US Pat. No. 9,062,291

TRANSGLUTAMINASE HAVING DISULFIDE BOND INTRODUCED THEREIN

AJINOMOTO CO., INC., Tok...

1. A protein possessing transglutaminase activity selected from the group consisting of:
(A) a protein comprising the amino acid sequence of SEQ ID NO: 2 or a mutant thereof that is at least 70% homologous to the
amino acid sequence of SEQ ID NO: 2, but wherein said protein has a mutation selected from the group consisting of:

a) substitution of the amino acids at positions 7 and 58 with cysteine,
b) substitution of the amino acids at positions 46 and 318 with cysteine,
c) substitution of the amino acids at positions 93 and 112 with cysteine,
d) substitution of the amino acids at position 106 and 213 with cysteine,
e) substitution of the amino acids at positions 160 and 228 with cysteine,
f) substitution of the amino acids at positions 2 and 282 with cysteine,
g) substitution of the amino acids at positions 2 and 283 with cysteine,
h) substitution of the amino acids at positions 3 and 283 with cysteine, and
i) substitution of the amino acids at positions 17 and 330 with cysteine;
(B) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 4, 6, 8, 10, and 12, or mutants
thereof that are at least 70% homologous to the amino acid sequences of SEQ ID NO: 4, 6, 8, 10, and 12, respectively, wherein
said protein has a mutation selected from the group consisting of:

a) substitution of the amino acids at positions 7 and 58 with cysteine,
b) substitution of the amino acids at positions 46 and 318 with cysteine,
c) substitution of the amino acids at positions 93 and 112 with cysteine,
d) substitution of the amino acids at position 106 and 213 with cysteine,
e) substitution of the amino acids at positions 160 and 228 with cysteine,
f) substitution of the amino acids at positions 2 and 282 with cysteine,
g) substitution of the amino acids at positions 2 and 283 with cysteine,
h) substitution of the amino acids at positions 3 and 283 with cysteine, and
i) substitution of the amino acids at positions 17 and 330 with cysteine;
wherein said positions correspond to those in SEQ ID NO: 2.

US Pat. No. 9,060,456

PRODUCTION METHOD OF MULTILAYER PRINTED WIRING BOARD AND MULTILAYER PRINTED WIRING BOARD

AJINOMOTO CO., INC., Tok...

1. A multilayer printed wiring board, comprising:
an insulating layer formed by a prepreg comprised of a glass cloth impregnated with a thermosetting resin composition,
a via hole formed on the insulating layer,
a circuit containing a via formed by conductive layer in the via hole, and
a glass cloth protruding in a length of not more than 4 ?m from the sidewall of the aforementioned via hole,
wherein said via hole has a top diameter of not more than 75 ?m, and
wherein the protruding part of the glass cloth is embedded in the conductive layer forming the via.

US Pat. No. 9,150,505

PROPHYLACTIC OR THERAPEUTIC AGENT FOR DIABETES OR OBESITY

AJINOMOTO CO., INC., Tok...

1. A method for treating diabetes or obesity, comprising administering to a subject in need thereof a compound represented
by Formula (I) or a salt thereof:

wherein, R1 and R2, each independently, represent a hydrogen atom or unsubstituted C1-6 alkyl;

R3 represents a hydrogen atom;

R4 and R5, each independently, represent a hydrogen atom or C1-6 alkyl;

X represents CH2, an oxygen atom, NH, or a sulfur atom;

Y represents C?O, SO, SO2, or C?S;

R6 represents a hydrogen atom;

G represents R7-substituted phenyl or R7-substituted pyridyl, where the R7-substituted phenyl or the R7-substituted pyridyl may further be substituted with one or two R8;

R7 represents sulfo or carboxyl;

R8 represents C1-6 alkyl, halogeno, hydroxy, C1-6 alkoxy, nitro, sulfo, or C1-3 alkylcarbonylamino, where they may be different when more than one R8 exist;

Q represents a hydrogen atom, substituted or unsubstituted C1-6 alkyl, carboxyl, CONReRf, CONHNHRg, CORh, phenyl or substituted or unsubstituted oxadiazolyl;

Re and Rf, each independently, represent a hydrogen atom, substituted or unsubstituted C1-6 alkyl, C1-6 alkylsulfonyl, phenylsulfonyl, C3-8 cycloalkyl, or hydroxy, or alternatively, Re and Rf may integrally form morpholino;

Rg represents substituted or unsubstituted C1-6 alkylcarbonyl; and

Rh represents C1-6 alkoxy,

provided that when X is methylene or an oxygen atom, Y is C?O, all of R1-R5 are hydrogen atoms and G is R7-substituted phenyl optionally substituted with one or two R8, then, Q is a group other than carboxyl or CORh.

US Pat. No. 9,420,813

SWEETENER

AJINOMOTO CO., INC., Tok...

1. A composition comprising a sweetener and a compound; wherein the compound is selected from the group consisting of ?-Glu-X-Gly,
?-Glu-Val-Y, and combinations thereof; wherein X is selected from the group consisting of Gly, Abu, t-Leu, Cle, Aib, Pen and
Ser; and Y is selected from the group consisting of Gly, Val, Glu, Lys, Phe, Ser, Pro, Arg, Asp, Met, Thr, His, Orn, Asn,
Cys, Gln, GlyA and LacA;
wherein the compound is present in the composition in an amount of from 0.000001% to 99.9999% by weight.

US Pat. No. 9,516,765

METHOD FOR PRODUCING PRINTED WIRING BOARD

Ajinomoto Co., Inc., Chu...

1. A method for producing a printed wiring board, comprising (A) to (F) in this order:
(A) laminating, onto an internal layer circuit substrate, a resin sheet with a support which comprises a support and a resin
composition layer in contact with the support, so that the resin composition layer is in contact with said internal layer
circuit substrate;

(B) thermally curing said resin composition layer of said resin sheet with a support to form an insulating layer;
(C) perforating said insulating layer to form a via hole;
(D) performing a desmear treatment;
(E) peeling said support, to expose a surface of said insulating layer; and
(F) forming a conductive layer on the surface of said insulating layer,
wherein
said resin composition layer of said resin sheet with a support comprises at least one epoxy resin, at least one curing agent,
and at least one inorganic filler,

said at least one curing agent comprises an active ester-based curing agent and a curing agent selected from the group consisting
of a phenol-based curing agent and a naphthol-based curing agent,

said at least one inorganic filler is treated with at least one surface treatment agent selected from the group consisting
of an organosilazane compound, a titanate-based coupling agent, an aminosilane-based coupling agent, an epoxysilane-based
coupling agent, and a mercaptosilane-based coupling agent, and

said at least one inorganic filler is present in said resin composition layer in an amount of 72% by mass to 95% by mass,
when a content of nonvolatile components in said resin composition layer is defined as 100% by mass.

US Pat. No. 9,341,616

SWEET TASTE RECEPTOR CHIMERIC PROTEINS AND USE THEREOF

AJINOMOTO CO., INC., Tok...

1. A method for detecting a sweet taste substance or a sweet taste-regulating substance comprising the steps of:
contacting a test substance with a cell that expresses a T1R2 protein and a T1R3 protein, and
detecting an interaction of the T1R2 protein and/or T1R3 protein and the test substance, wherein
a) the T1R2 protein is selected from the group consisting of:
a1) a chimeric T1R2 protein comprising the region of positions 1 to 470 of the human T1R2 protein, and the region of positions
475 to 843 of the mouse T1R2 protein, which are fused in this order,

a2) a chimeric T1R2 protein comprising the region of positions 1 to 480 of the human T1R2 protein, and the region of positions
485 to 843 of the mouse T1R2 protein, which are fused in this order, and

a3) a chimeric T1R2 protein comprising the region of positions 1 to 489 of the human T1R2 protein, and the region of positions
494 to 843 of the mouse T1R2 protein, which are fused in this order,

and wherein
b) the T1R3 protein is selected from the group consisting of:
b1) a chimeric T1R3 protein comprising the region of positions 1 to 63 of the human T1R3 protein, the region of positions
64 to 539 of the mouse T1R3 protein, and the region of positions 535 to 852 of the human T1R3 protein, which are fused in
this order,

b2) a chimeric T1R3 protein comprising the region of positions 1 to 63 of the mouse T1R3 protein, the region of positions
64 to 162 of the human T1R3 protein, the region of positions 163 to 539 of the mouse T1R3 protein, and the region of positions
535 to 852 of the human T1R3 protein, which are fused in this order, and

b3) a chimeric T1R3 protein comprising the region of positions 1 to 162 of the mouse T1R3 protein, the region of positions
163 to 242 of the human T1R3 protein, the region of positions 243 to 539 of the mouse T1R3 protein, and the region of positions
535 to 852 of the human T1R3 protein, which are fused in this order.

US Pat. No. 9,045,789

METHOD FOR PRODUCING A TARGET SUBSTANCE BY FERMENTATION

AJINOMOTO CO., INC., Tok...

1. A method for producing a target substance comprising
A) culturing a bacterium belonging to the genus Pantoea or the genus Corynebacterium and having an ability to produce the target substance in a medium containing xylose wherein the bacterium is able to produce
the target substance through conversion of xylonic acid produced from xylose into 2-ketoglutaric acid by xylonate dehydratase,
2-keto-3-deoxyxylonate dehydratase, and 2-ketoglutaric semialdehyde dehydrogenase,

B) allowing the bacterium to produce and secrete the target substance into the medium, and
C) collecting the target substance from the medium,
wherein the target substance is 2-ketoglutaric acid or a derivative thereof, the bacterium has an ability to produce xylonic
acid from xylose, and activities of the enzymes xylonate dehydratase, 2-keto-3-deoxyxylonate dehydratase, and 2-ketoglutaric
semialdehyde dehydrogenase have been imparted to or enhanced in the bacterium;

wherein the 2-ketoglutaric acid derivative is selected from the group consisting of L-glutamic acid, L-glutamine, L-arginine,
L-citrulline, L-ornithine, L-proline, putrescine, and ?-aminobutyric acid,

wherein the bacterium can produce xylonic acid from xylose because of any one of the following characteristics:
(i) xylose dehydrogenase activity, or xylose dehydrogenase activity and xylonolactonase activity have been imparted to or
enhanced in the bacterium, or

(ii) the bacterium has glucose dehydrogenase activity that can catalyze a reaction producing xylonic acid from xylose,
wherein said activities are imparted to or enhanced in the bacterium by introducing expressable forms of genes coding for
the enzymes into the bacterium, wherein the genes are native to a microorganism belonging to a genus selected from the group
consisting of Caulobacter, Escherichia, Agrobacterium, Herbaspirillum, Actinoplanes, Cupriavidus, Pseudomonas, Zobellia, Thermobacillus,
Arthrobacter, Azospirillum, Halomonas, Bacillus, and Aspergillus.

US Pat. No. 9,450,174

PIEZOELECTRIC ELEMENT

AJINOMOTO CO., INC., Tok...

1. A piezoelectric element, comprising at least one poly(?-amino acid), which contains:
(A) glutamic acid ?-ester units represented by formula (I):
wherein R1 is a methyl group or a benzyl group; and
(B) units of one or more types selected from the group consisting of:
(1) a glutamic acid ?-ester unit represented by formula (II):

wherein R2 is an unsubstituted alkyl group having 6 to 18 carbon atoms, or a substituted alkyl group having 1 to 6 carbon atoms, in which
the hydrogen atoms are partly or entirely substituted by a halogen atom or an alicyclic hydrocarbon group having 3 to 12 carbon
atoms;

(2) an alanine unit, a phenylalanine unit, and an N?-benzyloxycarbonyllysine unit, which are represented by formula (III):


wherein R3 is a methyl group, a benzyl group or a (CH2)4—NHZ group, in which Z is a benzyloxycarbonyl group; and

(3) a glutamic acid ?-ester unit represented by formula (IV):

wherein each R4 may be the same or different and is an alkoxy group having 1 to 12 carbon atoms; an alkyl group having 1 to 12 carbon atoms,
in which the hydrogen atoms are partly or entirely substituted by a halogen atom; or an alkylcarbonyl group, in which the
alkyl group has 1 to 12 carbon atoms; l is an integer of 6 to 12; and m is an integer of 1 to 3.

US Pat. No. 9,399,030

TOPICALLY APPLIED CIRCULATION ENHANCING AGENT AND SKIN AND HAIR COSMETIC AND BATH AGENT CONTAINING THE SAME

AJINOMOTO CO., INC., Tok...

1. A method of enhancing blood circulation in a subject in need of enhanced blood circulation comprising topically administering
an agent comprising a capsinoid compound, wherein the concentration of said capsinoid compound in said agent is from 0.5 weight
% to 5.0 weight %.
US Pat. No. 9,234,223

METHOD FOR PRODUCING L-CYSTEINE

AJINOMOTO CO., INC., Tok...

1. A method for producing L-cysteine, a related substance thereof, or a mixture thereof, which comprises:
A) culturing a bacterium belonging to the genus Escherichia in a medium comprising thiosulfate, which bacterium has L-cysteine-producing ability and is modified so that expression of
a gene involved in sulfite reduction is increased by a method selected from the group consisting of increasing copy number
of the gene involved in sulfite reduction, modifying an expression control sequence of the gene, and combinations thereof;
and

B) collecting L-cysteine, a related substance thereof, or a mixture thereof from the medium,
wherein the gene involved in sulfite reduction is a cysG gene, and
wherein the cysG gene comprises a DNA selected from the group consisting of:
(a) a DNA comprising the nucleotide sequence of SEQ ID NO: 47 or 49,
(b) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 48 or 50, and
(c) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 48 or 50, but which includes one to 10 amino
acid substitutions, deletions, insertions, or additions.

US Pat. No. 9,216,946

METHOD OF PRODUCING BASIC AMINO ACID OR BASIC AMINO ACID SALT

AJINOMOTO CO., INC., Chu...

1. A method of producing a basic amino acid or a basic amino acid salt which comprises preparing a solution comprising a weak
acid and a HCl salt of a basic amino acid to provide a weak acid salt of the basic dechlorinating by passing the solution
through an anion-exchange resin to prepare said basic amino acid or basic amino acid salt.
US Pat. No. 9,458,206

L-AMINO ACID-PRODUCING BACTERIUM AND A METHOD FOR PRODUCING AN L-AMINO ACID

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid comprising:
a) culturing in a medium a bacterium belonging to the family Enterobacteriaceae, which is able to produce an L-amino acid,
and has been modified to have a mutation in a yeaS gene selected from the group consisting of:

(I) replacing the amino acid residue at position 28 of SEQ ID NO: 2 with an amino acid residue other than threonine,
(II) replacing the amino acid residue at position 137 of SEQ ID NO: 2 with an amino acid residue other than phenylalanine
residue,

(III) replacing the amino acid residue at position 188 of SEQ ID NO: 2 with an amino acid residue other than leucine, and,
(IV) combinations thereof; and
b) collecting the L-amino acid from the medium;
wherein the yeaS gene without said mutation encodes a protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence of SEQ ID NO: 2, and
(B) a protein comprising the amino acid sequence of SEQ ID NO: 2, but wherein 1 to 10 amino acid residues are substituted,
and the protein has an activity of improving an L-cysteine-producing ability in the bacterium belonging to the family Enterobacteriaceae
as compared to a non-modified bacterium when expression of the protein is increased in the bacterium.

US Pat. No. 9,420,817

AGENT FOR AMELIORATION OF DYSPHAGIA, AND PHARMACEUTICAL OR FOOD COMPOSITION COMPRISING THE SAME

AJINOMOTO CO., INC., Tok...

1. A method of ameliorating dysphagia comprising administering a composition containing an effective amount of a capsinoid
to a subject suffering from dysphagia, wherein said composition contains capsaicinoid in an amount such that amelioration
of dysphagia by administering said composition is provided without causing pungency, and wherein said administering is orally
and said composition is a gum or a troche.
US Pat. No. 9,062,172

RESIN COMPOSITION ADHESIVE FILM AND PREPREG CONTAINING THE SAME, MULTILAYERED PRINTED WIRING BOARD CONTAINING AN INSULATING LAYER FORMED BY CURING SUCH A RESIN COMPOSITION, SEMICONDUCTOR DEVICE CONTAINING SUCH A MULTILAYERED PRINT

AJINOMOTO CO., INC., Tok...

1. A resin composition, comprising (A) a cyanate ester resin, (B) a naphthylene ether type epoxy resin, (C) an inorganic filler,
and (F) an active ester curing agent,
wherein said naphthylene ether type epoxy resin contains at least one ether linkage directly connecting two naphthylene rings,
wherein, when a non-volatile matter content in said resin composition is defined as 100% by mass, said inorganic filler is
present in an amount of 52% or more by mass,

wherein a cured material of the resin composition has a surface roughness of 270 nm or less,
wherein the cured material of the resin composition has a dielectric dissipation factor of 0.008 or less, and
wherein said (F) an active ester curing agent is a phenol ester compound, a thiophenol ester compound, an N-hydroxyamine ester
compound, or a compound in which a heterocyclic hydroxyl group is esterified.

US Pat. No. 9,051,591

BACTERIUM OF ENTEROBACTERIACEAE FAMILY PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES

AJINOMOTO CO., INC., Tok...

1. A method for producing L-aspartic acid or an L-aspartic acid-derived metabolite comprising:
cultivating a bacterium belonging to the genus Pantoea or Escherichia, which produces L-aspartic acid or an L-aspartic acid-derived metabolite, in a culture medium to produce and excrete the
L-aspartic acid or the L-aspartic acid-derived metabolite into the medium, and collecting L-aspartic acid or said L-aspartic
acid-derived metabolite from the medium,

wherein said bacterium has been modified by introducing a gene encoding aspartate dehydrogenase derived from Bradyrhizobium japonicum wherein the gene encodes an amino acid sequence not less than 95% identical to the amino acid sequence encoded by SEQ ID NO:
68, and

wherein said L-aspartic acid-derived metabolite is selected from the group consisting of L-threonine, L-lysine, L-arginine,
L-methionine and L-homoserine.

US Pat. No. 9,476,076

METHOD FOR PRODUCING L-AMINO ACID USING MICROORGANISM HAVING INCREASED PHOSPHATE TRANSPORTER ACTIVITY

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid comprising:
(A) culturing a coryneform bacterium having an L-amino acid producing ability in a medium; and
(B) collecting the L-amino acid from the medium,
wherein the bacterium has been modified to increase the expression of a gene encoding a phosphate transporter, wherein the
increased expression of the gene is obtained by increasing the copy number of the gene and/or modifying an expression control
sequence of the gene, wherein said phosphate transporter is selected from the group consisting of:

(a) a protein which comprises the amino acid sequence of SEQ ID NO: 6 or SEQ ID NO: 26,
(b) a protein which comprises all of SEQ ID NO: 6 or 26 except for the substitution, deletion, insertion or addition of 1
to 10 amino acid residues, wherein said protein has phosphate transporter activity,

(c) a protein which comprises all of SEQ ID NO: 6 or 26 except for the replacement of the amino acid residue at a position
corresponding to position 246 of SEQ ID NO: 6 with a serine residue, and

(d) a protein which comprises all of SEQ ID NO: 6 or 26 except for (i) the replacement of the amino acid residue at a position
corresponding to position 246 of SEQ ID NO: 6 with a serine residue, and (ii) the substitution, deletion, insertion or addition
of 1 to 10 amino acid residues, wherein said protein has phosphate transporter activity.

US Pat. No. 9,346,821

HETEROCYCLIC CARBOXYLIC ACID ESTER DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. A compound represented by formula (I), or a pharmaceutically acceptable salt thereof:
wherein
D is a benzene ring, a naphthalene ring or a pyridine ring;
Het is a hetero ring represented by formula (III-1)

wherein Z1 and Z2 may be the same or different and is each independently CRa and Z3 is an oxygen atom, wherein Ra is independently
a hydrogen atom, a nitro group, a halogen atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino
group, a formyl group, a lower alkyl group, a lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group,
a sulfo group, a phosphono group, a lower alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower acyloxy
group, a lower acylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower alkylcarbamoyl group, a lower alkylsulfonylamino
group or a sulfamoyl group;

R1 is a hydrogen atom, a nitro group, a halogen atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino
group, a formyl group, a lower alkyl group, a lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group,
a sulfo group, a phosphono group, a lower alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower acyloxy
group, a lower acylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower alkylcarbamoyl group, a lower alkylsulfonylamino
group, or a sulfamoyl group;

n is an integer of 0 to 3;
each R2 is independently a nitro group, a halogen atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a
guanidino group, a formyl group, a lower alkyl group, a lower alkenyl group, a lower alkynyl group, a lower acyl group, a
carboxyl group, a sulfo group, a phosphono group, a lower alkoxyl group, a lower alkylthio group, a lower alkylamino group,
a lower acyloxy group, a lower acylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower alkylcarbamoyl group,
a lower alkylsulfonylamino group, or a sulfamoyl group;

X is a lower alkylene group optionally having one or more substituents selected from the group consisting of a halogen atom,
a hydroxyl group, an amino group, a lower alkoxyl group, a lower acyl group, and an oxo group, provided when said lower alkylene
group has one or more substituents and A is CO2R6, then said one or more substituents is other than an oxo group;

Z is —N(R3)- wherein R3 is a hydrogen atom, a lower alkyl group optionally having substituent(s) selected from the group consisting
of a a carboxyl group and —CONH—CH2—CO2H; a lower alkenyl group optionally having substituent(s) selected from the group consisting of a a carboxyl group and —CONH—CH2-CO2H;
or a lower cycloalkyl group optionally having substituent(s) selected from the group consisting of a carboxyl group and —CONH—CH2-CO2H;

Y is a single bond or —(CH2)p—C(R4a)(R4b)-(CH2)q— wherein R4a and R4b are each independently a hydrogen atom, a lower alkyl group, or an aralkyl group, p and q are each an
integer of 0 to 5, and p+q is an integer of 0 to 5;

A is —CO2R6 wherein R6 is a hydrogen atom, or a group represented by the formula (II)

wherein
R5 is a hydrogen atom, a lower alkyl group optionally having substituent(s) selected from the group consisting of a nitro
group, a halogen atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group,
a phenyl group, a lower alkyl group, a lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a
sulfo group, a phosphono group, a lower alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl
group, a carbamoyl group, a lower alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, a lower alkenyl group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen atom,
a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a phenyl group, a lower
alkyl group, a lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono
group, a lower alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl
group, a lower alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, or a lower alkynyl group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen
atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a phenyl group, a
lower alkyl group, a lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono
group, a lower alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl
group, a lower alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H;

Q is a lower alkylene group optionally having one or more substituents selected from the group consisting of a nitro group,
a halogen atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower
acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower alkoxyl group, a lower alkylthio group, a lower alkylamino
group, a lower acyloxy group, a lower acylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower alkylcarbamoyl
group, a lower alkylsulfonylamino group, an arylsulfonylamino group optionally having substituent(s) selected from the group
consisting of a nitro group, a halogen atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group,
a formyl group, a lower alkyl group, a lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a
sulfo group, a phosphono group, a lower alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl
group, a carbamoyl group, a lower alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, a lower cycloalkyl group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen
atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group,
a lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower
alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, an aryl group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen atom, a cyano
group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group, a lower alkenyl
group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower alkoxyl group,
a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower alkylcarbamoyl
group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, an aryloxy group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen atom,
a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group, a
lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower
alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, an arylthio group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen atom,
a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group, a
lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower
alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, an aralkyl group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen atom,
a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group, a
lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower
alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, an aralkyloxy group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen atom,
a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group, a
lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower
alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, an aralkylthio group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen atom,
a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group, a
lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower
alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, a heterocyclic group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen atom,
a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group, a
lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower
alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, a heterocyclic oxy group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen
atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group,
a lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower
alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, a heterocyclic thio group optionally having substituent(s) selected from the group consisting of a nitro group, a halogen
atom, a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group,
a lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower alkoxycarbonyl group, a carbamoyl group, a lower
alkylcarbamoyl group, a lower alkylsulfonylamino group, a sulfamoyl group, and —CONH—CH2—CO2H, and an oxo group; and

R7 is a hydrogen atom, or
R2 and R3 are optionally bonded together to form tetrahydropyridine;
R3 and R4a are optionally bonded together to form a hetero ring selected from the group consisting of pyrrolidine, piperidine,
thiazolidine, and tetrahydroisoquinoline;

R3 and R4a and R4b are optionally bonded together to form pyrrole; and
R4a and R4b are optionally bonded together to form lower cycloalkane.
US Pat. No. 9,212,154

CYSTEINE DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. A compound selected from the group consisting of N-acetyl-2-methylthiazolidine-2,4-dicarboxylic acid 2-ethyl ester and
a salt thereof.

US Pat. No. 9,204,660

FEED ADDITIVE COMPOSITION FOR RUMINANTS AND METHOD OF PRODUCING THE SAME

AJINOMOTO CO., INC., Tok...

1. A method of producing a feed additive composition, comprising:
preparing a molten mixture of at least one protective agent, lecithin and at least one of L-lysine and a salt of L-lysine;
solidifying the molten mixture by immersing the molten mixture in water or an aqueous liquid;
adjusting a water activity of the solidified mixture to 0.25 to 0.6;
wherein:
the at least one protective agent comprises at least one member selected from the group consisting of a hydrogenated vegetable
oil having a melting point of greater than 50° C. and less than 90° C. and a hydrogenated animal oil having a melting point
of greater than 50° C. and less than 90° C.;

the at least one protective agent is present in the molten mixture in an amount of from 39.5 to 59.5% by weight;
the lecithin is present in the molten mixture in an amount of from 0.05 to 5% by weight; and
the at least one of L-lysine and the salt of L-lysine is present in the molten mixture in an amount of from 40 to 60% by weight.
US Pat. No. 9,062,335

METHOD FOR PRODUCING BASIC SUBSTANCE

AJINOMOTO CO., INC., Tok...

1. A method for producing a basic amino acid by fermentation comprising culturing a microorganism having an ability to produce
the basic amino acid in a liquid medium contained in a fermentation tank to produce and accumulate the basic amino acid in
the medium;
wherein the amount of sulfate and/or chloride ions used as counter ions of the basic amino acid is reduced by adjusting the
total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total
period of the culture process including a period where the pH of the medium increases due to shortage of the counter ions
caused by accumulation of the objective basic amino acid;

wherein the total ammonia concentration in the medium is adjusted to be 300 mM or lower; and
wherein the microorganism is a coryneform bacterium.

US Pat. No. 9,198,846

BASIC AMINO ACID DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. A compound represented by formula (1):
wherein
R1—CO— is a lauroyl group;

R2 is a hydrogen atom or a saturated or unsaturated, straight chain or branched chain hydrocarbon group having 1 to 15 carbon
atoms;

m is 4;
n is an integer of 0 to 9; and
R3 and R4 are each independently a hydrogen atom, or a saturated or unsaturated, straight chain or branched chain hydrocarbon group
having 1 to 6 carbon atoms,

or a salt thereof.

US Pat. No. 9,102,630

CRYSTALS OF SALTS OF PHENYLALANINE DERIVATIVES

AJINOMOTO CO., INC., Chu...

1. A method of treating rheumatoid arthritis, inflammatory, intestinal diseases, systemic lupus erythematosus, disseminated
or multiple sclerosis, Sjogren's syndromes, asthma, psoriasis, allergy, diabetes (mellitus), cardiovascular diseases, arterial
sclerosis, restenosis, tumor hyperplasia, tumor metastasis, or graft rejection, comprising administering to a subject in need
thereof an effective amount of a the compound represented by formula (I):
or a pharmaceutically-acceptable acid addition salt thereof, and

at least one additional pharmaceutical agent selected from the group consisting of 5-ASA pharmaceutical preparations, adrenocortical
hormone-containing pharmaceutical preparations, antibacterial agents, immunosuppressive agents and anti-cytokine agents.

US Pat. No. 9,062,334

METHOD FOR PRODUCING PYRROLOQUINOLINE QUINONE USING A BACTERIUM OF THE GENUS METHYLOBACTERIUM OR HYPHOMICROBIUM

AJINOMOTO CO., INC., Tok...

1. A method for producing pyrroloquinoline quinone (PQQ) comprising:
A) cultivating in a culture medium a bacterium belonging to the genus Hyphomicrobium, and

B) collecting PQQ from the culture medium,
wherein the bacterium has been modified to enhance expression of a pqqABCDE gene cluster from Hyphomicrobium dentirificans by a method selected from the group consisting of:

(i)increasing the copy number of the gene cluster,
(ii) introducing multiple copies of the gene cluster into the chromosome of said bacterium,
(iii) placing the gene cluster under the control of a potent promoter, and
(iv) combinations thereof.
US Pat. No. 9,187,570

FUSION PROTEIN

Ajinomoto Co., Ltd., Tok...

1. A fusion protein, comprising:
a DPS,
a first peptide portion capable of binding to a first target substance, and
a second peptide portion capable of binding to a second target substance,wherein
the DPS is a protein consisting of an amino acid sequence having 95% or more identity to an amino acid sequence represented
by SEQ ID NO: 4 or SEQ ID NO: 29,

the first peptide portion and the second peptide portion are each capable of binding to a different target substance, and
the first peptide portion and the second peptide portion are each independently capable of binding to a metal material, a
silicon material or a carbon material.

US Pat. No. 9,192,593

AMINO-ACID CONTAINING COMPOSITION FOR INHIBITING ACCUMULATION OF FAT

Ajinomoto Co., Inc., Tok...

1. A method for inhibiting accumulation of fat in fat tissue of a subject in need thereof, comprising administering to said
subject an effective amount of the amino-acid containing composition comprising leucine in an amount of 33 to 67% by weight
and threonine in an amount of 33 to 67% by weight, in terms of free amino acids thereof, based on whole amino acids, wherein
said subject is a mammal or a bird.

US Pat. No. 9,115,107

HETEROARYLCARBOXYLIC ACID ESTER DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. A method for the prophylaxis or treatment of type II diabetes or hyperglycemia, comprising administering an effective amount
of a compound represented by formula (I):

wherein
R1, R2, R3, and R4 may be the same or different and are each independently a hydrogen atom, a nitro group, a halogeno group,
a cyano group, a hydroxyl group, a thiol group, an amino group, a guanidino group, a formyl group, a lower alkyl group, a
lower alkenyl group, a lower alkynyl group, a lower acyl group, a carboxyl group, a sulfo group, a phosphono group, a lower
alkoxyl group, a lower alkylthio group, a lower alkylamino group, a lower acyloxy group, a lower acylamino group, a lower
alkoxycarbonyl group, a carbamoyl group, a lower alkylcarbamoyl group, a lower alkylsulfonylamino group, or a sulfamoyl group;

HetAr is a furan ring optionally having substituent(s);
X is a lower alkylene group optionally having substituent(s), a lower alkenylene group optionally having substituent(s), a
lower alkynylene group optionally having substituent(s), or a thiophenylene group;

Y is a carbonyl group, a thiocarbonyl group, or a sulfonyl group; and
A is a group of formula (II):

wherein R6 and R7 may be the same or different and are each independently a hydrogen atom, a hydroxyl group, a lower alkyl
group optionally having substituent(s), a lower alkenyl group optionally having substituent(s), a lower alkynyl group optionally
having substituent(s), or a lower alkoxyl group optionally having substituent(s), or R6 and R7 may be bonded to form a cyclic
amino group optionally having substituent(s),

or a pharmaceutically acceptable salt thereof,
to a subject in need thereof.

US Pat. No. 9,353,148

METHOD FOR PRODUCING PEPTIDE

AJINOMOTO CO., INC., Chu...

1. A method of producing a peptide, comprising:
(1) condensing an N-terminal amino group of (a) a C-protected amino acid or (b) a C-protected peptide, with a C-terminal carboxy
group of (c) an N-protected amino acid or (d) an N-protected peptide in a solvent to obtain a reaction solution which comprises
an (e) N-protected C-protected peptide,

wherein a C-terminal carboxy group of said (a) C-protected amino acid or said (b) C-protected peptide is protected by an anchor
group;

(2) removing the N-terminal protecting group of said (e) N-protected C-protected peptide in said reaction solution, without
isolating said N-protected C-protected peptide from said reaction solution, to obtain (b?) a C-protected peptide, wherein
when said condensing is conducted with said (b) C-protected peptide, then said (b) C-protected peptide and said (b?) C-protected
peptide are different; and

(3) precipitating said C-protected peptide in a solvent comprising a water-acetonitrile mixture, wherein said water-acetonitrile
mixture comprises 60 to 95% v/v acetonitrile, based on the total volume of said mixture, after said (2) removing; and

(4) obtaining said C-protected peptide by solid-liquid separation,
wherein said anchor group is a group represented by formula (II):
wherein:
R1 is a hydrogen atom or, when Rb is a group represented by the following formula (a), optionally forms a single bond together with R3 to form a fluorene ring together with ring A and ring B;

each R2 is independently an organic group having an aliphatic hydrocarbon group;

p is an integer of 1 to 4;
ring A optionally further has, in addition to OR2, a substituent selected from the group consisting of a halogen atom, a C1-6 alkyl group optionally substituted by a halogen atom, and a C1-6 alkoxy group optionally substituted by a halogen atom;

Ra is a hydrogen atom, or a phenyl group optionally substituted by a halogen atom; and

Rb is a hydrogen atom, or a group represented by formula (a):


wherein:
* indicates the position of binding to the remainder of the molecule;
r is an integer of 0 to 4;
each R4 is independently an organic group having an aliphatic hydrocarbon group;

R3 is a hydrogen atom, or optionally forms a single bond together with R1 to form a fluorene ring together with ring A and ring B; and

ring B optionally further has, in addition to OR4, a substituent selected from the group consisting of a halogen atom, a C1-6 alkyl group optionally substituted by a halogen atom, and a C1-6 alkoxy group optionally substituted by a halogen atom; and

L represent a connection to the carbonyl group of said C-terminus and is —O— or —N(R)—, wherein R is a hydrogen atom, an alkyl
group, or an aralkyl group.

US Pat. No. 9,340,795

GENETICALLY MODIFIED PLANT CAPABLE OF BIOSYNTHESIZING CAPSINOID

AJINOMOTO CO., INC., Tok...

1. A method of producing a genetically modified plant having an increased capability to produce capsinoids, comprising:
(a) introducing a DNA encoding an antisense RNA against putative aminotransferase (pAMT) mRNA into a plant that is a pungent
variety belonging to the genus Capsicum and produces capsaicinoids in its fruit to produce a genetically modified plant,

wherein the antisense RNA against pAMT mRNA is complementary to an mRNA of the endogenous pAMT gene of the plant, and has
a nucleotide sequence having 95% or more identity to the nucleotide sequence of SEQ ID NO:1 from Capsicum annuum CH-19 Hot,

(b) measuring the content of capsinoids in a fruit of the genetically modified plant from (a), and
(c) selecting the genetically modified plant from (b) having an increased amount of capsinoids production as compared to that
of the corresponding non-genetically modified plant.

US Pat. No. 9,334,511

METHOD FOR PRODUCING BASIC SUBSTANCE

Ajinomoto Co., Inc., Tok...

1. A method for producing a basic amino acid by fermentation comprising culturing a microorganism having an ability to produce
the basic amino acid in a liquid medium contained in a fermentation tank to produce and accumulate the basic amino acid in
the medium;
wherein the amount of sulfate and/or chloride ions used as counter ions of the basic amino acid is reduced by adjusting the
total ammonia concentration in the medium to be 300 mM or lower during at least a part of the total period of the culture
process including a period where the pH of the medium increases due to shortage of the counter ions caused by accumulation
of the objective basic amino acid;

wherein the total ammonia concentration in the medium is adjusted by adding ammonia or urea to the medium when an activity
of the microorganism is reduced or ceases as determined based on the indicators: dissolved oxygen concentration in the medium,
consumption rate of carbon source in the medium, turbidity of the medium, productivity of the basic amino acid, and pH change
in the medium; and

wherein the microorganism is an Escherichia coli bacterium or a coryneform bacterium.

US Pat. No. 9,181,202

CRYSTALS OF SALTS OF PHENYLALANINE DERIVATIVES

AJINOMOTO CO., INC., Chu...

1. A crystal of a pharmaceutically acceptable acid-addition salt of the compound represented by the following formula (I):

US Pat. No. 9,127,039

SULFUR-CONTAINING AMINO ACID DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. An antibody which specifically recognizes the compound represented by the following formula (I):
wherein X is an immunoresponsive hydrophobic group selected from the group consisting of 9-fluorenylmethyloxycarbonyl, quinolinylaminocarbonyl,
4-N,N-dimethylaminosulfonyl-7-piperazino-2,1,3-benzoxadiazole, 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole, 5-N,N-dimethylaminonaphthalenesulfonyl
chloride, o-phthalaldehyde, 4-phenylspiro[furan-2(3H),1?-phthalan]-3,3?-dione, N,N,N-trimethylammonioanilyl N?-hydroxysuccinimidyl
carbamate iodide, and fluorescein , Y is a group bound to an endogenous low-molecular-weight compound selected from the group
consisting of an amino acid, an organic acid, a sugar, a sugar phosphate, a nucleic acid, a free fatty acid, and an oligopeptide,
Z is selected from the group consisting of a hydrogen atom, a high-molecular-weight-imparting group, and a labeling compound
modifying group, and A is a single bond or a C1-6alkylene group, wherein the antibody recognizes at least the immunoresponsive hydrophobic group and the endogenous low-molecular-weight
compound in the compound of Formula I,
wherein said endogenous low-molecular-weight compound has a molecular weight of 1000 or below.
US Pat. No. 9,395,376

KOKUMI-IMPARTING AGENT

AJINOMOTO CO., INC., Tok...

1. A method for preparing a food or beverage that contains a kokumi-imparting substance, the method comprising:
A) a first step of detecting the calcium receptor activating ability of a test substance, which is an indication of the ability
to impart kokumi, by using recombinant cells which have been transformed with a foreign calcium receptor gene, wherein the
test substance enhances a taste selected from the group consisting of salty taste, umami taste, sweet taste, sour taste, and
combinations thereof;

B) a second step of measuring the kokumi-imparting effect of the test substance for which calcium receptor activating ability
has been detected in the first step;

C) selecting a substance which is effective to both activate the calcium receptor and impart kokumi, and
D) combining the substance selected in step C) with a food/beverage, thereby producing said food or beverage that contains
a kokumi-imparting substance.

US Pat. No. 9,169,187

METHOD OF MAKING PEPTIDES USING DIPHENYLMETHANE COMPOUND

AJINOMOTO CO., INC., Tok...

1. A method of producing a peptide, comprising:
(a) condensing a diphenylmethane compound represented by formula (I)
wherein
Y is a hydroxyl group or a —NHR group, where R is a hydrogen atom, an alkyl group, or an aralkyl group;
k and l are each independently an integer of 0 to 5 and k+l is not 0;
each Ra in the number of k and each Rb in the number of l are independently an organic group having an aliphatic hydrocarbon group, wherein, in the organic group(s)
in the number of (k+l), each having an aliphatic hydrocarbon group, the total carbon number of the aliphatic hydrocarbon groups
is not less than 16;

ring A optionally further has substituent(s) besides Ra; and

ring B optionally further has substituent(s) besides Rb;

with a —CONHR group of an N-protected amino acid or N-protected peptide having a —CONHR group, wherein R is a hydrogen atom,
an alkyl group or an aralkyl group, or

with a —COOH group of an N-protected amino acid or an N-protected peptide having a —COOH group to produce a C-diphenylmethane-protected
amino acid or a C-diphenylmethane-protected peptide;

(b) deprotecting the N-terminal of the C-diphenylmethane-protected amino acid or C-diphenylmethane-protected peptide, to produce
an N-unprotected amino acid or N-unprotected peptide;

(c) condensing the N-terminal of the N-unprotected amino acid or N-un-protected peptide with an N-protected amino acid or
N-protected peptide, to obtain an elongated peptide; and

(d) isolating the elongated peptide.
US Pat. No. 9,187,554

METHOD FOR SECRETORY PRODUCTION OF PROTEIN

AJINOMOTO CO., INC., Tok...

1. A coryneform bacterium having an ability to produce a heterologous protein by secretory production, wherein said bacterium
is modified to have reduced activities of a penicillin-binding protein and a cell surface layer protein, and
wherein the penicillin-binding protein is a PBP1 a protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence of SEQ ID NO: 82,
(B) a protein comprising an amino acid sequence of SEQ ID NO: 82, but which includes substitution, deletion, insertion, or
addition of 1 to 10 amino acid residues, and wherein said protein has a property that if the protein activity is reduced in
the coryneform bacterium, the amount of the heterologous protein produced by secretory production is increased compared with
that observed for a non-modified strain; and

wherein the cell surface layer protein is a CspB protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence of SEQ ID NO: 98,
(B) a protein comprising an amino acid sequence of SEQ ID NO: 98, but includes substitution, deletion, insertion, or addition
of 1 to 10 amino acid residues, and wherein said protein has a property that if the protein activity is reduced in the coryneform
bacterium, the amount of the heterologous protein produced by secretory production is increased compared with that observed
for a non-modified strain.

US Pat. No. 9,464,167

POLY ?-AMINO ACID AND FERROELECTRIC MEMORY ELEMENT USING SAME

AJINOMOTO CO., INC., Chu...

1. A ferroelectric memory element, comprising a ferroelectric layer, wherein said ferroelectric layer comprises a poly ?-amino
acid which is a copolymer comprising:
(i) a glutamic acid-?-ester unit represented by formula (I):

wherein R1 is a substituted or unsubstituted alkyl group having 1 to 8 carbon atoms, or a benzyl group optionally substituted by a halogen
atom, an alkoxy group or a nitro group; and either

(iia) a glutamic acid-?-ester unit represented by formula (II):

wherein R2 is an unsubstituted alkyl group having 3 to 16 carbon atoms, or an alkyl group having 1 to 6 carbon wherein a part or all
of the hydrogen atoms are substituted by a halogen atom, an alicyclic hydrocarbon group having 3 to 12 carbon atoms, an alkoxy
group having 1 to 6 carbon atoms, a cyano group, a phenyl group in which a part or all of the hydrogen atoms are optionally
substituted by a halogen atom or an alkoxy group, a phenyl alkoxy group in which a part or all of the hydrogen atoms are optionally
substituted by a halogen atom or an alkoxy group, or a phenylalkyl carbamate group in which a part or all of the hydrogen
atoms are optionally substituted by a halogen atom or an alkoxy group, provided that R2 is not the same as R1; or

(iib) an alanine, phenylalanine or N-substituted lysine unit represented by formula (III):

wherein R3 is a methyl group, a benzyl group or a —(CH2)4—NHX group (wherein X is a benzyloxycarbonyl group optionally substituted by a halogen atom or an alkoxy group, an alkylcarbonyl
group optionally substituted by a halogen atom, an allyloxycarbonyl group, a fluorenylalkoxycarbonyl group, a benzenesulfonyl
group optionally substituted by an alkyl group or a nitro group, or an alkyloxycarbonyl group),

wherein the molar ratio of units of formula (I) to units of formula (II) or units of formula (III), (I)/(II) or (I)/(III),
is 10/90 to 90/10.

US Pat. No. 9,173,420

FEED ADDITIVE COMPOSITION FOR RUMINANTS AND METHOD OF PRODUCING THE SAME

Ajinomoto Co., Inc., Tok...

1. A feed additive composition, comprising:
39.5 to 59.5% by weight of at least one protective agent selected from the group consisting of a hardened vegetable oil having
a melting point higher than 50° C. and lower than 90° C. and a hardened animal oil having a melting point higher than 50°
C. and lower than 90° C.;

0.05 to 5% by weight of lecithin;
40% to 60% by weight of at least one of L-lysine and a salt of L-lysine; and
2 to 6% by weight water;
wherein the feed additive composition is a dispersion-type preparation having water repellency on a surface thereof.
US Pat. No. 9,080,189

METHOD FOR PRODUCING AN ORGANIC ACID

AJINOMOTO CO., INC., Tok...

1. A method for producing an organic acid comprising:
A) allowing a substance to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions,
or carbon dioxide gas, wherein the substance is selected from the group consisting of:

i) a bacterium which has an ability to produce an organic acid and has been modified to have enhanced expression of the sucE1
and mdh genes,

ii) a product obtained by processing the bacterium of i), and
iii) combinations thereof, and
B) collecting the organic acid,wherein the sucE1 gene is selected from the group consisting of:
(a) a DNA comprising the nucleotide sequence of numbers 571 to 2187 of SEQ ID NO: 3, or the nucleotide sequence of SEQ ID
NOs: 5, 7 or 11,

(b) a DNA which hybridizes with a nucleotide sequence complementary to the nucleotide sequence of the numbers 571 to 2187
of SEQ IS NO: 3, or the nucleotide sequence of SEQ ID NOs: 5, 7 or 11, under stringent conditions comprising washing at 0.1x
SSC, 0.1% SDS at 68° C., and wherein the DNA improves the ability of the bacterium to produce succinic acid when expression
of the DNA is enhanced in the bacterium,

(c) a DNA which encodes a protein having the amino acid sequence of SEQ ID NOs: 4, 6, 8, or 12, and
(d) a DNA which encodes a protein having a homology of not less than 95% to the entire amino acid sequence of SEQ ID NOs:
4, 6, 8, or 12, and wherein said protein improves the ability of the bacterium to produce succinic acid when expression of
the DNA is enhanced in the bacterium,

wherein the mdh gene is selected from the group consisting of:
(A) a DNA comprising the nucleotide sequence of the numbers 301 to 1287 of SEQ ID NO: 13,
(B) a DNA which hybridizes with a nucleotide sequence complementary to the nucleotide sequence of the numbers 301 to 1287
of SEQ ID NO: 13 under stringent conditions comprising washing at 0.1x SSC, 0.1% SDS at 68° C., and codes for a protein having
malate dehydrogenase activity,

(C) a DNA encoding a protein having the amino acid sequence of SEQ ID NO: 14, and
(D) a DNA encoding a protein having a homology not less than 95% to the entire amino acid sequence of SEQ ID NO: 14, and wherein
said protein has malate dehydrogenase activity, and

wherein enhanced expression is obtained by a method selected from the group consisting of
i) increasing the copy number of the sucE1 gene and/or the mdh gene,
ii) modifying an expression control sequence of the sucE1 gene and/or the mdh gene,
iii) replacing a promoter of the sucE1 gene and/or the mdh gene with a stronger promoter, and
iv) combinations thereof.

US Pat. No. 9,456,971

MOISTURIZER AND COSMETIC AGENT CONTAINING SAME

AJINOMOTO CO., INC., Tok...

1. A moisturizer comprising an acylproline of formula (1) or a salt thereof

wherein an acyl group represented by R1—CO— is an acyl group of a saturated or unsaturated fatty acid having 6 to 14 carbon atoms.

US Pat. No. 9,458,288

POLYESTER COMPOUND

Ajinomoto Co., Inc., Tok...

1. A polyester compound, which is produced by reacting:
(1) a compound represented by formula (1):
wherein
R1 is a hydroxy group, a halogen atom, an alkoxy group, a cycloalkyloxy group, an aryloxy group, a —OM, or a group —O—Si(R2)3, where M is a metal atom and R2 is an alkyl group;

XDc is a phenylene group optionally having a substituent, a naphthylene group optionally having a substituent, an anthracenylene
group optionally having a substituent, a furandiyl group optionally having a substituent, a pyridinediyl group optionally
having a substituent, a thiophenediyl group optionally having a substituent, or a quinolinediyl group optionally having a
substituent;

YDc is —O—, —N?N—, a carbonyl group, an ethenylene group optionally having a substituent, or a single bond;

nDc is an integer of 0 to 2;

the two R1 may be the same as or different from each other;

when there are a plurality of XDc, they may be the same as or different from each other; and

when there are a plurality of YDc, they may be the same as or different from each other,

with
(2) a compound represented by formula (2):
wherein
R4 is a hydrogen atom, an acyl group, or a group —Si(R5)3, where R5 is an alkyl group;

X is a phenylene group optionally having a substituent selected from the group consisting of a halogen atom and an alkyl group,
or a naphthylene group optionally having a substituent selected from the group consisting of a halogen atom and an alkyl group;

Y is a methylene group optionally having a substituent, a group —S(?O)2—, or a single bond;

Z is a divalent aromatic group optionally having a substituent;
n is 0 or 1; and
the two R4 may be the same as or different from each other.

US Pat. No. 9,428,783

DNA ENCODING DIPEPTIDE-SYNTHESIZING ENZYME (VARIANTS), BACTERIUM BELONGING TO THE GENUS ESCHERICHIA, AND METHODS FOR PRODUCING DIPEPTIDES USING THEREOF

Ajinomoto Co., Inc., Tok...

1. A method for producing a dipeptide or a salt thereof, comprising:
(a) reacting L-amino acids, L-amino acid derivatives, or salts thereof under appropriate conditions in the presence of a protein
having dipeptide-synthesizing activity such that a dipeptide or a salt thereof is produced;

(b) accumulating the dipeptide or a salt thereof in an appropriate solvent; and
(c) collecting the dipeptide or a salt thereof from the appropriate solvent,
wherein the protein is selected from the group consisting of:
a protein having the amino acid sequence of SEQ ID NO: 2, 4, or 6;
a variant of a protein having the amino acid sequence of SEQ ID NO: 2, 4, or 6 in which no more than fifteen amino acid residues
of the amino acid sequence of SEQ ID NO: 2, 4, or 6 are changed by substitution, deletion, insertion, or addition, and wherein
the variant protein has the dipeptide-synthesizing activity; and

a protein having an amino acid sequence having not less than 95% sequence identity to the amino acid sequence of SEQ ID NO:
2, 4, or 6 and wherein the protein has the dipeptide-synthesizing activity,

wherein the dipeptide comprises two L-amino acids, two L-amino acid derivatives, or an L-amino acid and an L-amino acid derivative,
and

wherein the L-amino acids or the L-amino acid derivatives are selected from the group consisting of L-alanine, L-arginine,
L-asparagine, L-aspartic acid, L-cysteine, L-glutamic acid, L-glutamine, glycine, L-histidine, L-isoleucine, L-leucine, L-lysine,
L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine, L-valine, and a lower alkyl ester
of L-phenylalanine, wherein the lower alkyl ester of L-phenylalanine is methyl, ethyl or propyl ester of the L-phenylalanine.

US Pat. No. 9,200,304

METHOD FOR PRODUCING 5?-GUANYLIC ACID

AJINOMOTO CO., INC., Tok...

1. A method for producing 5?-guanylic acid comprising:
A) reacting a microorganism with xanthylic acid to produce 5?-guanylic acid, and
B) collecting 5?-guanylic acid;wherein said microorganism is able to convert xanthylic acid into 5?-guanylic acid, and has been modified so that:
a) a nagD gene does not function normally, and
b) 5?-guanylic acid synthetase activity is enhanced as compared to a non-modified microorganism by increasing expression of
a guaA gene in said microorganism,
wherein said guaA gene encodes a protein comprising an amino acid sequence having an identity of not less than 95% to the
entire amino acid sequence of SEQ ID NO: 2, 4, 6, or 8, andwherein said protein has 5?-guanylic acid synthetase activity, and wherein said microorganism is bacteria selected from the
group consisting of Enterobacteriaceae bacteria, Bacillus bacteria, and coryneform bacteria.

US Pat. No. 9,174,932

CASR AGONISTS

AJINOMOTO CO., INC., Tok...

1. A compound of formula (I) or a pharmaceutically acceptable salt thereof:

wherein R1, R3, and R5 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxyl
group, —NH2, an optionally substituted alkyl group having 1 to 6 carbon atoms, and an optionally substituted alkoxy group having 1 to
6 carbon atoms;

R4 is selected from the group consisting of a halogen atom, a hydroxyl group, —NH2, and an optionally substituted alkoxy group having 1 to 6 carbon atoms;

R2 is selected from the group consisting of a sulfonic acid group,

R6 and R7 are each independently a hydrogen atom or an optionally substituted alkyl group having 1 to 6 carbon atoms; and

X is a methylene group or an oxygen atom;
and excluding
a compound wherein X is a methylene group, and R2 is

US Pat. No. 9,211,245

COSMETIC COMPOSITION

AJINOMOTO CO., INC., Tok...

1. A cosmetic composition, comprising:
(A) at least one acyl proline represented by formula (I):

wherein R1—CO— is an acyl group derived from a saturated or unsaturated fatty acid having 4 to 18 carbon atoms, or a salt thereof; and

(B) one or more compounds selected from the group consisting of anisic acid, phenylmethanol, phenethyl alcohol and phenylpropanol,
wherein the content of (A) based on the total weight of the composition is from 0.01 wt % to 5 wt %, and the content of (B)
based on the total weight of the composition is from 0.05 wt %-1 wt %.

US Pat. No. 9,464,110

ACYL DIPEPTIDE DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. An acyl dipeptide compound represented by formula (1):

wherein:
R1—CO— is a decanoyl group; and

R2, R3 and R4 are each a hydrogen atom,

or a salt thereof.
US Pat. No. 9,462,810

METHOD FOR INACTIVATING VIRUSES WITH SLIGHTLY ACIDIC ARGININE

AJINOMOTO CO., INC., Tok...

1. A method for producing a protein formulation in which lipid-enveloped viruses are inactivated, comprising:
A) preparing an aqueous solution containing 0.1 to 2 M of arginine, an arginine derivative, or a mixture thereof, and
B) exposing a protein formulation contaminated with one or more lipid-enveloped viruses to an amount of the aqueous solution
sufficient to inactivate the lipid-enveloped viruses, wherein the pH of the resultant solution is in the range of between
4 and 5.

US Pat. No. 9,216,554

PROCESS OF FUSION-BONDING PLASTIC FILM AND DRUG BAG

AJINOMOTO CO., INC., Chu...

1. A process of manufacturing a drug bag, comprising:
sealing a front-side plastic film and a back-side plastic film such that a drug bag body having a plurality of compartments
is formed, the plurality of compartments comprising a first compartment and a second compartment separated by a weak seal
portion;

placing a portion of a port member between the front-side plastic film and the back-side plastic film;
placing a plastic film for a handle on a front surface of the front-side plastic film;
disposing a heat generation element on a front surface of the plastic film for the handle;
pressing a press member toward the heat generation element, the plastic film for the handle and the front-side plastic film;
irradiating infrared laser to the heat generation element through the press member during the pressing such that the heat
generation element generates heat by absorbing infrared laser transmitted through the press member; and

fusion-bonding the plastic film for the handle of the front-side plastic film,
wherein the port member is placed such that one of the compartments communicates with outside of the drug bag body through
the port member, the weak seal portion is unsealable by pulling the plastic film for the handle away from the front-side plastic
film of the drug bag body, the weak seal portion includes a first weak seal portion near the port member and a second weak
seal portion far from the port member, and

the heat generation element is disposed at equal distances from the first weak seal portion and the second weak seal portion.
US Pat. No. 9,447,443

METHOD FOR PRODUCING 3-ACETYLAMINO-4-HYDROXYBENZOIC ACID

AJINOMOTO CO., INC., Tok...

1. A method for producing 3-acetylamino-4-hydroxybenzoic acid or a salt thereof, comprising:
a) culturing a microorganism so that 3-acetylamino-4-hydroxybenzoic acid or a salt thereof is produced, and
b) collecting said 3-acetylamino-4-hydroxybenzoic acid or salt thereof;
wherein the microorganism is transformed with a recombinant vector encoding a GriI protein, a GriH protein, or GriI and GriH
proteins, and has increased formation of 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde
as compared to the same microorganism that does not comprise said recombinant vector, and

wherein the microorganism is transformed with a recombinant vector encoding a N-hydroxyarylamine O-acetyltransferase (NhoA)
protein and has increased NhoA activity as compared to the same microorganism that does not comprise said recombinant vector,
and wherein said NhoA protein is selected from the group consisting of:

(I) a protein comprising the amino acid sequence of SEQ ID NO: 2;
(II) a protein comprising the amino acid sequence of SEQ ID NO: 2, but having no more than one to ten amino acid substitutions,
deletions, insertions or additions, and wherein said protein has NhoA activity;

(III) a protein comprising an amino acid sequence having 95% or more sequence identity to the amino acid sequence of SEQ ID
NO:2 and having NhoA activity; and

(IV) combinations thereof.

US Pat. No. 9,458,289

POLYESTER COMPOUND

Ajinomoto Co., Inc., Tok...

1. A polyester compound produced by reacting:
(1) a compound represented by formula (1):
wherein
R1 is a hydroxy group, a halogen atom, an alkoxy group, a cycloalkyloxy group, an aryloxy group, a group ˜OM, or a group —O—Si(R2)3, where M is a metal atom and R2 is an alkyl group;

XDc is a divalent aromatic group optionally having a substituent;

YDc is —O—, —N?N—, a carbonyl group, an alkenylene group optionally having a substituent, or a single bond;

nDc is an integer of 0 to 2;

the two R1 may be the same as or different from each other;

when there are a plurality of XDc, they may be the same as or different from each other; and

when there are a plurality of YDc, they may be the same as or different from each other, with

(2) a compound represented by formula (2):
wherein
R4 is a hydrogen atom, an acyl group, or a group —Si(R5)3, where R5 is an alkyl group;

X is a divalent aliphatic hydrocarbon group optionally having a substituent;
Y is an imino group optionally having a substituent, a divalent aromatic group optionally having a substituent, an oxyalkylene
group optionally having a substituent, a group —S(?O)2—, or an alkylene group optionally having a substituent;

Z is a divalent aliphatic hydrocarbon group optionally having a substituent or a single bond;
n is an integer of 0 to 5;
the two R4 may be the same as or different from each other;

when there are a plurality of Y, they may be the same as or different from each other; and
when there are a plurality of Z, they may be the same as or different from each other.

US Pat. No. 9,271,521

INHIBITOR FOR LIVER CANCER ONSET AND PROGRESS

AJINOMOTO CO., INC., Tok...

1. A method for treating liver cancer in a subject in need thereof, which comprises administering an effective amount of a
therapeutic composition to said subject in need thereof,
wherein said therapeutic composition consists of isoleucine, leucine, valine, one or more pharmacologically acceptable carrier,
and, optionally, at least one additional drug for treating liver disease selected from the group consisting of an interferon,
glycyrrhizin, ursodeoxycholic acid, Ribavirin, and Chinese medicine sho-saiko-to,

wherein said isoleucine, leucine, and valine are administered in a weight ratio of 1:1.9 to 2.2:1.1 to 1.3,
wherein said subject is a patient with liver cirrhosis,
wherein said pharmacologically acceptable carrier is one or more excipient, binder, lubricant, solvent, disintegrant, dissolution
aid, suspending agent, emulsifier, isotonicity agent, stabilizer, soothing agent, preservative, antioxidant, corrigent, coloring
agent, or a mixture thereof,

wherein said excipient is one or more excipient selected from the group consisting of a saccharide, a starch, a cellulose,
calcium carbonate, and kaolin,

wherein said stabilizer is selected from the group consisting of polyethylene glycol, and sodium dextran sulfate, and
wherein said antioxidant is selected from the group consisting of subsulfate and ascorbic acid.
US Pat. No. 9,265,708

LIQUID CLEANSER COMPRISING STEROL ESTER AND C5-6 HYDROXYALCOHOL

AJINOMOTO CO., INC., Tok...

1. A composition, comprising:
(A) at least one sterol ester;
(B) one or more anionic surfactants selected from (B1) N-acylamino acid having a C8-22 acyl group or a salt thereof, and (B2) polyoxyethylene alkyl ether sulfate or a salt thereof;

(C) at least one betaine-type amphoteric surfactant;
(D) at least one glyceryl mono-C8-14 fatty acid ester;

(E) at least one C5-6 hydroxyalcohol; and

(F) water, andhaving a pH of 4.0 to 8.0.
US Pat. No. 9,301,937

AMINO-ACID-CONTAINING MEDICINAL GRANULAR PREPARATION HIGHLY EASY TO TAKE

AJINOMOTO CO., INC., Tok...

1. A granule preparation comprising:
granules comprising an amino acid comprising isoleucine, leucine and valine, wherein the granules have a particle size distribution
such that an amount of granules on an aperture 355 ?m sieve is 100% and an amount of granules on an aperture 710 ?m sieve
is 0%, and the granules have a bulk density of not less than 0.59 g/mL and not more than 0.75 g/mL,

wherein a weight ratio of isoleucine, leucine and valine is 1:1.5-2.5:0.8-1.7.

US Pat. No. 9,253,997

ALKYLAMINE DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. A compound of Formula (I) or a salt thereof:

wherein R1 and R2, each independently, represent a hydrogen atom, or unsubstituted C1-6 alkyl;

R3 represents a hydrogen atom;

R4 and R5, each independently, represent a hydrogen atom, substituted or unsubstituted C1-6 alkyl;

X represents CH2, an oxygen atom, NH, or a sulfur atom;

Y represents C?O, SO, SO2, or C?S;

R6 represents a hydrogen atom;

G represents R7-substituted phenyl or R7-substituted pyridyl, where the R7-substituted phenyl or the R7-substituted pyridyl may further be substituted with one or two R8;

R7 represents sulfo or carboxyl;

R8 represents substituted or unsubstituted C1-6 alkyl, halogeno, hydroxy, substituted or unsubstituted C1-6 alkoxy, sulfo, or C1-3 alkylcarbonylamino, where they may be different when more than one R8 exist;

Q represents a hydrogen atom, substituted or unsubstituted C1-6 alkyl, carboxyl, CONReRf, CONHNHRg, CORh, phenyl, or substituted or unsubstituted oxadiazolyl;

Re and Rf, each independently, represent a hydrogen atom, substituted or unsubstituted C1-6 alkyl, substituted or unsubstituted C1-6 alkylsulfonyl, phenylsulfonyl, substituted or unsubstituted C3-8 cycloalkyl, or hydroxy, or alternatively, Re and Rf may integrally form morpholino;

Rg represents substituted or unsubstituted C1-6 alkylcarbonyl; and

Rh represents substituted or unsubstituted C1-6 alkoxy,

provided that when X is methylene or an oxygen atom, Y is C?O, all of R1-R5 are hydrogen atoms, and G is R7-substituted phenyl optionally substituted with one or two R8, then, Q is a group other than carboxyl or CORh.

US Pat. No. 9,192,624

MEDICAL MATERIAL AND METHOD FOR MANUFACTURING SAME

AJINOMOTO CO., INC., Tok...

1. A method for promoting mesenchymal stem cell differentiation, which comprises:
(a) cultivating a mesenchymal stem cell in the presence of a medical material comprising a polyamino acid, or
(b) transplanting a medical material comprising a polyamino acid to a subject in need thereof,
wherein the medical material has a cell adhesion suppressive effect, and
wherein the polyamino acid consists of:
(i) one kind of amino acid residue selected from the group consisting of an alanine residue, a valine residue, a leucine residue,
a phenylalanine residue, a glycine residue, a glutamine residue, an aspartic acid residue containing a protecting group in
the side chain, a tyrosine residue optionally containing a protecting group in the side chain, a tryptophan residue optionally
containing a protecting group in the side chain, a lysine residue containing a protecting group in the side chain, and a glutamic
acid residue containing a protecting group in the side chain; or

(ii) two kinds of amino acid residues selected from the group consisting of an alanine residue, a valine residue, a leucine
residue, an isoleucine residue, a phenylalanine residue, a lysine residue optionally containing a protecting group in the
side chain, and a glutamic acid residue optionally containing a protecting group in the side chain,

excluding polyamino acids consisting of two kinds of amino acid residues of (1) glutamic acid and alanine and (2) lysine and
phenylalanine;

wherein the medical material is a fiber structure,
wherein the polyamino acid is:
poly-L-alanine;
poly-L-valine;
poly-N?-benzyloxycarbonyl-L-lysine;

poly-L-alanine/?-methyl-L-glutamic acid;
poly-L-leucine/?-methyl-L-glutamic acid;
poly-L-alanine/N?-benzyloxycarbonyl-L-lysine;

poly-L-leucine;
poly-L-valine/?-methyl-L-glutamic acid;
poly-L-isoleucine/?-methyl-L-glutamic acid;
poly-L-phenylalanine/?-methyl-L-glutamic acid;
poly-L-valine/N?-benzyloxycarbonyl-L-lysine;

poly-L-isoleucine/N?-benzyloxycarbonyl-L-lysine; or

poly-L-valine/L-phenylalanine.

US Pat. No. 9,226,884

LIQUID CLEANSING COMPOSITION

AJINOMOTO CO., INC., Chu...

1. A liquid cleansing composition, comprising
(A) at least one compound represented by formula (1):
wherein R1 is a saturated or unsaturated, linear or branched-chain hydrocarbon group having a carbon number of 11 to 25, and R2 and R3 are each independently a hydrogen atom or an alkyl group having a carbon number of 1 to 6, or a salt thereof; and
(B) at least one sugar type surfactant and/or at least one sulfate type surfactant.

US Pat. No. 9,175,028

GLYCOSIDE COMPOUND

AJINOMOTO CO., INC., Chu...

1. A compound represented by formula (I?):
wherein:
R11, R12, R13, R14 and R15 are each independently a hydrogen atom, a hydroxyl group, a C1-6 alkyl group, a C1-6 alkoxy group, a C1-6 alkyl-carbonyloxy group or a G-O— group, and at least one of R11, R12, R13, R14 and R15 is a G-O— group, wherein G is a saccharide residue selected from the group consisting of a glucosyl group, a rhamnosyl group,
a cellobiosyl group, a maltosyl group, and a maltotriosyl group;

X1 is a methylene group, an ethylene group, a trimethylene group, a vinylene group or —CH?CH—CH2—;

X2 is —CO—O— or —O—CO—;

p and q are each an integer of 0 to 7, which satisfy a relationship of p+q=2 to 8;
Y1 is an ethylene group or an alkenylene group having a carbon number of 2 to 15 and 1 to 3 double bonds, wherein the double
bond may be any of cis and trans; and

R16 and R17 are each independently a hydrogen atom, a methyl group or an ethyl group, or R16 and R17 together with the carbon atom to which they are bonded form a C3-6 cycloalkane group.

US Pat. No. 9,228,215

METHOD FOR PRODUCING A TARGET SUBSTANCE BY FERMENTATION

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid utilizing a microorganism, which comprises:
A) culturing the microorganism in a medium, and
B) collecting the L-amino acid from the medium,
wherein the microorganism has been modified so that:
A) it comprises isomaltase activity,
B) the isomaltase activity is increased, or
C) both
wherein said activity causes the decomposition of phosphorylated isomaltose into glucose and glucose phosphate;
wherein the isomaltase is encoded by a DNA selected from the group consisting of:
(A) a DNA comprising the nucleotide sequence shown in SEQ ID NO: 39,
(B) a DNA which is able to hybridize with a sequence complementary to the nucleotide sequence of SEQ ID NO: 39 under stringent
conditions comprising washing at a salt concentration and temperature of 0.1×SSC, 0.1% SDS at 60° C., and codes for a protein
having isomaltase activity, and

wherein the microorganism comprises a gene coding for PTS maltose enzyme IICB,
wherein the PTS maltose enzyme IICB is encoded by a DNA selected from the group consisting of:
(A) a DNA comprising the nucleotide sequence shown in SEQ ID NO: 41,
(B) a DNA which is able to hybridize with a sequence complementary to the nucleotide sequence of SEQ ID NO: 41 under stringent
conditions comprising washing at a salt concentration and temperature of 0.1×SSC, 0.1% SDS at 60° C., and codes for a protein
which is able to cause the microorganism to take up isomaltose, wherein the medium contains a component selected from the
group consisting of:

A) isomaltose, and
B) isomaltose and maltose.
US Pat. No. 9,334,512

METHOD FOR PRODUCING BASIC SUBSTANCE

Ajinomoto Co., Inc., Tok...

1. A method for producing a basic amino acid by fermentation comprising culturing a microorganism having an ability to produce
the basic amino acid in a liquid medium contained in a fermentation tank to produce and accumulate the basic amino acid in
the medium;
wherein the amount of sulfate and/or chloride ions used as counter ions of the basic amino acid is reduced by adjusting the
total ammonia concentration in the medium to be 300 mM or lower during at least a part of the total period of the culture
process including a period where the pH of the medium increases due to shortage of the counter ions caused by accumulation
of the objective basic amino acid;

wherein the total ammonia concentration in the medium is adjusted so to ensure growth of the microorganism or the production
of the basic amino acid, and at said concentration does not inhibit the production of the basic amino acid by the microorganism;
and

wherein the microorganism is an Escherichia coli bacterium or a coryneform bacterium.

US Pat. No. 9,173,407

DISEASE RESISTANCE ENHANCER FOR PLANTS AND METHOD OF CONTROLLING PLANT DISEASE BY USING THE SAME

AJINOMOTO CO., INC., Tok...

1. A method comprising:
applying to a plant a composition comprising a microbial cell extract so that pathogenic infectious diseases of said plant
are controlled,

wherein said microbial cell extract has been subjected to a heat treatment temperature of 70° C. or higher in an acidic solution,
wherein said acidic solution is at pH of 1 to 6, and
wherein said microbial cell is selected from the group consisting of Corynebacterium glutamicum, Pantoea ananatis, Bacillus subtilis, and Saccharomyces cerevisiae.

US Pat. No. 9,274,123

METHOD FOR ANALYSIS OF COMPOUNDS WITH AMINO GROUP AND ANALYTICAL REAGENT THEREFOR

AJINOMOTO CO., INC., Tok...

1. A method for analyzing a compound having an amino group, an imino group, or mixture thereof, in a sample by tandem mass
spectrometry, comprising:
(a) labeling the compound having an amino group, an imino group, or mixture thereof with a carbamate compound represented
by formula (Ia) or a salt thereof to form a labelled compound having a bond between a nitrogen of the amino group, imino group,
or mixture thereof and a labeling a portion of the carbamate compound represented by formula (Ia) or a salt thereof:


wherein Ar is an aromatic carbocyclic group or an aromatic heterocyclic group, wherein the aromatic carbocyclic group or the
aromatic heterocyclic group may have one or more substituents,

(b) selecting or scanning, in a first mass analyzer in positive ion mode, m/z values derived from pre-selected ions of the
labeled compound or ranges of m/z values which include ions of the labeled compounds;

(c) collisionally cleaving the ions of the labeled compound at the bond between the nitrogen of the amino group or the imino
group of the labelled compound and the labeling portion of the carbamate compound represented by formula (Ia) or a salt thereof
to produce a fragment ion and a neutral fragment; and

(d) selecting or scanning, in a second mass analyzer:
(1) pre-selected m/z values of a fragment ion derived from the labeling portion of the carbamate compound represented by formula
(Ia) or a salt thereof,

(2) pre-selected m/z values of a fragment ion derived from the compound having an amino group, an imino group, or a mixture
thereof, or

(3) ranges of m/z values at a pre-selected offset value from the first mass analyzer, wherein the offset is derived from loss
of the labeling portion of the carbamate compound represented by formula (Ia) or a salt thereof.

US Pat. No. 9,222,944

METHOD FOR SCREENING A SALTY TASTE MODULATING SUBSTANCE

AJINOMOTO CO., INC., Tok...

1. A method of screening for a salty taste modulating substance, which comprises the step of contacting a test substance with
a cell that expresses a Kv3.2 protein, and comparing observed cation influx into the cell with cation influx into the cell
observed when the test substance is not contacted with the cell, wherein
(a) when the cation influx is larger when the test substance is contacted with the cell that expresses a Kv3.2 protein as
compared to when the test substance is not contacted with the cell that expresses a Kv3.2 protein, the test substance is correlated
with enhancing salty taste, and

(b) when the cation influx is smaller when the test substance is contacted with the cell that expresses a Kv3.2 protein as
compared to when the test substance is not contacted with the cell that expresses a Kv3.2 protein, the test substance is correlated
with inhibiting salty taste,

wherein the Kv3.2 protein has a sequence identity of at least 95% to the sequence of SEQ ID NO: 6, and is able to constitute
a cation channel of which activity changes according to change of extracellular sodium ion concentration.

US Pat. No. 9,308,157

WHITENING COSMETIC

AJINOMOTO CO., INC., Tok...

1. A cosmetic agent, comprising:
(A) at least one cysteine compound represented by formula (I) or a salt thereof:
wherein
X and Y are each independently OR1 or NHR2 wherein R1 and R2 are each independently a hydrogen atom or a C1-22 alkyl group;

Z is a hydrogen atom or a C 1-22 alkyl group; and

W is a C1-22 alkyl group, a C1-22 alkoxy group or a C 1-22 alkylamino group; and

(B) at least one whitening agent,
wherein:
(A) said at least one cysteine compound or a salt thereof is one or more members selected from the group consisting of N-acetyl-2-methylthiazolidine-2,4-dicarboxylic
acid, a salt of N-acetyl-2-methylthiazolidine-2,4-dicarboxylic acid, N-acetyl-2-methylthiazolidine-2,4-dicarboxylic acid 2-ethyl
ester, and a salt of N-acetyl-2-methylthiazolidine-2,4-dicarboxylic acid 2-ethyl ester; and

(B) said at least one whitening agent is one or more members selected from the group consisting of arbutin, cetyl ascorbyl
ether, kojic acid, 4-methoxy salicylic acid, chamomilla recutita extract, ellagic acid, linoleic acid, glutathione, tranexamic
acid, 4-n-butylresorcinol, and 4-hexylresorcinol.

US Pat. No. 9,295,624

AMIDE DERIVATIVE AND WHITENING AGENT

AJINOMOTO CO., INC., Tok...

1. A method of whitening skin, comprising applying a whitening agent to the skin, wherein said whitening agent comprises a
compound represented by formula (V):

wherein:
R1, R2, R3, R4 and R5 are each independently a hydrogen atom, an alkyl group having a carbon number of 1 to 3, a hydroxyl group or an alkoxy group
having a carbon number of 1 to 3, or R1 and R2, or R2 and R3 in combination optionally form a methylenedioxy group, and at least one of R1, R2, R3, R4 and R5 is a hydroxyl group;

R6 is a hydroxyl group and R7 is a hydrogen atom;

Z is a hydrogen atom;
or a salt thereof,
provided that the following compound and salts thereof are excluded:
a compound wherein, when R1, R2 and R5 are hydrogen atoms, then:

R3 and R4 are hydroxyl groups, or

R3 is a hydroxyl group and R4 is a methoxy group,

or R3 and R4 are hydrogen atoms, or

R3 is a methoxy group and R4 is a hydrogen atom.

US Pat. No. 9,309,486

CLEANING AGENT COMPOSITION

AJINOMOTO CO., INC., Chu...

1. A cleansing composition, comprising:
at least one compound represented by formula (1):

where R1 is a saturated or unsaturated, linear or branched-chain hydrocarbon group having 11 to 25 carbon atoms, and R2 and R3 are each independently a hydrogen atom or an alkyl group having 1 to 6 carbon atoms, or a salt thereof and wherein the cleansing
composition is formulated as a cosmetic agent.

US Pat. No. 9,376,695

L-AMINO ACID PRODUCING BACTERIUM

AJINOMOTO CO., INC., Tok...

1. A recombinant L-amino acid producing Escherichia coli bacterium having a DNA comprising:
A) a gene selected from the group consisting of tyrA, pheA, pgi, ilvE, ilvA, tdcB, sdaA, sdaB, argA, argG, proB, thrB, and
combinations thereof, and

B) a DNA fragment able to be transcribed and encoding the peptide of SEQ ID NO: 2, or a variant thereof consisting of a deletion,
insertion, substitution, or addition of 1 amino acid as compared to SEQ ID NO: 2,

wherein said DNA fragment of B) is attached to the 3? end of said gene of A) and consequently enhances production of an L-amino
acid.

US Pat. No. 9,265,273

FEED ADDITIVE COMPOSITION FOR RUMINANTS AND METHOD OF PRODUCING THE SAME

AJINOMOTO CO., INC., Tok...

1. A feed additive composition, comprising:
at least one protective agent;
lecithin in an amount of 0.05 to 6% by weight;
at least one basic amino acid in an amount of at least 40% by weight and less than 65% by weight; and
water in an amount of 2 to 6% by weight;
wherein:
the at least one protective agent comprises at least one member selected from the group consisting of a hydrogenated vegetable
oil having a melting point of greater than 50° C. and less than 90° C. and a hydrogenated animal oil having a melting point
of greater than 50° C. and less than 90° C.; and

the feed additive composition exhibits a water activity of from 0.25 to 0.6.

US Pat. No. 9,427,388

CLEANSING AGENT COMPOSITION COMPRISING SULFONATE-TYPE SURFACTANT AND/OR SULFATE-TYPE ANIONIC SURFACTANT AND HETEROCYCLIC COMPOUND

AJINOMOTO CO., INC., Tok...

1. A cleansing agent composition, comprising:
(A) at least one surfactant selected from the group consisting of a sulfonate surfactant, a sulfate anionic surfactant, and
a mixture thereof; and

(B) at least one heterocyclic compound represented by formula (1):

wherein R1 and R2 are each independently a hydrogen atom, a methyl group, a hydroxymethyl group, or a hydroxyethyl group.

US Pat. No. 9,284,584

METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE HAVING ENHANCED EXPRESSION OF THE FLAGELLA FORMATION AND MOTILITY CASCADE GENES

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid comprising culturing an Escherichia coli bacterium in a medium, and collecting the L-amino acid from the medium,
wherein the bacterium is able to produce an L-amino acid and has been modified so that expression of either the flhD gene
or flhC gene, or both, is/are enhanced by a method selected from the group consisting of: a) increasing the copy number of
the gene(s), b) modifying an expression control region of the gene(s), and c) combinations thereof;

wherein the flhD gene encodes a protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence shown in SEQ ID NO: 2;
(B) a protein comprising the amino acid sequence shown in SEQ ID NO: 2, but wherein one to five amino acid residues are substituted,
deleted, inserted, added or inverted, and said protein has DNA-binding transcriptional dual regulator activity according to
the amino acid sequence of SEQ ID NO: 2, and

(C) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
2, and said protein has DNA-binding transcriptional dual regulator activity according to the amino acid sequence of SEQ ID
NO: 2, and

(D) combinations thereof;
wherein the flhC gene encodes a protein selected from the group consisting of:
(i) a protein comprising the amino acid sequence shown in SEQ ID NO: 4,
(ii) a protein comprising the amino acid sequence shown in SEQ ID NO: 4, but wherein one to five amino acid residues are substituted,
deleted, inserted, added or inverted, and said protein has DNA-binding transcriptional dual regulator activity according to
the amino acid sequence of SEQ ID NO: 4,

(iii) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID
NO: 4, and said protein has DNA-binding transcriptional dual regulator activity according to the amino acid sequence of SEQ
ID NO: 4, and

(iv) combinations thereof.

US Pat. No. 9,181,574

L-AMINO ACID OXIDASE, METHOD FOR MEASURING L-LYSINE, KIT AND ENZYME SENSOR

AJINOMOTO CO., INC., Tok...

1. An isolated lysine oxidase selected from the group consisting of:
(1) a lysine oxidase consisting of the amino acid sequence of SEQ ID NO: 2, but wherein the cysteine at position 254 has been
replaced with a replacement amino acid selected from the group consisting of methionine, phenylalanine, tyrosine, tryptophan,
alanine, glycine, valine, isoleucine, leucine, lysine, arginine, histidine, aspartic acid, glutamic acid, serine, threonine,
asparagine, glutamine, and proline; and

(2) a lysine oxidase comprising an amino acid sequence having at least 90% sequence identity with the amino acid sequence
of SEQ ID NO: 2, wherein the amino acid at position 254 is replaced with a replacement amino acid selected from the group
consisting of methionine, phenylalanine, tyrosine, tryptophan, alanine, glycine, valine, isoleucine, leucine, lysine, arginine,
histidine, aspartic acid, glutamic acid, serine, threonine, asparagine, glutamine, and proline;

wherein said lysine oxidase has oxidase activity with higher substrate specificity for L-lysine than a lysine oxidase consisting
of the amino acid sequence of SEQ ID NO: 2.

US Pat. No. 9,175,319

METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE HAVING ATTENUATED EXPRESSION OF THE YJJK GENE

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid comprising:
(i) cultivating a bacterium belonging to the genus Escherichia in a culture medium to produce said L-amino acid in the culture medium, and

(ii) collecting said L-amino acid from the culture medium,
wherein said Escherichia bacterium has been modified to attenuate expression of a yjjK gene as compared with a corresponding non-modified Escherichia bacterium,

wherein said modification to attenuate expression of said yjjK gene is by a method selected from the group consisting of inactivation
of said yjjK gene on the chromosome of said Escherichia bacterium, modification of a region controlling expression of said yjjK gene on the chromosome of said Escherichia bacterium, and combinations thereof,

wherein said yjjK gene comprises the nucleotide sequence of SEQ ID NO: 1 or a variant nucleotide sequence of SEQ ID NO: 1,
wherein the variant nucleotide sequence is not less than 95% homologous to the nucleotide sequence of SEQ ID NO: 1.

US Pat. No. 10,076,029

METHOD FOR PRODUCING PRINTED WIRING BOARD

Ajinomoto Co., Inc., Tok...

1. A method for producing a printed wiring board, comprising the following steps (A) to (C) in this order:(A) laminating, onto an internal layer substrate, an adhesive sheet which comprises a support formed of polyethylene terephthalate and a resin composition layer in contact with the support, so that the resin composition layer is in contact with the internal layer substrate;
(B) thermally curing said resin composition layer to form an insulating layer; and
(C) removing said support,
wherein
when said support is heated under the following heating condition, said support satisfies the following conditions (TD1) and (TD2) in a TD direction thereof,
heating condition: a temperature is increased from 20° C. to 100° C. at a rate of 8° C./minute, maintained at 100° C. for 30 minutes, then increased to 180° C. at a rate of 8° C./minute, and maintained at 180° C. for 30 minutes,
condition (TD1): a maximum expansion rate EATD (%) is 0.9% or less, and
Condition (TD2): a difference between the maximum expansion rate EATD (%) and an expansion rate at the end of heating EBTD (%), EATD?EBTD, is 0.5% or less.
US Pat. No. 9,334,302

METHOD FOR REMOVING FMOC GROUP

AJINOMOTO CO., INC., Chu...

1. A method of removing an Fmoc group, comprising:
mixing an amino group-containing compound protected by an Fmoc group, a base, and a compound represented by formula (I):
HS-L-COOH  (I)
where L is a C1-8 alkylene group optionally having at least one substituent, to obtain a reaction mixture comprising an amino group-containing
compound and a compound represented by formula (II):

Fm—S-L-COOH  (II)
where Fm is a 9-fluorenylmethyl group; and
washing the reaction mixture with a basic aqueous solution such that the compound represented by the formula (II) is removed;
wherein the amino group-containing compound protected by an Fmoc group, and the amino group-containing compound, do not have
a free carboxy group.

US Pat. No. 9,279,137

MUTANT ACETOLACTATE SYNTHASE AND A METHOD FOR PRODUCING BRANCHED-CHAIN L-AMINO ACIDS

AJINOMOTO CO., INC., Tok...

1. A method for producing a branched-chain L-amino acid comprising cultivating a recombinant microorganism from the Enterobacteriaceae
family in a culture medium, and collecting the branched-chain L-amino acids from the culture medium,
wherein said recombinant microorganism has been transformed with a vector comprising an isolated DNA coding for a mutant small
subunit of bacterial acetolactate synthase (AHAS I) or the isolated DNA has been introduced into the chromosome of said recombinant
microorganism,

wherein the amino acid sequence of said mutant small subunit comprises the amino acid sequence shown in SEQ ID NO: 2, and
also comprises a mutation selected from the group consisting of:

A) replacing the L-amino acid at position 17 in the amino acid sequence of SEQ ID NO: 2 with a lysine residue,
B) replacing the L-amino acid at position 30 in the amino acid sequence of SEQ ID NO: 2 with another L-amino acid,
C) replacing the sequence from the L-amino acid at position 44 to the C-terminus in the amino acid sequence of SEQ ID NO:
2 with 1 to 10 L-amino acids, and

D) combinations thereof; and
wherein said bacterial acetolactate synthase comprising said mutant small subunit is expressed, and is desensitized to feedback
inhibition by valine.

US Pat. No. 9,376,701

METHOD FOR SECRETORY PRODUCTION OF PROTEIN

AJINOMOTO CO., INC., Tok...

1. A method for producing a heterologous protein comprising:
A) culturing a coryneform bacterium having a genetic construct for secretory expression of a heterologous protein;
B) allowing the bacterium to produce and secrete the heterologous protein, and
C) collecting the heterologous protein,wherein the genetic construct comprises:
i) a promoter sequence that functions in the coryneform bacterium,
ii) a first nucleic acid sequence coding for a signal peptide that functions in the coryneform bacterium, wherein said first
nucleic acid sequence is ligated downstream from the promoter sequence, and

iii) a second nucleic acid sequence coding for a fusion protein having:
a) an amino acid sequence comprising Gln-Glu-Thr, and
b) the heterologous protein,wherein said second nucleic acid sequence is ligated downstream from the first nucleic acid sequence coding for the signal
peptide, andwherein the amino acid sequence comprising Gln-Glu-Thr does not consist of an amino acid sequence consisting of the amino
acid residues at positions 1 to 14 or positions 1 to 38 of SEQ ID NO: 96.

US Pat. No. 9,241,503

FEED ADDITIVE COMPOSITION FOR RUMINANTS AND METHOD OF PRODUCING THE SAME

Ajinomoto Co., Inc., Tok...

1. A method of producing a feed additive composition, comprising:
(i) preparing a molten mixture comprising at least one protective agent, lecithin, and at least one basic amino acid; and
(ii) obtaining a solidified mixture by immersing the molten mixture in water;
wherein:
the at least one protective agent is selected from the group consisting of a hardened vegetable oil having a melting point
higher than 50° C. and lower than 90° C. and a hardened animal oil having a melting point higher than 50° C. and lower than
90° C.; and

the feed additive composition is a dispersion type composition.
US Pat. No. 9,394,346

METHOD FOR PRODUCING AN AMINO ACID USING A BACTERIUM OVEREXPRESSING AN RHTC GENE

AJINOMOTO CO., INC., Tok...

1. A method of producing an L-amino acid selected from the group consisting of L-homoserine and L-threonine comprising the
steps of:
A) cultivating in a culture medium an Escherichia coli which has been modified to increase expression of a DNA, wherein expression of said DNA is increased by increasing the copy
number of said DNA or replacing a promoter sequence of said DNA with a promoter sequence which functions in said Escherichia coli, wherein said DNA comprises the nucleotide sequence of nucleotides 187 to 804 of SEQ ID NO: 3;

B) removing solids including cells from the medium; and
C) purifying said L-amino acid from the medium obtained in step B).
US Pat. No. 9,347,047

MODIFIED LEUCINE DEHYDROGENASE

AJINOMOTO CO., INC., Tok...

1. A modified leucine dehydrogenase enzyme comprising at least one amino acid mutation as compared to a non-modified leucine
dehydrogenase enzyme, wherein said modified leucine dehydrogenase is improved in one or more properties selected from the
group consisting of:
(a) substrate specificities for L-leucine, L-isoleucine, and L-valine;
(b) activity for any branched-chain amino acid;
(c) thermal stability, and
(d) combinations thereof,wherein said modified leucine dehydrogenase is:
(A) a protein comprising the amino acid sequence of SEQ ID NO: 2; but having a substitution of isoleucine in the TGI motif
with an amino acid selected from the group consisting of methionine, arginine, histidine, phenylalanine, leucine, lysine,
cysteine, tyrosine, alanine, glycine, serine, asparagine, and tryptophan,

(B) a protein comprising the amino acid sequence of SEQ ID NO: 2; but having a substitution of isoleucine in the GVI motif
with an amino acid selected from the group consisting of phenylalanine, histidine, asparagine, tyrosine, leucine, lysine,
glutamine, arginine, aspartic acid, threonine, glutamic acid, serine, cysteine, alanine, glycine, valine, tryptophan, and
methionine,

(C) a protein comprising the amino acid sequence of SEQ ID NO: 2; but having a substitution of isoleucine in the TGI motif
with an amino acid selected from the group consisting of methionine, arginine, histidine, phenylalanine, leucine, lysine,
cysteine, tyrosine, alanine, glycine, serine, asparagine, and tryptophan; and a substitution of isoleucine in the GVI motif
with an amino acid selected from the group consisting of phenylalanine, histidine, asparagine, tyrosine, leucine, lysine,
glutamine, arginine, aspartic acid, threonine, glutamic acid, serine, cysteine, alanine, glycine, valine, tryptophan, and
methionine, and

(D) a protein as described in (A), (B), or (C) above, but also having one to ten additional mutations of amino acid residues.

US Pat. No. 9,182,407

METHOD OF EVALUATING VISCERAL FAT ACCUMULATION, VISCERAL FAT ACCUMULATION-EVALUATING APPARATUS, VISCERAL FAT ACCUMULATION-EVALUATING METHOD, VISCERAL FAT ACCUMULATION-EVALUATING SYSTEM, VISCERAL FAT ACCUMULATION-EVALUATING PROGRAM

Ajinomoto Co., Inc., Tok...

1. A visceral fat accumulation-evaluating apparatus comprising at least one central processing unit (CPU) and at least one
computer-readable recording medium storing a visceral fat accumulation-evaluating program,
Wherein the CPU executing the visceral fat accumulation-evaluating program is configured to:
calculate a discriminant value that is a value of a multivariate discriminant containing a concentration of at least one amino
acid as an explanatory variable and corresponds to an evaluation result on a visceral fat area state in a subject to be evaluated,
using at least both amino acid concentration data of the subject on a concentration value of the amino acid in blood and the
multivariate discriminant,

wherein the multivariate discriminant contains at least two of Asn, Cit, His, Thr, Val, Leu, Ile, Tyr, Phe, Trp, Glu, Ala,
Pro, Orn, Lys, Gly, Ser, Met, Arg, Cys, and Gln as the explanatory variables; and

wherein the CPU is configured to calculate the discriminant value using at least both the concentration values of at least
two of Asn, Cit, His, Thr, Val, Leu, Ile, Tyr, Phe, Trp, Glu, Ala, Pro, Orn, Lys, Gly, Ser, Met, Arg, Cys, and Gln contained
in the amino acid concentration data of the subject and the multivariate discriminant;

wherein the CPU is further configured to evaluate visceral fat area state in the subject using at least the discriminant value
calculated.

US Pat. No. 9,404,138

METHOD FOR SECRETORY PRODUCTION OF PROTEIN

AJINOMOTO CO., INC., Tok...

1. A coryneform bacterium having an ability to produce a multimeric protein by secretory production, which is modified so
that expression of a gene coding for a metallopeptidase is increased as compared with that in a non-modified strain,
wherein the metallopeptidase is a protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence shown in SEQ ID NO: 4,
(B) a protein comprising the amino acid sequence shown in SEQ ID NO: 4, but which includes substitution, deletion, insertion,
or addition of 1 to 10 amino acid residues, and wherein said protein has a property that if activity thereof is increased
in the coryneform bacterium, the secretory production amount of the multimeric protein is increased compared with that observed
for a non-modified strain; and

wherein the multimeric protein is an antibody-related molecule.
US Pat. No. 9,339,483

AMINO-ACID-CONTAINING MEDICINAL GRANULAR PREPARATION HIGHLY EASY TO TAKE

AJINOMOTO CO., INC., Tok...

1. A granule preparation produced by a process comprising:
preparing granules comprising isoleucine, leucine and valine;
sieving the granules; and
milling the granules,
wherein the granules in the granule preparation have a maximum particle size of not more than 1000 ?m and not less than 355
?m, and a bulk density of not less than 0.59 g/ml and not more than 0.75 g/ml.

US Pat. No. 9,227,949

HETEROARYLCARBOXYLIC ACID ESTER DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. A method for treating hyperglycemia or diabetes, comprising administering an effective amount of a compound represented
by formula (I):
wherein:
R1 and R2 are the same or different and each is independently a C1-4 alkyl group or a C2-4 alkenyl group, or R1 and R2 together with the carbon atom to which they are bonded form a C3-8 cycloalkane ring;

X is —OR3, —NR4R5 or a group represented by formula (II):

wherein:
R3 is a hydrogen atom or a C1-4 alkyl group;

R4, R5 and R6 are the same or different and each is independently a hydrogen atom, a C1-8 alkyl group, a carboxyl C1-8 alkyl group or a C3-8 alkenyl group, or R4 and R5 together with the nitrogen atom to which they are bonded form a C3-9 heterocycle, wherein said C1-8 alkyl group, said C3-8 alkenyl group and said C3-9 heterocycle may be substituted with one or more substituents;

Ra and Rb are the same or different and each is independently a hydrogen atom, a C1-8 alkyl group, a carboxyl C1-8 alkyl group, a carboxyl group, an aryl group, a C3-6 heterocyclic group containing 1 to 4 heteroatoms selected from the group consisting of O, N and S, or a C3-8 cycloalkyl group, or Ra and Rb together with the atom(s) to which they are bonded form a C3-8 cycloalkane ring or a C3-9 heterocycle containing 1-4 heteroatoms selected from the group consisting of O, N and S, wherein said C1-8 alkyl group, said aryl group, said C3-8 cycloalkyl group, said C3-8 cycloalkane ring and said C3-9 heterocycle may be substituted with one or more substituents;

Ring A is an arene, a C3-6 heterocycle containing 1-4 heteroatoms selected from the group consisting of O, N and S, or a C3-8 cycloalkane ring;

Ya is a hydrogen atom, a halogen atom, a carboxyl group, a hydroxyl group, a carbonyl group, a carboxyl C1-3 alkyl group or a sulfo group;

Yb is a hydrogen atom, a halogen atom, a carboxyl group, a hydroxyl group, a carbonyl group, a carboxyl C1-3 alkyl group, a nitro group, a cyano group or a C1-3 alkoxyl group;

p is 0, 1, 2, 3 or 4;
q is 0 or 1; and
R7 is a hydrogen atom, a halogen atom or a nitro group;

with the proviso that when R1 and R2 are both methyl groups, then neither of R4 nor R5 is an ethyl group substituted with two carboxyl groups, and when R1 and R2 are both methyl groups, then the group represented by formula (II) is not a group represented by formula:


or a pharmaceutically acceptable salt thereof,
to a subject in need thereof.
US Pat. No. 9,493,777

METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE HAVING OVEREXPRESSED THE YAJL GENE

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid comprising:
(i) cultivating an L-amino acid-producing bacterium of the family Enterobacteriaceae in a culture medium to produce said L-amino
acid in the bacterium or the culture medium, or both; and,

(ii) collecting said L-amino acid from the bacterium or the culture medium, or both, wherein said bacterium has been modified
to overexpress the yajL gene.

US Pat. No. 9,371,353

OLIGONUCLEOTIDE WITH PROTECTED BASE

AJINOMOTO CO., INC., Tok...

1. A compound comprising a protected base, which is represented by the formula (I):

wherein Base2 is a nucleic acid base protected by a group having a C5-30 branched chain alkyl group and/or a C5-30 branched chain alkenyl group;

P1 is a hydrogen atom, or a temporary protecting group removable under acidic conditions;

X is a hydrogen atom, an optionally protected hydroxyl group, or a halogen atom;
P2 is a protecting group removable under basic conditions; and

Re and Rf are each independently a C1-6 alkyl group, or a 5- or 6-membered saturated cyclic amino group formed together with the adjacent nitrogen atom.

US Pat. No. 9,512,177

METHOD FOR PRODUCING ?-GLUTAMYL-VALYL-GLYCINE CRYSTAL

Ajinomoto Co., Inc., Tok...

1. A method for producing a ?-glutamyl-valyl-glycine crystal, comprising:
reacting valyl-glycine or a salt thereof with a ?-glutamyl group donor in the presence of ?-glutamyl transferase or a ?-glutamyl
transferase crude enzyme extract obtained from a microorganism expressing said transferase, to prepare a mixed solution of
valyl-glycine or the salt thereof and ?-glutamyl-valyl-glycine, in which the mixed solution contains valyl-glycine in an amount
of 20 mass % or more relative to the mass of ?-glutamyl-valyl-glycine;

adjusting the amount of valyl-glycine or the salt thereof in the prepared mixed solution to 0.1 mass % or more and less than
20 mass % relative to the mass of ?-glutamyl-valyl-glycine in the solution to prepare a ?-glutamyl-valyl-glycine solution;
and

subjecting the ?-glutamyl-valyl-glycine solution to a crystallization procedure, comprising adding a poor solvent to the solution
and then cooling the solution, to produce the ?-glutamyl-valyl-glycine crystal.

US Pat. No. 9,603,375

PROCESS FOR PRODUCING MATERIAL FOR FOOD OR BEVERAGE

AJINOMOTO CO., INC., Tok...

1. A method of producing a material for food or drink, which comprises:
acclimating a starting oil from a plant or animal by supplying oxygen to said starting oil at a rate of 0.058 to 100 mg/L/min;
and then

heating said oil from a plant or animal while supplying oxygen at a dissolved oxygen supply speed of not less than 0.058 mg/L/min
to said oil from a plant or animal, to obtain a treated oil which contains at least one compound selected from the group consisting
of 5 to 500 weight ppm octanoic acid, 5 to 550 weight ppm 1-octen-3-ol, and 10 to 4200 weight ppm decanoic acid.

US Pat. No. 9,487,806

METHOD FOR PRODUCING L-AMINO ACID

Ajinomoto Co., Inc., Tok...

1. A method for producing an L-amino acid comprising:
culturing an Escherichia coli bacterium having an L-amino acid-producing ability in a medium so that an L-amino acid is produced and accumulates in the
medium or the cells of the Escherichia coli bacterium; and

collecting the L-amino acid from the medium or the cells,
wherein the Escherichia coli bacterium has been genetically modified to have attenuated but not eliminated expression of the chromosomal acyl carrier protein
(acpP) gene as compared to a non-modified Escherichia coli bacterium.

US Pat. No. 9,243,075

N-ACYLAMINO ACID DERIVATIVES OF CELLULOSE OR STARCH BASED POLYSACCHARIDES AND EMULSIFIED PRODUCTS CONTAINING THE SAME

Ajinomoto Co., Inc., Tok...

1. A polysaccharide represented by formula (1):

wherein:
A represents a polysaccharide residue;
R represents an acyl group derived from a saturated or unsaturated fatty acid having 8 to 12 carbon atoms;
—CO—X—NH— represents an amino acid residue selected from the group consisting of aspartic acid and glutamic acid;
n is an integer of 100 to 3,000; and
said polysaccharide has a weight average molecular weight of 1,500,000 to 8,000,000,
wherein A represents one or more selected from the group consisting of a cellulose residue, a starch residue, a hydroxyethylcellulose
residue, a methylcellulose residue, an ethylcellulose residue, and a hydroxypropylcellulose residue, and

wherein said polysaccharide has a degree of dispersion of 1.5 to 50.

US Pat. No. 9,597,275

GELLANT

AJINOMOTO CO., INC., Tok...

1. A gellant for an oily base, comprising at least one peptide compound represented by formula (1):
wherein R1, R2a, R2b, and R2c are the same or different and are each independently an alkyl group, one of a and b is 0 and the other is 2.

US Pat. No. 9,290,487

PHARMACEUTICAL COMPOSITION FOR TREATING DIABETES

AJINOMOTO CO., INC., Chu...

1. A compound of formula (I):

wherein Ar is an aromatic carbocyclic ring or a heterocyclic ring;
Ar2 is represented by any one of the following rings


these rings may have a substituent, and
the substituent is selected from the group consisting of acetamido, aminocarbonyl, benzyl, benzyloxy, a halogen, hydroxyl-lower
alkyl, lower alkyl, lower alkoxy-lower alkyl, phenoxy, phenyl, a formyl group, a cyano group, a cyanoalkyl group, a hydroxyiminomethyl
group, a hydroxyamidino group, an amino group, an aminoalkyl group, an alkylaminoalkyl group, a dialkylaminoalkyl group, lower
alkoxy, and trifluoro-methoxy;

R2 and R3 are independently selected from the group consisting of lower alkyl, lower alkoxy, trifluoromethyl, a halogen, hydroxy, a
hydroxyl-lower alkyl group, amino, alkylamino, dialkylamino, cyano, and nitro;

R4 is an amino acid residue bonded to C(O) through a nitrogen atom of the amino acid;

m is 0, 1, 2, 3, or 4;
p is 0, 1, or 2; and
s is 0, 1, or 2,
or a pharmaceutically acceptable salt thereof.

US Pat. No. 9,206,230

BENZYLIC COMPOUND

AJINOMOTO CO., INC., Tok...

1. A method of producing a peptide by a liquid phase synthesis process, comprising:
(1) removing a protecting group of a N-terminal of a C-protected amino acid or C-protected peptide, wherein a benzylic compound
represented by formula (I):

wherein
Y is a hydroxyl group or an —NHR group;
R is a hydrogen atom, an alkyl group or an aralkyl group;
Ra is an organic group having an aliphatic hydrocarbon group, which has a total carbon number of not less than 14;

each Rb is independently an alkoxy group having a carbon number of 1 to 6, a halogen atom, or an alkyl group having a carbon number
of 1 to 6, which is optionally substituted by one or more halogen atoms; and

n is an integer of 0-4,
is condensed with a C-terminal of a N-protected amino acid or N-protected peptide;
(2) condensing the N-terminal of the amino acid or peptide from (1) with a N-protected amino acid or N-protected peptide to
produce a peptide, and

(3) precipitating the peptide from (2).

US Pat. No. 9,658,234

METHOD FOR ANALYSIS OF COMPOUNDS WITH AMINO GROUP AND ANALYTICAL REAGENT THEREFOR

AJINOMOTO CO., INC., Tok...

6. A method for analyzing a compound with an amino group in a sample, containing at least a compound with an amino group by
means of mass spectrometry, said method comprising
labeling said compound with an amino group in said sample by reacting said compound with an amino group with a carbamate compound
according to claim 1, to obtain a mixture comprising a labeled compound; and

subjecting said labeled compound to mass spectrometry.
US Pat. No. 9,522,104

GELLING AGENT

AJINOMOTO CO., INC., Tok...

1. A gelling agent, comprising:
at least one compound selected from the group consisting of N-2-ethylhexanoyl glutamic acid dibutylamide and N-lauroyl glutamic
acid dibutylamide,

wherein the compound has a DL form ratio (D form/L form (weight/weight)) of 5/95 to 20/80.

US Pat. No. 9,810,674

METHOD FOR EVALUATING FOOD PREFERENCE OF PETS

Ajinomoto Co., Inc., Chu...

1. A method of evaluating a food preference of a dog, the food preference being expressed in human sensory words, the method
comprising:
selecting a plurality of palatants, where the plurality of palatants includes a roast flavor type hydrolyzed soybean protein,
a beer yeast extract, a meat-like flavor material, a hydrolyzed soybean protein, a torula yeast extract, a beef extract, a
dried bonito extract, and a cheese paste;

determining a plurality of sensory attributes indicating taste, aroma, and flavor, where the sensory attributes indicating
taste consist of sour taste, bitter taste, salty taste, sweet taste, umami taste, mildness, taste intensity, and taste persistence,
the sensory attributes indicating aroma consist of sour aroma, fermentation aroma, roast aroma, sweet aroma, fishy aroma,
and sulfurous aroma, and the sensory attributes indicating flavor consist of soy sauce-roasted flavor, ocean and dried fish
flavor, animal-like flavor, and sour flavor;

performing a sensory analysis of the palatants on a human such that the human tastes each of the palatants and scores an intensity
of each of the sensory attributes as a sensory analysis score for each of the palatants;

feeding each of the palatants to a plurality of dogs such that a preference test is performed on the dogs;
generating a three-dimensional map including plots indicating the dogs and plots indicating the palatants based on results
of the preference test, such that each dogs' preference on each of the palatants is indicated by a distance between coordinates
of the plots indicating the palatants and coordinates of the plots indicating the dogs, respectively;

selecting contributory attributes from the sensory attributes showing a statistically significant correlation with at least
one of the palatants;

drawing arrows indicating a respective one of the contributory attributes in the three-dimensional map, based on the sensory
analysis score;

determining a coordinate of a linking optimum (LO) in the three-dimensional map such that a total of distances between the
coordinate of the LO and each of the coordinates of the plots indicating the dogs is minimized; and

calculating an optimal score for each of the contributory attributes by regressing the coordinate of the LO to coordinates
of plots indicating each of the palatants,

wherein the human tastes only the palatants in the method.
US Pat. No. 9,506,094

METHOD FOR PRODUCING L-AMINO ACID USING MICROORGANISM HAVING INCREASED PHOSPHATE TRANSPORTER ACTIVITY

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid comprising:
(A) culturing a coryneform bacterium having an L-amino acid producing ability in a medium; and
(B) collecting the L-amino acid from the medium,
wherein the bacterium has been modified to increase the expression of a gene encoding a phosphate transporter, wherein the
increased expression of the gene is obtained by increasing the copy number of the gene and/or modifying an expression control
sequence of the gene, wherein said phosphate transporter is selected from the group consisting of:

(a) a protein which comprises the amino acid sequence of SEQ ID NO: 6 or SEQ ID NO: 26,
(b) a protein which comprises all of SEQ ID NO: 6 or 26 except for the substitution, deletion, insertion or addition of 1
to 10 amino acid residues, wherein said protein has phosphate transporter activity,

(c) a protein which comprises all of SEQ ID NO: 6 or 26 except for the replacement of the amino acid residue at a position
corresponding to position 246 of SEQ ID NO: 6 with a serine residue, and

(d) a protein which comprises all of SEQ ID NO: 6 or 26 except for (i) the replacement of the amino acid residue at a position
corresponding to position 246 of SEQ ID NO: 6 with a serine residue, and (ii) the substitution, deletion, insertion or addition
of 1 to 10 amino acid residues, wherein said protein has phosphate transporter activity.

US Pat. No. 9,465,031

METHOD OF EVALUATING PROSTATIC DISEASE

Ajinomoto Co., Inc., Tok...

1. A method of evaluating prostatic disease, comprising:
obtaining concentration values of amino acids in blood of a subject to be evaluated;
evaluating, by a central processing unit (CPU) executing a prostatic disease-evaluating program stored on a computer-readable
recording medium, a state of prostatic disease including at least one of prostatic cancer and prostatic hypertrophy in the
subject, using at least concentration values of at least Trp and Ala contained in amino acid concentration data on the concentration
values of the amino acids in blood of the subject,

wherein the concentration value criterion evaluating step further includes:
a discriminant value calculating step of calculating, by the CPU, a discriminant value that is a value of a multivariate discriminant,
using at least both (i) the concentration values of at least Trp and Ala contained in the amino acid concentration data of
the subject and (ii) the multivariate discriminant for evaluating the state of prostatic disease containing at least Trp and
Ala as explanatory variables.

US Pat. No. 9,453,206

MODIFIED LEUCINE DEHYDROGENASE

AJINOMOTO CO., INC., Tok...

1. A modified leucine dehydrogenase enzyme comprising at least one amino acid mutation as compared to a non-modified leucine
dehydrogenase enzyme, wherein said modified leucine dehydrogenase is improved in one or more properties selected from the
group consisting of:
(a) substrate specificities for L-leucine, L-isoleucine and L-valine;
(b) activity for any branched-chain amino acid;
(c) thermal stability, and
(d) combinations thereof,
wherein said modified leucine dehydrogenase is selected from the group consisting of:
(A) a protein comprising the amino acid sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 6, and SEQ
ID NO: 8; but having a substitution of isoleucine in the TGI motif with an amino acid selected from the group consisting of
methionine, arginine, histidine, phenylalanine, leucine, lysine, cysteine, tyrosine, alanine, glycine, serine, asparagine,
and tryptophan,

(B) a protein comprising the amino acid sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 6, and SEQ
ID NO: 8; but having a substitution of isoleucine in the GVI motif with an amino acid selected from the group consisting of
phenylalanine, histidine, asparagine, tyrosine, leucine, lysine, glutamine, arginine, aspartic acid, threonine, glutamic acid,
serine, cysteine, alanine, glycine, valine, tryptophan, and methionine,

(C) a protein comprising the amino acid sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 6, and SEQ
ID NO: 8; but having a substitution of isoleucine in the TGI motif with an amino acid selected from the group consisting of
methionine, arginine, histidine, phenylalanine, leucine, lysine, cysteine, tyrosine, alanine, glycine, serine, asparagine,
and tryptophan; and a substitution of isoleucine in the GVI motif with an amino acid selected from the group consisting of
phenylalanine, histidine, asparagine, tyrosine, leucine, lysine, glutamine, arginine, aspartic acid, threonine, glutamic acid,
serine, cysteine, alanine, glycine, valine, tryptophan, and methionine, and

(D) a protein as described in (A), (B), or (C) above, but also having one to ten additional mutations of amino acid residues.

US Pat. No. 9,587,259

ESCHERICHIA COLI CAPABLE OF PRODUCING 3-AMINO-4-HYDROXYBENZOIC ACID

AJINOMOTO CO., INC., Tok...

1. A method for producing a 3-amino-4-hydroxybenzoic acid or a salt thereof, comprising a step of culturing an Escherichia coli bacterium having an ability to produce 3-amino-4-hydroxybenzoic acid which has been genetically modified to express DNA which
encodes an enzyme to increase the activity of an enzyme, wherein said enzyme is capable of catalyzing formation of 3-amino-4-hydroxybenzoic
acid from dihydroxyacetone phosphate and aspartate semialdehyde as compared to a non-modified Escherichia coli bacterium, and wherein said enzyme is GriI and GriH; and wherein the Escherichia coli bacterium also has been modified to inactivate an N-hydroxyarylamine O-acetyltransferase (NhoA) as compared to a non-modified
Escherichia coli bacterium by mutating or deleting an nhoA gene on the chromosome of the Escherichia coli bacterium.

US Pat. No. 9,353,147

METHOD FOR PRODUCING PEPTIDE

AJINOMOTO CO., INC., Chu...

1. A method of producing a peptide, comprising:
(1) removing an N-terminal Fmoc group from (a) N-Fmoc C-protected amino acid or (b) an N-Fmoc C-protected peptide, with a
non-nucleophilic organic base in a halogenated solvent or ether solvent to obtain a mixture comprising (a?) a C-protected
amino acid or (b?) a C-protected peptide;

(2) neutralizing said mixture with an acid, to obtain a neutralized mixture;
(3) adding (c) an N-Fmoc amino acid or (d) an N-Fmoc peptide, a condensing agent and a condensation accelerator to said neutralized
mixture; and

(4) condensing the N-terminal of either of said (a?) C-protected amino acid or said (b?) C-protected peptide with either of
said (c) N-Fmoc amino acid or said (d) N-Fmoc peptide to obtain (e) an N-Fmoc C-protected peptide,

wherein, when said (1) removing an N-terminal Fmoc group comprises removing an N-terminal Fmoc group from (b) an N-Fmoc C-protected
peptide, said (b) N-Fmoc C-protected peptide is different from said (e) N-Fmoc C-protected peptide,

wherein a C-terminal carboxy group either of said (a) N-Fmoc C-protected amino acid or said (b) N-Fmoc C-protected peptide
is protected by an anchor group, and

wherein said (1) removing, said (2) neutralizing, said (3) adding, and said (4) condensing are performed without any intervening
isolation of said (a?) C-protected amino acid or said (b?) C-protected peptide, and

wherein said anchor group is a group represented by formula (II):

wherein:
R1 is a hydrogen atom or, when Rb is a group represented by the following formula (a), optionally forms a single bond together with R3 to form a fluorene ring together with ring A and ring B;

each R2 is independently an organic group having an aliphatic hydrocarbon group;

p is an integer of 1 to 4;
ring A optionally further has, in addition to OR2, a substituent selected from the group consisting of a halogen atom, a C1-6 alkyl group optionally substituted by a halogen atom, and a C1-6 alkoxy group optionally substituted by a halogen atom;

Ra is a hydrogen atom, or a phenyl group optionally substituted by a halogen atom; and

Rb is a hydrogen atom, or a group represented by formula (a):


wherein:
* is the point of binding to the remainder of the molecule;
r is an integer of 0 to 4;
each R4 is independently an organic group having an aliphatic hydrocarbon group;

R3 is a hydrogen atom, or optionally forms a single bond together with R1 to form a fluorene ring together with ring A and ring B; and

ring B optionally further has, in addition to OR4, a substituent selected from the group consisting of a halogen atom, a C1-6 alkyl group optionally substituted by a halogen atom, and a C1-6 alkoxy group optionally substituted by a halogen atom; and

L represents a connection to the carbonyl group of said C terminus and is —O— or —N(R)—, wherein R is a hydrogen atom, an
alkyl group, or an aralkyl group.

US Pat. No. 10,257,928

RESIN SHEET

Ajinomoto Co., Inc., Tok...

1. A resin sheet, comprising:a support; and
a resin composition layer being in contact on the support,whereinan extracted water conductivity A of a cured product of the resin composition layer when extracted at 120° C. for 20 hours is 50 ?S/cm or less and an extracted water conductivity B of the cured product of the resin composition layer when extracted at 160° C. for 20 hours is 200 ?S/cm or less.
US Pat. No. 9,638,706

STANDARD SOLUTION FOR USE IN ANALYSIS OF AMINO ACID IN PLASMA

WAKO PURE CHEMICAL INDUST...

1. An external standard solution comprising:
(1) water or buffer,
(2) at least one amino acid selected from the following Components A, at a concentration of 0.0007 M to 0.49 M per amino acid;
and

(3) at least one of (i), (ii), or (iii):
(i) at least one amino acid selected from the following Components B, wherein each amino acid selected from Components B has
a concentration of 0.2 to 0.9 times that of the lowest-concentration amino acid among amino acids selected from Components
A,

(ii) at least one amino acid selected from the following Components C, wherein each amino acid selected from Components C
has a concentration of 0.1 to 0.4 times that of the lowest-concentration amino acid among amino acids selected from Components
A, and when the external standard solution includes an amino acid selected from Components B, has a concentration of less
than 1 times that of the lowest-concentration amino acid among amino acids selected from Components B, or

(iii) at least one amino acid selected from the following Components D, wherein each amino acid selected from Components D
has a concentration of 0.05 to 0.2 times that of the lowest-concentration amino acid among amino acids selected from Components
A, and when the external standard solution includes an amino acid selected from Components B, has a concentration of less
than 1 times that of the lowest-concentration amino acid among amino acids selected from Components B, and when the external
standard solution includes Components C, has a concentration of less than 1 times that of the lowest-concentration amino acid
among amino acids selected from Components C;

wherein the Components A are valine, glycine, alanine, and glutamine;
wherein the Components B are serine, proline, threonine, taurine, leucine, isoleucine, lysine, histidine, phenylalanine, and
tyrosine;

wherein the Components C are asparagine, ornithine, arginine, and tryptophan; and
wherein the Components D are glutamic acid, methionine, citrulline, and cystine.

US Pat. No. 9,499,579

BRANCHED CHAIN-CONTAINING AROMATIC COMPOUND

AJINOMOTO CO., INC., Tok...

1. A method of producing a peptide comprising:
(1) removing an N-terminal protecting group from (i) an N-protected C-protected amino acid or (ii) a first N-protected C-protected
peptide, wherein said N-protected C-protected amino acid and said first N-protected C-protected peptide have a C-terminus
protected with an anchor which is a protecting group derived from a branched chain-containing aromatic compound, to obtain
a C-protected amino acid or C-protected peptide;

(2) condensing an N-protected amino acid or N-protected peptide with the N-terminus of said C-protected amino acid or C-protected
peptide to obtain a second N-protected C-protected peptide; and

(3) removing the N-terminal protecting group and the C-terminal anchor from said second N-protected C-protected peptide, to
obtain said peptide,

wherein said branched chain-containing aromatic compound is represented by formula (I):

wherein
each Q is independently a single bond, or —O—, —S—, —C(?O)O—, —C(?O)NH— or —NH—;
each Ra is independently an organic group having at least one aliphatic hydrocarbon group having one or more branched chains, wherein
a total number of branched chains in each Ra is not less than 3 and a total carbon number in each Ra is not less than 14 and not more than 300;

k is an integer of 1 to 4;
R1 is a hydrogen atom or, when Z is a group represented by formula (a), it optionally forms a single bond together with R2 to form a fluorene ring together with ring B;

ring A optionally further has, in addition to R1, (QRa)k, and C(X)(Y)Z, one or more substituents selected from the group consisting of a halogen atom, a C1-6 alkyl group optionally substituted by one or more halogen atoms, and a C1-6 alkoxy group optionally substituted by one or more halogen atoms;

X is a hydrogen atom or a phenyl group;
Y is a hydroxyl group, an —NHR group (wherein R is a hydrogen atom, an alkyl group, or an aralkyl group), or a halogen atom;
and

Z is a hydrogen atom or a group represented by formula (a):

wherein
* indicates the bonding position;
m is an integer of 0 to 4;
each Q is as defined above;
each Rb is independently an organic group having at least one aliphatic hydrocarbon group having one or more branched chains, wherein
a total number of the branched chain in each Rb is not less than 3 and a total carbon number in each Rb is not less than 14 and not more than 300;

R2 is a hydrogen atom, or optionally forms a single bond together with R1 to form a fluorene ring together with ring A; and

ring B optionally further has, in addition to (QRb)m and R2, one or more substituents selected from the group consisting of a halogen atom, a C1-6 alkyl group optionally substituted by one or more halogen atoms, and a C1-6 alkoxy group optionally substituted by one or more halogen atoms;

wherein said organic group having at least one aliphatic hydrocarbon group having one or more branched chains, a total number
of the branched chain of not less than 3 and a total carbon number of not less than 14 and not more than 300 for each Ra and Rb is a group having 3 or more of the same or different divalent groups represented by formula (b):


wherein
* indicates the bonding position with the adjacent atom;
R3 and R4 are each independently a hydrogen atom or a C1-4 alkyl group;

X1 is a single bond, a C1-4 alkylene group, or an oxygen atom,

provided that R3 and R4 are not hydrogen atoms at the same time.

US Pat. No. 9,459,255

METHOD OF EVALUATING BREAST CANCER, BREAST CANCER-EVALUATING APPARATUS, BREAST CANCER-EVALUATING METHOD, BREAST CANCER-EVALUATING SYSTEM, BREAST CANCER-EVALUATING PROGRAM AND RECORDING MEDIUM

Ajinomoto Co., Inc., Tok...

1. A method of evaluating breast cancer, comprising:
a concentration value criterion evaluating step of evaluating, by a central processing unit (CPU) executing a breast cancer-evaluating
program stored on a computer-readable recording medium, a breast cancer state in a subject to be evaluated, using at least
concentration values of total Trp and at least one of Ser, Gln, Val, Cys, Orn, Arg, Ile, ABA, Ala, Asn, Cit, Glu, Gly, His,
Leu, Lys, Met, Phe, Pro, Tau, Thr, and Tyr contained in amino acid concentration data on the concentration values of the amino
acids in blood in the subject;

wherein the concentration value criterion evaluating step further includes:
a discriminant value calculating step of calculating, by the CPU, a discriminant value that is a value of a multivariate discriminant,
using at least both the concentration values of total Trp and at least one of Ser, Gln, Val, Cys, Orn, Arg, Ile, ABA, Ala,
Asn, Cit, Glu, Gly, His, Leu, Lys, Met, Phe, Pro, Tau, Thr, and Tyr contained in the amino acid concentration data of the
subject and the multivariate discriminant for evaluating the breast cancer state containing total Trp and at least one of
Ser, Gln, Val, Cys, Orn, Arg, Ile, ABA, Ala, Asn, Cit, Glu, Gly, His, Leu, Lys, Met, Phe, Pro, Tau, Thr, and Tyr as explanatory
variables.

US Pat. No. 9,580,696

METHOD FOR PRODUCING ?-GLUTAMYLVALYLGLYCINE OR A SALT THEREOF

Ajinomoto Co., Inc., Chu...

1. A method for producing ?-Glu-Val-Gly comprising reacting Val-Gly with a ?-glutamyl group donor in the presence of a ?-glutamyltransferase
such that ?-Glu-Val-Gly is generated, wherein the ?-glutamyltransferase comprises a large subunit and a small subunit, wherein
the large subunit has an amino acid sequence having no less than 90% identity to the amino acid sequence of the positions
26 to 390 of the amino acid sequence of SEQ ID NO: 2, wherein the glutamic acid at the position corresponding to position
38 of SEQ ID NO: 2 is modified by a substitution, wherein the small subunit has an amino acid sequence having no less than
90% sequence identity to the amino acid sequence of the positions 391 to 580 of the amino acid sequence of SEQ ID NO: 2, and
wherein the tyrosine at the position corresponding to position 444 of SEQ ID NO: 2 is modified by a substitution.
US Pat. No. 9,533,940

METHOD FOR PRESERVING 1,5-PENTANEDIAMINE OR SALT THEREOF, METHOD FOR PREVENTING DISCOLORATION OF 1,5-PENTANEDIAMINE OR SALT THEREOF, AND 1,5-PENTANEDIAMINE OR SALT THEREOF IN CONTAINER

AJINOMOTO CO., INC., Tok...

1. A method for preserving purified 1,5-pentanediamine or a salt thereof which has a water content, the method comprising:
adjusting said water content in the purified 1,5-pentanediamine or a salt thereof to more than 1% by weight and not more than
50% by weight.

US Pat. No. 9,708,639

METHOD FOR QUANTIFYING AMINO ACIDS WITH PYROPHOSPHATE

TOYAMA PREFECTURE, Toyam...

1. A method for quantifying an amino acid, which comprises:
(A) reacting an aminoacyl-tRNA synthetase (AARS) corresponding to the amino acid with the amino acid and adenosine triphosphate
(ATP) in the presence of an (aminoacyl-adenosine monophosphate (AMP))-AARS complex decomposition reagent to generate pyrophosphate,
wherein the AARS is regenerated in free form via decomposition of a formed (aminoacyl-AMP)-AARS complex,

(B) quantifying the pyrophosphate generated in (A) by a method comprising reacting pyrophosphate dikinase (PPDK) with the
generated pyrophosphate in the presence of AMP and phosphoenolpyruvate (PEP) to generate ATP, phosphoric acid, and pyruvate,
and

(C) quantifying the generated pyruvate;
wherein the amount of the amino acid is determined based on the amount of pyruvate,
and wherein said method is performed in the absence of tRNA.
US Pat. No. 9,599,618

METHOD, APPARATUS, SYSTEM, PROGRAM, AND COMPUTER-READABLE RECORDING MEDIUM FOR EVALUATING COLORECTAL CANCER

Ajinomoto Co., Inc., Tok...

1. A method of evaluating colorectal cancer, comprising:
a concentration value criterion evaluating step of evaluating a colorectal cancer state in a subject to be evaluated, by a
central processing unit (CPU) executing a colorectal cancer-evaluating program stored on a computer-readable recording medium,
using at least a concentration value of at least Val contained in amino acid concentration data on the concentration value
of the amino acid in blood of the subject;

wherein the concentration value criterion evaluating step further includes:
a discriminant value calculating step of calculating, by the CPU, a discriminant value that is a value of a multivariate discriminant,
using at least both the concentration value of at least Val contained in the amino acid concentration data of the subject
and the multivariate discriminant for evaluating the colorectal cancer state containing at least Val as an explanatory variable.

US Pat. No. 10,000,760

YEAST WITH HIGH CONTENT OF ABU, ?-GLU-ABU, AND/OR ?-GLU-ABU-GLY

AJINOMOTO CO., INC., Tok...

1. Yeast, comprising:60 ?mol/g-DCW or more of an ?-aminobutyric acid (Abu)-related compound consisting of at least one compound selected from the group consisting of Abu, ?-Glu-Abu, and ?-Glu-Abu-Gly,
wherein the yeast is Saccharomyces cerevisiae and has been modified such that intracellular activity of acetolactate synthase encoded by ILV2 gene in the yeast is decreased to 10% or less of intracellular activity of a corresponding enzyme in the wild type of the yeast, and that the yeast has increased activity of at least one of ?-ketobutyric acid synthase and aminotransferase as compared to the wild type of the yeast,
wherein the ?-ketobutyric acid synthase is selected from the group consisting of:
(a) a protein comprising the amino acid sequence of SEQ ID NO: 18;
(b) a protein comprising a variant of the amino acid sequence of SEQ ID NO: 18, which has substitution, deletion, insertion, or addition of 1 to 10 amino acid residues in the amino acid sequence of SEQ ID NO: 18, and having ?-ketobutyric acid synthase activity; and
(c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO: 18, and having ?-ketobutyric acid synthase activity, and
wherein the aminotransferase is selected from the group consisting of:
(d) a protein comprising the amino acid sequence of SEQ ID NO: 22;
(e) a protein comprising a variant of the amino acid sequence of SEQ ID NO: 22, which has substitution, deletion, insertion, or addition of 1 to 10 amino acid residues in the amino acid sequence of SEQ ID NO: 22, and having aminotransferase activity; and
(f) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO: 22, and having aminotransferase activity.
US Pat. No. 9,890,359

CULTURE MEDIUM FOR PROLIFERATING STEM CELL, WHICH CONTAINS SULFATED COMPOUND

AJINOMOTO CO., INC., Tok...

1. A method for culturing a stem cell, comprising culturing the stem cell in a medium comprising:
a fibroblast growth factor (FGF), and
a sulfated compound or a pharmaceutically acceptable salt thereof,
wherein said sulfated compound or a pharmaceutically acceptable salt thereof is present in said medium at a concentration
that promotes the growth of said stem cell in the presence of FGF, and

wherein said sulfated compound or a pharmaceutically acceptable salt thereof is a sulfated substance of a saccharide polymer
crosslinked by a diisocyanate compound or a pharmaceutically acceptable salt thereof.

US Pat. No. 9,574,216

AUTO-INDICIBLE EXPRESSION SYSTEM, AND THE USE THEREOF FOR PRODUCING USEFUL METABOLITES USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE

AJINOMOTO CO., INC., Tok...

1. A gene expression system comprising:
A) LysR-type protein-regulated transcriptional machinery comprising a promoter and an operator, the expression of the transcriptional
machinery is positively regulated by the LysR-type regulatory protein and a coinducer, and

B) a gene(s) of interest to which said transcriptional machinery is operably linked, wherein the gene(s) of interest encode(s)
a protein(s) involved in biosynthesis of said coinducer, a substrate, or a precursor of said coinducer, whereby auto-inducible
positive feedback regulation of said expression system is mediated by said coinducer, wherein said promoter is PilvC promoter, said LysR-type regulatory protein is IlvY protein, and said coinducer is 2-acetolactatic acid or a salt thereof,
or 2-aceto-2-hydroxybutyric acid or a salt thereof, and wherein said gene(s) of interest encode(s) acetohydroxy-acid synthetase.

US Pat. No. 9,670,121

DIPHENYLMETHANE COMPOUND

AJINOMOTO CO., INC., Tok...

1. A diphenylmethane compound represented by formula (I):

wherein
Y is a hydroxyl group;
k and l are each independently an integer of 0-5 and k+l is not 0;
Ra in the number of k and Rb in the number of l are each independently an organic group having an aliphatic hydrocarbon group, wherein, in the organic
group(s) in the number of (k+l), each having an aliphatic hydrocarbon group, the total carbon number of the aliphatic hydrocarbon
groups is not less than 16;

ring A optionally further has substituent(s) besides Ra; and

ring B optionally further has substituent(s) besides Rb,

wherein the organic group is independently bonded directly to ring A or ring B via —O—, —S—, —COO—, —OCONH— or —CONH—.
US Pat. No. 9,560,862

PROCESS FOR PRODUCING MATERIAL FOR FOOD OR BEVERAGE

AJINOMOTO CO., INC., Tok...

1. A method of producing a material for food or drink, which comprises:
acclimating a starting oil from a plant or animal by supplying oxygen to said starting oil at a rate of 0.058 to 100 mg/L/min;
and then

heating said oil from a plant or animal while supplying oxygen at a dissolved oxygen supply speed of not less than 0.058 mg/L/min
to said oil from a plant or animal, to obtain a treated oil which contains at least one compound selected from the group consisting
of 5 to 500 weight ppm octanoic acid, 5 to 550 weight ppm 1-octen-3-ol, and 10 to 4200 weight ppm decanoic acid.

US Pat. No. 9,844,226

USE OF PEPTIDES FOR IMPARTING KOKUMI

AJINOMOTO CO., INC., Tok...

1. A kokumi-imparting agent comprising, in combination,
(a) ?-Glu-Abu; and
(b) peptides selected from the group consisting of ?-Glu-X-Gly wherein X represents an amino acid or an amino acid derivative,
?-Glu-Val-Y wherein Y represents an amino acid or an amino acid derivative, ?-Glu-Ala, ?-Glu-Gly, ?-Glu-Cys, ?-Glu-Met, ?-Glu-Thr,
?-Glu-Val, ?-Glu-Orn, Asp-Gly, Cys-Gly, Cys-Met, Glu-Cys, Gly-Cys, Leu-Asp, D-Cys, ?-Glu-Met (O), ?-Glu-?-Glu-Val, ?-Glu-Val-NH2, ?-Glu-Val-ol, ?-Glu-Ser, ?-Glu-Tau, ?-Glu-Cys (S-Me) (O), ?-Glu-Leu, ?-Glu-Ile, ?-Glu-t-Leu, ?-Glu-Cys (S-Me), and combinations
thereof;

wherein ?-Glu-Abu is present in said agent in an amount of not less than 1,000 ppm by mass.

US Pat. No. 9,782,336

MOISTURIZER AND COSMETIC CONTAINING SAME

AJINOMOTO CO., INC., Tok...

1. A composition, comprising:
(A) at least one acylproline represented by formula (1) or a salt thereof
wherein R1—CO— is an acyl group derived from a saturated or unsaturated fatty acid having 3 to 23 carbon atoms; and
(B) a zinc salt of pyrrolidone carboxylate.
US Pat. No. 9,664,681

LUNG CANCER EVALUATING APPARATUS, METHOD, SYSTEM, AND PROGRAM AND RECORDING MEDIUM THEREFOR

Ajinomoto Co., Inc., Tok...

1. A method of evaluating lung cancer, comprising:
a concentration value criterion evaluating step of evaluating, by a central processing unit (CPU) executing a lung cancer-evaluating
program stored on a computer-readable recording medium, a lung cancer state in a subject to be evaluated, based on concentration
values of at least Lys and His contained in amino acid concentration data on the concentration values of the at least two
amino acids in blood of the subject,

wherein the concentration value criterion evaluating step further includes a discriminant value calculating step of calculating,
by the CPU, a discriminant value that is a value of a multivariate discriminant, based on both (i) the concentration values
of at least Lys and His and (ii) the multivariate discriminant for evaluating the lung cancer state containing at least Lys
and His explanatory variables.

US Pat. No. 9,629,386

NUTRITION COMPOSITION

AJINOMOTO CO., INC., Tok...

1. A method for preventing or improving at least one symptom selected from the group consisting of malnutrition, inflammation,
arteriosclerosis, abnormal lipid metabolism and oxidative stress, associated with renal disease, comprising:
administering, to a subject in need thereof, 50 kcal to 2000 kcal of a nutrition composition for an adult per day,
wherein the nutrition composition comprises at least one free amino acid selected from the group consisting of valine, leucine,
isoleucine, and histidine, a lipid comprising at least one ?-3 fatty acid and at least one ?-6 fatty acid in a weight ratio
of ?-6 fatty acid to ?-3 fatty acid of 0.5 to 5.5, a carbohydrate, and a soybean protein or a hydrolysate thereof,

in the nutrition composition, the total amount of protein and peptide is not more than 3.5 g per 100 kcal of the nutrition
composition, the soybean protein or a hydrolysate thereof comprises 20 wt % to 100 wt % of any proteins or peptides present,
a content of the protein and peptide is 6 calorie % to 10 calorie %, a content of the lipid is 30 calorie % to 32 calorie
%, and a content of the carbohydrate is 60 calorie % to 62 calorie %, and

the subject in need thereof is a patient having at least one symptom selected from the group consisting of malnutrition, inflammation,
arteriosclerosis, abnormal lipid metabolism and oxidative stress, associated with renal disease.

US Pat. No. 9,617,202

METHOD FOR PRODUCING 1,5-PENTANEDIAMINE

AJINOMOTO CO., INC., Tok...

1. A method for producing 1,5-pentanediamine in a free form from a salt of 1,5-pentanediamine comprising:
A) applying a solution of a salt of 1,5-pentanediamine to an ion exchange resin column in a downflow mode thereby allowing
adsorption of 1,5-pentanediamine in free form onto the column; and

B) applying an eluent solution to the column in an upflow mode, thereby eluting a solution of the 1,5-pentanediamine in free
form from the column, wherein the eluent solution is sodium hydroxide or potassium hydroxide,

wherein the salt of 1,5-pentanediamine is a hydrochloride or a sulfate.
US Pat. No. 9,926,348

METHOD FOR PRODUCING FIBROIN-LIKE PROTEIN

Ajinomoto Co., Inc., Tok...

1. A method for producing a fibroin-like protein, the method comprising:(A) culturing an Escherichia coli bacterium having a gene encoding the fibroin-like protein in a medium,
(B) inducing expression of the gene encoding the fibroin-like protein while reducing accumulation of an organic acid, and
(C) collecting the fibroin-like protein;
wherein said reducing accumulation of the organic acid is initiated by limiting a carbon source in the medium before inducing the expression, and
wherein the organic acid is selected from the group consisting of acetic acid, citric acid, succinic acid, formic acid, and combinations thereof.
US Pat. No. 9,873,898

METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE HAVING ATTENUATED EXPRESSION OF A PHOSPHATE TRANSPORTER-ENCODING GENE

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid selected from the group consisting of L-His, L-Arg, or combinations thereof, said
method comprising:
(i) cultivating an L-amino acid-producing Escherichia coli bacterium in a culture medium to produce and accumulate an L-amino acid in the culture medium or cells of the bacterium, or
both; and

(ii) collecting the L-amino acid from the culture medium or cells of the bacterium, or both,
wherein said bacterium has been modified to attenuate expression of a phosphate transporter-encoding gene and
wherein said phosphate transporter-encoding gene is selected from the group consisting of:
(A) a DNA comprising the nucleotide sequence of SEQ ID NO: 1;
(B) a DNA comprising the nucleotide sequence of SEQ ID NO: 3;
(C) a DNA comprising a variant nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3 due to the degeneracy of the genetic code;
(D) a DNA having an identity of the nucleotide sequence of not less than 75% with respect to the entire nucleotide sequence
of SEQ ID NO: 1 or SEQ ID NO: 3, and wherein said nucleotide sequence encodes a protein having inorganic phosphate-transporting
activity;

(E) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 2;
(F) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 4; and
(G) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4, but wherein said sequence
includes substitutions, deletions, insertions, or additions of 1 to 30 amino acid residues, and wherein said protein has inorganic
phosphate-transporting activity.

US Pat. No. 9,840,725

METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE HAVING A DISRUPTED PUTRESCINE DEGRADATION PATHWAY

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid comprising:
(i) cultivating an L-amino acid-producing bacterium of the family Enterobacteriaceae in a culture medium; and
(ii) collecting said L-amino acid in the culture medium, wherein said bacterium has been modified to disrupt a putrescine
degradation pathway,

wherein said bacterium belongs to the genus Escherichia, and

wherein said putrescine degradation pathway is disrupted by a method selected from the group consisting of:
(a) attenuation of expression of a gene selected from the group consisting of patA, patD, gabT, gabD, puuA, puuB, puuC, puuD,
puuE, and combinations thereof, by mutating said gene, and

(b) attenuation of expression of at least one gene from the puuADRCBE gene cluster by mutating said gene, with the proviso
that the puuR gene cannot be the only gene that is attenuated; and

wherein said L-amino acid is L-arginine or L-omithine.
US Pat. No. 9,822,385

METHOD FOR PRODUCING AN L-GLUTAMIC ACID AND L-ASPARTIC ACID USING A RECOMBINANT MICROORGANISM HAVING ENHANCED EXPRESSION OF A YBJL PROTEIN

AJINOMOTO CO., INC., Tok...

1. A method for producing an acidic substance having a carboxyl group comprising
culturing in a medium a microorganism, having an ability to produce the acidic substance having a carboxyl group, to produce
and accumulate the acidic substance having a carboxyl group in the medium,

collecting the acidic substance having a carboxyl group from the medium,
wherein the acidic substance is L-glutamic acid and/or L-aspartic acid,
wherein the microorganism has been modified to enhance expression of an ybjL gene, and
wherein the ybjL gene encodes a protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence of SEQ ID NO: 2; and
(B) a protein comprising a variant of the amino acid sequence of SEQ ID NO: 2, wherein one to 5 amino acid residues are substituted,
deleted, inserted or added, and wherein, upon expression, said variant improves the ability of the microorganism to produce
the acidic substance having a carboxyl group.

US Pat. No. 9,786,450

MEMBRANE SWITCH AND OBJECT EMPLOYING SAME

AJINOMOTO CO., INC., Tok...

1. A membrane switch, comprising a first conductive part formed on a first substrate and a second conductive part formed on
a second substrate, wherein the first substrate and the second substrate are layered via a spacer such that said first and
second conductive parts face each other with a space therebetween,
wherein
an organic material showing piezoelectricity is filled in said space, or
an organic material showing piezoelectricity is disposed in said space such that an air gap is present between said organic
material and one of said first and second conductive parts, and

wherein said spacer is in direct contact with each of the first substrate and the second substrate.
US Pat. No. 9,708,637

METHOD FOR PRODUCING LOWER ALKYL ESTER

AJINOMOTO CO., INC., Tok...

1. A method for producing a lower alkyl ester of ?-L-aspartyl-L-phenylalanine, comprising:
A) cultivating a bacterium of Escherichia coli in a culture medium which is able to produce and accumulate L-phenylalanine in the medium, and

B) synthesizing the lower alkyl ester of ?-L-aspartyl-L-phenylalanine from aspartic acid or a derivative thereof and the L-phenylalanine
obtained in step A);

wherein the bacterium comprises a DNA comprising:
i) a gene selected from the group consisting of tyrA, pheA, pgi, ilvE, ilvA, tdcB, sdaA, sdaB, argA, argG, proB, thrB, and
combinations thereof, and

ii) a DNA fragment able to be transcribed and encoding the peptide of SEQ ID NO: 2, or a variant thereof consisting of a deletion,
insertion, substitution, or addition of 1 amino acid as compared to SEQ ID NO: 2; and

wherein said DNA fragment of ii) is attached to the 3? end of said gene of i) and consequently enhances production of an L-amino
acid.

US Pat. No. 9,644,009

L-AMINO ACID-PRODUCING BACTERIUM AND A METHOD FOR PRODUCING AN L-AMINO ACID

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid comprising:
A) culturing in a medium a bacterium of the family Enterobacteriaceae which has an ability to produce an L-amino acid and
which has been modified to increase the expression of a gene selected from the group consisting of evgA, gadE, ydeO, and combinations
thereof,

B) producing and accumulating the L-amino acid in the medium, and
C) collecting the L-amino acid from the medium,
wherein expression of said gene(s) is/are increased by a method selected from the group consisting of:
a) increasing the copy number of said gene(s),
b) modifying an expression regulatory sequence of said gene(s),
c) modifying the bacterium so that the expression of an evgS gene that encodes a sensor kinase is increased by a method selected
from the group consisting of:

i) increasing the copy number of the evgS gene,
ii) modifying the expression regulatory sequence of the evgS gene, and
iii) combinations thereof; and
d) combinations thereof, and
wherein said L-amino acid is selected from the group consisting of a basic amino acid, a hydroxymonoaminocarboxylic acid,
and an aromatic amino acid,

wherein the basic amino acid is selected from the group consisting of L-lysine, L-ornithine, L-arginine, L-histidine, and
L-citrulline;

wherein the hydroxymonoaminocarboxylic acid is selected from the group consisting of L-threonine and L-serine; and
wherein the aromatic amino acid is selected from the group consisting of L-tryptophan, L-phenylalanine, and L-tyrosine.

US Pat. No. 10,053,491

METHOD FOR PRODUCING PEPTIDE HYDRAZIDE, PEPTIDE AMIDE, AND PEPTIDE THIOESTER

AJINOMOTO CO., INC., Tok...

1. A method for producing a peptide hydrazide compound of Formula (1):
wherein R1 represents an amino acid residue or a derivative thereof, or a peptide residue or a derivative thereof,
the method comprising:
(A) reacting a compound of Formula (2a) with an alcohol compound in the presence of a transition metal compound comprising a nickel compound, such that an ester compound of Formula (3) is obtained,

wherein R1 is as defined above,
R2 represents hydrogen or alkyl,
R4 represents an arginine side chain, a lysine side chain, or a histidine side chain,
R5 represents a histidine side chain,
R6 represents a leucine side chain, an isoleucine side chain, a tyrosine side chain, a phenylalanine side chain, an arginine side chain, or a tryptophan side chain, and
R7 represents hydroxyl, amino, hydrazino, or an organic group,

wherein R1 is as defined above, and
R8 represents alkyl; and
(B) reacting the ester compound of Formula (3) with
a hydrazine compound.
US Pat. No. 9,890,373

MODIFIED ISOPRENE SYNTHASE

Ajinomoto Co., Inc., Tok...

1. A modified isoprene synthase, having an amino acid sequence at least 90 identical to the amino acid sequence of SEQ ID
NO: 4,
wherein the modified isoprene synthase has at least one mutation of an amino acid residue corresponding to at least one amino
acid residue in SEQ ID NO: 4 selected from the group consisting of: C286, E321, C370, A390, E471, C480, I518, and C521, and

the modified isoprene synthase has an isoprene synthetic activity.

US Pat. No. 9,750,676

BASIC AMINO ACID DERIVATIVE THAT DEMONSTRATES A GELLING ABILITY IN A WATER SYSTEM

AJINOMOTO CO., INC., Tok...

1. A compound represented by formula (1 A):

wherein
R1—CO— is an acyl group derived from an optionally substituted saturated or unsaturated fatty acid having 6 to 18 carbon atoms;

R2 and R3 are each independently an optionally substituted saturated or unsaturated, straight chain or branched chain hydrocarbon group
having 1 to 6 carbon atoms, or

R2 and R3 form, together with the nitrogen atom to which they are bonded, an optionally substituted heterocycle;

R4, R5, and R6 are each independently a hydrogen atom or an optionally substituted saturated or unsaturated, straight chain or branched chain
hydrocarbon group having 1 to 6 carbon atoms;

m is an integer of 1 to 20;
n is an integer of 1 to 4;
X is a sulfonic acid group or a carboxylic acid group; and
Y is an optionally substituted saturated or unsaturated, straight chain or branched chain divalent hydrocarbon group having
1 to 8 carbon atoms,

or a salt thereof.
US Pat. No. 9,617,299

METHOD FOR PRODUCING PROTEIN BY PRECIPITATION

AJINOMOTO CO., INC., Cho...

1. A method of purifying a fusion protein, comprising:
(1) adjusting the pH of
an aqueous phase, comprising a fusion protein which is a fusion of a protein having a self-assembly capability and a target
protein, and which aqueous phase has a first pH, to a second pH, to obtain a remaining aqueous phase and a solid fraction
comprising an amount of said fusion protein;

(2) separating said solid fraction from said remaining aqueous phase, to obtain a separated solid fraction; and
(3) dissolving said separated solid fraction in a solution having a pH of 12 or lower but higher than said second pH by at
least 0.1 pH units,

wherein said protein having a self-assembly capability is a cell surface protein that is a CspB mature protein or a portion
thereof; and

wherein (i) said target protein has 10 to 300 amino acid residues, or (ii) the ratio of the number of amino acid residues
in the target protein to the number of amino acid residues in the CspB mature protein or portion thereof is in a range of
0.3 to 14.

US Pat. No. 9,952,235

STANDARD SOLUTION FOR USE IN ANALYSIS OF AMINO ACIDS IN PLASMA

WAKO PURE CHEMICAL INDUST...

1. An internal standard solution comprising: proline, glycine, valine, methionine, tryptophan, tyrosine, and taurine, wherein one or more atoms of each amino acid is labeled with a stable isotope, and wherein proline has a lower concentration in the internal standard solution than glycine, valine, methionine, tryptophan, tyrosine, and taurine.
US Pat. No. 9,896,704

METHOD FOR PRODUCING L-ISOLEUCINE USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE HAVING OVEREXPRESSED THE CYCA GENE

AJINOMOTO CO., INC., Tok...

1. A method for producing L-isoleucine comprising:
(i) cultivating an Escherichia coli bacterium that produces L-isoleucine in a culture medium or in cells of the bacterium, or both; and

(ii) collecting the L-isoleucine from the culture medium or the cells, or both,
wherein the bacterium has been modified to overexpress a cycA gene by increasing the copy number of the cycA gene and/or by
modifying an expression regulatory region of the cycA gene, so that the expression of said gene is enhanced as compared with
a non-modified bacterium.

US Pat. No. 9,872,499

VIRUS-INACTIVATING COMPOSITION CONTAINING LOW-MOLECULAR WEIGHT COMPOUND AND ARGININE

AJINOMOTO CO., INC., Tok...

1. A method of inactivating lipid-envelope viruses on a virus contaminated surface in need thereof, wherein the surface is
an outer surface of living tissue of humans or animals or is a surface of a substrate, and wherein said viruses are proliferating
on the surface, the method comprising bringing a composition with pH of 3.8 to 5.5 into contact with the virus contaminated
surface,
wherein the composition comprises:
(A) 0.115M to 0.287M arginine, and
(B) a component (B) selected from the group consisting of:
i) 0.01 to 0.5 mM of (?)epicatechin gallate or (?)epigallocatechin gallate;
ii) 0.01 to 0.1 mM caffeic acid phenethyl ester;
iii) 0.1 to 10 mM dehydroascorbic acid; and
iv) 0.005 to 0.02 mass % of cocoyl arginine ethyl ester; and
wherein said viruses are selected from the group consisting of influenza and herpes.
US Pat. No. 9,695,460

METHOD OF ANALYZING L-TRYPTOPHAN IN BIOLOGICAL SAMPLES, AND KIT USED THEREIN

Public University Corpora...

1. A method for analyzing L-tryptophan in a specimen that contains L-tryptophan and L-phenylalanine, comprising the steps
of:
(A) mixing the specimen, an L-tryptophan oxidase, and water, producing a reaction solution,
(B) allowing the reaction solution to stand for a prescribed period in the presence of oxygen, producing at least one type
of reaction product; and either

(C) confirming the presence of the at least one type of reaction product due to the action of the L-tryptophan oxidase present
in the reaction solution after standing for the prescribed period, or

(D) measuring the quantity of the at least one type of the reaction product;
wherein the L-tryptophan oxidase has the amino acid sequence of SEQ ID NO: 1.
US Pat. No. 10,136,669

METHOD FOR DECREASING VISCERAL FAT OR INCREASING ENERGY CONSUMPTION

AJINOMOTO CO., INC., Tok...

1. A method for decreasing visceral fat or increasing energy consumption, comprising administering to a subject in need thereof an effective amount of a composition comprising:i) n-3 fatty acid, and
ii) an ingredient selected from the group consisting of free lysine, dipeptides containing lysine and lysine salts, and combinations thereof;
wherein the ingredient is present in the composition in an amount of 0.1 g-10.0 g per 100 kcal of the composition, and
wherein the n-3 fatty acid is present in the composition in an amount of 0.17 g-5.00 g per 100 kcal of the composition.

US Pat. No. 10,100,008

IMMUNOSTIMULATING AGENT

AJINOMOTO CO., INC., Tok...

1. An immunostimulating method, comprising administering to a subject in need thereof an effective amount of at least one kind of compound represented by formula (I):whereinR1 and R2 are the same or different and each is a C1-6 alkyl group;
R3 and R4 are the same or different and each is a C12-37 alkyl group;
X1 is —O—, —NR5— wherein R5 is a hydrogen atom or a C1-6 alkyl group, or —S—; and
X2 is —O—, —NR6— wherein R6 is a hydrogen atom or a C1-6 alkyl group, or —S—.
US Pat. No. 10,047,385

METHOD FOR MANUFACTURING USEFUL SUBSTANCE

AJINOMOTO CO., INC., Tok...

1. A method for producing an objective substance, the method comprising:culturing a microorganism having an objective substance-producing ability in a medium to produce and accumulate the objective substance in the medium or in cells of the microorganism; and
collecting the objective substance from the medium or the cells,
wherein the microorganism has been modified so that the activity of a dicarboxylic acid exporter protein is reduced;
wherein the gene encoding the dicarboxylic acid exporter protein is selected from the group consisting of yjjP gene, yjjB gene, yeeA gene, ynfM gene, sucE1 gene, and combinations thereof;
wherein the yjjP gene is a DNA selected from the group consisting of:
(A) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 158 or 160;
(B) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 158 or 160, but including substitution, deletion, insertion, or addition of 1-10 amino acid residues, the protein having an activity to export a dicarboxylic acid;
(C) a DNA comprising the nucleotide sequence of SEQ ID NO: 157 or 159; and
(D) a DNA able to hybridize under stringent conditions with a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 157 or 159, or with a probe that can be prepared from the complementary nucleotide sequence, and encoding a protein having an activity to export a dicarboxylic acid;
wherein the yjjB gene is a DNA selected from the group consisting of:
(A) DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 162 or 164;
(B) DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 162 or 164, but including substitution, deletion, insertion, or addition of 1-10 amino acid residues, the protein having an activity to export a dicarboxylic acid;
(C) DNA comprising the nucleotide sequence of SEQ ID NO: 161 or 163; and
(D) DNA able to hybridize under stringent conditions with a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 161 or 163, or with a probe that can be prepared from the complementary nucleotide sequence, and encoding a protein having an activity to export a dicarboxylic acid;
wherein the yeeA gene is a DNA selected from the group consisting of:
(A) DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 166, 168, or 170;
(B) DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 166, 168, or 170, but including substitution, deletion, insertion, or addition of 1-10 amino acid residues, the protein having an activity to export a dicarboxylic acid;
(C) DNA comprising the nucleotide sequence of SEQ ID NO: 165, 167, or 169; and
(D) DNA able to hybridize under stringent conditions with a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 165, 167, or 169, or with a probe that can be prepared from the complementary nucleotide sequence, and encoding a protein having an activity to export a dicarboxylic acid;
wherein the ynfM gene is a DNA selected from the group consisting of:
(A) DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 172, 174, 176, 178, or 180;
(B) DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 172, 174, 176, 178, or 180, but including substitution, deletion, insertion, or addition of 1-10 amino acid residues, the protein having an activity to export a dicarboxylic acid;
(C) DNA comprising the nucleotide sequence of SEQ ID NO: 171, 173, 175, 177, or 179; and
(D) DNA able to hybridize under stringent conditions with a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 171, 173, 175, 177, or 179, or with a probe that can be prepared from the complementary nucleotide sequence, and encoding a protein having an activity to export a dicarboxylic acid;
wherein the sucE1 gene is a DNA selected from the group consisting of:
(A) DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 278 or 280;
(B) DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 278 or 280, but including substitution, deletion, insertion, or addition of 1-10 amino acid residues, the protein having an activity to export a dicarboxylic acid;
(C) DNA comprising the nucleotide sequence of SEQ ID NO: 277 or 279; and
(D) DNA able to hybridize under stringent conditions with a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 277 or 279, or with a probe that can be prepared from the complementary nucleotide sequence, and encoding a protein having an activity to export a dicarboxylic acid;
wherein said stringent conditions are 0.1×SSC, 0.1% SDS at 68° C.; and
wherein the objective substance is a metabolite derived from acetyl-CoA and/or an L-amino acid.
US Pat. No. 9,976,126

MODIFIED GLYCINE OXIDASE

AJINOMOTO CO., INC., Tok...

1. A glycine oxidase enzyme comprising the amino acid sequence of SEQ ID No: 2, and also comprising a mutation of at least one amino acid residue, wherein said mutation results in the improvement of one or more properties of said glycine oxidase which are associated with measurement of glycine as compared to said glycine oxidase that does not comprise said mutation, wherein the one or more properties are selected from the group consisting of:(a) an activity of the glycine oxidase for glycine;
(b) a thermal stability of the glycine oxidase; and
(c) a substrate specificity of the glycine oxidase for glycine,
wherein the mutation is selected from the group consisting of:
(A) a substitution of the first threonine in a TTS motif with alanine, cysteine, or glycine,
(B) a substitution of serine in the TTS motif with lysine,
(C) a substitution of cysteine in an HCY motif with alanine, aspartic acid, glycine,
histidine, asparagine, tryptophan, tyrosine, or serine,
(D) a substitution of leucine in an LRP motif with isoleucine, valine, cysteine, threonine, or proline,
(E) a substitution of serine with arginine in a SG motif located in at positions 190 to 204 of SEQ ID NO: 2,
(F) a substitution of glycine in a POT motif with tyrosine or glutamine, and
(G) combinations thereof;wherein the glycine oxidase enzyme may have supplemental mutations as long as said supplemental mutations maintain said improved property.
US Pat. No. 9,970,031

METHOD FOR PRODUCING DICARBOXYLIC ACID

AJINOMOTO CO., INC., Tok...

1. A method for producing a dicarboxylic acid, the method comprising:culturing a bacterium having a dicarboxylic acid-producing ability in a medium to produce and accumulate the dicarboxylic acid in the medium; and
collecting the dicarboxylic acid from the medium, wherein the bacterium has been modified to increase the expression of a gene selected from the group consisting of yeeA, ynfM, yjjP, yjjB, and combinations thereof as compared to that in the non-modified bacterium;
wherein the expression of the gene(s) is increased by increasing the copy number of the gene(s) and/or modifying an expression control sequence of the gene(s);
wherein the yeeA gene is a DNA selected from the group consisting of:
(1A) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 2, 4, or 6,
(1B) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 2, 4, or 6, but which includes substitution, deletion, insertion or addition of 1 to 10 amino acid residues, and wherein said protein has a dicarboxylic acid-secreting activity,
(1C) a DNA encoding a protein comprising an amino acid sequence having an identity of 90% or more to the amino acid sequence of SEQ ID NO: 2, 4, or 6, and wherein said protein has a dicarboxylic acid-secreting activity,
(1D) a DNA comprising the nucleotide sequence of SEQ ID NO: 1, 3, or 5, and
(1E) a DNA that hybridizes under stringent conditions of 0.1×SSC, 0.1% SDS at 60° C. with the full-length complement of the polynucleotide of SEQ ID NO: 1, 3, or 5, and encoding a protein having a dicarboxylic acid-secreting activity;
wherein the ynfM gene is a DNA selected from the group consisting of:
(2A) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 8, 10, 12, 14, or 16,
(2B) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 8, 10, 12, 14, or 16, but which includes substitution, deletion, insertion or addition of 1 to 10 amino acid residues, and wherein said protein has a dicarboxylic acid-secreting activity,
(2C) a DNA encoding a protein comprising an amino acid sequence having an identity of 90% or more to the amino acid sequence of SEQ ID NO: 8, 10, 12, 14, or 16, and wherein said protein has a dicarboxylic acid-secreting activity,
(2D) a DNA comprising the nucleotide sequence of SEQ ID NO: 7, 9, 11, 13, or 15, and
(2E) a DNA that hybridizes under stringent conditions of 0.1×SSC, 0.1% SDS at 60° C. with the full-length complement of the polynucleotide of SEQ ID NO: 7, 9, 11, 13, or 15, and encoding a protein having a dicarboxylic acid-secreting activity;
wherein the yjjP gene is a DNA selected from the group consisting of:
(3A) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 18 or 20,
(3B) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 18 or 20, but which includes substitution, deletion, insertion or addition of 1 to 10 amino acid residues, and wherein said protein has a dicarboxylic acid-secreting activity,
(3C) a DNA encoding a protein comprising an amino acid sequence having an identity of 90% or more to the amino acid sequence of SEQ ID NO: 18 or 20, and wherein said protein has a dicarboxylic acid-secreting activity,
(3D) a DNA comprising the nucleotide sequence of SEQ ID NO: 17 or 19, and
(3E) a DNA that hybridizes under stringent conditions of 0.1×SSC, 0.1% SDS at 60° C. with the full-length complement of the polynucleotide of SEQ ID NO: 17 or 19, and encoding a protein having a dicarboxylic acid-secreting activity;
wherein the yjjB gene is a DNA selected from the group consisting of:
(4A) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 22 or 24,
(4B) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 22 or 24, but which includes substitution, deletion, insertion or addition of 1 to 10 amino acid residues, and wherein said protein has a dicarboxylic acid-secreting activity,
(4C) a DNA encoding a protein comprising an amino acid sequence having an identity of 90% or more to the amino acid sequence of SEQ ID NO: 22 or 24, and wherein said protein has a dicarboxylic acid-secreting activity,
(4D) a DNA comprising the nucleotide sequence of SEQ ID NO: 21 or 23, and
(4E) a DNA that hybridizes under stringent conditions of 1×SSC, 0.1% SDS at 60° C. with the full-length complement of the polynucleotide of SEQ ID NO: 21 or 23, and encoding a protein having a dicarboxylic acid-secreting activity;
provided that the bacterium is a Pantoea bacterium, and Enterobacter bacterium, or a coryneform bacterium when the expression of the ynfM is increased.
US Pat. No. 9,708,577

APPARATUS FOR CONTROLLING AMMONIA AND A METHOD FOR CONTROLLING AMMONIA

Ajinomoto Co., Inc., Tok...

1. A method for producing a basic amino acid selected from the group consisting of L-lysine, L-arginine, and L-histidine by
fermentation comprising culturing a microorganism having an ability to produce the basic amino acid in a culture medium contained
in a culture tank to produce and accumulate the basic amino acid-in the culture medium, wherein total ammonia concentration
of the culture medium is continually adjusted to be within a certain concentration range which is sufficient to enhance production
of the basic amino acid during at least a part of the total culture process by using an ammonia-controlling apparatus,
said ammonia-controlling apparatus comprising at least an ammonia feeder that supplies ammonia to the culture tank, an ammonia
sensor, and a control part, wherein:

the ammonia sensor responds to non-ionized ammonia in the culture medium contained in the culture tank,
the control part comprises a processing unit and is connected to the ammonia feeder and the ammonia sensor, and
the control part is configured to
(i) create a calibration curve representing a relation between non-ionized ammonia concentration of the culture medium and
a signal from the ammonia sensor,

(ii) calculate non-ionized ammonia concentration of the culture medium from the signal from the ammonia sensor by using the
calibration curve, and

(iii) transmit an electric signal to the ammonia feeder when the calculated non-ionized ammonia concentration is lower than
a predetermined concentration thereby directing the ammonia feeder to supply ammonia to the culture tank.

US Pat. No. 9,711,446

RESIN COMPOSITION

Ajinomoto Co., Inc., Tok...

1. A resin composition, comprising:
(A) an epoxy resin;
(B) a curing agent; and
(C) an inorganic filler,
Wherein a content of said inorganic filler is 55% by mass or higher with respect to 100% by mass of a non-volatile component within the resin
composition'

An average particle diameter of said inorganic filler is 0.05 to 0.35 ?m,
A product of a specific surface area (m2/g) of said inorganic filler and a true Density (g/cm3) of said inorganic filler is 1 to 77, and

a moisture permeation of a cured product obtained by thermally curing said resin Composition is 0.05 to 2.8 g·mm/m2·34 h, wherein the product of the specific Surface area of said component and the true density of said component is 26 to 77.

US Pat. No. 9,975,928

HEPAROSAN-PRODUCING BACTERIUM AND HEPAROSAN MANUFACTURING METHOD

AJINOMOTO CO., INC., Tok...

1. A method for producing heparin, the method comprising:A) culturing an Escherichia coli bacterium having a heparosan-producing ability in a medium to produce and accumulate heparosan in the medium;
B) treating the heparosan using chemical methods, enzymatic methods, or both chemical and enzymatic methods to produce heparin; and
C) collecting the heparin;
wherein the bacterium has been modified so that expression is increased of an Escherichia coli rpoE gene;
wherein the bacterium has been further modified so that expression of an Escherchia coli heparosan biosynthesis enzyme gene is increased.
US Pat. No. 9,971,866

METHOD OF EVALUATING FATTY LIVER RELATED DISEASE, FATTY LIVER RELATED DISEASE-EVALUATING APPARATUS, FATTY LIVER RELATED DISEASE-EVALUATING METHOD, FATTY LIVER RELATED DISEASE-EVALUATING PROGRAM PRODUCT, FATTY LIVER RELATED DISEASE-EVALUATING SYSTEM, INFOR

Ajinomoto Co., Inc., Tok...

1. A method of evaluating fatty liver related disease, comprising:evaluating, by a central processing unit (“CPU”) executing a fatty liver related disease-evaluating program stored on a computer-readable recording medium, a state of a fatty liver related disease including at least one of fatty liver, NAFLD (non-alcoholic fatty liver disease), and NASH (non-alcoholic steatohepatitis) in a subject to be evaluated, based on amino acid concentration data on a concentration value of an amino acid in blood collected from the subject; wherein the fatty liver related disease-evaluating program is configured to evaluate the state of the NAFLD in the subject based on the concentration value of at least one of Gln, Pro, Gly, Ala, Cit, Leu, Val, Phe, Met, His, Trp, Orn, Ser, Thr, and Asn contained in the amino acid concentration data.
US Pat. No. 9,932,614

METHOD FOR PRODUCING L-AMINO ACID OF GLUTAMATE FAMILY

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid, the method comprising:A) culturing a coryneform bacterium having an L-amino acid-producing ability in a medium; and
B) collecting the L-amino acid from the medium, wherein the bacterium has been modified so that the activity of an ?-ketoglutaric acid (?-KG) uptake carrier is increased as compared with a non-modified bacterium, wherein the ?-KG uptake carrier is a protein encoded by kgtP gene, and
wherein the L-amino acid is an L-amino acid of glutamate family.

US Pat. No. 9,790,497

MORPHOLINO OLIGONUCLEOTIDE MANUFACTURING METHOD

AJINOMOTO CO., INC., Tok...

1. A morpholino nucleotide represented by formula (I):
wherein
m is any integer of not less than 0;
each of m+1 Base is independently an optionally protected nucleic acid base;
P1 is a hydrogen atom, or a temporary protecting group removable under acidic conditions;

each of m X is independently a C1-6 alkoxy group, a di-C1-6 alkylamino group, or a piperazino group wherein a nitrogen atom at the 4-position is protected by a protecting group and further
optionally substituted;

each of m W is independently an oxygen atom or a sulfur atom;
S1 is a single bond, or a group represented by *O—S2**, wherein * indicates the bonding position to L, ** indicates the bonding position to a 5?-hydroxy group, and S2 is a spacer having a main chain containing 1 to 20 atoms;

L is a single bond, or a group represented by the formula (a1):
wherein
* indicates the bonding position to Y;
** indicates the bonding position to S1;

L1 is an optionally substituted divalent C1-22 hydrocarbon group; and

L2 is C(?O) or a group represented by ***N(R3)—R1—N(R2)C(?O)**, wherein ** indicates the bonding position to L1, *** indicates the bonding position to Y, R1 is an optionally substituted C1-22 alkylene group, R2 and R3 are each independently a hydrogen atom or an optionally substituted C1-22 alkyl group, or R2 and R3 are optionally joined to form an optionally substituted C1-22 alkylene bond;

Y is a single bond, an oxygen atom or NR, wherein R is a hydrogen atom, an alkyl group or an aralkyl group; and
Z is a group represented by formula (a2):
wherein
* indicates the bonding position to Y;
R4 is a hydrogen atom, or when Rb is a group represented by the following formula (a3), R4 is optionally a single bond or —O— in combination with R6 to form a fluorenyl group or a xanthenyl group together with ring B;

each of k Q is independently a single bond, or —O—, —S—, —OC(?O)—, —NHC(?O)— or —NH—;
each of k R5 is independently an organic group containing an alkyl group having not less than 10 and not more than 300 carbon atoms and/or
an alkenyl group containing not less than 10 and not more than 300 carbon atoms;

k is an integer of 1 to 4;
ring A optionally further has, in addition to k of QR5, a substituent selected from the group consisting of a halogen atom, a C1-6 alkyl group optionally substituted by one or more halogen atoms, and a C1-6 alkoxy group optionally substituted by one or more halogen atoms;

Ra is a hydrogen atom;

Rb is a hydrogen atom, or a group represented by formula (a3):

wherein
* indicates a bonding position;
j is an integer of 0 to 4;
each of j Q is as defined above;
each of j R7 is independently an organic group having an alkyl group containing not less than 10 and not more than 300 carbon atoms and/or
an alkenyl group containing not less than 10 and not more than 300 carbon atoms;

R6 is a hydrogen atom, or optionally a single bond or —O— in combination with R4 to form a fluorenyl group or a xanthenyl group together with ring A; and

ring B optionally further has, in addition to j of QR7, a substituent selected from the group consisting of a halogen atom, a C1-6 alkyl group optionally substituted by one or more halogen atoms, and a C1-6 alkoxy group optionally substituted by one or more halogen atoms, or

Ra and Rb are joined to form an oxygen atom.

US Pat. No. 10,113,161

MUTANT GLUTAMATE-CYSTEINE LIGASE AND METHOD FOR MANUFACTURING GAMMA GLUTAMYL-VALYL-GLYCINE

AJINOMOTO CO., INC., Tok...

1. A method for producing ?-Glu-Val and/or a salt thereof, the method comprising:contacting a mutant glutamate-cysteine ligase with Glu and Val to produce ?-Glu-Val and/or a salt thereof,
wherein the mutant glutamate-cysteine ligase comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2 wherein the amino acid residue corresponding to at least one residue selected from the group consisting of L135, Q144, Y241, N243, and Y300 of SEQ ID NO: 2 is mutated to a different amino acid in the mutant glutamate-cysteine ligase, and
wherein the mutant has ?-glutamylvaline synthetase activity.
US Pat. No. 9,907,766

SWEETNESS RECEPTOR ANTAGONIST

AJINOMOTO CO., INC., Tok...

1. 1-(4-Cyanophenyl)-3-(cyclohexylmethyl)thiourea or a salt thereof.
US Pat. No. 10,184,137

METHOD OF PRODUCING ISOPRENE MONOMER

AJINOMOTO CO., INC., Tok...

1. A host cell, comprising a first heterogeneous expression unit and a second heterogeneous expression unit, wherein:the first heterogenous expression unit comprises:
(a1) a polynucleotide encoding a mevalonate kinase derived from a microorganism belonging to a genus selected from the group consisting of Methanocella, Corynebacterium, Methanosaeta, and Nitrosopumilus; and
(b1) a promoter operatively linked to the polynucleotide; and
the second heterogenous expression unit comprises:
(a2) polynucleotide encoding an enzyme involved in a methylerythritol phosphate pathway; and
(b2) a promoter operatively linked to the polynucleotide.

US Pat. No. 10,226,044

AGRICULTURAL AND HORTICULTURAL COMPOSITION AND METHOD FOR CULTIVATING PLANT

AJINOMOTO CO., INC., Tok...

1. A method for cultivating a plant, which comprises sprinkling a solution consisting of 0.5 to 75 mM of isoleucine on a leaf surface and/or a fruit of a fruit vegetable, a fruit tree, or a grain plant,wherein the sprinkling of the solution provides an effect selected from the group consisting of:
a) promotion of blooming,
b) promotion of the appearance of fruit,
c) improvement in the number of fruit produced,
d) promotion of changing in color of fruit,
e) promotion of carotenoid synthesis or anthocyanin synthesis, and
f) combinations thereof.
US Pat. No. 10,202,333

AQUEOUS SOLUTION CONTAINING BIS(N EPSILON LAUROYLLYSINE)DICARBOXYLIC ACID DIAMIDE AND/OR SALT THEREOF, AND METHOD FOR PRODUCING SAME

AJINOMOTO CO., INC., Tok...

1. A method of producing an aqueous solution, comprising at least one bis(N?-lauroyl lysine)dicarboxylic acid diamide and/or a salt thereof, and having a pH of 9 to 11,said method comprising:
(1) reacting N?-lauroyl lysine and/or a salt thereof with at least one dicarboxylic acid dichloride in a water solvent having a pH of 12 to 14, to obtain a first aqueous solution comprising at least one bis(N?-lauroyl lysine)dicarboxylic acid diamide and/or a salt thereof;
(2) adjusting the pH of said first aqueous solution to obtain a second aqueous solution having a pH of 7.5 to 8.5;
(3) adding N?-lauroyl lysine to said second aqueous solution and filtering the resulting mixture, to obtain a third aqueous solution; and
(4) adjusting the pH of said third aqueous solution to a value of pH 9 to 11.
US Pat. No. 10,175,228

METHOD OF USING TRANSMEMBRANE CHANNEL-LIKE PROTEIN 6 (TMC6) PROTEIN TO IDENTIFY SUBSTANCES AFFECTING SALTY TASTE

Ajinomoto Co., Inc., Chu...

1. A method for identifying a substance that affects salty taste, comprising:contacting a test substance with a transmembrane channel-like protein 6 (TMC6) protein;
measuring an action of said test substance on the TMC6 protein upon contact;
identifying said substance as a substance that affects salty taste on the basis of the action measured, wherein said action is binding of the test substance to the TMC6 protein, activation of the TMC6 protein by the test substance, or inactivation of the TMC6 protein by the test substance.
US Pat. No. 10,144,683

METHOD FOR COLLECTING ISOPRENE CONTAINED IN FERMENTED GAS, AND METHOD FOR PRODUCING PURIFIED ISOPRENE

Ajinomoto Co., Inc., Tok...

1. A method, comprising:contacting a fermented gas comprising isoprene with a porous adsorbent; and
desorbing isoprene adsorbed on the porous adsorbent at a temperature of 80° C. or less;
wherein:
the fermented gas is obtained by culturing a microorganism having an ability to produce isoprene;
desorbing isoprene comprises desorbing isoprene gas; and
the porous adsorbent is a hydrophobidized silica gel with an average pore size of 60 Angstroms or less.

US Pat. No. 10,117,820

COSMETIC COMPOSITION

AJINOMOTO CO., INC., Chu...

1. A cosmetic composition, comprising (A) and (B):(A) decanoylproline or a salt thereof; and
(B) at least one component selected from the group consisting of (a) and (b):
(a) a compound represented by formula (II) or a salt thereof:

wherein R2 is an alkyl group having 5 to 11 carbon atoms, an alkenyl group having 5 to 11 carbon atoms, an alkynyl group having 5 to 11 carbon atoms, or an alkoxy group having 5 to 11 carbon atoms; and
(b) a compound represented by formula (III) or a salt thereof:

wherein R3 and R4 are each independently hydrogen, a hydroxy group, or —OCH3.
US Pat. No. 10,195,127

COSMETIC COMPOSITION

AJINOMOTO CO., INC., Tok...

1. A cosmetic composition, comprising:(A) an N-acyl basic amino acid powder;
(B) an inorganic powder; and
(C) an oil material comprising silicone oil,
wherein:
said N-acyl basic amino acid powder consists of one or more N-medium-chain-acyl basic amino acids selected from the group consisting of N?-monohexanoyl lysine, N?-monooctanoyl lysine and N?-monodecanoyl lysine,
said N-acyl basic amino acid powder is present in an amount of 2.0 to 32.4 wt % based on the entire weight of said cosmetic composition,
said inorganic powder is present in an amount of 50.6 to 87.0 wt % based on the entire weight of said cosmetic composition,
said oil material is present in an amount of 1.0 to 47.4 wt % based on the entire weight of said cosmetic composition,
said N-acyl basic amino acid powder and said inorganic powder are present in a weight ratio (weight of said N-acyl basic amino acid powder:weight of said inorganic powder) of: 2.2:97.8 to 36.4:63.6, and
said N-acyl basic amino acid powder and said oil material are present in a weight ratio (weight of said N-acyl basic amino acid powder:weight of said oil material) of 15.4:84.6 to 74.7:25.3.

US Pat. No. 10,512,161

RESIN SHEET

Ajinomoto Co., Inc., Tok...

1. A resin sheet, comprising:a support; and
a resin composition layer being in contact on the support,
wherein
a ratio (B/A) of a tensile breaking strength B of a 10 ?m thickness cured product of the resin composition layer after a HAST test to a tensile breaking strength A of a 10 ?m thickness cured product of the resin composition layer is 0.65 or more and 1 or less.
US Pat. No. 10,390,553

FOOD CONTAINING HISTIDINE AND USE THEREOF

AJINOMOTO CO., INC., Tok...

1. A method of improving mental energy, which comprises administering not less than 0.3 g of histidine as an ingestion amount per meal to a human subject in need thereof, wherein said subject exhibits a Pittsburgh Sleep Quality Index (PSQI) of not less than 6 and/or a factor T score of not less than 60 points in Profile of Mood Sates (POMS),wherein said histidine is administered in a liquid composition which comprises histidine in an amount not less than 3 w/v % wherein said subject is a human of 45 years old to less than 65 years old.
US Pat. No. 10,266,857

METHOD FOR PRODUCING N?-ACYL-L-LYSINE

AJINOMOTO CO., INC., Tok...

1. A method for producing N?-acyl-L-lysine comprising reacting a carboxylic acid or a salt thereof and L-lysine or a salt thereof in the presence of a protein to produce N?-acyl-L-lysine and collecting said N?-acyl-L-lysine, wherein the protein is selected from the group consisting of:(A) a protein comprising the amino acid sequence of SEQ ID NO: 1;
(B) a protein comprising the amino acid sequence of SEQ ID NO: 1, but in which no more than one to 50 amino acid residues are inserted, added, deleted, or substituted and wherein said protein has N?-acyl-L-lysine-specific aminoacylase activity; and
(C) a protein comprising an amino acid sequence having 90% or higher sequence identity with the amino acid sequence of SEQ ID NO: 1 and wherein said protein has N?-acyl-L-lysine-specific aminoacylase activity.
US Pat. No. 10,144,684

METHOD FOR COLLECTING ISOPRENOID COMPOUND CONTAINED IN FERMENTED GAS, AND METHOD FOR PRODUCING PURIFIED ISOPRENOID COMPOUND

Ajinomoto Co., Inc., Tok...

1. A method, comprising:contacting a fermented gas comprising an isoprenoid compound with a porous adsorbent; and
desorbing the isoprenoid compound adsorbed on the porous adsorbent at a temperature of 80° C. or less;
wherein:
the fermented gas is obtained by culturing a microorganism having an ability to produce the isoprenoid compound,
said desorbing said isoprenoid compound comprises desorbing isoprenoid gas, and
the porous adsorbent is a hydrophobidized silica gel with an average pore size of 60 Angstroms or less.

US Pat. No. 10,144,717

CYSTEINE DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. A cysteine compound represented by formula (I):
wherein
X and Y are each independently OR1 or NHR2 wherein R1 and R2 are each independently a hydrogen atom or a C1-22 alkyl group or X and Y together are —O—;
Z is a hydrogen or C1-22 alkyl group;
provided that when Z is a hydrogen atom, at least one of (i) and (ii) is satisfied;
(i) X is not methoxy, or
(ii) Y is not hydroxy; and
W is a C1-22 alkyl group, a C1-22 alkoxy group or a C1-22 alkylamino group,
or a salt thereof;
and provided that said compound or a salt thereof is not:
N-acetyl-2-methylthiazolidine-2,4-dicarboxylic acid or a salt thereof,
N-acetyl-2-methylthiazolidine-2,4-dicarboxylic acid anhydride or a salt thereof, or
N-acetyl-2-methylthiazolidine-2,4-dicarboxylic acid 2-ethyl ester or a salt thereof.
US Pat. No. 10,463,594

COSMETIC COMPOSITION

AJINOMOTO CO., INC., Tok...

1. A method for perming or coloring hair, comprising:treating hair with a cosmetic composition,
wherein the cosmetic composition comprises a protein-glutaminase, and the cosmetic composition is free of an alkaline substance and is free of a transglutaminase.

US Pat. No. 10,441,520

CLEANING AGENT COMPOSITION CONTAINING ACYL BASIC AMINO ACID DERIVATIVE

AJINOMOTO CO., INC., Tok...

1. A composition, comprising:(A) at least one compound represented by formula (1)
whereinR1 and R2 are each independently an alkyl group having 5 to 21 carbon atoms or an alkenyl group having 5-21 carbon atoms,
R3 and R4 are each independently a hydrogen atom, an alkyl group having 1 to 22 carbon atoms or an alkenyl group having 2 to 22 carbon atoms,
z 8,
x and y are each independently an integer of 2 to 4, or a salt thereof; and
(B) at least one kind of surfactant selected from the group consisting of an anionic surfactant having a carboxyl group, an amphoteric surfactant, and a nonionic surfactant.
US Pat. No. 10,442,834

DEPROTECTION METHOD

AJINOMOTO CO., INC., Tok...

1. A method of producing a sulfur-containing peptide, comprising:(a) reacting:
(i) a free amino group of an amino acid having a carboxy group protected by a protecting group or a first peptide having a C-terminus protected by a protecting group with
(ii) a free carboxy group of an amino acid having an amino group protected by a protecting group to obtain a second peptide; and
(b) deprotecting any C-terminus and N-terminus, which are protected by a protecting group, of said second peptide, to obtain said sulfur-containing peptide,
wherein said second peptide contains at least one functional group selected from the group consisting of a carboxy group, an amino group, and a hydroxy group, which is protected by a protecting group represented by formula (I):
R1—C(R2)(R3)-L1-  (I)wherein R1 is an aryl group optionally having one or more substituent(s), R2 and R3 are each, independently, a hydrogen atom or an aryl group optionally having one or more substituent(s), and L1 is a single bond, —O—CO— or —O—CH2—; andsaid second peptide contains at least one sulfur-containing amino acid residue selected from the group consisting of methionine, cysteine with protected sulfanyl group, and cysteine, and
wherein said method further comprises subjecting said second peptide to hydrogenation in the presence of a metal catalyst and a halogenated acetic acid.
US Pat. No. 10,392,638

METHOD FOR PRODUCING L-AMINO ACIDS USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE OVEREXPRESSING A GENE ENCODING AN IRON EXPORTER

AJINOMOTO CO., INC., Tok...

1. A method for producing an L-amino acid comprising:(i) cultivating an L-amino acid-producing bacterium of the family Enterobacteriaceae in a culture medium to produce and accumulate the L-amino acid in the culture medium, and
(ii) collecting the L-amino acid from the culture medium,
wherein said bacterium has been modified to overexpress a gene encoding an iron exporter compared to expression of said gene in the corresponding unmodified bacterium,
wherein said gene encoding an iron exporter is overexpressed by increasing the copy number of said gene, and/or modifying an expression regulatory region of said gene, so that the expression of said gene is enhanced as compared with the corresponding unmodified bacterium,
wherein said gene encoding an iron exporter encodes a protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence of SEQ ID NO: 2, 4 or 6;
(B) a protein comprising the amino acid sequence of SEQ ID NO: 2, 4 or 6, but which includes substitution, deletion, insertion, and/or addition of one to ten amino acid residues, wherein said protein has an iron exporter activity; and
(C) a protein comprising an amino acid sequence that is not less than 95% identical to the entire amino acid sequence of SEQ ID NO: 2, 4 or 6, wherein said protein has an iron exporter activity.
US Pat. No. 10,376,452

CLEANSING COMPOSITION

AJINOMOTO CO., INC., Tok...

1. A cleansing composition, comprising:(A) N-lauroylglycine or at least one salt thereof;
(B) N-myristoylglycine or at least one salt thereof; and
(C) at least one N-acyl acidic amino acid selected from the group consisting of lauroyl glutamic acid and cocoyl glutamic acid, or a salt thereof,
wherein a weight ratio of (A)/(B) is 0.3 to 3.5 and a weight ratio of ((A)+(B))/(C) is 4 to 9,
wherein said composition is a liquid.
US Pat. No. 10,364,405

TRANSPARENT SOLID DETERGENT

AJINOMOTO CO., INC., Tok...

1. A transparent solid detergent, comprising:(A) at least one N-acyl glutamic acid salt, wherein said at least one N-acyl glutamic acid salt comprises N-lauroylglutamic acid salt, myristoylglutamic acid salt, and cocoylglutamic acid salt;
(B) at least one acylglycine salt, wherein said at least one acylglycine salt comprises cocoylglycine salt;
(C) at least one polyvalent alcohol, wherein said at least one polyvalent alcohol comprises glycerol; and
(D) at least one lower alcohol,
wherein
said (A) at least one N-acyl glutamic acid salt is present in an amount of 35 to 45 parts by weight per 1 part by weight of said (B) at least one acylglycine salt;
said (C) at least one polyvalent alcohol is present in an amount of 11 to 24 parts by weight, per 1 part by weight of said (B) at least one acylglycine salt, and said (D) at least one lower alcohol is present in an amount of 2.5 to 30 parts by weight per 1 part of said (B) at least one acylglycine salt.
US Pat. No. 10,308,969

METHOD FOR PREPARING POLYMERIC PROTEIN COMPOSED OF MONOMERIC PROTEIN PRODUCED BY FUSING PROTEIN HAVING IMMUNOGLOBULIN FOLD STRUCTURE TO PROTEIN CAPABLE OF SERVING AS SUBUNIT STRUCTURE

AJINOMOTO CO., INC., Tok...

1. A method for producing a multimeric protein consisting of two or more monomeric protein, wherein said monomeric protein is obtained by fusing a protein having an immunoglobulin fold structure to either a protein or a functional fragment thereof that can serve as a subunit structure, the method comprising the steps of:(A) preparing a monomeric protein having an insoluble granular form in cells of a microorganism, the monomeric protein obtained by fusing a first protein having an immunoglobulin fold structure to a second different protein that can serve as a subunit structure;
(B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution comprising lauroyl glutamic acid and/or a salt thereof, resulting in a first solution;
(C) adding a phosphate buffer to the first solution obtained in step (B) so that the concentration of the lauroyl glutamic acid and/or salt thereof is 2 to 5%;
(D) diluting the solution obtained in step (C) in a buffer comprising arginine and/or an arginine derivative to lower the concentration of lauroyl glutamic acid and/or a salt thereof, resulting in a second solution; and
(E) replacing a solvent of the second solution obtained in step (C) with a buffer, resulting in removal said lauroyl glutamic acid and/or a salt thereof, using a method selected from the group consisting of:
(e1) column chromatography selected from the group consisting of gel filtration chromatography, ion-exchange chromatography, hydrophobic interaction chromatography, and combination thereof;
(e2) ultrafiltration;
(e3) dialysis; and
(e4) combinations thereof.

US Pat. No. 10,280,267

POLYALLYLAMINE DERIVATIVE

Ajinomoto Co., Inc., Tok...

1. A polyallylamine derivative, comprising 10 to 450 structural units represented by Formula (1):
wherein in Formula (1), A1 represents a group represented by Formula (2), Formula (3), Formula (4), or Formula (5), and a plurality of said A1s are optionally the same as or different from each other; with the proviso that at least one of said A1s is a group represented by Formula (4) or Formula (5):

wherein in Formulae (2) and (3), R1 represents an alkylene group optionally having a substituent, an alkenylene group optionally having a substituent, or an alkylene group having an ether bond optionally having a substituent; a represents an integer of 0 to 100; a plurality of said R1s are the same as or different from each other;
in Formulae (4) and (5), R1 represents an alkylene group optionally having a substituent, an alkenylene group optionally having a substituent, or alkylene group having an ether bond optionally having a substituent; R2 represents an alkyl group optionally having a substituent, an alkenyl group optionally having a substituent, or an alkyl group having an ether bond optionally having a substituent; a represents an integer of 0 to 100; and a plurality of said R1s are the same as or different from each other,
wherein NR2/(NR1+NR2)×100 is 1 to 35 where NR1 is an average number of moles of R1 present in the molecules of the polyallylamine derivative and NR2 is an average number of moles of R2 present in the molecules of the polyallylamine derivative.

US Pat. No. 10,472,624

MORPHOLINO OLIGONUCLEOTIDE MANUFACTURING METHOD

AJINOMOTO CO., INC., Tok...

1. A morpholino nucleotide represented by formula (I):
wherein
m is any integer of not less than 0,
P1 is a hydrogen atom, or a temporary protecting group removable under acidic conditions,
P2 is a protecting group having an alkyl group having not less than 10 and not more than 300 carbon atoms and/or an alkenyl group having not less than 10 and not more than 300 carbon atoms or a protecting group removable under conditions different from those for P1,
Base1 is a nucleic acid base optionally protected by a protecting group,
Base2 in the number of m are each independently a nucleic acid base optionally protected by a protecting group,
each X in the number of m is independently a di C1-6 alkylamino group, or a 1-piperazinyl group wherein a nitrogen atom at the 4-position is protected by a protecting group and further optionally substituted, and
W in the number of m are each an oxygen atom,
provided that:
1) at least one of said protecting group when Base1 is protected by a protecting group, any protecting group when any Base2 in the number of m are protected by a protecting group, and a protecting group for P2 is a protecting group having an alkyl group having not less than 10 and not more than 300 carbon atoms and/or an alkenyl group having not less than 10 and not more than 300 carbon atoms, and
2) when P2 is a protecting group having a linear alkyl group having not less than 10 and not more than 300 carbon atoms and/or a linear alkenyl group having not less than 10 and not more than 300 carbon atoms, at least one of Base1 and any Base2 in the number of m is a nucleic acid base protected by a protecting group having an alkyl group having not less than 10 and not more than 300 carbon atoms and/or an alkenyl group having not less than 10 and not more than 300 carbon atoms.
US Pat. No. 10,464,966

PRECIPITATION PROMOTER AND PRECIPITATION METHOD IN WHICH SAME IS USED

AJINOMOTO CO., INC., Tok...

1. A method of precipitating an organic compound protected by an organic group having one or more aliphatic hydrocarbon groups having not less than 10 carbon atoms from a solvent, said method comprising:mixing a precipitation promoter, said organic compound protected by an organic group, and said solvent, wherein
said precipitation promoter has one or more linear aliphatic hydrocarbon groups having not less than 10 carbon atoms, and
the aliphatic hydrocarbon group in the precipitation promoter has not less than 20 carbon atoms in total, and
said organic compound protected by an organic group is a nucleoside, nucleotide, or oligonucleotide optionally further protected by a protecting group used in nucleic acid synthesis, or an amino acid or peptide optionally further protected by a protecting group used in peptide synthesis.
US Pat. No. 10,415,036

MORPHOLINO OLIGONUCLEOTIDE MANUFACTURING METHOD

AJINOMOTO CO., INC., Tok...

1. A method of producing an n+p-mer morpholino oligonucleotide, comprising:(1) condensing (i) either (i?) a morpholino nucleoside or (i?) a p+1-mer morpholino oligonucleotide, wherein p is any integer of one or more, and wherein a 5?-hydroxy group is activated (thio)phosphated or activated (thio)phosphoramidated, and a morpholine ring nitrogen atom is protected by a temporary protecting group removable under acidic conditions, with (ii) either (ii?) a morpholino nucleoside or (ii?) an n+1-mer morpholino oligonucleotide, wherein n is an integer of one or more, and wherein a 5?-hydroxy group or, when the 5?-hydroxy group has a substituent having a hydroxy group, the hydroxy group present on the substituent is protected by a protecting group having an alkyl group containing not less than 10 and not more than 300 carbon atoms and/or an alkenyl group containing not less than 10 and not more than 300 carbon atoms, and the morpholine ring nitrogen atom is not protected, by a (thio)phosphoramidate bond or (thio)phosphorodiamidate bond via the morpholine ring nitrogen atom,
to obtain a reaction mixture comprising said n+p-mer morpholino oligonucleotide.
US Pat. No. 10,406,086

MOISTURIZER AND COSMETIC INCLUDING THE SAME

Ajinomoto Co., Inc., Tok...

1. A moisturizer, comprising at least one acyl neutral amino acid having an acyl group having a carbon atom number of 2 to 10 or a carbon number of 12 or a salt thereof.

US Pat. No. 10,385,185

SEALING RESIN COMPOSITION AND SEALING SHEET

AJINOMOTO CO., INC., Tok...

1. A sealing resin composition, comprising:(A) at least one epoxy resin;
(B) calcined hydrotalcite;
(C) talc; and
(D) silica,
wherein, based on 80 parts by mass of (A) said at least one epoxy resin:
(B) said calcined hydrotalcite is present in an amount of 5 to 38 parts by mass;
(C) said talc is present in an amount of 2 to 16 parts by mass; and
(D) said silica is present in an amount of 6 to 16 parts by mass,
which affords a cured product of thickness of 20 ?m having a transmittance of not less than 84% at 450 nm when the resin composition is heat hardened at 110° C. for 30 min.
US Pat. No. 10,369,163

ANTI-FATIGUE COMPOSITION

AJINOMOTO CO., INC., Tok...

1. An anti-fatigue composition, comprising (1) histidine and (2) vitamin B6 in combination,wherein:
said composition is contained in a unit package;
said histidine is present in said unit package in an amount of not less than 0.5 g per said unit package; and
said vitamin B6 is present in said unit package in an amount of not less than 15 mg per said unit package and said histidine and said vitamin B6 are present in a histidine:vitamin B6 weight ratio of about 120:1.
US Pat. No. 10,322,078

COSMETIC COMPOSITION CONTAINING 3-O-ALKYL-L-ASCORBIC ACID OR SALT THEREOF

AJINOMOTO CO., INC., Tok...

1. A cosmetic composition, comprising:(A) at least one 3-O-alkyl-L-ascorbic acid, wherein the alkyl group has 8 to 12 carbon atoms or a salt thereof;
B) at least one nonionic surfactant selected from the group consisting of a polyoxyethylene polyoxypropylene alkyl ether, a polyoxyethylene sorbitan fatty acid ester, a polyoxyethylene alkyl ether, and a polyoxyethylene alkenyl ether, and having an HLB value of 12.5 to 17; and
(C) at least one oil agent selected from the group consisting of an amino acid ester, a dimer acid ester, a hydroxy acid ester, an alkyl glyceryl ether, and alkenyl glyceryl ether.
US Pat. No. 10,450,407

COATED PARTICLES

Ajinomoto Co., Inc., Chu...

1. An epoxy resin composition comprising:(i) coated particles comprising:
(A) curing agent particles for epoxy resin; and
(B) an alkoxy oligomer,
wherein the surfaces of said curing agent particles for epoxy resin are coated with said alkoxy oligomer; and
(ii) an epoxy resin.
US Pat. No. 10,358,638

METHOD OF PRODUCING 1,5-PENTADIAMINE USING LYSINE DECARBOXYLASE MUTANT HAVING IMPROVED THERMAL STABILITY

AJINOMOTO CO., INC., Tok...

1. A method of producing 1,5-pentamethylenediamine, comprising allowing a lysine decarboxylase mutant to act on L-lysine and/or a salt thereof, wherein said lysine decarboxylase mutant has an amino acid sequence consisting essentially of the amino acid sequence of SEQ ID NO:1; and contains one of the following:(i) a substitution of Val at position 3, or
(ii) a substitution of Val at position 3 and a substitution of Ala at position 590,
wherein said lysine decarboxylase mutant maintains the lysine decarboxylation activity of a lysine decarboxylase having an amino acid sequence consisting essentially of SEQ ID NO:1 and has improved thermal stability as compared to a lysine decarboxylase having an amino acid sequence consisting essentially of SEQ ID NO:1.
US Pat. No. 10,508,295

GAMMA GLUTAMYL-VALINE SYNTHASE, AND METHOD FOR PRODUCING GAMMA GLUTAMYL-VALYL-GLYCINE

AJINOMOTO CO., INC., Tok...

1. A method for producing ?-Glu-Val-Gly and/or a salt thereof, the method comprising:contacting a protein and glutathione synthetase with Glu, Val, and Gly to produce ?-Glu-Val-Gly; and
collecting the produced ?-Glu-Val-Gly,
wherein the protein comprises an amino acid sequence having at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 6, 10, or 16, and has ?-glutamylvaline synthetase activity, and
a ratio of ?-glutamylvaline synthetase activity of the protein to ?-glutamylglycine synthetase activity of the protein is 15 or higher.
US Pat. No. 10,457,911

MEDIUM FOR STEM CELL USE

AJINOMOTO CO., INC., Tok...

1. A medium for culturing an iPS cell, comprising:polyvinyl alcohol; and
an albumin,
wherein the albumin and the polyvinyl alcohol are included in the medium in a weight ratio of 1:1.1 to 100, and
an amount of fatty acid binding to the albumin is not more than 2.2 mg per 1 g of the albumin.
US Pat. No. 10,428,359

METHOD FOR PRODUCING L-AMINO ACID

AJINOMOTO CO, INC., Toky...

1. A method for producing an L-amino acid, the method comprising the steps of:A) culturing a bacterium having an L-amino acid-producing ability in a medium resulting in accumulation of an L-amino acid in the medium; and
B) collecting the L-amino acid from the medium;
wherein the bacterium has been modified so that the activity of a C4-dicarboxylic acid-uptake carrier is increased as compared with a non-modified bacterium,
wherein the C4-dicarboxylic acid-uptake carrier is a protein encoded by a gene selected from the group consisting of a dctA gene, a dcuA gene, a dcuB gene, and combinations thereof, and
wherein the L-amino acid is an L-amino acid other than L-aspartic acid,
provided that the L-amino acid is L-glutamic acid when the C4-dicarboxylic acid-uptake carrier is a protein encoded by a dctA gene, and
provided that the bacterium has further been modified so as to harbor a mutant yggB gene when the bacterium is a coryneform bacterium and the C4-dicarboxylic acid-uptake carrier is a protein encoded by a dctA gene.