US Pat. No. 9,301,520

SYSTEMS AND METHODS FOR FREEZING, STORING AND THAWING BIOPHARMACEUTICAL MATERIALS

SARTORIUS STEDIM NORTH AM...

1. A system for use in freezing, storing and thawing biopharmaceutical materials, said system comprising:
a container configured to contain biopharmaceutical materials therein;
a holder having a cavity, said container received in said cavity;
said holder comprising a first portion and a second portion forming an interior cradle, said container received between said
first portion and said second portion to connect said container to said holder;

said first portion having a first bottom and first edges extending from said first bottom and forming a first inner rim, said
first bottom and said first edges bounding said cavity, said inner rim extending completely around a perimeter of said container,
said first inner rim comprising longitudinal sides, ends, and curved corners surrounding said perimeter, each of said corners
connecting a longitudinal side of said longitudinal sides to an end of said ends, said container contacting said first bottom,
said first edges, and at least one corner of said corners;

said first edges comprising curved surfaces having first edge axes about perpendicular to axes of said curved corners;
said second portion comprising a second bottom bounding said cavity opposite said first bottom and second edges extending
from said second bottom and bounding said cavity, said second edges comprising second curved surfaces having second edge axes
about perpendicular to said axes of said curved corners, said container contacting said second bottom and said second edges;

said first portion comprising an outer rim connected to said cradle and separated from said cavity and said inner rim; and
wherein said first bottom comprises an inner surface facing said cavity receiving said container and an outer surface, said
outer surface of said first bottom being recessed towards said container relative to an outermost surface of said outer rim
such that said outermost surface is located further away from said second portion than said outer surface of said bottom is
located away from said second portion.

US Pat. No. 9,403,135

MIXING CONTAINER AND MIXING SYSTEM

Sartorius Stedim North Am...

1. A mixing system comprising:
a mixing container having a wall enclosing a mixing volume, an anchor fixed at the wall of the mixing container inside the
mixing volume, at least one mixing plate pivotably mounted to the anchor to allow a relative movement of the at least one
mixing plate along a stir direction having a component perpendicular to the extension of the mixing plate, each of the at
least one mixing plates being provided with at least one magnetic device that is mounted to a surface of the at least one
mixing plate or that is embedded in the interior of the at least one mixing plate,

a container receptacle configured to at least partially receive the mixing container, and
a magnetic actuator comprising at least one magnetic device adapted to induce a time-variant magnetic field that actuates
the at least one magnetic device of the mixing container in order to move the corresponding mixing plate along the stir direction
and a turntable to which the at least one magnetic device is mounted.

US Pat. No. 9,808,780

SYSTEM, CONTAINER, DEVICE, AND METHOD FOR MIXING

SARTORIUS STEDIM NORTH AM...

1. A mixing system, comprising
a container;
an interior mixing element in the container; and
a mixing device comprising a rigid exterior shaft having a first end section defining a first axis and a second end section
defining a second axis aligned to the first end section at an acute angle, the first end section being mounted to an exterior
drive that rotates the first end section about the first axis, the second end section being rotatably mounted in an exterior
bearing fixed to a wall of the container, an interior shaft having a first end section rotatably mounted in an interior bearing
fixed to the wall of the container and aligned at an acute angle to the first axis of the exterior shaft, the interior shaft
further having a second end section aligned at an acute angle to the first and section of the interior shaft, the interior
mixing element being fixed to the second end section of the interior shaft, wherein the exterior drive rotates the first end
section of the exterior shaft about the axis thereof and generates a nutating motion of the interior and exterior bearings,
and wherein the nutating motion of the interior bearing causes a rotating motion of the second end of the interior shaft and
the mixing element mounted thereto about the axis of the second end section of the interior shaft.

US Pat. No. 10,005,575

FACILITY AND METHOD FOR PRODUCING A CONTAINER LOADED WITH A BIOPHARMACEUTICAL FLUID

SARTORIUS STEDIM NORTH AM...

13. Method for preparing a sealed container loaded with a biopharmaceutical fluid, a gas occupying an inner filling space inside the container at an initial level of positive pressure, wherein the gas filling level within the container is controlled, the method comprising the steps of:b) estimating a gas filling control parameter which is representative of said inner filling space,
c) generating a negative pressure in the inner filling space by forcing gas to flow out of the container, the duration of the forced flow being adjusted according to said control parameter.

US Pat. No. 9,944,510

CONDUIT TERMINUS AND RELATED FLUID TRANSPORT SYSTEM AND METHOD

Sartorius Stedim North Am...

1. A conduit terminus, comprising:a body comprising a head portion and an engagement portion,
the body comprising an aperture extending along a longitudinal axis through the head portion and the engagement portion and between a first opening at the head portion and a second opening at the engagement portion,
the head portion tapering to a tip defining the first opening, the first opening having a contour that is non-planar,
wherein the head portion comprises a plurality of ribs,
wherein the head portion further comprises a stop configured to engage an end of the conduit,
wherein the ribs extend from the stop to the tip.

US Pat. No. 10,031,033

MONITORING OF THE FREEZING STATE OF A BIOPHARMACEUTICAL FLUID FOUND IN A CONTAINER

SARTORIUS STEDIM NORTH AM...

1. Monitoring device for monitoring the freezing state of a biopharmaceutical fluid in a container intended to receive a biopharmaceutical fluid that is to transition between the liquid state and the frozen state, wherein the device comprises a peripheral envelope made of plastic, intended and suitable for association with a thermal treatment receptacle,wherein the device further comprises a sensor suitable for non-intrusively detecting a control parameter which is a macroscopic parameter of the container, and an analysis system suitable for determining a freezing state of the biopharmaceutical fluid on the basis of the macroscopic parameter of the container, the macroscopic parameter being derived directly or indirectly from an average density of a non-gaseous portion of contents of the container.

US Pat. No. 9,933,113

SYSTEMS AND METHODS FOR FREEZING, STORING AND THAWING BIOPHARMACEUTICAL MATERIALS

SARTORIUS STEDIM NORTH AM...

1. A method for use in freezing, storing or thawing biopharmaceutical materials, the method comprising:providing a holder having a cavity, the holder comprising a first portion and a second portion forming an interior cradle bounding the cavity;
the first portion having a first bottom and first edges extending from the first bottom and forming a first inner rim, the first bottom and the first edges bounding the cavity, the inner rim extending completely around a perimeter of the container, the first inner rim comprising longitudinal sides, ends, and curved corners surrounding the perimeter, each of the corners connecting a longitudinal side of the longitudinal sides to an end of the ends, the container contacting the first bottom, the first edges, and at least one corner of the corners;
the second portion comprising a second bottom bounding the cavity opposite the first bottom and second edges extending from the second bottom and bounding the cavity;
the first edges and the second edges comprising concave surfaces bounding the cavity;
the holder having an outer rim connected to the cradle and separated from the cavity, and the first bottom comprising an inner surface facing the cavity and an outer surface, the outer surface of the first bottom being recessed relative to an outer surface of the outer rim; and
receiving a container for holding biopharmaceutical materials in the cavity of the holder and between the first portion and the second portion to connect the container to the holder.

US Pat. No. 9,161,527

SYSTEMS AND METHODS FOR FREEZING, STORING AND THAWING BIOPHARMACEUTICAL MATERIALS

SARTORIUS STEDIM NORTH AM...

1. A method for use in freezing, storing and thawing biopharmaceutical materials, the method comprising:
connecting a container for holding biopharmaceutical materials to a holder by receiving the container in a cavity of a cradle
of the holder, the cradle formed by a first portion and a second portion of the holder;

the first portion having a first bottom and first edges extending from the first bottom and forming a first inner rim, the
first bottom and the first edges bounding the cavity, the inner rim extending completely around a perimeter of the container,
the first inner rim comprising longitudinal sides, ends, and curved corners surrounding the perimeter, each of the corners
connecting a longitudinal side of the longitudinal sides to an end of the ends, the container contacting the first bottom,
the first edges, and at least one corner of the corners;

the second portion comprising a second bottom bounding the cavity opposite the first bottom and second edges extending from
the second bottom and bounding the cavity;

the first edges and the second edges comprising concave surfaces bounding the cavity; and
engaging a first plurality of outer teeth of an outer rim of the holder with a second plurality of outer teeth of a second
outer rim of a second holder to stack the holder and the second holder and to inhibit movement between the holder and second
holder.

US Pat. No. 10,088,106

SYSTEMS AND METHODS FOR FREEZING, STORING AND THAWING BIOPHARMACEUTICAL MATERIALS

SARTORIUS STEDIM NORTH AM...

1. A system for use in freezing, storing and thawing biopharmaceutical materials, said system comprising:a container configured to contain biopharmaceutical materials therein;
a holder having a cavity, said container received in said cavity;
said holder comprising a first portion and a second portion forming an interior cradle, said container received between said first portion and said second portion to connect said container to said holder;
said first portion having a first bottom and first edges extending from said first bottom and forming a first inner rim, said first bottom and said first edges bounding said cavity, said inner rim extending completely around a perimeter of said container, said first inner rim comprising longitudinal sides, ends, and curved corners surrounding said perimeter, each of said corners connecting a longitudinal side of said longitudinal sides to an end of said ends, said container contacting said first bottom, said first edges, and at least one corner of said corners;
said second portion comprising a second bottom bounding said cavity opposite said first bottom and second edges extending from said second bottom and bounding said cavity;
the first edges and the second edges comprising concave surfaces bounding the cavity;
said first portion comprising an outer rim connected to said cradle and separated from said cavity; and
wherein said first bottom comprises an inner surface facing said cavity receiving said container and an outer surface, said outer surface of said first bottom being recessed towards said container relative to an outermost surface of said outer rim.

US Pat. No. 9,975,753

DETACHABLE FLUID TRANSFER DEVICE ACCESSORY AND A FLUID TRANSFER ASSEMBLY

Sartorius Stedim North Am...

1. A detachable fluid transfer device accessory, comprising:a body, comprising:
an upper extension defining a lower engagement surface;
a lower extension defining an upwardly extending protrusion; and
a connector portion extending between the upper extension and the lower extension; and
a resilient engagement member coupled to the lower engagement surface of the body,
the upper extension, the lower extension, and the connector portion collectively defining a cavity configured to receive a tab assembly of a fluid transfer device,
the upwardly extending protrusion being configured to engage a housing of the fluid transfer device,
the resilient engagement member being configured to engage an upper surface of the tab assembly to retain the tab assembly in a fixed configuration relative to the housing.
US Pat. No. 10,208,283

METHODS AND SYSTEMS FOR MANIPULATING PARTICLES USING A FLUIDIZED BED

Sartorius Stedim North Am...

1. A method for manipulating particles using a fluidized bed, the method comprising:rotating a chamber about a substantially horizontal axis to create a centrifugal force field, the chamber comprising an inlet and an outlet;
flowing a first stream containing a first media and particles into the chamber through the inlet, wherein flowing the first stream acts to create a force which opposes the centrifugal force;
forming a fluidized bed of particles in the chamber, wherein the forces substantially immobilize the particles in the fluidized bed by the summation of vector forces acting on the particles;
collecting the first media substantially without particles passing through the outlet of the chamber; then
manipulating the particles in the fluidized bed, wherein said manipulating is selected from the group consisting of removing, concentrating, diluting, exchanging media, harvesting, transferring, dispensing, transfecting, electroporating, separating, extracting, isolating, selecting, purifying, coating, binding, physically modifying, and altering the environment; and thereafter
removing the particles from the fluidized bed, wherein removing the particles comprises:
flowing a second stream into the chamber through the outlet, wherein flowing the second stream acts to create a force at least partially in the same direction as the centrifugal force field; and
collecting the particles passing through the inlet of the chamber.

US Pat. No. 10,486,959

FLUID TRANSFER INTERFACE

Sartorius Stedim North Am...

1. A fluid transfer interface for use with a vessel, the interface comprising:a body,
one or more apertures extending through the body, and
one or more fluid transfer conduits secured to the body by way of a cast seal and extending continuously and axially through the one or more apertures, the one or more fluid transfer conduits comprising at least one of a thermoset or a thermoplastic,
the cast seal comprising a vulcanizable casting agent cured after the one or more fluid transfer conduits are inserted through the one or more apertures.

US Pat. No. 10,773,863

VESSEL CLOSURES AND METHODS FOR USING AND MANUFACTURING SAME

Sartorius Stedim North Am...

1. A fluid transfer hub, comprising:a first vessel closure;
a second vessel closure;
a gasket arranged between the first vessel closure and the second vessel closure; and
a clamp at least partially surrounding the first vessel closure, the second vessel closure and the gasket,
wherein the first vessel closure comprises:
a first body, one or more apertures extending axially through the first body, one or more first inserts extending axially through the one or more apertures, and a cast seal disposed within the first body and surrounding each first insert,
wherein the second vessel closure comprises:
a second body, one or more apertures extending axially through the second body, one or more second inserts extending axially through the one or more apertures, and a cast seal disposed within the second body and surrounding each second insert.

US Pat. No. 10,888,878

METHODS, SYSTEMS AND APPARATUS FOR MANIPULATING PARTICLES

Sartorius Stedim North Am...

1. An apparatus for manipulating particles, the apparatus comprising:a rotor rotatable at a speed about an axis, the rotor having an outer periphery and front and rear opposite sides;
at least one chamber mounted on the rotor, each chamber having an inlet and an opposed outlet;
an umbilical assembly rotatable about the axis, the umbilical assembly comprising:
a curvilinear guide connecting to the rear side of the rotor;
a flexible conduit residing in the guide; and
first and second elongate passageways for each chamber extending through the conduit, wherein the first passageway is in fluid communication with the inlet of a respective chamber and the second passageway is in fluid communication with the outlet of the respective chamber; and
a drive mechanism configured to rotate the umbilical assembly at about one-half the speed of the rotor;
wherein each chamber comprises a substantially conical body portion and a flange extending about at least a portion of a perimeter of the conical body portion, the flange defining a plane and the conical body portion extending outwardly from the plane, the flange including an inlet fluid path that is external to the conical body portion and extends around at least a portion of the perimeter of the conical body portion to the chamber inlet and an outlet fluid path that extends from the chamber outlet, and wherein the first elongate passageway is in fluid communication with the flange inlet fluid path and the second elongate passageway is in fluid communication with the flange outlet fluid path,
wherein each chamber is configured to allow fluid flow therethrough while the rotor and the chamber rotate about the axis such that a force of fluid flowing from the inlet to the outlet opposes a centrifugal force created by the rotation of the rotor and the chamber,
wherein a fluid flow path from the inlet to the outlet is perpendicular to the axis.
US Pat. No. 10,799,869

FLUID TRANSPORT APPARATUS, FLEXIBLE CONDUIT, METHOD OF MANUFACTURING FLEXIBLE CONDUIT, AND STORAGE METHOD

Sartorius Stedim North Am...

11. A storage method, comprising:providing a biological material;
providing a fluid transport apparatus defining an aseptic pathway configured to direct fluids therethrough, the fluid transport apparatus comprising:
a conduit having a first terminus and a second terminus, the conduit extending between the first terminus and the second terminus, the conduit formed of platinum catalyzed silicone rubber having methyl and phenyl side groups, the conduit having a storage modulus of 108 or less Pascals at ?75 degrees Celsius;
at least one fitting connected to the first terminus; and
a vessel engaged with the second terminus;
directing the biological material through the conduit into the vessel;
sealing the vessel at the conduit; and
cryogenically storing the biological material in the vessel.

US Pat. No. 11,027,108

FLUID TRANSFER ASSEMBLY WITH A JUNCTION HAVING MULTIPLE FLUID PATHWAYS

Sartorius Stedim North Am...

1. A fluid transfer assembly, comprising:a unitary junction having an upstream portion and a downstream portion, the unitary junction defining a plurality of curved fluid pathways between the upstream portion and the downstream portion, each of the curved fluid pathways defining a central axis along a length thereof, the central axis comprising a linear first segment, a curved second segment, and a linear third segment, the second segment disposed between the first and third segments, the second segment of the central axis deviating from a straight line to define a smooth curve, a linear projection of the first segment intersecting a linear projection of the third segment outside of the respective curved fluid pathway or the first and third segments being parallel to one another; and
at least one flexible fluid conduit sealed to the junction in fluid communication with at least one of the plurality of curved fluid pathways,
wherein fluid is transferred from a primary vessel to a secondary vessel through the unitary junction.