRNA from step (a) with a standard control; and
(c) detecting an increased risk of post-transplant liver complication in the patient when the concentration obtained in step
(b) is greater than the standard control, and detecting no increased risk of post-transplant liver complication in the patient
when the concentration obtained in step (b) is no greater than the standard control, wherein the post-transplant liver complication
is not recurring hepatocellular carcinoma (HCC).

US Pat. No. 9,610,263

METHOD OF MODULATING MEMBRANE POTENTIAL OF A CELL

VERSITECH LIMITED, Hong ...

1. A method of modulating membrane potential of a cell membrane comprising:
forming a synthetic chloride ion channel in the cell membrane;
wherein the synthetic ion channel is formed by a plurality of molecules of a self-assembling compound having formula:

wherein D is CH(R) wherein R is:
(1) C1-6 alkyl optionally substituted with NH2;

(2) (C1-6 alkyl)C(?O)(OC1-6 alkyl);

(3) (C1-6 alkyl)S(C1-6 alkyl);

(4) (C0-6 alkyl)phenyl; or

(5) indolyl;
X is C1-20 alkyl; and

k is 1.

US Pat. No. 9,543,644

METHOD AND AN APPARATUS FOR DECOUPLING MULTIPLE ANTENNAS IN A COMPACT ANTENNA ARRAY

The Chinese University of...

15. An apparatus for decoupling two antennas in an antenna array, wherein the two antennas transmit and receive signals via
a first input/output port and a second input/output port of the apparatus, and the apparatus comprises:
a first adjusting device connected between a first antenna of the two antennas and the first input/output port;
a second adjusting device connected between a second antenna of the two antennas and the second input/output port; and
one or more decoupling networks connected between the first input/output port and the second input/output port, each of which
comprises:

a Coupled Resonator Decoupling Network (CRDN) module;
a first I/O coupling module connected between the first input/output port and the CRDN module; and
a second I/O coupling module connected between the second input/output port and the CRDN module;
wherein, the first and second I/O coupling modules have adjustable electrical parameters such that the decoupling networks
have an adjustable working frequency and an adjustable decoupling level,

wherein the first and the second adjusting devices are configured to have an electrical length and characteristic impedance,
both of which are adjustable to compensate the admittance of the decoupling networks, such that an isolation coefficient between
the two input/output ports approaches zero as well as reflection coefficients of each input/output port are minimized.

US Pat. No. 9,464,327

RECURRENT TRANSFORMING UBR5-ZNF423 FUSION GENE IN EBV-ASSOCIATED NASOPHARYNGEAL CARCINOMA

The Chinese University of...

1. A UBR5-ZNF423 fusion polypeptide comprising the amino acid sequence set forth in SEQ ID NO:3 and a heterologous tag.

US Pat. No. 9,627,751

DEVICE FOR DECOUPLING ANTENNAS IN COMPACT ANTENNA ARRAY AND ANTENNA ARRAY WITH THE DEVICE

THE CHINESE UNIVERSITY OF...

1. A device for decoupling two antennas in a compact antenna array, comprising:
a first resonator coupled with a source, the source being connected with a first antenna of the two antennas; and
a second resonator coupled with the first resonator and a load, the load being connected with a second antenna of the two
antennas,

wherein the first and second resonators are lumped element resonators that are configured so that a first coupling between
the source and the first resonator, a second coupling between the first and second resonators, and a third coupling between
the second resonator and the load are satisfied with a constraint that an isolation coefficient in a whole network composed
of a first two-port network consisting of the two antennas and a second two-port network consisting of the first and second
resonators in parallel approaches zero as well as reflection coefficients of each port of the whole network are minimized.

US Pat. No. 9,527,733

METHOD AND APPARATUS FOR DYNAMIC-TUNING

THE CHINESE UNIVERSITY OF...

1. A compliant apparatus for nano-manufacture, comprising a stage for supporting the objects to be nano-manufactured, wherein
the stage comprises at least one flexural beam and at least one actuator coupled to the flexural beam, and
wherein the actuator is configured to generate and apply axial loads onto the flexural beam in a longitudinal direction thereof,
such that a natural frequency of the flexural beam is shifted in response to the generated axial loads to allow trade-offs
between the natural frequency and a stroke of the stage for nano-manufacturing the objects, and

wherein the axial loads are generated according to a required stroke of the compliant apparatus and the natural frequency
is changed in response to the generated loads.

US Pat. No. 9,121,069

DIAGNOSING CANCER USING GENOMIC SEQUENCING

The Chinese University of...

1. A method of analyzing a biological sample of a human for deletions or amplifications in one or more chromosomal regions
associated with cancer, the biological sample including cell-free nucleic acid molecules originating from non-malignant cells
and potentially from tumor cells associated with cancer, the method comprising:
performing a random sequencing of nucleic acid molecules from biological sample by:
attaching adaptors to at least one end of the nucleic acid molecules; and
sequencing, using the adaptors, the nucleic acid molecules to obtain sequences, including at least 120,000 sequences;
receiving, at a computer system, the sequences obtained from the random sequencing of nucleic acid molecules contained in
the biological sample;

aligning, by the computer system, at least a portion of the sequences to a human genome;
determining, by the computer system, a first amount of sequences identified as aligning to a first chromosomal region that
is part of a first chromosome, wherein a deletion or an amplification in the first chromosomal region is associated with cancer;

determining, by the computer system, a second amount of sequences identified as aligning to one or more second chromosomal
regions;

determining a first parameter from the first amount and the second amount, wherein the first parameter represents a relative
amount between the first and second amounts; and

comparing the first parameter to one or more cutoff values to determine a classification of whether the first chromosomal
region exhibits a deletion or an amplification associated with cancer in the human.

US Pat. No. 9,672,412

REAL-TIME HEAD POSE TRACKING WITH ONLINE FACE TEMPLATE RECONSTRUCTION

THE CHINESE UNIVERSITY OF...

1. A computer-implemented method for tracking a head pose of a user, comprising:
retrieving a plurality of frames of images of the user;
comparing each of the retrieved frames with a predetermined face template to determine one or more head poses that are monitored
successfully and obtain head pose information of the determined one or more head poses;

selecting a plurality of frames for reconstructing the face template by:
checking, from the determined one or more head poses, a head pose is located in a preset effective fitting zone;
selecting the frame corresponding to the checked head pose as a frame to be selected; and
updating the effective fitting zone according to the checking results;
wherein the steps of checking, selecting and updating are repeated until no head pose is located in the updated effective
fitting zone, such that all frames are selected; and

reconstructing, during the step of comparing, the face template from the obtained head pose information;
wherein the reconstructed face template is compared with subsequently retrieved images such that the head poses of the user
are tracked in time.

US Pat. No. 9,701,698

SELF-ASSEMBLED MONOLAYERS OF PHOSPHONIC ACIDS AS DIELECTRIC SURFACES FOR HIGH-PERFORMANCE ORGANIC THIN FILM TRANSISTORS

THE CHINESE UNIVERSITY OF...

1. A cycloalkylalkylphosphonic acid or dialkyl cycloalkylalkylphosphonate ester, of the structure:

where n is 6-20, m is 1 to 4, and R is hydrogen, methyl, ethyl, or propyl.

US Pat. No. 9,667,382

NETWORK-CODED MULTIPLE ACCESS

The Chinese University of...

1. A two-layer decoding method in a wireless local area network (“WLAN”) comprising:
receiving a pair of overlapping packets at a network element in the WLAN transmitted concurrently from multiple users;
communicating the pair of overlapping packets to a physical layer decoding unit comprising a multi-user decoding (“MUD”) decoder
and a physical layer network coding (“PNC”) decoder, wherein the MUD decoder is adapted to attempt to decode each individual
packet of the pair of overlapping packets, and the PNC decoder is adapted to attempt to decode a network-coded packet derived
from the pair of overlapping packets transmitted from the multiple users and received at the network element;

decoding a first packet of the pair of overlapping packets using physical-layer bridging when only a second packet of the
pair of overlapping packets and the network-coded packet are decoded; and

attempting to recover each of the pair of packets based on results obtained from the physical layer decoding unit.

US Pat. No. 9,732,390

NON-INVASIVE DETERMINATION OF METHYLOME OF FETUS OR TUMOR FROM PLASMA

The Chinese University of...

1. A method of measuring a first methylation profile of a first tissue from a first biological sample of an organism, the
method comprising:
obtaining the first biological sample, the first biological sample including cell-free DNA comprising a mixture of cell-free
DNA originating from the first tissue and from a second tissue, the first tissue being fetal or placental tissue or tumor
tissue, wherein the first biological sample is selected from the group consisting of blood, plasma, serum, urine, saliva,
sputum, tears, and stool;

purifying the first biological sample for the mixture of cell-free DNA from a cellular portion of the first biological sample;
obtaining a second methylation profile corresponding to DNA of the second tissue, the second methylation profile providing
a methylation density at each of a plurality of loci in a genome, the methylation density at a particular locus corresponding
to a proportion of DNA of the second tissue that are methylated;

determining a cell-free methylation profile of the cell-free DNA of the mixture, wherein the determining the cell-free methylation
profile comprises:

performing an assay on the cell-free DNA of the mixture, wherein performing the assay on the cell-free DNA of the mixture
comprises:

sequencing the cell-free DNA, wherein the assay is performed at a genome-wide scale; and
detecting methylation statuses of the cell-free DNA from the mixture at the plurality of loci; and
determining, by a computer system, a methylation density at each of the plurality of loci using the methylation statuses of
the cell-free DNA from the mixture at the plurality of loci;

determining, by the computer system, a percentage of the cell-free DNA in the mixture that are from the first tissue; and
determining, by the computer system, the first methylation profile of the first tissue by:
for each of the plurality of loci:
calculating a differential parameter that includes a difference between the methylation density of the second methylation
profile and the methylation density of the cell-free methylation profile, the difference being scaled by the percentage.

US Pat. No. 10,090,872

METHOD AND APPARATUS FOR ESTIMATING A FREQUENCY DOMAIN REPRESENTATION OF A SIGNAL

IMPERIAL INNOVATIONS LIMI...

1. A digital signal processing method for estimating a frequency domain representation of a signal, the method comprising:obtaining, by a processor, signal sample data comprising a series of samples, wherein the samples comprise samples of a physical signal collected by a signal measurement instrument and the series of samples is distorted by an instrument function associated with the collection of the physical signal by the signal measurement instrument;
obtaining, by the processor, coefficient data comprising a set of coefficients that fit a set of basis functions to a complex exponential function wherein the set of basis functions comprises a plurality of basis functions each defined by a shifted version of the instrument function in a signal domain;
calculating, by the processor, estimates of the frequency domain representation of the signal by scaling each item of signal sample data by a corresponding item of the coefficient data and summing the scaled sample data to convert the samples of the signal into an estimate of the frequency domain representation of the signal;
wherein the estimate of the instrument function is based on a characterisation of the instrument function in the frequency domain at frequencies associated with the complex exponential function.

US Pat. No. 9,931,664

ROLL-TO-ROLL PRINTING SYSTEMS AND METHODS FOR FABRICATING PRINT ROLLER

THE CHINESE UNIVERSITY OF...

1. A roll-to-roll printing system, comprising:a print roller configured to print on a web;
an impression roller configured to impress the web onto the print roller such that the web is printed by the print roller; and
a multiple degrees of freedom control apparatus, the apparatus comprising:
a first set of activators; and
a first mechanism comprising:
two integral flexures configured to support opposite ends of the print roller, respectively, wherein the first set of activators are arranged to act on the two integral flexures, causing elastic deformation of the two integral flexures such that each of the opposite ends of the print roller supported by one of the two integral flexures independently moves in a first and a second directions perpendicular to each other, so as to control the print roller in respect of four degrees of freedom, and each of the integral flexures comprises:
a center stage for bearing one of the ends of the print roller;
a stationary frame;
an intermediate stage between the center stage and the stationary frame;
a single-piece leaf or single-beam structure for connecting the center stage with the intermediate stage of the integral flexure, wherein the single-piece leaf or single-beam structure is configured to deform so as to provide the four degrees of freedom of movement of the print roller.
US Pat. No. 9,855,248

POLYMORPH OF ITRACONAZOLE WITH IMPROVED PHARMACEUTICAL PROPERTIES

The Chinese University of...

1. A crystalline form of itraconazole characterized by an X-ray powder diffraction (XRPD) pattern comprising three or more
peaks at 11.9, 16.7, 17.4, 18.7, 19.1, 20.0 and 21.1 degrees 2? (±0.2 degrees 2?), wherein the XRPD is made using CuK?1 radiation.

US Pat. No. 9,853,358

AIR-FILLED PATCH ANTENNA

THE CHINESE UNIVERSITY OF...

1. An air-filled patch antenna, comprising:
a ground plane;
a patch arranged to be in parallel to the ground plane;
four inherent metal legs extending from the patch perpendicularly, wherein each of distal ends of the four legs is electrically
and mechanically connected to the ground plane; and

a feeding structure configured to provide a signal interface to the antenna, wherein the feeding structure comprises a power
divider located on the ground plane, two transmission lines connected to and extending from the power divider, and two n-shaped
probes each of which has an end connected to one of the two transmission lines and another end soldered to a non-grounded
soldering pad on the ground plane.

US Pat. No. 9,982,300

SIZE-BASED GENOMIC ANALYSIS

The Chinese University of...

1. A method for performing prenatal diagnosis of a sequence imbalance in a biological sample obtained from a female subject pregnant with a fetus, wherein the biological sample includes a mixture of cell-free DNA molecules that are part of DNA sequences of a human genome, the biological sample including DNA molecules from the fetus and the female subject, the method comprising:extracting the mixture of cell-free DNA to obtain the biological sample;
for each of a plurality of the DNA molecules in the biological sample:
measuring a size of the DNA molecule, wherein measuring the size of the DNA molecule comprises:
paired-end sequencing of the DNA molecule to obtain two sequence reads, wherein the paired-end sequencing is part of performing a random sequencing of the plurality of DNA molecules in the biological sample, and
identifying which nucleic acid sequence in the human genome the DNA molecule is derived from, wherein identifying which DNA sequence the DNA molecule is derived from includes:
aligning, by a computer system, the two sequence reads to the human genome;
calculating, by the computer system, a first statistical value from the sizes of DNA molecules from a first sequence;
identifying one or more reference sequences;
calculating, by the computer system, a second statistical value from the sizes of DNA molecules from the one or more reference sequences;
determining a parameter using the first statistical value and the second statistical value, the parameter being a difference or a ratio of the first statistical value and the second statistical value; and
determining a classification of whether a sequence imbalance exists for the first sequence based on a comparison of the parameter to a cutoff value.

US Pat. No. 9,984,909

SYSTEM AND METHOD FOR LASER SCRIBING A SOLAR PANEL AND THE SOLAR PANEL

The Chinese University of...

1. A method for laser-scribing a solar panel, the method comprising:capturing consecutively images of parts of a first line on the solar panel, the first line having a pre-designed shape;
acquiring location information of each of the captured images of the first line; and
laser-scribing consecutively parts of a second line on the solar panel, according to the acquired location information of the first line, so that the scribed second line has the pre-designed shape of the first line and maintains a fixed distance from the first line.
US Pat. No. 10,093,976

IDENTIFYING A DE NOVO FETAL MUTATION FROM A MATERNAL BIOLOGICAL SAMPLE

The Chinese University of...

1. A method of identifying a de novo mutation in a genome of an unborn fetus of a pregnant female, the fetus having a father and a mother being the pregnant female, the father having a paternal genome and the mother having a maternal genome, the method comprising:performing a random sequencing of a plurality of nucleic acid molecules from a biological sample obtained from the pregnant female to obtain sequence reads of the plurality of nucleic acid molecules, wherein the biological sample contains a mixture of cell-free maternal and fetal nucleic acids, and wherein the sequencing of a nucleic acid molecule is paired-end sequencing that provides a pair of sequences corresponding to both ends of the nucleic acid molecule;
receiving the sequence reads at a computer system;
identifying, by the computer system, a location of each of the plurality of nucleic acid molecules in a human genome using the sequence reads of the plurality of nucleic acid molecules, wherein identifying the location of a nucleic acid molecule in the human genome includes mapping the pair of sequences corresponding to both ends of the nucleic acid molecule to the human genome;
for each of at least a portion of the locations, determining, by the computer system, one or more maternal sequences in the maternal genome and one or more paternal sequences in the paternal genome at the location;
identifying, by the computer system, a first sequence in the sequence reads of the plurality of nucleic acid molecules at a first location that is not present in the determined maternal or paternal sequences at the first location;
determining, by the computer system, a first fractional concentration of the first sequence in the biological sample at the first location using the sequence reads corresponding to the first location;
determining, by the computer system, a second fractional concentration of a second sequence in the biological sample at a second location using the sequence reads corresponding to the second location, the second sequence being inherited by the fetus from the father at the second location, wherein the second sequence is present in the paternal genome at the second location and not present in the maternal genome at the second location;
classifying, by the computer system, the first sequence as a de novo mutation at the first location in a fetal genome of the fetus if the first and second fractional concentrations are about the same; and
when the first sequence is classified as the de novo mutation:
outputting, by the computer system, classification information identifying the first sequence as being the de novo mutation at the first location as a result of receiving the sequence reads from the sequencing of the plurality of nucleic acid molecules from the biological sample obtained from the pregnant female.
US Pat. No. 10,133,845

CLASSIFICATION OF CANCER LEVEL BASED ON GENOMIC COORDINATES OF OUTERMOST NUCLEOTIDES OF CELL-FREE DNA

The Chinese University of...

1. A method for analyzing a biological sample of a subject to generate a classification of a level of cancer in the subject, the method comprising:(a) obtaining the biological sample of the subject, the biological sample comprising a plurality of cell-free nucleic acid molecules;
(b) sequencing the plurality of cell-free nucleic acid molecules to obtain sequence reads, wherein each sequence read of the sequence reads comprises at least one outermost nucleotide at an end of a given cell-free nucleic acid molecule of the plurality of cell-free nucleic acid molecules;
(c) aligning the sequence reads to a reference genome, thereby obtaining a set of genomic coordinates including genomic coordinates of outermost nucleotides at ends of the plurality of cell-free nucleic acid molecules;
(d) calculating a value based on a number of sequence reads of the sequence reads of (b) ending at one of the set of genomic coordinates of (c); and
(e) generating the classification of the level of cancer in the subject based on processing the value against a reference value, thereby obtaining the classification using an assay that does not require a tissue-specific mutation, wherein the classification of the level of cancer corresponds to whether a cancer exists, a stage of the cancer, or a size of a tumor.

US Pat. No. 9,972,406

MODELING METHOD FOR ORTHOPEDIC CASTS

THE CHINESE UNIVERSITY OF...

1. A computer-implemented method for modeling an orthopedic cast, comprising:scanning an injured limb to extract raw body data;
determining, from the extracted raw body data, target portion data of the injured limb, the determined target portion data representing a fine cast surface for the orthopedic cast;
patterning the fine cast surface to form a blank area thereon;
forming an opening gap on the blank area for assembling and disassembling the orthopedic cast; and
offsetting the fine cast surface in parallel to thicken the orthopedic cast.

US Pat. No. 9,968,616

DISCOVERY OF FDA-APPROVED DRUGS AS INHIBITORS OF FATTY ACID BINDING PROTEIN 4 USING MOLECULAR DOCKING SCREENING

THE CHINESE UNIVERSITY OF...

1. A method of alleviating or treating a cardiovascular disease in a subject, comprising the step of administering to the subject an effective amount of a pharmaceutical composition comprising levofloxacin having the structure:

US Pat. No. 9,966,720

DIAMOND MASER AND MICROWAVE AMPLIFIER

The Chinese University of...

1. A method of providing microwave amplification, the method comprising:providing a maser system; and
providing electromagnetic radiation to the maser system, thereby causing the maser system to emit microwave radiation,
wherein the maser system comprises:
a gain medium;
a resonator disposed around the gain medium;
a cavity box disposed around the resonator; and
a magnet array disposed around the gain medium,
wherein the gain medium comprises diamond having a plurality of nitrogen-vacancy (NV) centers,
wherein the electromagnetic radiation is pumped into the cavity box of the maser system at a pump rate that is higher than the decay rate, and
wherein a cavity Q factor of the maser system is below a masing threshold of the maser system, such that the maser system operates as a microwave amplifier.

US Pat. No. 9,892,230

SIZE-BASED ANALYSIS OF FETAL OR TUMOR DNA FRACTION IN PLASMA

The Chinese University of...

1. A method of estimating a fractional concentration of fetal DNA in a biological sample from a female pregnant with a fetus,
the biological sample including the cell-free fetal DNA and other DNA, the method comprising:
measuring an amount of DNA fragments from the biological sample corresponding to each of a plurality of sizes, the amount
including the cell-free fetal DNA and the other DNA, thereby measuring amounts of DNA fragments at the plurality of sizes;

calculating, with a computer system, a first value of a first parameter based on the amounts of DNA fragments at the plurality
of sizes, the first parameter providing a statistical measure of a size profile of DNA fragments in the biological sample;

obtaining one or more first calibration data points, wherein each of the one or more first calibration data points specifies
a fractional concentration of fetal DNA corresponding to a calibration value of the first parameter, and wherein the one or
more first calibration data points are determined from a plurality of calibration samples;

comparing the first value to a calibration value of at least one of the one or more first calibration data points; and
estimating the fractional concentration of the fetal DNA in the biological sample based on the comparison, thereby obtaining
the fractional concentration using an assay that does not require a tissue-specific marker.

US Pat. No. 9,917,632

NETWORK-CODING BUILDING BLOCKS AND DECOMPOSITION SCHEDULING BASED THEREON

The Chinese University of...

1. A data communication method comprising:
identifying a plurality of physical-layer network coding (PNC) atoms in a digital telecommunications network, the digital
telecommunications network comprising a plurality of nodes and at least one relay,

each of the plurality of PNC atoms comprising:
a transmission pattern attribute,
at least some of the plurality of nodes, and
the at least one relay in the digital telecommunications network,
wherein the transmission pattern attribute of each of the plurality of PNC atoms cannot be decomposed to any other transmission
pattern attribute of the plurality of PNC atoms; and

wherein each of the plurality of PNC atoms specifies how traffic flows between the plurality of nodes and the at least one
relay using the transmission pattern attribute;

generating a transmission schedule using the plurality of PNC atoms based at least in part on the transmission pattern attribute
of at least some of the plurality of PNC atoms; and

relaying traffic between the plurality of nodes and the at least one relay, using the transmission schedule.
US Pat. No. 9,889,086

BIOADHESIVE AND INJECTABLE HYDROGEL

The Chinese University of...

1. A method of producing a hydrogel, comprising the steps of:
(1) complexing a host molecule and a guest molecule, wherein the host molecule presents a hydrophobic pocket and comprises
a monomer component, and wherein the host molecule and guest molecule form a physical interaction between the hydrophobic
pocket and a benzene ring of the guest molecule; and

(2) polymerizing the monomer components to form a cross-linked mesh of the host molecules and guest molecules.

US Pat. No. 9,965,585

DETECTION OF GENETIC OR MOLECULAR ABERRATIONS ASSOCIATED WITH CANCER

The Chinese University of...

1. A method of analyzing a biological sample of an organism, the biological sample including nucleic acid molecules originating from normal cells and potentially from cells associated with cancer, wherein at least some of the nucleic acid molecules are cell-free in the biological sample, the method comprising:identifying, by a computer system, a plurality of non-overlapping chromosomal regions of the organism, wherein each of the plurality of non-overlapping chromosomal regions includes a plurality of loci and is of predetermined length specified by the computer system before analysis of the biological sample, the predetermined length being between 100 kilobases and 50 megabases;
obtaining the biological sample of the organism, wherein the organism is not known to have a tumor;
using sequencing or sequence-specific probes on a plurality of nucleic acid molecules in the biological sample of the organism to obtain sequenced reads;
receiving the sequenced reads at the computer system;
identifying, by the computer system, a location of each of the plurality of nucleic acid molecules in a reference genome corresponding to the organism using the sequenced reads, the plurality of nucleic acid molecules including cell-free nucleic acid molecules, wherein the using sequencing or the sequence-specific probes provides sequenced reads located within the plurality of non-overlapping chromosomal regions in the reference genome;
for each of the plurality of non-overlapping chromosomal regions:
identifying a respective group of nucleic acid molecules among the plurality of nucleic acid molecules as being from the chromosomal region based on the identified locations, the respective group including at least one nucleic acid molecule located at each of the plurality of loci of the chromosomal region;
calculating, with a computer system, a respective value of the respective group of nucleic acid molecules, the respective value defining a property of the nucleic acid molecules of the respective group; and
using the respective value and a respective reference value to determine a classification of whether the chromosomal region exhibits a deletion or an amplification;
determining an amount of chromosomal regions classified as exhibiting a deletion or amplification, the amount of chromosomal regions being (i) a number of the chromosomal regions classified as exhibiting a deletion or an amplification, or (ii) a total length of the chromosomal regions classified as exhibiting a deletion or an amplification; and
analyzing the amount of chromosomal regions against a threshold value to determine a classification of whether the organism has cancer, the classification including that the organism has cancer when the amount of chromosomal regions is greater than the threshold value, the threshold value being determined based on samples having cancer and samples not having cancer.

US Pat. No. 9,897,602

MICROARRAY SUBSTRATE, MICROARRAY, MICROFLUIDIC SYSTEM AND METHODS FOR PREPARING THE SAME

THE CHINESE UNIVERSITY OF...

1. A microarray substrate, comprising a piece of fluoropolymer whose surface is modified with polydopamine, wherein the polydopamine
forms an array of microspots on the surface of the fluoropolymer piece by a microfluidic system, and wherein the microfluidic
system comprises a channel layer containing microchannels in which a solution can continuously flow, and a bottom layer which
contains an array of micropores and is under the channel layer.

US Pat. No. 10,208,806

COMPLIANT SAFE JOINT AND MANUFACTURING METHOD THEREOF

THE CHINESE UNIVERSITY OF...

1. A compliant safe joint, comprising:an input axis circumferentially connected to a motor shaft of a DC motor, wherein the motor shaft is arranged in a hole in a center of the input axis;
a movable bridge circumferentially mounted on the input axis and slidable along an axial direction of the input axis;
a plurality of bearings, each of the bearings having an inner portion fixed to the movable bridge and having an outer portion;
a stationary bridge rotatably mounted on the movable bridge and having a helicoid surface, the outer portion of each bearing being movable along the helicoid surface, wherein the helicoid surface is provided with a plurality of slots, and each of the bearings is constrained in a respective slot when the torque applied to the stationary bridge does not exceed the predetermined threshold, the helicoid surface having a predetermined slope tangent to the outer portion of the bearing when the outer portion of the bearing moves along the helicoid surface and non-tangent to the outer portion of the bearing when the outer portion of the bearing is constrained in the slot; and
a flexible component connected to the movable bridge;
wherein the stationary bridge rotates about the input axis when a torque which exceeds a predetermined threshold is applied to the stationary bridge by the motor shaft, such that the bearings move with respect to the helicoid surface to cause the flexible component to be compressed and extended through the movable bridge.

US Pat. No. 10,064,544

ENDOSCOPIC CAPSULE AND ENDOSCOPIC SYSTEM

THE CHINESE UNIVERSITY OF...

1. An endoscopic capsule comprising:a sensor for recording biological information inside a gastrointestinal tract;
an expander having an inflatable cavity, the inflatable cavity being inflatable by injecting fluid and deflatable by discharging the fluid therefrom; and
a controller determining an affected part in the gastrointestinal tract from the recorded biological information, and controlling the expander to have:
an inflation position, in which the inflatable cavity is inflated by injected fluid and the expander is, via the inflated inflatable cavity, held adjacent to the determined affected part in the gastrointestinal tract; or
a deflation position, in which at least a portion of the injected fluid in the inflatable cavity is released and the expander retracts from the gastrointestinal tract wherein the controller comprises:
a base chamber separated from the inflatable cavity and communicated with the inflatable cavity via an inflation fluid exit hole, the base chamber storing base; and
an acid chamber separated from the inflatable cavity and the base chamber, and communicated with the base chamber via a chamber connection hole, the acid chamber storing acid and comprising an end wall,
wherein in the inflation position, the end wall is pushed forward to compress the acid chamber such that the acid flows from the acid chamber into the base chamber through the chamber connection hole and is mixed with the base to generate the fluid to inflate the inflatable cavity, and
wherein in the deflation position, the end wall is pulled backward beyond a deflation fluid exit hole to let the fluid leak from the inflatable cavity.
US Pat. No. 9,862,999

MARKER FOR PRENATAL DIAGNOSIS AND MONITORING

The Chinese University of...

1. A method of analyzing the maspin gene in the blood of a pregnant woman, comprising the steps of
(a) treating DNA obtained from a blood sample taken from the woman with a reagent that differentially modifies methylated
and non-methylated DNA, thereby distinguishing methylated and unmethylated version of the maspin gene; and

(b) determining the level of at least one portion the unmethylated version of the maspin gene by performing a polymerase chain
reaction (PCR) using a primer comprising the polynucleotide sequence of SEQ ID NO:6 or 7 to amplify the at least one portion
of the maspin gene.

US Pat. No. 10,211,024

SYSTEM AND METHOD FOR AXIAL SCANNING BASED ON STATIC PHASE MASKS

The Chinese University of...

1. A method for axial-scanning a sample, comprising:generating a scanning beam along a transverse scanning direction across the sample;
acquiring radial positions of the generated scanning beam along the transverse scanning direction; and
determining, based on the radial positions of the generated scanning beam and desired focal lengths, a phase mask so that the scanning beam at different radial positions along the scanning direction is focused to different axial positions of the sample along an optical axis transverse to the scanning direction.
US Pat. No. 10,106,836

DETERMINING FETAL GENOMES FOR MULTIPLE FETUS PREGNANCIES

The Chinese University of...

1. A method of determining inheritance of maternal haplotypes in two fetuses of a female fertilized by a male, the method comprising:obtaining a blood sample from the female;
extracting plasma or serum from the blood sample to obtain a biological sample, the biological sample including cell-free DNA of the female and the two fetuses;
sequencing, with a sequencing device, DNA fragments from the plasma or serum to obtain sequence reads;
aligning, by a computer system in communication with the sequencing device, sequence reads to a reference genome to identify reads aligning to a first group of first loci on a first chromosome, wherein the female is heterozygous for a respective first allele and a respective second allele at each first locus, a first maternal haplotype having the first alleles and a second maternal haplotype having the second alleles;
determining that the two fetuses inherit the respective first alleles at the first loci from the male;
aligning, by the computer system, sequence reads to the reference genome to identify reads aligning to a second group of second loci on the first chromosome, wherein the first maternal haplotype has a respective third allele and the second maternal haplotype has a respective fourth allele at each second locus of the second group, and
determining that the two fetuses inherit the respective fourth alleles at the second loci from the male;
measuring, by the computer system, a first amount of the respective first alleles and a second amount of the respective second alleles that are present at the first loci in DNA fragments of the biological sample from the female, wherein:
measuring the first amount of respective first alleles includes counting the number of sequence reads aligning to the first loci having the respective first allele, and
measuring the second amount of respective second alleles includes counting the number of sequence reads aligning to the first loci having the respective second allele;
measuring, by the computer system, a third amount of the respective third alleles and a fourth amount of the respective fourth alleles that are present at the second loci in DNA fragment of the biological sample, wherein:
measuring the third amount of respective third alleles includes counting the number of sequence reads aligning to the second loci having the respective third allele, and
measuring the fourth amount of respective fourth alleles includes counting the number of sequence reads aligning to the second loci having the respective fourth allele; and
determining inheritance of maternal haplotypes in the two fetuses by:
(i) identifying both fetuses as having inherited the first maternal haplotype when the first amount is statistically higher than the second amount and the third amount is statistically equal to the fourth amount,
(ii) identifying both fetuses as having inherited the second maternal haplotype when the first amount is statistically equal to the second amount and the fourth amount is statistically higher than the third amount, or
(iii) identifying one of the fetuses as inheriting the first maternal haplotype and the other fetus as inheriting the second maternal haplotype when the first amount is statistically higher than the second amount and when the fourth amount is statistically higher than the third amount.
US Pat. No. 10,006,087

MARKERS FOR PRENATAL DIAGNOSIS AND MONITORING

The Chinese University of...

1. A method for detecting the presence of an RNA species in the blood of a pregnant woman, the method comprising the step of:performing a reverse transcriptase polymerase chain reaction (RT-PCR) to detect the RNA species in a plasma or serum sample obtained from the pregnant woman, wherein the RNA species is derived from RPL17, wherein an oligonucleotide primer comprising SEQ ID NO:40 or SEQ ID NO:41 is used in the RT-PCR.

US Pat. No. 10,207,365

PARALLEL LASER MANUFACTURING SYSTEM AND METHOD

THE CHINESE UNIVERSITY OF...

1. A laser-based manufacturing system, comprising:a laser source generating a pulsed laser beam;
a reflectance mirror arranged in a propagation path of the laser to reflect the laser, the mirror being controllable to adjust an emergent angle of the laser;
a deformable dispersion unit located in a laser receiving path from the reflectance mirror and having an array of micromirrors controllable in response to the adjusted emergent angle to form required laser patterns from the reflected laser, the laser patterns having spatially separated optical spectral components with a plurality of propagation angles;
one or more focusing units positioned in a propagation path of the separated optical spectral components produced by the deformable dispersion unit, the focusing units recombine the optical spectral components on or inside fabrication targets; and
a DBP System positioned between the laser source and the reflectance mirror to shape pulses of the generated pulsed laser beam, the DBP System comprising:
a beam diameter regulator located in a beam receiving path and configured to adjust a diameter of the beam received in the beam receiving path;
a first dispersion unit located in the beam receiving path and separating the spectral components from the adjusted laser to make the separated spectral components transmit out with different emergent angles;
a first focusing unit located in a beam path from the first dispersion unit and configured to collimate the spectral components of the beam with different emergent angles into a parallel laser beam and to convert the separated spectral components with different emergent angles (angular dispersion) to a plurality of spatially dispersed spectral components at a back focal plane of the first focusing unit;
a modulating unit positioned on the back focal plane to form arbitrary patterns of the laser from the spatially and spectrally dispersed optical components; and
a second focusing unit located in the beam path and is used to combine different spectral components back to a second dispersion unit positioned at a focal plane of the second focusing unit, wherein the second dispersion unit recombines all the spectral components into a single collimated beam, obtaining a re-shaped pulsed laser beam.

US Pat. No. 10,143,666

SMALL MOLECULE INHIBITORS TARGETING CAG-REPEAT RNA TOXICITY IN POLYGLUTAMINE DISEASES

The Chinese University of...

1. A method for inhibiting a polyglutamine (polyQ) disease in a subject, comprising administering to the subject an effective amount of a compound of formula I:
or a pharmaceutically acceptable salt, racemate, enantiomer, diastereomer, poly-morph, tautomer, geometric isomer, or prodrug thereof;
wherein R1, R2, R3, and R4 are each independently hydrogen, C1-8 alkyl, of substituted C1-8 alkyl; and subscripts a and b are each independently an integer from 1 to 8.

US Pat. No. 10,135,133

APPARATUS AND METHODS FOR REDUCING MUTUAL COUPLINGS IN AN ANTENNA ARRAY

The Chinese University of...

1. A near-field apparatus for reducing mutual couplings between antenna elements in an antenna array, the antenna array having a nominal operating range of frequencies and associated wavelengths, the apparatus comprising:a plurality of electrically conductive patches in a common plane configured to be supported above the antenna array, each electrically conductive patch being isolated from other patches and having a width of less than 50% of an underlying antenna element and a longest dimension no greater than 0.3 of a shortest wavelength of the nominal operating range; and
a standoff structure configured to hold the common plane of electrically conductive patches parallel to and at a height from a ground plane of the antenna array,
wherein at least one electrically conductive patch of the plurality of electrically conductive patches is sized to diffract a portion of an electromagnetic wave from an underlying antenna element to a neighboring antenna element such that a mutual coupling s-parameter between the underlying antenna element and the neighboring antenna element measured or simulated i) without the electrically conductive patches, S21Array, and ii) with the electrically conductive patches, S21ADS, with a difference defined as S21Refl=S21ADS?S21Array, meets the following criteria:
|S21Refl| is in a range of |S21Array|±20% of |S21Array|; and
Phase(S21Refl) is in a range of Phase(S21Array)+180±30 degrees.

US Pat. No. 10,167,560

METHOD AND APPARATUS FOR STRUCTURAL COLORATION OF METALLIC SURFACES

THE CHINESE UNIVERSITY OF...

1. A method of producing an iridescent metallic surface, the method comprising:defining on the metallic surface a plurality of distinct regions of the same or different shapes and sizes,
wherein a number of distinct regions corresponds to a number of colors desired for the iridescent metallic surface to display;
creating with a cutting tool on a first distinct region of the metallic surface at least one series of periodic features perpendicular to a desired cutting direction,
wherein the at least one series of periodic features are parallel or substantially parallel,
wherein the cutting tool is vibrated at a distinct frequency while simultaneously displaced along the desired cutting direction across the metallic surface, and
wherein a rate of the displacement of the cutting tool is different from the distinct frequency of the vibration of the cutting tool; and
repeating the treatment of the first distinct region of the metallic surface in each additional region of the metallic surface,
wherein the distinct frequency of the vibration of the cutting tool is held constant while the rate of the displacement of the cutting tool varies from each distinct region to each other distinct region of the metallic surface, or
wherein the rate of the displacement of the cutting tool is held constant while the distinct frequency of the vibration of the cutting tool varies from each distinct region to each other distinct region of the metallic surface.
US Pat. No. 10,152,568

NONINVASIVE PRENATAL GENOTYPING OF FETAL SEX CHROMOSOMES

The Chinese University of...

1. A method for determining whether a male fetus of a pregnant female has an X-linked mutation, wherein the pregnant female is heterozygous for a mutant and a normal allele at a locus on the X chromosome, the method comprising:performing a plurality of reactions, each involving one or more nucleic acid molecules from a biological sample, the biological sample including cell-free nucleic acid molecules from the pregnant female and from the male fetus;
detecting data from the plurality of reactions,
receiving, by a computer system, the data from the plurality of reactions, wherein the data includes:
a first set of quantitative data indicating a first amount of the mutant allele at the locus; and
a second set of quantitative data indicating a second amount of the normal allele at the locus;
determining, by the computer system, a parameter from the first amount and the second amount, wherein the parameter represents a relative amount between the first and second amounts;
obtaining, by the computer system, a percentage Pf of fetal nucleic acid molecules in the biological sample;
calculating, by the computer system, a first cutoff value for determining whether the fetus has inherited the mutant allele at the locus, wherein the first cutoff value is derived at least from a first proportion of k/(1+k?Pf), where k is a number of mutant alleles on a mutant chromosome of the pregnant female, k being an integer equal to or greater than one;
calculating, by the computer system, a second cutoff value for determining whether the fetus has inherited the normal allele at the locus, wherein the second cutoff value is derived at least from a second proportion of [k(1?Pf)]/[1+k?kPf)]; and
comparing, by the computer system, the parameter to at least one of the first and second cutoff values to determine a classification of whether the fetus has inherited the mutant allele or the normal allele.

US Pat. No. 9,941,996

MESSAGE CODING FOR NCMA-BASED MULTIPLE ACCESS NETWORKS

The Chinese University of...

1. A method for device-facilitated communication, the method comprising:receiving, at a decoding component of a device, a linear combination of a plurality of encoded data packets transmitted by a plurality of senders in accordance with a fountain code;
iteratively applying, by the decoding component, a multi-stage decoding procedure to the linear combination of the plurality of encoded data packets to provide decoded data packets corresponding to each of the plurality of senders, the multi-stage decoding procedure including:
a first stage during which a belief propagation decoding of the linear combination of the plurality of encoded data packets is performed; and
a second stage during which the linear combination of the plurality of encoded data packets is transformed to enable further applications of the first stage, wherein at least once among the iterative applications of the multi-stage decoding procedure the second stage includes a transformation corresponding to a Gaussian reduction of a matrix representing the linear combination of the plurality of encoded data packets; and
enabling communication by the senders with the decoded data packets provided by the decoding component.
US Pat. No. 10,174,375

SEQUENCING ANALYSIS OF CIRCULATING DNA TO DETECT AND MONITOR AUTOIMMUNE DISEASES

The Chinese University of...

1. A method of analyzing a biological sample of an organism, the biological sample including nucleic acid molecules, wherein at least some of the nucleic acid molecules are cell-free, the method comprising:sequencing a plurality of cell-free DNA molecules from the biological sample to obtain sequence data from both ends of each cell-free DNA molecule of the plurality of cell-free DNA molecules from the biological sample, wherein the biological sample is extracted from a bodily fluid sample from the organism;
analyzing the sequence data of the plurality of cell-free DNA molecules from the biological sample, wherein analyzing the sequence data of a cell-free DNA molecule comprises:
aligning, by a computer system, the sequence data of the cell-free DNA molecule to a reference genome,
determining, by the computer system, a size of the cell-free DNA molecule from the aligned portion of the sequence data of the cell-free DNA molecule, and
comparing the size of the cell-free DNA molecule with a threshold value;
determining, with the computer system, an amount of the cell-free DNA molecules having sizes below the threshold value;
estimating a first level of an auto-immune disease in the organism based upon the amount;
using the first level of the auto-immune disease in the organism to design a treatment regimen for the organism or determine a dose of a medication; and
providing treatment for the auto-immune disease to the organism according to the treatment regimen or with the dose of the medication.

US Pat. No. 10,167,451

COMBINATIONAL USE OF MECHANICAL MANIPULATION AND PROGRAMIN DERIVATIVES TO INCREASE OCT4, SOX2, OR NANOG EXPRESSION IN FIBROBLASTS

The Chinese University of...

1. A method for increasing Oct4, Sox2, or Nanog expression in fibroblasts, the method comprising:(a) culturing the fibroblasts in a vessel;
(b) mechanically agitating the fibroblasts that are unattached to the vessel to form a non-adherent cellular aggregate; and
(c) exposing the non-adherent cellular aggregate to an effective amount of a compound of Formula II-IX having any one of the following structures:

thereby increasing Oct4, Sox2, or Nanog expression in the fibroblasts of the non-adherent cellular aggregate.
US Pat. No. 10,208,348

DETERMINING PERCENTAGE OF FETAL DNA IN MATERNAL SAMPLE

The Chinese University of...

1. A method of measuring a fractional concentration of fetal DNA in a biological sample of a female subject pregnant with a fetus, the biological sample including a mixture of cell-free nucleic acid molecules from the female subject and from the fetus, and wherein the female subject is homozygous at a first locus for a first allele and the fetus is heterozygous at the first locus for the first allele and a second allele different from the first allele, the method comprising:separating the cell-free nucleic acid molecules of the biological sample into a first plurality of reactions;
measuring signals from the first plurality of reactions, each of the first plurality of reactions involving the first locus, wherein the signals are indicative of a presence or absence of the first allele and the second allele at the first locus in each of the first plurality of reactions;
receiving, at a computer system, first data including the measured signals from the first plurality of reactions involving cell-free nucleic acid molecules from the biological sample, wherein the first data includes:
(1) a first set of quantitative data indicating a first number of reactions positive for the first allele; and
(2) a second set of quantitative data indicating a second number of reactions positive for the second allele; and
comparing, by the computer system, the first number to the second number to measure the fractional concentration of fetal DNA.

US Pat. No. 10,176,819

PHONETIC POSTERIORGRAMS FOR MANY-TO-ONE VOICE CONVERSION

The Chinese University of...

1. A computer-implemented method comprising:obtaining a target speech;
obtaining a source speech;
generating a target phonetic posteriorgram (PPG) of the target speech by driving a first model with acoustic features of the target speech, the target PPG including a set of values corresponding to a range of times and a range of phonetic classes;
extracting target mel-cepstral coefficients (MCEP) features from the target speech;
training a second model using the target MCEP features and the target PPG to obtain a mapping between the target MCEP features and the target PPG;
generating a source PPG of the source speech by driving the first model with acoustic features of the source speech; and
converting the source speech into a converted speech using the source PPG and the trained second model.

US Pat. No. 10,164,330

ANTENNA ASSEMBLY AND SELF-CURING DECOUPLING METHOD FOR REDUCING MUTUAL COUPLING OF COUPLED ANTENNAS

The Chinese University of...

1. An antenna assembly, comprising:a first antenna having a shorting arm;
a first tapping stub projecting from the shorting arm of the first antenna;
a second antenna having a shorting arm;
a second tapping stub projecting from the shorting arm of the second antenna; and
a ground plane connecting the first antenna and the second antenna;
wherein a first lumped capacitor is connected between the first tapping stub and the ground plane, and a second lumped capacitor is connected between the second tapping stub and the ground plane, so that a mutual coupling between the first antenna and the second antenna is reduced.

US Pat. No. 10,197,793

LIGHT MODULATOR USING TOTAL INTERNAL REFLECTION AT AN INTERFACE WITH A TUNABLE CONDUCTIVE LAYER

The Chinese University of...

1. A modulator for a light beam, the modulator comprising:a prism having a first surface to receive a light beam, a second surface to provide total internal reflection of the light beam, and a third surface to permit the light beam to exit the prism;
a tunable conductive layer disposed on the second surface of the prism such that a reflectivity of the second surface is a function of an electrical conductivity of the tunable conductive layer; and
a control mechanism to dynamically control the electrical conductivity of the tunable conductive layer and thereby control the reflectivity of the second surface of the prism.
US Pat. No. 10,329,606

METHODS AND KITS FOR SELECTIVELY AMPLIFYING, DETECTING OR QUANTIFYING TARGET DNA WITH SPECIFIC END SEQUENCES

The Chinese University of...

1. A method for detecting and/or quantifying a hypomethylated target DNA fragment in a sample containing a corresponding hypermethylated target DNA fragment, wherein both the hypomethylated target DNA fragment and the corresponding hypermethylated target DNA fragment contain a methylation-sensitive restriction enzyme recognition site, the method comprising:(a) digesting the sample with a methylation-sensitive restriction enzyme which recognizes the methylation-sensitive restriction enzyme recognition site;
(b) contacting the digested sample with a stem-loop primer, wherein a 3? portion of the stem-loop primer is complementary to the 3? end of a strand of a digested DNA fragment at the methylation-sensitive restriction enzyme recognition site, and a 5? portion of the stem-loop primer is capable of forming a stem-loop structure;
(c) denaturing the mixture of step (b) to denature the digested DNA fragment and stem-loop primer;
(d) annealing the stem-loop primer to the strand of the restriction enzyme-digested DNA fragment via the 3? portion thereof and performing an extension reaction by using the strand of the digested DNA fragment as a template, wherein the stem-loop primer does not efficiently anneal to the hypermethylated target DNA fragment which is not cut or digested; and
(e) detecting and/or quantifying the extension product with a PCR assay, wherein one primer of the primer pair used in the PCR assay is specific to the stem-loop primer,
wherein results of the PCR assay indicate the presence and/or quantity of the hypomethylated target DNA fragment in the sample.

US Pat. No. 10,609,747

SYSTEM AND METHOD FOR PEER-TO-PEER WIRELESS COMMUNICATION

The Chinese University of...

1. A method, comprising:transmitting, by a first device to a server, a request to initiate an offline interaction with a second device, the offline interaction not hosted by the server, the request including credential information of a first party associated with the first device;
receiving, by the first device from the server, a pairing code and a first handshake code, the pairing code for authenticating the second device and the first handshake code for authorizing a second party associated with the second device to engage in the offline interaction with the first party, the pairing code and the first handshake code being provided to the first device after the server authenticates the first party using the credential information;
transmitting, by the first device, at least a first portion of the pairing code in a first broadcast message that is capable of being detected by the second device;
receiving, by the first device from the second device, a second broadcast message;
authenticating, by the first device, the second device based on at least a second portion of the pairing code in the second broadcast message;
responsive to authenticating the second device, establishing, by the first device, a wireless peer-to-peer communication channel with the second device;
receiving, by the first device from the second device, a second handshake code via the wireless peer-to-peer communication channel;
authorizing, by the first device, the second party to engage in the offline interaction with the first party based on a relationship between the first handshake code and the second handshake code; and
responsive to authorizing the second party, performing, by the first device, one or more actions to facilitate the offline interaction.

US Pat. No. 10,189,862

PHENOTHIAZINE-PYRIDINE COMPOUNDS AND USES THEREOF

The Chinese University of...

15. A pharmaceutical composition comprising the compound of claim 1 and a pharmaceutically acceptable excipient.

US Pat. No. 10,457,990

ACCURATE DEDUCTION OF FETAL DNA FRACTION WITH SHALLOW-DEPTH SEQUENCING OF MATERNAL PLASMA

The Chinese University of...

1. A method of measuring a fetal DNA fraction in a biological sample of a female pregnant with a fetus, the biological sample including maternal DNA molecules and fetal DNA molecules, the method comprising performing, by a computer system:identifying a plurality of sites based on sequence information that indicates the female is homozygous for a first allele at each site of the plurality of sites;
obtaining a plurality of reads from DNA molecules of the biological sample;
identifying locations of the plurality of reads in a reference genome;
determining a first value of a first group of DNA molecules based on the plurality of reads, wherein:
each DNA molecule of the first group of DNA molecules includes a read located at a site of the plurality of sites and exhibits a second allele different from the first allele at the site,
the first value defines a property of the first group of DNA molecules, and
a portion of the sites of the plurality of sites do not comprise a read exhibiting a second allele different from the first allele;
determining a second value of a second group of DNA molecules, wherein:
each DNA molecule of the second group of DNA molecules includes a read located at a site of the plurality of sites and exhibits the first allele at the site, and
the second value defines the property of the second group of DNA molecules;
determining a parameter value of a parameter from the first value and the second value;
comparing the parameter value to a calibration point that is determined using at least one other sample having a known fetal DNA fraction and a calibration value corresponding to separate measurement of the parameter in the at least one other sample; and
computing the fetal DNA fraction based on the comparison.

US Pat. No. 10,444,199

EVANESCENT-WAVE QUARTZ-ENHANCED PHOTOACOUSTIC SENSOR WITH RESONATOR ELEMENTS

THE CHINESE UNIVERSITY OF...

1. An evanescent-wave quartz enhanced microfiber photoacoustic detection device with oscillator and micro-resonator elements for detecting trace gas concentrations, the evanescent-wave quartz enhanced microfiber photoacoustic detection device comprising:a light source tuned to a wavelength corresponding to the optical absorption of a gas to be detected;
an optical fiber;
a fiber-taper to generate an evanescent wave, a coating layer of the fiber-taper being stripped;
a quartz tuning fork having its free arms arranged at the level of the fiber-taper to absorb a mechanical force generated following the optical absorption by the gas, the mechanical force
exciting a piezoelectric mode of the quartz tuning fork and generating an electrical current;
a micro-resonator disposed adjacent to the quartz tuning fork and configured to enhance the mechanical force;
a transimpedance amplifier amplifying a current generated by the quartz tuning fork to determine the concentration of the gas; and
a lock-in amplifier directly connected to the transimpedance amplifier,
wherein the fiber-taper, quartz tuning fork, and micro-resonator are assembled in a sealed gas cell for gas detection.
US Pat. No. 10,240,209

DETECTING MUTATIONS FOR CANCER SCREENING

The Chinese University of...

1. A method for identifying somatic mutations in a human subject by analyzing a biological sample of the human subject, the biological sample including DNA fragments originating from normal cells and potentially from tumor cells or cells associated with cancer, the biological sample including cell-free DNA fragments, the method comprising:obtaining template cell-free DNA fragments from the biological sample to be analyzed;
preparing a sequencing library of analyzable cell-free DNA molecules using the template cell-free DNA fragments, the preparation of the sequencing library of analyzable cell-free DNA molecules not including a step of DNA amplification of the template cell-free DNA fragments;
sequencing the sequencing library of analyzable cell-free DNA molecules to obtain a plurality of sequence reads of cell-free DNA molecules;
receiving, at a computer system, the plurality of sequence reads;
aligning, by the computer system, the plurality of sequence reads to a reference human genome to determine genomic positions for the plurality of sequence reads;
obtaining, by the computer system, information about a constitutional genome corresponding to the human subject; and
comparing, by the computer system, the plurality of sequence reads of cell-free DNA molecules to the constitutional genome to identify a filtered set of loci as having somatic mutations in some tissue of the human subject, wherein:
at each locus of the filtered set, a number of the plurality of sequence reads of cell-free DNA molecules having a sequence variant relative to the constitutional genome is above a cutoff value, the cutoff value being greater than one.

US Pat. No. 10,208,104

FAST AND EFFICIENT CONJUGATION METHOD BASED ON THIOUREA-CATECHOL COUPLING

The Chinese University of...

1. A method of preparing a multi-compound chemical structure using a catechol-functionalized compound and a thiourea-functionalized di-compound polymer, comprising the steps of:(a) oxidizing the catechol-functionalized compound in the presence of an oxidizing agent to form the corresponding oxidized catechol-functionalized compound; and
(b) conjugating the oxidized catechol-functionalized compound with the thiourea-functionalized di-compound polymer to form a catechol-conjugated tri-compound polymer.

US Pat. No. 10,598,751

SYSTEM AND METHOD FOR SEPARATION OF WATER AND FAT SIGNALS DURING SPIN-LOCK MAGNETIC RESONANCE IMAGING

THE CHINESE UNIVERSITY OF...

1. A method of generating an image of a region of interest of a subject using a magnetic resonance imaging (MRI) apparatus, the method comprising:applying a magnetization prep sequence that includes an adiabatic continuous-wave constant-amplitude spin-lock (ACCSL) pulse sequence;
performing an acquisition sequence to acquire a data set wherein the acquisition sequence incorporates a Dixon acquisition method;
generating a source image from the data set; and
analyzing the source image using a Dixon analysis method to generate a water image and a fat image.

US Pat. No. 10,568,502

VISUAL DISABILITY DETECTION SYSTEM USING VIRTUAL REALITY

The Chinese University of...

1. A visual disability detection method implemented by computer, or a processor executing non-transitory computer readable storage medium comprising code, the method including the steps of:generating a virtual reality simulation in a virtual reality environment with virtual reality objects, wherein the virtual reality simulation simulates a real life activity that tests visual responses of the user;
displaying the virtual reality simulation on a head-mounted display;
instructing the user to perform a task with a video and audio interface;
adjusting of a brightness level of the virtual reality simulation and a contrast level of the virtual reality environment being displayed on the head-mounted display to simulate different lighting conditions;
monitoring voluntary and involuntary responses of the user via a sensor system during the virtual reality simulation;
computing performance scores based on the voluntary and the involuntary responses of the user to the virtual reality simulation, wherein the performance scores are computed over the different lighting conditions; and
determining visual disability metrics of the user based on the performance scores.

US Pat. No. 10,533,981

AIR QUALITY MEASUREMENT WITH MODULAR SENSOR SYSTEM AND METHOD

The Chinese University of...

1. An apparatus for acquiring air quality data from one or more removable or replaceable sensors, processing and storing the acquired air quality data, and transmitting the processed and stored air quality data to a backend server, the apparatus comprising:a circuit board;
a microcontroller electrically coupled with the circuit board;
a removable or replaceable sensor; and
a first standardized sensor interface electrically coupled with the circuit board and the microcontroller, the first standardized sensor interface comprising:
a first physical electronic coupler configured to transmit a continuous electrical power to the removable or replaceable sensor;
a second physical electronic coupler configured to transmit an intermittent electrical power to the removable or replaceable sensor during time intervals corresponding to sensing intervals determined by the microcontroller;
a third physical electronic coupler configured to transmit an indication to the microcontroller that the removable or replaceable sensor is currently electrically coupled with the first sensor interface;
a fourth physical electrical coupler configured to provide an electrical ground utilizable by electronics of the removable or replaceable sensor; and
one or more additional physical electronic couplers configured as a serial peripheral interface,
wherein the removable or replaceable sensor comprises physical electronic couplers that correspond to the physical electronic couplers of the first standardized sensor interface.

US Pat. No. 10,364,467

USING SIZE AND NUMBER ABERRATIONS IN PLASMA DNA FOR DETECTING CANCER

The Chinese University of...

1. A method of analyzing a biological sample of an organism, the biological sample including nucleic acid molecules originating from normal cells and potentially from cells associated with cancer, wherein at least some of the nucleic acid molecules are cell-free in the biological sample, the method comprising:identifying a plurality of chromosomal regions of the organism, each chromosomal region including a plurality of loci;
for each of a plurality of the nucleic acid molecules in the biological sample:
measuring a size of the nucleic acid molecule; and
identifying a location of the nucleic acid molecule in a reference genome of the organism;
for each of the plurality of chromosomal regions:
identifying a respective group of nucleic acid molecules as being from the chromosomal region based on the identified locations, the respective group including at least one nucleic acid molecule located at each of the plurality of loci of the chromosomal region;
calculating, with a computer system, a respective amount of the respective group of nucleic acid molecules; and
comparing the respective amount to a count reference value to determine a count classification of whether the chromosomal region exhibits an aberration, the count classification for a first chromosomal region of the plurality of chromosomal regions indicating a first aberration;
identifying a first group of nucleic acid molecules as being from the first chromosomal region based on the identified locations;
calculating, with a computer system, a first statistical value of a first size distribution of the first group of nucleic acid molecules;
comparing the first statistical value to a size reference value to determine a size classification of whether the first chromosomal region exhibits the first aberration;
determining a final classification of whether the first chromosomal region exhibits the first aberration using the count classification and the size classification of the first chromosomal region; and
determining whether cancer exists in the organism using the final classification.
US Pat. No. 10,297,342

CLASSIFICATION OF CANCER LEVEL BASED ON GENOMIC COORDINATES OF OUTERMOST NUCLEOTIDES OF CELL-FREE DNA

The Chinese University of...

1. A method for analyzing a biological sample of a subject to generate a classification of a level of cancer in the subject, the method comprising:(a) obtaining the biological sample of the subject, the biological sample comprising a plurality of cell-free nucleic acid molecules;
(b) sequencing the plurality of cell-free nucleic acid molecules to obtain sequence reads, wherein each sequence read of the sequence reads comprises at least one outermost nucleotide at an end of a given cell-free nucleic acid molecule of the plurality of cell-free nucleic acid molecules;
(c) aligning the sequence reads to a reference genome, thereby obtaining a set of genomic coordinates including genomic coordinates of outermost nucleotides at ends of the plurality of cell-free nucleic acid molecules;
(d) calculating a value based on a number of sequence reads of the sequence reads of (b) ending at one of the set of genomic coordinates of (c); and
(e) generating the classification of the level of cancer in the subject based on processing the value against a reference value, thereby obtaining the classification using an assay that does not require a tissue-specific mutation, wherein the classification of the level of cancer corresponds to whether a cancer exists, a stage of the cancer, or a size of a tumor.

US Pat. No. 10,240,199

MATERNAL PLASMA TRANSCRIPTOME ANALYSIS BY MASSIVELY PARALLEL RNA SEQUENCING

The Chinese University of...

1. A method of diagnosing a pregnancy-associated disorder using a sample from a female subject pregnant with a fetus, the method comprising:applying an assay to RNA molecules obtained from the sample to generate a plurality of reads that include different alleles at one or more loci for measuring relative expression levels between the different alleles in the sample, wherein the assay does not target a reference gene for normalizing expression levels;
receiving the plurality of reads, wherein the reads are obtained from an analysis of RNA molecules obtained from the sample, the sample containing a mixture of maternal- and fetal-derived RNA molecules;
identifying, by a computer system, locations of the reads in a reference sequence;
identifying one or more informative loci, each of which is homozygous in a first entity for a corresponding first allele and which is heterozygous in a second entity for the corresponding first allele and a corresponding second allele, wherein the first entity is the pregnant female subject or the fetus, and the second entity is the other one of the pregnant female subject and the fetus;
filtering the one or more informative loci to identify one or more filtered informative loci:
that are located within an expressed region of the reference sequence,
at which at least a first predetermined number of reads in the plurality of reads containing the corresponding first allele are located, and
at which at least a second predetermined number of reads in the plurality of reads containing the corresponding second allele are located,
for each of the one or more filtered informative loci:
determining a first number of reads located at the filtered informative locus and containing the corresponding first allele, and
determining a second number of reads located at the filtered informative locus and containing the corresponding second allele,
calculating a first sum of the first numbers and the second sum of the second numbers;
calculating a ratio of the first sum and the second sum, and
comparing the ratio to a cutoff value to determine whether the fetus, mother, or pregnancy has a pregnancy-associated disorder, the cutoff value being determined from one or more samples obtained from pregnant female subjects without the pregnancy-associated disorder, thereby determining whether the fetus, mother, or pregnancy has a pregnancy-associated disorder without normalizing with respect to a reference gene or a total amount of RNA molecules in the sample.

US Pat. No. 10,237,782

HARDWARE ACCELERATION FOR BATCHED SPARSE CODES

The Chinese University of...

16. A method for device-facilitated communication, the method comprising:receiving, with a pipelined multiplier component, input data packets and coefficients of a matrix associated with a random linear network code;
multiplying, with the pipelined multiplier component, bytes of the input data packets with corresponding coefficients in a pipelined manner;
receiving, with an addition component, an output of the pipelined multiplier component and feedback data;
adding, with the addition component, the output of the pipelined multiplier component to a byte of the feedback data;
routing, with a first switch, output of the addition component to a first memory until the first memory stores valid output data based at least in part on the output of the addition component; and
routing, with a second switch, valid output data from the first memory along a feedback data path to the addition component such that the valid output data serves as feedback data utilized to generate new valid output data in a second memory.

US Pat. No. 10,680,155

METHODS OF FABRICATION OF FLEXIBLE MICRO-THERMOELECTRIC GENERATORS

The Chinese University of...

1. A method of fabricating a flexible micro-thermoelectric generator (TEG), the method comprising:forming a first flexible polymer layer supported on a substantially rigid substrate;
forming a flexible TEG by electroplating, at least in part, by:
forming a plurality of conductive metal electrodes on the polymer layer supported on the substrate so as to define a plurality of bottom interconnectors;
patterning a mold over the plurality of conductive metal electrodes, the mold having a plurality of designed holes corresponding to a plurality of thermoelectric pillars of the TEG that include a plurality of p-type and n-type thermoelectric pillars;
depositing thermoelectric materials on top of the plurality of metal electrodes confined by the plurality of designed holes in the mold;
forming a plurality of conductive metal electrodes over the mold so as to define top interconnectors connecting all thermoelectric pillars of the plurality electrically in series; and
removing the flexible TEG from the substantially rigid substrate.

US Pat. No. 10,557,906

QUANTITATIVE MAGNETIC RESONANCE IMAGING RELAXOMETRY WITH SUPPRESSION OF BLOOD SIGNAL

The Chinese University of...

1. A method of generating an image using a magnetic resonance imaging (MRI) apparatus, the method comprising:applying a magnetization reset pulse sequence;
applying a magnetization preparation pulse sequence for quantification of a relaxation parameter, wherein a time between applying the magnetization reset pulse sequence and applying the magnetization preparation pulse sequence corresponds to a T1 recovery period;
applying an acquisition pulse sequence including a fast spin echo (FSE) pulse sequence, the FSE pulse sequence including an excitation pulse and a plurality of refocusing pulses, wherein a delay time between applying the magnetization preparation pulse sequence and applying the acquisition pulse sequence is 2 ms or less;
acquiring data during the acquisition pulse sequence;
generating image data based on the acquired data, wherein in the image data, a blood signal is at least partially suppressed; and
quantifying, based on the image data, the relaxation parameter at each of a plurality of locations within a region of a subject of interest.

US Pat. No. 10,364,471

TUMOR SUPPRESSOR REC8 AS A BIOMARKER FOR GASTRIC CANCER

The Chinese University of...

1. A method for detecting the methylation status of REC8 gene, comprising the steps of:(1) treating genomic DNA obtained from a biological sample taken from a subject with a bisulfate;
(2) performing an amplification reaction to amplify the treated genomic DNA from step (1) using a primer comprising the nucleotide sequence of SEQ ID NO:11 or 12;
(3) analyzing the product of the amplification reaction from step (2) to determine the methylation status of REC8 gene.

US Pat. No. 10,357,369

METHOD FOR PRODUCING KNEE REPLACEMENT IMPLANT AND IMPLANT FOR KNEE REPLACEMENT

Shandong Weigao Orthopaed...

1. A method of producing a knee replacement implant, the method comprising:defining a hip center, a knee center, an ankle center and a posterior femoral condylar axis of a lower limb;
generating femoral geometric parameters and tibial geometric parameters, the femoral geometric parameters and the tibial geometric parameters being measured in relation to the hip center, the knee center, the ankle center and the posterior femoral condylar axis;
collecting anthropometric data from a defined population using the femoral geometric parameters and the tibial geometric parameters; and
creating a femoral component and/or a tibial component using the collected anthropometric data,
wherein the generating of the femoral geometric parameters and the tibial geometric parameters comprises:
building a plurality of different coordinate systems based on the hip center, the knee center, the ankle center and the posterior femoral condylar axis; and
defining the femoral geometric parameters and the tibial geometric parameters by using the different coordinate systems, respectively; and
wherein the defining of the posterior femoral condylar axis comprises:
marking a surface area from an inter-notch arc to an end of a posterior femur condyle of a femur condyle of the lower limb;
fitting a cylinder with the marked surface area, in which the cylinder extends along a direction from a medial femoral condyle to a lateral femoral condyle; and
defining an axis of the fitted cylinder as the posterior femoral condylar axis.
US Pat. No. 10,301,365

MET E 1 TROPOMYOSIN VARIANTS FOR USE IN ALLERGEN-SPECIFIC IMMUNOTHERAPY

The Chinese University of...

1. An isolated nucleic acid comprising a polynucleotide sequence encoding the polypeptide comprising the amino acid sequence set forth in SEQ ID NO:3 or 4.
US Pat. No. 10,294,530

BIOMARKER FOXF2 FOR GASTRIC CANCER

The Chinese University of...

1. A method for identifying a human subject with an increased risk for gastric cancer, comprising the steps of:(a) determining the number of methylated CpGs in SEQ ID NO: 9 by treating genomic DNA from a gastric sample taken from a human subject with a bisulfite and amplifying the bisulfite treated genomic DNA with a primer pair comprising a primer comprising SEQ ID NO: 7 or SEQ ID NO: 8; and
(b) identifying the human subject as having an increased risk for gastric cancer when the number of methylated CpGs in SEQ ID NO: 9 is greater than the number of methylated CpGs in SEQ ID NO: 9 in a control non-cancerous gastric sample.
US Pat. No. 10,273,543

METHODS FOR ASSESSING LIVER PATHOLOGIES

The Chinese University of...

1. A method for identifying a presence or absence of liver cirrhosis or hepatitis, comprising:a) obtaining an acellular sample from a human subject that is asymptomatic with respect to liver cirrhosis or hepatitis, which acellular sample comprises albumin mRNA;
b) generating albumin cDNA by reverse transcribing the albumin mRNA and performing a polymerase chain reaction (PCR), wherein a primer comprising the polynucleotide sequence set forth in SEQ ID NO:7 or 8 is used in the PCR;
c) determining an amount of the albumin cDNA;
d) determining an amount of the albumin mRNA in the acellular sample based on the amount of albumin cDNA;
e) detecting (i) an increase or decrease in the amount of the albumin mRNA, or (ii) substantially no change in the amount of the albumin mRNA, which increase, decrease or substantially no change in the amount is with respect to a standard control; and
f) identifying a presence of liver cirrhosis or hepatitis in the human subject if an increase or decrease in the amount is detected in (e), or identifying an absence of liver cirrhosis or hepatitis in the human subject if substantially no change in the amount is detected in (e).
US Pat. No. 10,240,190

NANO-CONSTRUCTS FOR POLYNUCLEOTIDE DELIVERY

The Chinese University of...

1. A nano-construct comprising:(1) a core particle comprising gold and having a diameter of 40-50 nm;
(2) a polymer coating comprising polydopamine on the surface of the core particle having a thickness of 4 to 5 nm; and
(3) a polynucleotide non-covalently attached to the polymer coating,
wherein the polynucleotide comprises a core segment of 15-30 nucleotides in length, a first pairing segment located at the 5? of the core segment, and a second pairing segment located at the 3? of the core segment, wherein the polynucleotide is further attached to a fluorescent molecule fluorescein isothiocyanate (FITC) or cyanine 3 (Cy3), wherein:
when the core segment is not hybridized to its complementary sequence, the first and second pairing segments hybridize with each other so as to allow the polynucleotide to assume a hair-pin configuration and fluorescence from the fluorescent molecule to be quenched, and
when the core segment is hybridized to its complementary sequence, the first and second pairing segment separate from each other so as to allow the polynucleotide to assume an open configuration and fluorescence from the fluorescent molecule to be detectable.

US Pat. No. 10,772,141

SYSTEM AND METHOD FOR PEER-TO-PEER WIRELESS COMMUNICATION

The Chinese University of...

1. A method, comprising:transmitting, by a first device to a server, a request to initiate an offline interaction with a second device, the offline interaction not hosted by the server, the request including credential information of a first party associated with the first device;
receiving, by the first device from the server, a pairing code and a first handshake code, the pairing code for authenticating the second device and the first handshake code for authorizing a second party associated with the second device to engage in the offline interaction with the first party, the pairing code and the first handshake code being provided to the first device after the server authenticates the first party using the credential information;
transmitting, by the first device, at least a first portion of the pairing code in a first broadcast message that is capable of being detected by the second device;
receiving, by the first device from the second device, a second broadcast message;
authenticating, by the first device, the second device based on at least a second portion of the pairing code in the second broadcast message;
responsive to authenticating the second device, establishing, by the first device, a wireless peer-to-peer communication channel with the second device;
receiving, by the first device from the second device, a second handshake code via the wireless peer-to-peer communication channel;
authorizing, by the first device, the second party to engage in the offline interaction with the first party based on a relationship between the first handshake code and the second handshake code; and
responsive to authorizing the second party, performing, by the first device, one or more actions to facilitate the offline interaction.

US Pat. No. 10,626,944

MAGNETO-RHEOLOGICAL SERIES ELASTIC ACTUATOR

The Chinese University of...

1. An actuator, comprising:a first torsion spring body; and
a second torsion spring body,
each of the first torsion spring body and the second torsion spring body comprising:
an inner ring;
an outer ring; and
a plurality of elastic units, connected in parallel between the inner ring and the outer ring;
the outer ring of the first torsion spring body and the outer ring of the second torsion spring body being rigidly fastened, and the inner ring of the first torsion spring body and the inner ring of the second torsion spring body being aligned,
a motor element for providing an output torque, and connected with the inner ring of the first torsion spring body; and
a braking element for providing a braking torque, and connected with the inner ring of the second torsion spring body.

US Pat. No. 10,603,688

MICROTOME

The Chinese University of...

1. A microtome comprising:a blade cutting a soft material in a first direction, the first direction being a feeding direction of the soft material;
a blade holder holding the blade;
an actuator providing a vibration in a second direction along a cutting edge of the blade; and
a frequency-tunable resonator driven by the actuator into vibration and fixedly connected to the blade holder to transfer the vibration to the blade holder and the blade, the resonator having a tunable resonant frequency in the second direction;
wherein the frequency-tunable resonator comprises:
two pair of beams extending in the first direction and having an adjustable effective length in the first direction, wherein each of the two pair of beams is in a plate shape, and is flexible in the second direction and stiff in the first direction and a third direction perpendicular to the first and second directions.

US Pat. No. 10,594,037

DOUBLE TORSION COIL MAGNETIC CURRENT ANTENNA FEEDING STRUCTURE

The Chinese University of...

1. An antenna apparatus having a magnetic-current feeding structure, the apparatus comprising:a ground plane;
a top face at a fixed height from the ground plane and configured for radiating at an operating wavelength ?;
a conductive wire helix having an axis parallel with the ground plane, the helix located at a height below the top face and above the ground plane, the helix having one half coiled in a right-handed direction and another half coiled in a left-handed direction, the halves connected with each other at a middle point of the helix; and
an excitation stub connected with an end of the conductive wire helix, the excitation stub configured for connecting with an excitation source.
US Pat. No. 10,570,177

P3 PEPTIDYLIC INHIBITORS FOR TREATING CAG-REPEAT RNA TOXICITY IN POLYGLUTAMINE DISEASES

The Chinese University of...

1. An isolated polypeptide consisting of the amino acid sequence of SEQ ID NO:1, wherein the C-terminus is amidated, and wherein the N-terminus is acetylated or lipidated.

US Pat. No. 10,542,894

METHOD FOR MEASURING CARDIOVASCULAR AND RESPIRATORY PARAMETERS BASED ON MULTI-WAVELENGTH PHOTOPLETHYSMOGRAPHY

The Chinese University of...

1. A method for deriving a blood volume pulsation signal within a certain depth of a predetermined body part, the method comprising:determining a range of target measuring depth or layer of tissue in the predetermined body part;
selecting wavelength combinations of photoplethysmography (PPG) signals to derive a PPG signal specific for the range of target depth or layer of tissue;
emitting light of two or more different wavelengths to the predetermined body part and measuring multi-wavelength PPG signals; and
extracting the derived depth-specific PPG signal from the multi-wavelength PPG signals, wherein the derived depth-specific PPG signal is proportional to the blood volume within the range of target depth or layer of tissue in the predetermined body part,
wherein deriving the depth-specific PPG signal includes removing blood volume pulsation profiles in a superficial layer detected by a shallow penetrating light from the blood volume pulsation profiles in a deep layer detected by a deep penetrating light, and
wherein removing blood volume pulsation profiles detected by the shallow penetrating light comprises:
building a multiple-tissue layer model based on the measured multi-wavelength PPG signals and the derived depth-specific PPG signal by incorporating parameters comprising light wavelengths, light absorption coefficients, and light penetration depths;
determining the light absorption ratios of different light wavelengths; and
deriving the depth-specific PPG signal that reflects the blood volume pulsation in a specific tissue depth from multi-wavelength PPG signals by substituting the light absorption ratios that reflect the difference of the tissue absorption characteristics of the different light wavelengths.
US Pat. No. 10,533,189

HIGHLY SPECIFIC DELIVERY OF POLYNUCLEOTIDES TO THE CELL NUCLEUS VIA COMPRESSION

THE CHINESE UNIVERSITY OF...

1. A method for delivering a polynucleotide into a cell and/or a nucleus of a cell, the method comprising:a) contacting the polynucleotide with the cell,
b) applying pressure of about 0.1 pascal (Pa) to about 50 Pa on the polynucleotide and the cell by a sheet of a solid material such that the polynucleotide is forced into the cell and/or the nucleus of the cell,
wherein the polynucleotide comprises a stretch of thymidine nucleotides (polyTtail) at least 5 nucleotides long or a stretch of uridine or deoxy-uridine (polyUtail) at least 5 nucleotides long at the 3? or 5? end.
US Pat. No. 10,435,754

DIAGNOSTIC METHOD

The Chinese University Of...

1. A method for detection, prognostication, or monitoring of nasopharyngeal cancer using a biological sample from an individual, the method comprising:(a) obtaining DNA from the biological sample, wherein the biological sample is selected from the group consisting of blood, plasma, serum, saliva, and urine;
(b) digesting the DNA with one or more methylation-sensitive restriction enzymes, wherein the one or more methylation-sensitive restriction enzymes preferentially cleave DNA sequences when present in an unmethylated state than in a methylated state;
(c) determining a level of target sequence in the DNA digested in (b), wherein the target sequence comprises promoter sequence of RASSF1A and at least two methylation-sensitive restriction enzyme recognition sites; and
(d) detecting, prognosticating, or monitoring nasopharyngeal cancer based on the level of the target sequence determined in (c).

US Pat. No. 10,406,000

ANKLE-FOOT PROSTHESIS DEVICE

The Chinese University of...

1. A powered ankle-foot prosthetic device for restoring a required ankle torque for a gait to an amputee wearing the prosthetic device, the prosthetic device comprising:an ankle joint that defines an ankle angle between a shin bracket and a bracket support foot;
a motor operably coupled to the ankle joint, wherein the motor is configured to:
selectively provide a motor torque output to achieve the required ankle torque during phases of the gait, wherein selectively providing the motor torque output comprises:
providing the motor torque output during an initial phase of a controlled dorsiflexion phase;
providing the motor torque output during swing phase;
and
providing the motor output torque in a powered plantar flexion phase of a stance phase; and
a parallel spring mechanism including a cam operably coupled with the ankle joint in parallel with the motor, wherein the parallel spring mechanism is configured to:
provide a spring torque output during the controlled dorsiflexion phase and
wherein the prosthetic device is configured such that the spring torque output and the motor torque output provide the required ankle torque that produces a curved torque versus ankle angle plot during the controlled dorsiflexion phase.

US Pat. No. 10,392,666

NON-INVASIVE DETERMINATION OF METHYLOME OF TUMOR FROM PLASMA

The Chinese University of...

1. A method of analyzing a biological sample of an organism, the biological sample including DNA molecules originating from normal cells and potentially from cells associated with cancer, wherein at least some of the DNA molecules are cell-free in the biological sample, the method comprising:performing a methylation-aware assay on each of a plurality of the cell-free DNA molecules from the biological sample, wherein the methylation-aware assay comprises massively parallel sequencing;
analyzing the plurality of cell-free DNA molecules from the biological sample, wherein analyzing each of the plurality of cell-free DNA molecules includes:
determining a location of the cell-free DNA molecule in a genome of the organism using the methylation-aware assay; and
determining whether the cell-free DNA molecule is methylated at one or more sites on the cell-free DNA molecule using the methylation-aware assay, the one or more sites of each of the plurality of cell-free DNA molecules providing a plurality of sites;
for each of the plurality of sites:
individually counting, by a computer system, a respective number of the plurality of cell-free DNA molecules that are methylated at the site;
calculating, by the computer system, a first methylation level using the respective number of cell-free DNA molecules methylated at each of the plurality of sites;
comparing the first methylation level to a first cutoff value; and
determining a first classification of a level of cancer based on the comparison.
US Pat. No. 10,379,124

TUMOR SUPPRESSOR VSTM2A AS A BIOMARKER FOR COLORECTAL CANCER

The Chinese University of...

1. A method for assessing risk for colorectal cancer in a human subject, comprising the steps of:(a) treating genomic DNA from a colorectal sample taken from the subject with a bisulfate;
(b) performing a polymerase chain reaction (PCR) using a primer comprising nucleotide sequence of SEQ ID NO:15 or 16 to determine number of methylated CpGs in a genomic sequence, which is the 167-319 segment of SEQ ID NO:17 or a fragment thereof comprising at least 5 CpGs, and
(c) comparing the number of methylated CpGs from step (b) with the number of methylated CpGs in the same genomic sequence, which is taken from a human non-cancer colorectal sample and has been processed through steps (a) and (b); and
(d) determining the human subject, whose colorectal sample contains more methylated CpGs in the genomic sequence determined in step (b) compared to the number of methylated CpGs with the number of methylated CpGs in the same genomic sequence from a human non-cancer colorectal sample and having been processed through steps (a) and (b), as having an increased risk for colorectal cancer compared with a healthy human subject not diagnosed with colorectal cancer.

US Pat. No. 10,319,463

COMBINED SIZE- AND COUNT-BASED ANALYSIS OF MATERNAL PLASMA FOR DETECTION OF FETAL SUBCHROMOSOMAL ABERRATIONS

The Chinese University of...

1. A method of identifying a subchromosomal aberration in a fetal genome of a fetus by analyzing a biological sample from a female subject pregnant with the fetus, the biological sample including cell-free DNA molecules from the female subject and the fetus, the method comprising:for each of a plurality of DNA molecules in the biological sample:
measuring a size of the DNA molecule;
identifying a location of the DNA molecule in a reference genome, thereby obtaining locations of the plurality of DNA molecules;
detecting, by a computer system, an aberration in the biological sample of a first subchromosomal region by:
determining a first amount of the plurality of DNA molecules located in the first subchromosomal region using the locations of the plurality of DNA molecules;
determining a second amount of the plurality of DNA molecules located in a second region using the locations of the plurality of DNA molecules;
computing a count parameter from the first amount and the second amount; and
comparing the count parameter to one or more count threshold to determine a count classification of a type of aberration existing in the biological sample for the first subchromosomal region;
determining, by the computer system, a size classification for the first subchromosomal region by:
calculating a first statistical value of sizes of the plurality of DNA molecules located in the first subchromosomal region;
calculating a reference statistical value of sizes of DNA molecules located in a reference region;
determining a separation value between the first statistical value and the reference statistical value; and
comparing the separation value to one or more size thresholds to obtain the size classification; and
determining whether the fetus has the aberration in the first subchromosomal region based on the size classification and the count classification.
US Pat. No. 10,689,702

BIOMARKERS FOR DIABETES

The Chinese University of...

1. A method for reducing risk of type 2 diabetes in a subject, comprising the steps of:(a) performing a polymerase chain reaction (PCR) to determine nucleotide sequence of at least a portion of genomic sequence of carboxypeptidase E (CPE) present in a biological sample taken from the subject,
(b) detecting a G allele in polymorphism rs1583645,
(c) determining the subject as having an increased risk of developing type 2 diabetes based on a G allele being detected in polymorphism rs1583645, and
(d) administering to the subject who is determined in step (c) as having an increased risk of developing type 2 diabetes a medicine to reduce risk of onset of diabetes.
US Pat. No. 10,689,706

METHYLATION PATTERN ANALYSIS OF HAPLOTYPES IN TISSUES IN A DNA MIXTURE

The Chinese University of...

1. A method of determining a portion of a fetal genome of an unborn fetus of a pregnant female using a biological sample from the pregnant female, wherein the biological sample including a mixture of cell-free DNA molecules from a plurality of tissues types, including maternal tissue types and a fetal tissue type, the unborn fetus having a father and a mother being the pregnant female, the method comprising:analyzing, by a computer system, a plurality of cell-free DNA molecules from the biological sample, the plurality of cell-free DNA molecules being at least 1,000 cell-free DNA molecules, wherein analyzing a cell-free DNA molecule includes:
identifying a location of the cell-free DNA molecule in a reference human genome; and
determining a respective allele of the cell-free DNA molecule;
determining a first haplotype and a second haplotype of a first chromosomal region of a first parental genome of a first parent of the unborn fetus;
identifying one or more heterozygous loci of the first chromosomal region of the first parental genome, each heterozygous locus including a corresponding first allele in the first haplotype and a corresponding second allele in the second haplotype;
identifying a first set of the plurality of cell-free DNA molecules that each:
is located at any one of the one or more heterozygous loci,
includes the corresponding first allele of the heterozygous locus, and
includes at least one of N genomic sites, N being an integer greater than or equal to 2;
measuring N first mixture methylation levels at the N genomic sites using the first set of the plurality of cell-free DNA molecules;
determining, by the computer system, a first fractional contribution of the fetal tissue type in the mixture using the N first mixture methylation levels;
identifying a second set of the plurality of cell-free DNA molecules that each:
is located at any one of the one or more heterozygous loci,
includes the corresponding second allele, and
includes at least one of the N genomic sites;
measuring N second mixture methylation levels at the N genomic sites using the second set of the plurality of cell-free DNA molecules;
determining, by the computer system, a second fractional contribution of the fetal tissue type in the mixture using the N second mixture methylation levels;
computing a first separation value between the first fractional contribution and the second fractional contribution; and
determining the portion of the fetal genome at the one or more heterozygous loci based on the first separation value.
US Pat. No. 10,633,713

DIAGNOSTIC APPLICATIONS USING NUCLEIC ACID FRAGMENTS

The Chinese University of...

1. A method of analyzing a biological sample, including a mixture of cell-free nucleic acid molecules, to determine a level of pathology in a subject from which the biological sample is obtained, the mixture including nucleic acid molecules from the subject and potentially nucleic acid molecules from a virus, the method comprising:for each of a plurality of nucleic acid molecules in the biological sample:
measuring a size of the nucleic acid molecule, wherein the plurality of nucleic acid molecules includes at least 10,000 nucleic acid molecules;
determining whether the nucleic acid molecule is from a reference genome, thereby determining a set of nucleic acid molecules from the reference genome, the reference genome corresponding to the virus;
determining a statistical value of a size distribution of the set of nucleic acid molecules from the reference genome, the size distribution spanning a range of different sizes; and
determining the level of pathology in the subject by processing the statistical value against a cutoff value, wherein the statistical value being above the cutoff value indicates a different level of pathology than the statistical value being below the cutoff value.

US Pat. No. 10,604,808

MARKER FOR PRENATAL DIAGNOSIS AND MONITORING

The Chinese University of...

1. A method of analyzing the maspin gene in the blood of a pregnant woman, comprising the steps of(a) treating DNA obtained from a blood sample taken from the woman with a reagent that differentially modifies methylated and non-methylated DNA, thereby distinguishing methylated and unmethylated version of the maspin gene; and
(b) performing an amplification reaction to amplify at least one portion of the maspin gene.

US Pat. No. 10,499,870

METHODS AND APPARATUSES FOR QUANTIFYING VASCULAR FLUID MOTIONS FROM DSA

The Chinese University of...

1. A method for quantifying vascular fluid motions from digital subtraction angiography (DSA) images, comprising:calculating an optical flow field between two temporal consecutive DSA images; and
estimating a displacement of blood or tissue between the two temporal consecutive DSA images from the calculated optical flow field,
wherein the optical flow field is calculated by solving a minimization problem of a combined local global (CLG) energy function, wherein the CLG energy function combines the temporally extended variant of Horn-Schunck approach with Lucas-Kanade approach non-linearly in spatiotemporal approach,
wherein the CLG energy function is established by:
establishing an energy function by Horn-Schunck approach, which includes an energy term and a regularization term, wherein the energy term is determined by an estimated optical flow increment field and a gradient of an intensity field, and the intensity field is extended from a spatial field to a spatial-temporal field according to the two temporal consecutive DSA images; and
transforming the gradient of the intensity field in a spatial-temporal vector space to a localized spatial-temporal derivative smoothing tensor by a convolution kernel, wherein the localized spatial-temporal derivative smoothing tensor is defined as J?(?3I)=K?*(?3I?3IT), wherein ? denotes for a localized constant flow assumed in Lucas-Kanade approach, K? denotes for a Gaussian kernel with standard deviation ?, I denotes for the intensity field of DSA images, ?3I=[Ix, Iy, It]T, denoting for the gradient of the intensity field of DSA images, wherein
x and y denote for a two-dimensional spatial position, t denotes for a time.
US Pat. No. 10,501,797

NONINVASIVE PRENATAL DIAGNOSIS OF FETAL TRISOMY BY ALLELIC RATIO ANALYSIS USING TARGETED MASSIVELY PARALLEL SEQUENCING

The Chinese University of...

1. A method of analyzing a biological sample from a female subject pregnant with a fetus to determine whether the fetus has an aneuploidy associated with a first chromosome, the biological sample containing a mixture of cell-free DNA molecules from the fetus and the female subject, the method comprising:enriching the biological sample for DNA molecules from a plurality of target regions that are known to have polymorphic loci;
after the enriching, sequencing the DNA molecules from the biological sample to obtain a plurality of sequence reads;
analyzing, by a computer system, the plurality of sequence reads, wherein analyzing a sequence read includes:
identifying a location of the sequence read in a reference genome by aligning the sequence read to the reference genome; and
determining a respective allele of the sequence read;
identifying, by the computer system, a plurality of first loci on the first chromosome, the plurality of first loci corresponding to a portion of the target regions;
identifying, by the computer system, a plurality of second loci on one or more reference chromosomes, the plurality of second loci corresponding to a portion of the target regions, wherein the pregnant female is homozygous for a respective maternal allele at each of the first and second loci, and wherein the fetus is heterozygous for the respective maternal allele and a respective paternal allele at each of the first and second loci, the respective paternal alleles being different from the respective maternal alleles;
determining, by the computer system, a first maternal amount of the respective maternal alleles at the plurality of first loci;
determining, by the computer system, a first paternal amount of the respective paternal alleles at the plurality of first loci;
calculating a first ratio of the first maternal amount and the first paternal amount;
determining, by the computer system, a second maternal amount of the respective maternal alleles at the plurality of second loci;
determining, by the computer system, a second paternal amount of the respective paternal alleles at the plurality of second loci;
calculating a second ratio of the second maternal amount and the second paternal amount;
calculating a third ratio of the first ratio and the second ratio; and
comparing the third ratio to one or more cutoff values to determine whether the fetus has an aneuploidy associated with the first chromosome, wherein a first cutoff value of the one or more cutoff values corresponds to an aneuploidy that is paternally-derived.

US Pat. No. 10,502,578

METHODS AND SYSTEMS FOR EFFICIENT AND TIMELY TRANSPORTATION OF HEAVY-DUTY TRUCKS

The Chinese University of...

1. A method for determining a route for a truck, the method comprising:receiving, at a computer system, input information indicating a starting location, a destination location, a deadline, truck information, and weight;
defining, at a computer system, a road network model, the road network model including a plurality of nodes and a plurality of edges connecting pairs of the nodes, wherein each edge corresponds to a road and has a generalized cost function associated therewith, the generalized cost function depending in part on a speed-dependent fuel consumption model associated with the truck information and in part on a speed-dependent delay time associated with the edge, wherein the generalized cost function for each edge is determined based at least in part on a fuel-rate-speed function derived from a characteristic of the corresponding road and the speed-dependent fuel consumption model associated with the truck information;
identifying, by the computer system, a source node of the road network model that corresponds to the starting location and a destination node of the road network model that corresponds to the destination location;
performing, by the computer system, a binary search in a dual problem space to find an optimal path and speed though the road network model from the source node to the destination node, wherein each path and speed has a cost determined from the generalized cost functions of the edges included in the path and wherein the optimal path and speed has a lowest cost subject to a constraint based on arriving at the destination node by the deadline;
identifying, by the computer system, an optimal route and speed for the truck based on the binary search; and
providing, by the computer system, the optimal route and speed to a user.
US Pat. No. 10,428,383

BIOMARKERS FOR PREMATURE BIRTH AND USE THEREOF

The Chinese University of...

1. A method for prophylactic treatment of premature birth, comprising the steps of:(a) measuring mRNA level of marker gene B3GNT5 in a blood sample taken from a pregnant woman by a reverse transcriptase polymerase chain reaction (RT-PCR), wherein the B3GNT5 mRNA level is normalized over the mRNA level of reference gene GAPDH in the same sample;
(b) detecting the B3GNT5 mRNA level obtained in step (a) to be higher than the standard control and determining the woman as having increased risk of premature birth; and
(c) providing prophylactic treatment for premature birth to the woman determined in step (b) as having increased risk of premature birth, wherein the prophylactic treatment comprises administration of an antenatal corticosteroid, a tocolytic drug, or transdermal glyceryl trinitrate.
US Pat. No. 10,421,968

AMPHIPHILIC DENDRIMERS COMPLEXED WITH SIRNA FOR TREATMENT OF CANCER

Versitech Limited, Hong ...

1. A pharmaceutical composition comprising an amphiphilic dendrimer complexed with a Bcl3 siRNA having the sequence of SEQ ID NO: 1.

US Pat. No. 10,425,192

LOSS-RESILIENT PROTOCOLS FOR COMMUNICATION NETWORKS

The Chinese University of...

1. A method for device-facilitated communication, the method comprising:receiving, at a recoding node of a communication network, data packets associated with a batch of data packets encoded in accordance with a random linear network coding scheme based at least in part on a generating matrix having a first rank;
determining, by the recoding node, a second rank of correctly received linearly independent data packets of the batch, the second rank being different from the first rank;
recoding, by the recoding node, the batch of data packets at least in part by (i) generating a number of explicitly new random linear combinations of the received data packets as recoded data packets, the generated number of recoded data packets based at least in part on an arithmetic difference between the first rank and the second rank and (ii) providing data packets corresponding to the correctly received linearly independent data packets of the batch as remaining data packets in the recoded batch; and
providing the recoded batch of data packets for propagation to a decoding node.

US Pat. No. 10,401,603

HIGH-SPEED BINARY LASER BEAM SHAPING AND SCANNING

THE CHINESE UNIVERSITY OF...

1. A device for shaping and scanning a pulsed laser beam containing a different frequency spectrum, comprising:a digital micromirror device (DMD) consisting of a plurality of micromirrors, configured to receive the laser beam and shape the received laser beam with a binary hologram, wherein the DMD simultaneously functions as a programmable binary mask and a blazed grating;
a dispersion compensation unit, arranged before or after the DMD, including a blazed grating configured to disperse a spectrum of the laser beam so as to transfer the laser beam from the laser source to the DMD with a designated angular dispersion for neutralizing the angular dispersion introduced by the DMD; and
a lens pair comprising a first lens L1 and a second lens L2, configured to collimate and expand the pulsed laser beam and image the beam onto the DMD, wherein the second lens L2 has a focal length fL2 and is arranged between the DMD and the blazed grating, wherein a distance SG-L2 between the blazed grating and the lens L2 and a distance SL2-D between the lens L2 and the DMD satisfy the following equation set:

wherein m is an integer indicating a diffraction order, d is a grating period, ?i and ?m are incident and diffraction angles of a mth order diffraction, respectively, and G and D are the blazed grating and the DMD, respectively.

US Pat. No. 10,338,526

DEVICES AND METHODS FOR HOLOGRAPHIC 3D IMAGING

THE CHINESE UNIVERSITY OF...

1. A device, comprising:a laser light source that delivers a laser beam;
an image sensor;
an aperture disc comprising at least two stationary pinholes, wherein the laser beam is filtered by the stationary pinholes in a direction from the laser light source to the image sensor to generate a reference wave and an object wave;
a sample having a first area containing an object to be imaged and a second area without any object, wherein the first area is illuminated by the object wave and the second area is illuminated by the reference wave; and
wherein the image sensor captures an off-axis hologram for reconstructing an image of the object, wherein the reference wave and the object wave are interfered on the image sensor and the hologram is captured based on an interference pattern of the reference wave and the object wave.

US Pat. No. 10,220,388

CENTRIFUGAL MICROFLUIDIC CONTROL SYSTEMS AND METHOD FOR CONFIGURING THE SAME

The Chinese University of...

1. A centrifugal microfluidics control system, comprising:a centrifugal tube;
a centrifugal unit accommodating the centrifugal tube and providing a centrifugal force to the centrifugal tube;
a control unit fixed in the bottom of the centrifugal tube; and
a microfluidic supporting unit coupled to the control unit in the centrifugal tube, the microfluidic supporting unit and the control unit being arranged inside the tube,
wherein the control unit changes an orientation of the microfluidic supporting unit to change a direction of the centrifugal force applied to the microfluidic supporting unit.
US Pat. No. 10,741,270

SIZE-BASED ANALYSIS OF CELL-FREE TUMOR DNA FOR CLASSIFYING LEVEL OF CANCER

The Chinese University of...

1. A method of analyzing a biological sample of an organism, the biological sample comprising cell-free DNA, the method comprising:for each size of a plurality of sizes, measuring an amount of DNA fragments from a first set of DNA fragments from the biological sample, the amount corresponding to one of the plurality of sizes, the amount including the cell-free DNA, thereby measuring amounts of DNA fragments at the plurality of sizes, the first set of DNA fragments corresponding to a plurality of regions of a genome of the organism;
calculating a first value of a parameter based on the amounts of DNA fragments at the plurality of sizes;
comparing the first value to a reference value; and
determining a classification of a level of cancer in the organism based on the comparison.

US Pat. No. 10,707,130

SYSTEMS AND METHODS FOR DICING SAMPLES USING A BESSEL BEAM MATRIX

The Chinese University of...

1. A system for dicing a sample by a Bessel beam matrix, comprising:a Bessel beam matrix generator for generating a Bessel beam matrix including multiple Bessel beams arranged in a matrix form, according to a predetermined dicing layout of the sample;
a controller for controlling a focus position of each Bessel beam in the generated Bessel beam matrix; and
a focuser for focusing simultaneously the Bessel beams of the Bessel beam matrix at the respective controlled focus positions within the sample for dicing,
wherein the focuser is further configured to:
shift one or more of the Bessel beams in the Bessel beam matrix along a column direction and a row direction through the sample to dice the sample; and
shift independently one or more of the Bessel beams in the Bessel beam matrix along an axial direction through the sample to dice the sample.
US Pat. No. 10,683,557

DETECTING BACTERIAL TAXA FOR PREDICTING ADVERSE PREGNANCY OUTCOMES

The Chinese University of...

1. A method for reducing the risk of preterm birth, said method comprising:(a) identifying a pregnant female human subject suitable to receive cerclage/pessary intervention by the process of i. detecting in a cervical swab sample or a cervical mucus sample taken from a pregnant female human subject who has been diagnosed with cervical insufficiency the level of each of bacteria taxa Parvimonas micra, Ureaplasma urealyticum or Ureaplasma parvum, Atopobium vaginae, Peptoniphilus lacrimalis, Megasphaera cerevisiae, and Parvibacter caecicola, wherein the level is determined by massively parallel sequencing (MPS) with Cumulative Sum Scaling (CSS) normalization; ii. detecting a log10(total of all bacteria taxa levels obtained in step i) no greater than 1.15; and iii. identifying the subject as suitable to receive cerclage/pessary intervention,
and
(b) treating the subject identified in step (a) by cerclage/pessary intervention, thereby reducing the risk of preterm birth in the subject.

US Pat. No. 10,679,740

SYSTEM AND METHOD FOR PATIENT PRIVACY PROTECTION IN MEDICAL IMAGES

THE CHINESE UNIVERSITY OF...

1. A method of de-identifying a medical image, the method comprising:obtaining medical image data representing anatomy of a patient, the medical image data including a set of voxels defined in a three-dimensional space, each voxel having an original intensity value;
analyzing the medical image data to generate an image mask that assigns each of the voxels to either a foreground region or a background region such that a skin surface at a boundary between the foreground region and the background region corresponds to one or more surface anatomical features of the patient;
modifying the image mask by moving a randomly selected subset of voxels from the foreground region to the background region such that the skin surface is reshaped; and
modifying the medical image data by assigning a uniform background intensity value to each voxel of the medical image data that is assigned to the background region of the modified image mask while preserving the original intensity values of each voxel of the medical image data that is assigned to the foreground region of the modified image mask.

US Pat. No. 10,676,720

COMBINATIONAL USE OF MECHANICAL MANIPULATION AND PROGRAMIN DERIVATIVES TO INCREASE OCT4, SOX2, NANOG OR C-MYC EXPRESSION IN FIBROBLASTS

The Chinese University of...

1. A method for producing a cellular aggregate from fibroblasts, wherein the cellular aggregate comprise fibroblasts with an increase in expression of at least one of Oct4, Sox2, Nanog, and c-Myc, the method comprising:(a) culturing the fibroblasts at a density of about 1×105 to about 1×106 cells/ml in a nonadhesive vessel;
(b) mechanically agitating the fibroblasts in the culture to form the cellular aggregate, wherein the formation of the cellular aggregate induces expression of at least one of Oct4, Sox2, Nanog, and c-Myc in the fibroblasts
(c) exposing the cellular aggregate to an effective amount of a compound having any one of the following structures,

such that the fibroblasts in the cellular aggregate have an increase in expression of at least one of Oct4, Sox2, Nanog and c-Myc.
US Pat. No. 10,457,993

BIOMARKER RNF6 FOR COLORECTAL CANCER

The Chinese University of...

1. A method for assessing risk for colon cancer, comprising the steps of:(a) performing a polymerase chain reaction (PCR) to determine RNF6 level in a colon tissue sample taken from a human subject, wherein the RNF6 level is genomic RNF6 level or RNF6 mRNA level, and wherein a primer comprising the nucleotide sequence of SEQ ID NO:1 or 2 is used in the PCR;
(b) comparing the RNF6 level obtained in step (a) with a standard control level obtained from a non-cancer colon tissue sample; and
(c) determining the subject, who has an increased RNF6 level compared with the standard control level, as having an increased risk for colon cancer.

US Pat. No. 10,384,209

MICROFLUIDIC PLATFORM AND METHOD FOR CONTROLLING THE SAME

THE CHINESE UNIVERSITY OF...

12. A method for moving and heating a fluidic sample, the method comprising:receiving a fluidic sample in a planar spiral path, the spiral path emanating from a center in a spiral curve winding around the center at a continuously increasing distance from the center, the spiral path having a first section, a second section, and a constriction between the first section and the second section;
rotating the fluidic sample at a rotational speed lower than a threshold rotational speed so that the fluidic sample is stopped by the constriction and remains in the first section;
heating the fluidic sample to a first temperature;
rotating the fluidic sample at the rotational speed higher than the threshold rotational speed so that the fluidic sample squeezes through the constriction to enter the second section; and
heating the fluidic sample to a second temperature in the second section.

US Pat. No. 10,759,729

COMPOUNDS FOR TREATING DIABETES

The Chinese University of...

1. An aryl pseudo-C-glycoside compound having the structure of:
US Pat. No. 10,731,217

MARKERS FOR PRENATAL DIAGNOSIS AND MONITORING

The Chinese University of...

1. A method for detecting the presence of an RNA species in the blood of a pregnant woman, the method comprising the step of:performing a reverse transcriptase polymerase chain reaction (RT-PCR) to detect the RNA species in a plasma or serum sample obtained from the pregnant woman, wherein the RNA species is RPL17.

US Pat. No. 10,731,224

ENHANCEMENT OF CANCER SCREENING USING CELL-FREE VIRAL NUCLEIC ACIDS

The Chinese University of...

1. A method of analyzing a biological sample of a subject that is a human, the biological sample including a mixture of cell-free DNA molecules from a genome of the subject and from one or more other genomes, the method comprising:analyzing a plurality of cell-free DNA molecules from the biological sample, the plurality of cell-free DNA molecules being 1,000 or more, wherein analyzing a group of the plurality of cell-free DNA molecules includes:
identifying locations of the cell-free DNA molecules in a particular viral genome of a cancer-causing virus; and
determining whether the cell-free DNA molecules are methylated at one or more sites of the particular viral genome based on the locations;
measuring one or more mixture methylation levels based on one or more amounts of the plurality of cell-free DNA molecules methylated at a set of one or more sites of the particular viral genome;
comparing the one or more mixture methylation levels to one or more reference methylation levels determined from at least two cohorts of other subjects, wherein the at least two cohorts have different classifications associated with the particular viral genome, the different classifications including a first condition, and wherein the first condition is a cancer; and
determining a first classification of whether the subject has the first condition based on the comparing.

US Pat. No. 10,722,745

INTERACTIVE CYCLING SYSTEM AND METHOD OF USING MUSCLE SIGNALS TO CONTROL CYCLING PATTERN STIMULATION INTENSITY

The Chinese University of...

1. A system for using muscle signals to control a cycling pattern, the system comprising:a pedal;
a crank arm connected to the pedal;
a motor connected to the crank arm;
a stimulator connected to a plurality of stimulation electrodes;
a controller connected to the motor and the stimulator; and
a data acquisition system connected to the controller and a muscle activity sensor;
wherein the motor is configured to apply either an assistive force or a resistive force to the crank arm,
wherein the stimulator is configured to connect to the plurality of stimulation electrodes and transmit a stimulation signal,
wherein the data acquisition system is configured to receive a real-time muscle signal,
wherein the controller is configured to direct the motor to apply either the assistive force or the resistive force based upon the real-time muscle signal, and
wherein the controller is configured to direct the stimulator to increase or decrease an intensity of the stimulation signal based upon the real-time muscle signal.
US Pat. No. 10,724,101

BIOMARKER SCNN1B FOR GASTRIC CANCER

The Chinese University of...

1. A method for measuring expression level of SCNN1B protein in a subject, comprising the step of:(a) selecting a subject who has an increased risk for gastric cancer; and
(b) contacting a stomach mucosa sample taken from the subject with an antibody that specifically binds to a SCNN1B protein having the amino acid sequence of SEQ ID NO:12, thereby determining the expression level of SCNN1B protein in the stomach mucosa sample.

US Pat. No. 10,706,957

NON-INVASIVE DETERMINATION OF METHYLOME OF TUMOR FROM PLASMA

The Chinese University of...

1. A method of analyzing a biological sample of an organism, the biological sample comprising cell-free DNA originating from normal cells and potentially from cells associated with cancer, the method comprising:analyzing a plurality of cell-free DNA molecules from the biological sample, wherein analyzing each of the plurality of cell-free DNA molecules includes:
determining a location of the cell-free DNA molecule in a genome of the organism; and
determining whether the cell-free DNA molecule is methylated at one or more sites, wherein determining whether the cell-free DNA molecule is methylated at the one or more sites comprises performing methylation-aware sequencing, and wherein performing methylation-aware sequencing comprises performing methylation-aware massively parallel sequencing, the one or more sites of each of the plurality of cell-free DNA molecules providing a plurality of sites;
for each site of the plurality of sites, individually counting a respective number of the cell-free DNA molecules at the site that are hypermethylated; and
calculating a first methylation level using the respective numbers of cell-free DNA molecules hypermethylated at the plurality of sites.
US Pat. No. 10,643,738

NONINVASIVE PRENATAL MOLECULAR KARYOTYPING FROM MATERNAL PLASMA

The Chinese University of...

1. A method of identifying microamplifications or microdeletions in a genome of a fetus by analyzing a biological sample obtained from a female subject pregnant with the fetus, the biological sample including cell-free DNA from the fetus and from the female subject, the method comprising:obtaining, by massively parallel sequencing, one or more sequence tags for each of a plurality of DNA fragments in the biological sample;
receiving the one or more sequence tags;
determining genomic positions for the sequence tags, wherein determining genomic positions for the sequence tags includes aligning the sequence tags to a reference genome;
for each of a plurality of genomic regions:
determining, with a computer system, a respective amount of DNA fragments within the genomic region from sequence tags having genomic positions within the genomic region;
normalizing the respective amount to obtain a respective density; and
comparing the respective density to a reference density to identify whether the respective density is statistically different from the reference density;
determining whether any of the genomic regions identified to have a respective region identified to have a respective density statistically different from the reference density, wherein each of the plurality of genomic regions is 10 Mb or less;
when at least N first genomic regions identified to have respective densities statistically higher than the reference density are consecutive, identifying the consecutive first genomic regions as a microamplification, N being an integer equal to or greater than three;
when at least N second genomic regions identified to have respective densities statistically lower than the reference density are consecutive, identifying the consecutive second genomic regions as a microdeletion; and
determining whether the microamplification or the microdeletion is maternally inherited, wherein a microamplification or microdeletion is determined to be maternally inherited if, for each of the consecutive genomic regions corresponding to the microamplification or microdeletion, the difference between the respective density and reference density exceeds a particular cutoff, the particular cutoff being larger than a cutoff used to determine whether the respective density and reference density are statistically different.
US Pat. No. 10,619,214

DETECTING GENETIC ABERRATIONS ASSOCIATED WITH CANCER USING GENOMIC SEQUENCING

The Chinese University of...

1. A method of analyzing a biological sample obtained from a subject being screened for a genetic aberration in a chromosomal region associated with cancer, said biological sample comprising cell-free nucleic acid molecules, said method comprising:(a) performing sequencing of said cell-free nucleic acid molecules from said biological sample of said subject to generate sequence reads;
(b) receiving, at a computer system, said sequence reads obtained from said sequencing of said cell-free nucleic acid molecules from said biological sample;
(c) aligning at least a portion of said sequence reads to a reference genome;
(d) determining a parameter of a relative amount between (i) a first amount of said sequence reads that align to a first chromosomal region that is part of a first chromosome in the reference genome, and (ii) a second amount of said sequence reads that align to one or more second chromosomal regions in the reference genome; and
(e) determining, using said parameter, whether said first chromosomal region comprises a genetic aberration associated with cancer in cell-free nucleic acid molecules of said biological sample that are derived from a tumor.

US Pat. No. 10,525,173

HYBRID IMPLANT SYSTEM AND MANUFACTURING METHOD THEREFOR

The Chinese University of...

1. A hybrid implant system for fixing and repairing orthopedic fracture, comprising:an implant body, having holes on both ends; and
a locking part, configured for attaching the implant body to a broken bone through the holes;
a healing assembly made from a material promoting healing of the bone, the implant body having at least one window on a side, the at least one window aligned with a broken location of the bone, and the healing assembly inserted into the window in a self-locking manner towards an interior of the implant body, wherein the healing assembly has a resilient groove, the healing assembly is inserted into the window towards the interior of the implant body, and the healing assembly is snapped into the window by compressing the resilient groove.

US Pat. No. 10,521,694

3D BUILDING EXTRACTION APPARATUS, METHOD AND SYSTEM

The Chinese University of...

1. A 3D building extraction method, comprising:extracting building footprints from one or more stereo images for a building, wherein the extracting comprises:
removing non-building footprints from one or more initial building footprints of the stereo images;
obtaining, from the stereo images, building footprints height;
obtaining, from the stereo images, building footprints shadow; and
combining the building footprint height and the building footprint shadow to generate the extracted building footprints;
determining, from rational polynomial coefficients of the stereo images, first height estimation of the extracted building footprints;
obtaining, from multi-temporal synthetic aperture radar images for the building, scatters with stable attributes;
determining second height estimation of the building from the determined scatters; and
combining the first height estimation and the second height estimation to generate a fused height for each of the extracted building footprints.
US Pat. No. 10,453,556

ANALYSIS OF FRAGMENTATION PATTERNS OF CELL-FREE DNA

The Chinese University of...

1. A method of analyzing a biological sample, including a mixture of cell-free DNA molecules from a plurality of tissues types that includes a first tissue type, to determine a classification of a proportional contribution of the first tissue type in the mixture, the method comprising:identifying, by a computer system, a first set of genomic positions at which ends of cell-free DNA molecules of the first tissue type occur at a rate above a threshold;
analyzing, by the computer system, a first plurality of cell-free DNA molecules from the biological sample of a subject, wherein analyzing a cell-free DNA molecule includes:
determining a genomic position in a reference genome corresponding to at least one end of the cell-free DNA molecule;
based on the analyzing of the first plurality of cell-free DNA molecules, determining, by the computer system, that a first number of the first plurality of cell-free DNA molecules end within one of a plurality of windows, each window including at least one of the first set of genomic positions;
computing, by the computer system, a relative abundance of the first plurality of cell-free DNA molecules ending within one of the plurality of windows by normalizing the first number of the first plurality of cell-free DNA molecules using a second number of cell-free DNA molecules, wherein the second number of cell-free DNA molecules includes cell-free DNA molecules ending at a second set of genomic positions outside of the plurality of windows including the first set of genomic positions; and
determining the classification of the proportional contribution of the first tissue type by comparing the relative abundance to one or more calibration values determined from one or more calibration samples whose proportional contributions of the first tissue type are known.