US Pat. No. 9,120,298

METHOD OF CONTINUOUSLY MANUFACTURING MICROFLUIDIC CHIPS WITH BOPET FILM FOR A MICROFLUIDIC DEVICE AND MICROFLUIDIC CHIPS WITH BOPET FILM

FluxErgy, LLC, San Diego...

1. A method for manufacturing a device, comprising:
coating UV epoxy on a first side of a BoPET film;
placing the BoPET film with UV epoxy coated thereon on a first substrate with the first side facing the first substrate;
curing the UV epoxy on the first side of the BoPET film to attach the BoPET film on the first substrate;
forming at least one microfluidic pathway in the BoPET film that is attached on the first substrate;
coating UV epoxy on a first side of a second substrate;
placing the second substrate on the BoPET film with the first side of the second substrate facing a second side of the BoPET
film; and

curing the UV epoxy on the first side of the second substrate to attach the BoPET film that includes the microfluidic pathway
formed therein to the second substrate.

US Pat. No. 9,180,652

MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING AND A METHOD OF MANUFACTURING MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING FOR A MICROFLUIDIC DEVICE

FluxErgy, LLC, San Diego...

1. A device, comprising:
a substrate having a first thickness;
at least one microfluidic pathway in the substrate, wherein the microfluidic pathway includes channels etched in the substrate;
a coating along the microfluidic pathway, and a glass layer having a second thickness on the substrate and above the microfluidic
pathway, wherein the coating contains cyanoacrylates, wherein the coating is configured to be coated along the channels while
not contacting the glass layer in the microfluidic pathway, said coating thereby not filling the channels;

wherein the coating is configured to seal the channels of the microfluidic pathway, while not filling the channels,
wherein the coating is not configured to be used as an adhesive in the channels of the substrate, and
the first thickness is greater than the second thickness.

US Pat. No. 9,636,674

MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING AND A METHOD OF MANUFACTURING MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING FOR A MICROFLUIDIC DEVICE

FluxErgy, LLC, Tustin, C...

17. A method of manufacturing a microfluidic device comprising:
etching a first layer of the microfluidic device to form at least one microfluidic pathway in the first layer;
coating the at least one microfluidic pathway with a coating to reduce fluid diffusion;
allowing the coating to set so that the coating is exposed along the at least one microfluidic pathway; and
bonding a second layer to the first layer so that the at least one microfluidic pathway is located between the first layer
and the second layer and so that the coating is exposed to reduce fluid diffusion of a fluid sample flowing through the at
least one microfluidic pathway and seal a surface of the at least one microfluidic pathway.

US Pat. No. 10,137,448

MICROFLUIDIC CHIP WITH COATING TO REDUCE FLUID DIFFUSION AND METHOD OF MANUFACTURING SAME

FluxErgy, LLC, Irvine, C...

1. A microfluidic chip comprising:a body including at least one microfluidic pathway configured to receive a fluid sample, the at least one microfluidic pathway including a coating configured to reduce fluid diffusion and seal a surface of the at least one microfluidic pathway; and
a heating device located on the body and forming a heating zone within a portion of the at least one microfluidic pathway.

US Pat. No. 10,214,772

TEST CARD FOR ASSAY AND METHOD OF MANUFACTURING SAME

FluxErgy, LLC, Irvine, C...

1. A method of manufacturing a test card for analysing a fluid sample, comprising:cutting at least a microchannel into a first polymer material to form a channel layer;
cutting a first aperture and a second aperture into a second polymer material to form a first substrate layer;
bonding the first substrate layer to a first side of the channel layer and a second substrate layer to a second side of the channel layer to form a single bonded layer with the microchannel passing therethrough and placing the first aperture and the second aperture in fluid communication with each other;
forming a third substrate layer to be thicker than the first substrate layer and the second substrate layer;
printing a conductive ink onto a first side of the bonded layer; and
adhering the third substrate layer to a second side of the bonded layer opposite the first side to form the test card, wherein the formed test card has a first surface formed by third substrate layer and an opposite second surface with the conductive ink exposed for contact with an outside source of current.