US Pat. No. 9,067,961

THIOCARBON-PROTECTING GROUPS FOR RNA SYNTHESIS

Agilent Technologies, Inc...

1. A compound having the structure of formula (II):
wherein:
BP is a protected or unprotected heterocyclic group having a 3 to 20 membered ring system;

one of R1 and R2 is a protecting group, and the other of R1 and R2 is a phosphoramidite group;

W is —NH2, —NH—R4 or —NR4—R5, —NH—O—R4 or —NR4—O—R5, wherein R4 and R5 are independently selected from a hydrocarbyl, a substituted hydrocarbyl, an aryl or a substituted aryl, wherein R4 and R5 are optionally cyclically linked, provided that W is not imidazole.

US Pat. No. 9,222,876

LIGHT SCATTERING FLOW CELL DEVICE

AGILENT TECHNOLOGIES, INC...

1. A flow cell device, comprising:
a solid flow cell body constructed entirely of an optically transmitting material and comprising an outer surface and a hollow
bore oriented along a central axis, the outer surface comprising:

a circular liquid inlet surface orthogonal to the central axis;
a circular liquid outlet surface orthogonal to the central axis and parallel to the liquid inlet surface; and
a sidewall extending between the liquid inlet surface and the liquid outlet surface and coaxially surrounding the hollow bore,
wherein the hollow bore comprises a liquid inlet end on the central axis at the liquid inlet surface and a liquid outlet end
on the central axis at the liquid outlet surface;

a liquid inlet structure facing the liquid inlet surface and comprising a first protruding section contacting the liquid inlet
end and extending through the liquid inlet end into the hollow bore, the liquid inlet structure having a liquid inlet port
formed in the first protruding section and fluidly communicating with the hollow bore; and

a liquid outlet structure facing the liquid outlet surface and comprising a second protruding section contacting the liquid
outlet end and extending through the liquid outlet end into the hollow bore, the liquid outlet structure having a liquid outlet
port formed in the second protruding section and fluidly communicating with the hollow bore.

US Pat. No. 9,151,738

SOLVENT EXTRACTION USING ENVIRONMENTALLY-FRIENDLY SILOXANE SOLVENTS

Agilent Technologies, Inc...

1. A method for performing solvent extraction to quantify contamination of a sample by a hydrocarbon contaminant, the method
comprising:
providing a defined quantity of the sample;
providing a defined quantity of a siloxane solvent;
mixing the defined quantity of the sample and the defined quantity of the siloxane solvent to extract the hydrocarbon contaminant
from the sample to form a contaminant solution with the siloxane solvent;

separating the contaminant solution from the sample; and
subjecting at least a portion of the separated contaminant solution to one of: molecular spectroscopic analysis; or gravimetric
analysis; or nuclear magnetic resonance (NMR) spectroscopic analysis.

US Pat. No. 9,345,121

WAVEGUIDE-BASED APPARATUS FOR EXCITING AND SUSTAINING A PLASMA

Agilent Technologies, Inc...

1. An apparatus, comprising:
an electromagnetic waveguide comprising a first end, a second end spaced from the first end along a first direction, and an
interior region between the first end and the second end, wherein the electromagnetic waveguide is configured to support propagation
of an electromagnetic wave in the first direction between the first end and the second end;

a first wall having a first aperture;
a second wall opposite to the first wall and having a second aperture, wherein the first wall and the second wall at least
partially define the interior region along a second direction nonparallel with the first direction; and

an electromagnetic-field shaping structure within the electromagnetic waveguide, the electromagnetic-field shaping structure
defining a channel therein extending from the first aperture to the second aperture, the channel having an axis extending
in the second direction,

wherein a distance between the first aperture and the second aperture in the second direction is less than a width of the
interior region in the second direction both at the first end and at the second end.

US Pat. No. 9,482,649

FITTING FOR ELASTICALLY-BIASING A CAPILLARY FOR A FLUID-TIGHT CONNECTION TO A FLUIDIC CONDUIT

Agilent Technologies, Inc...

1. A fitting for providing a fluid connection between a capillary and a fluidic conduit of a fluidic component, the fitting
comprising:
a capillary;
a sleeve fixedly fastened to the capillary and comprising an annular flange face surrounding the capillary; and
a male piece configured for connection with a female piece, the male piece comprising:
a housing with a capillary reception configured for receiving the capillary, wherein a part of the capillary being received
in the capillary reception is circumferentially covered by the sleeve, and the annular flange face faces the capillary reception;

an elastic biasing mechanism being arranged at least partially within the housing, being configured for biasing the capillary
against the female piece and being supported by the sleeve; and

a locking mechanism being arranged at least partially within the housing and being configured for locking the capillary to
the fitting at the annular flange face.

US Pat. No. 9,449,805

ISOLATION OF CHARGED PARTICLE OPTICS FROM VACUUM CHAMBER DEFORMATIONS

Agilent Technologies Inc....

1. A charged particle processing apparatus, comprising:
a vacuum chamber comprising a chamber wall;
an optics plate;
charged particle optics mounted to the optics plate; and
a plurality of mounting members coupled between the optics plate and the chamber wall, wherein the mounting members are configured
for cooperatively constraining six degrees of freedom of the optics plate, and at least one of the mounting members is movable
or flexible in at least one direction in response to deformation of the chamber wall.

US Pat. No. 9,449,804

DUAL FIELD MULTIPOLE CONVERGING ION GUIDES, HYPERBOLIC ION GUIDES, AND RELATED METHODS

Agilent Technologies, Inc...

1. An ion guide, comprising:
an entrance end;
an exit end at a distance from the entrance end along a guide axis;
a first RF field generator configured for generating a first RF field of Nth order where N is an integer equal to or greater
than 2, the first RF field generator comprising a plurality of first electrodes elongated along the guide axis and circumferentially
spaced about the guide axis, wherein the first electrodes surround a guide volume between the entrance end and the exit end;
and

a second RF field generator configured for generating a second RF field of 2Nth order superimposed on the first RF field and
penetrating between the first electrodes, the second RF field generator comprising one or more second electrodes.

US Pat. No. 9,506,897

METHODS AND COMPOSITIONS FOR IMPROVED ION-EXCHANGE CHROMATOGRAPHY

Agilent Technologies, Inc...

1. A method for analyzing or purifying one or more ionic analytes in a sample by anion-exchange chromatography, comprising:
providing an anion-exchange column packed with an anion-exchange stationary phase;
providing an anion-exchange mobile phase having a modifying agent;
performing anion-exchange chromatography under a condition allowing anion exchange between the one or more ionic analytes
in the sample and the anion-exchange stationary phase, thereby separating the one or more ionic analytes in the anion-exchange
column; and

analyzing and/or collecting the separated one or more ionic analytes;
wherein:
the modifying agent is an alkylene oxide oligomer comprising at least three units of an alkylene oxide moiety; and
at least one of the one or more ionic analytes comprises a polyethylene-glycol moiety.

US Pat. No. 9,297,807

PROTEIN ANALYSIS USING A POLYMETHINE MARKER DYE

Agilent Technologies, Inc...

1. A method of analyzing lipoproteins in a sample, the method comprising:
labeling the lipoproteins in a sample with a marker dye containing a polymethine of the general formula I

wherein Z has the general formula IIa

and wherein
X is C(CH3)2,

n is 1,
R1 is —C(CH3)3;

R2, R3, R4, R6, R7, R8, R9, R10, R11, R12 and/or R13 is hydrogen;

R5 is —N(CH2CH3)2;

R14 is —(CH2)3—OH, —(CH2)5—COOH, or —CH3;

performing on a microfluidic device a separation of the labeled lipoproteins in the sample; and
optically detecting the separated labeled lipoproteins.

US Pat. No. 9,354,208

APPARATUS FOR GENERATING SMALL FLOW RATES IN A CHANNEL

Agilent Technologies, Inc...

1. A fluidic apparatus for a sample separation system, the fluidic apparatus comprising
a fluid inlet path for supplying a fluid,
an inlet sensor adapted for measuring a flow rate of the fluid in the fluid inlet path,
a splitting point arranged downstream from the fluid inlet path and adapted for splitting the fluid into a first path and
into a second path, and

a second path sensor adapted for measuring a flow rate of a part of the fluid conducted into the second path,
wherein the fluid inlet path comprises a first inlet conduit for supplying a first fluidic component of the fluid, a second
inlet conduit for supplying a second fluidic component of the fluid, and a mixing point for mixing the first fluidic component
with the second fluidic component upstream from the splitting point.

US Pat. No. 9,053,917

VACUUM FIRED AND BRAZED ION PUMP ELEMENT

Agilent Technologies, Inc...

1. A Vacuum Fired and Brazed (“VFB”) conduit anode element for use in an ion pump, the VFB conduit anode element comprising;
a conduit comprising a sheet of metal surrounding a longitudinal axis of the conduit, the sheet comprising a first edge and
a second edge facing each other to define a joint line extending along an elongated side of the conduit in parallel with the
longitudinal axis; and

a brazing seal disposed between and contacting the first edge and the second edge along the joint line, the brazing seal comprising
a brazing alloy,

wherein the first edge and second edge are brazed together utilizing a vacuum brazing process.

US Pat. No. 9,330,889

PLASMA GENERATION DEVICE WITH MICROSTRIP RESONATOR

Agilent Technologies Inc....

1. A plasma generation device, comprising:
a substrate having a first surface and a second surface;
a resonant ring-shaped structure disposed over the first surface of the substrate, the resonant ring-shaped structure having
dimensions selected to support at least one standing wave having more than one electric field maximum along a length of the
resonant ring-shaped structure;

a ground plane disposed on the second surface of the substrate; and
an apparatus configured to provide a gas at locations of the electric field maxima,
wherein when the resonant ring-shaped structure comprises a gap, electrodes on both sides of the gap are at equipotential.
US Pat. No. 9,284,456

SUPERFICIALLY POROUS METAL OXIDE PARTICLES, METHODS FOR MAKING THEM, AND SEPARATION DEVICES USING THEM

Agilent Technologies, Inc...

1. Superficially porous particles, comprising:
solid cores each comprising an inorganic material or a hybrid material, said hybrid material including an inorganic material
and an organic material; and

porous outer shells each comprising the inorganic material or the hybrid material, and having ordered pores, wherein the ordered
pores have a median pore size in a range from about 15 to about 1000 Å and produce at least one X-ray diffraction peak between
0.01° and 10° of a 2? scan range, and wherein the ordered pores are elongated pores aligned substantially in a radial direction;

wherein the particles have a median size range from about 0.5 ?m to about 100 ?m, and
wherein the inorganic material comprises a metal oxide selected from silica, alumina, titania or zirconia.
US Pat. No. 9,181,534

HIGH FIDELITY DNA POLYMERASE COMPOSITIONS AND USES THEREOF

Agilent Technologies Inc....

1. An enzyme mixture comprising a first enzyme and a second enzyme, wherein said first enzyme is a DNA polymerase or reverse
transcriptase, and said second enzyme is the wild type Pfu DNA polymerase comprising the amino acid sequence of SEQ ID NO.
12, except that it is mutated at an amino acid position selected from the group consisting of: Y410, D543, K593, G387, G388,
and the following amino acid substitutions: Y385N, Y385W, Y385L, Y385H, Y385Q, and Y385S, and wherein said second enzyme possesses
reduced 3?-5? exonuclease activity and reduced 5?-3? DNA polymerization activity as compared to the wild type Pfu DNA polymerase.

US Pat. No. 9,291,627

ANALYTE DETECTION ASSAYS

Agilent Technologies Inc....

1. A composition comprising:
a modified polynucleotide template having one or more modified nucleotides and a 3? terminal nucleotide;
a binding molecule coupled to a first polymerase which amplifies the modified polynucleotide template; and
a second polymerase uncapable of amplifying the modified polynucleotide template but capable of amplifying an unmodified copy
of the modified polynucleotide template.

US Pat. No. 9,482,642

FAST METHOD FOR MEASURING COLLISION CROSS SECTION OF IONS UTILIZING ION MOBILITY SPECTROMETRY

Agilent Technologies, Inc...

18. An ion mobility-mass spectrometry (IM-MS) system, comprising:
an IM drift cell comprising a drift cell entrance and a drift cell exit;
an intermediate region following the drift cell exit and comprising a mobility-dependent region;
an ion detector following the intermediate region; and
a computing device configured for measuring the collision cross section (CCS) of a sample ion by:
measuring a total drift time taken by the sample ion to travel from the drift cell entrance to the ion detector; and
calculating the CCS of the sample ion from the following equation:
?=(tD?tfix)/??,

wherein ? is the CCS, tD is the total drift time measured, tfix is a fixed delay time parameter, ? is a proportionality coefficient relating to a drift time taken by the sample ion to travel
through the drift cell and through a mobility-dependent region outside the drift cell, and ?=(mI/mI+mB))1/2/q, wherein mI is the mass of the sample ion, mB is the mass of a drift gas molecule in the drift cell, and q is the charge state of the sample ion.

US Pat. No. 9,114,330

SYSTEM AND PROCESS FOR AN ACTIVE DRAIN FOR GAS-LIQUID SEPARATORS

Agilent Technologies, Inc...

1. A system, comprising:
a chromatographic system comprising a separation column configured to elute solutes of samples injected into a flow stream;
a gas-liquid separator configured to receive and separate a pressurized biphasic flow stream from the separation column and
to deliver flow to at least one exit port enriched in liquid phase composition;

a low restriction inlet conduit in communication with the at least one separator exit port enriched in liquid phase composition;
a positive displacement pump comprising an inlet port in communication with the inlet conduit and an outlet port in communication
with an outlet conduit; and

a collection system connected to the outlet conduit.

US Pat. No. 9,075,068

INTEGRATED PARTICLE TRAP FOR CAPILLARY PLOT COLUMNS

Agilent Technologies, Inc...

18. A method for preparing a porous layer open tubular (PLOT) column having two integrated particle traps disposed at the
two end sections, comprising:
stripping a first section of a stationary phase coating from a first end of a PLOT column;
preparing a first particle trap in the stripped first section;
stripping a second section of a stationary phase coating from a second end of a PLOT column; and
preparing a second particle trap in the stripped second section,wherein there is no connector between the capillary tubing and either of the two integrated particle traps.

US Pat. No. 9,081,044

DETECTION CELL WITH CONTROLLED POWER DISSIPATION

Agilent Technologies, Inc...

1. Control circuitry configured for determining an electrical property of a sample in a detection cell volume of a detection
cell, in order to detect and characterize a compound in the sample, the control circuitry comprising:
a power supply configured for providing at least one of a voltage and a current to the detection cell;
a power evaluation unit configured for determining an actual power dissipation in the detection cell volume based on a product
of a current passing through the detection cell volume and one of the voltage provided by the power supply or a control voltage,
wherein the detection cell volume is part of a detection flow path, with a fluid sample passing through the detection cell
volume;

a control unit configured for comparing the actual power dissipation with a desired power dissipation, and for regulating
at least one of the voltage and the current provided by the power supply to drive the actual power dissipation towards the
desired power dissipation; and

a divider unit configured for determining a quotient of the current passing through the detection cell volume and one of the
voltage provided by the power supply or the control voltage, for deriving the electrical property of the sample.

US Pat. No. 9,228,982

SINGLE INJECTION VALVE FOR HPLC COMBINING SAMPLE INTRODUCTION, WASH CYCLES AND DIAGNOSIS

AGILENT TECHNOLOGIES, INC...

1. A sample injector configured to introduce a sample fluid into a mobile phase, wherein the mobile phase is to be driven
by a mobile phase drive through a separation unit for separating compounds of the sample fluid in the mobile phase, the sample
injector comprising:
a sample loop for receiving the sample fluid,
a switchable valve for switching the sample loop between the mobile phase drive and the separation unit, wherein the switchable
valve comprises;

a first valve member and a second valve member, wherein at least one of the first and second valve members is configured to
be moved with respect to the other;

the first valve member comprises a plurality of ports;
the second valve member comprises a plurality of fluid paths for fluidly coupling respective ones of the ports in dependency
on a relative movement position of the first and second valve members with respect to each other;

wherein the plurality of ports comprise eight circumferential ports distributed along a circumference of the first valve member
and comprise one central port arranged at a central position of the first valve member;

wherein one of the circumferential ports has an extension groove; wherein the plurality of fluid paths comprise three arcuate
fluid paths each being arcuate so as to be couplable with at least two of the circumferential ports;

wherein the plurality of fluid paths comprise one straight fluid path extending between the central position and a circumferential
position so as to be couplable with the central port and with one of the circumferential ports.

US Pat. No. 9,105,454

PLASMA-BASED ELECTRON CAPTURE DISSOCIATION (ECD) APPARATUS AND RELATED SYSTEMS AND METHODS

Agilent Technologies, Inc...

1. An electron capture dissociation (ECD) apparatus, comprising:
a plasma source configured for generating plasma;
a plasma refinement device configured for converting the generated plasma to refined plasma comprising predominantly low-energy
electrons suitable for ECD and plasma ions; and

a chamber configured for receiving an ion beam in an interaction region containing the refined plasma.

US Pat. No. 9,053,915

RADIO FREQUENCY (RF) ION GUIDE FOR IMPROVED PERFORMANCE IN MASS SPECTROMETERS AT HIGH PRESSURE

Agilent Technologies, Inc...

1. A mass spectrometer having an inlet that is maintained, at a first pressure and a region that is maintained at a second
pressure that is less than the first pressure, the inlet configured to receive an ion guide, wherein the ion guide comprises:
a substrate comprising a plurality of electrodes disposed thereover, the substrate forming a first opening at a first end
and a second opening at a second end, wherein the first opening is configured to receive ions at the first pressure;

a plurality of trenches provided in the substrate, wherein each of the plurality of trenches is provided between a respective
adjacent pair of the plurality of electrodes;

means for applying a radio frequency (RF) voltage between adjacent pairs of the plurality of electrodes, wherein the RF voltage
creates a field in a region defined by the substrate; and

means for applying a direct current (DC) voltage drop along a length of each of the plurality of electrodes.

US Pat. No. 9,275,841

TIME OF FLIGHT MASS SPECTROMETER UTILIZING OVERLAPPING FRAMES

Agilent Technologies, Inc...

1. A spectrometer comprising:
an event extractor configured to generate an ion event record corresponding to each ion satisfying a detection criterion detected
by an ion detector, said ion event record including an intensity and one or more aliases for that detected ion; and

a streaming processor configured to receive said ion event records, store a time-of-flight (TOF) spectrum, and update that
TOF spectrum for each alias in each of said received ion event records, said updates depending on a probability for each alias
in each received ion event record and said intensity in that ion event record, said streaming processor storing said received
ion event records in a buffer,

wherein each received ion event record comprises a timestamp determined by a time said received ion event record was received
by said streaming processor.

US Pat. No. 9,265,138

ELECTROMAGNETIC WAVEGUIDE AND PLASMA SOURCE

Agilent Technologies, Inc...

1. An apparatus, comprising:
an electromagnetic waveguide having a length and height;
a first iris slot crossing the waveguide at a first position along the length of the waveguide having a height less than that
of the waveguide;

a second iris slot crossing the waveguide at a second position along the length of the waveguide having a height less than
that of the waveguide; and

a plasma torch having a longitudinal axis crossing the waveguide at a position between the first and second iris slots;
wherein the first iris slot and the second iris slot are configured to transmit electromagnetic fields substantially transverse
to the longitudinal axis of the plasma torch to excite plasma in the plasma torch; and

wherein the heights of the first iris slot and the second iris slot are less than 70% of the diameter of the torch.

US Pat. No. 9,243,891

METHODS AND APPARATUS FOR ACQUIRING PHYSICAL MEASUREMENTS RELATING TO A VESSEL AND A SHAFT WITHIN A VESSEL

AGILENT TECHNOLOGIES, INC...

1. An apparatus for determining at least one parameter relating to a position of an elongate member disposed within a vessel,
the apparatus comprising:
at least one measurement unit comprising a light emitting component and a corresponding light receiving component, the light
receiving component positioned apart from the light emitting component such that the elongate member is positionable between
the light emitting component and the light receiving component;

the light emitting component configured for emitting light towards the light receiving component; and
the light receiving component configured for generating a signal corresponding to the light received from the light emitting
component, the signal corresponding to light received from the light emitting component indicating the at least one parameter
relating to the position of the elongate member disposed within the vessel.

US Pat. No. 9,124,068

METHOD AND APPARATUS TO REDUCE NOISE CAUSED BY MODE HOPPING IN EXTERNAL CAVITY LASERS

Agilent Technologies, Inc...

1. An apparatus comprising:
a gain chip;
an optical frequency selective element that sends selectively attenuated light leaving said gain chip as a function of optical
frequency in a band of wavelengths back to said gain chip, said optical frequency selective element being characterized by
an angle relative to light leaving said gain chip and distance from said gain chip, said angle and said distance being controlled
by an actuator, a part of said light from the said optical frequency selective element being amplified by said gain chip;
and

an actuator driver that causes said angle to be dithered about an equilibrium angle in a motion characterized by an amplitude
and average frequency.

US Pat. No. 9,102,936

METHOD OF ADAPTOR-DIMER SUBTRACTION USING A CRISPR CAS6 PROTEIN

Agilent Technologies, Inc...

1. A method of processing a target RNA, comprising:
contacting the products of an RNA ligase-mediated ligation reaction with a CAS6 protein, wherein: (i) said RNA ligase-mediated
ligation reaction comprises: a target RNA, an RNA ligase, and first and second adaptors that can ligate together to produce
an adaptor dimer that contains a CRISPR stem loop; and (ii) said CAS6 protein recognizes said CRISPR stem loop;

thereby preventing said adaptor dimer from being reverse transcribed.

US Pat. No. 9,281,173

ION PROCESSING UTILIZING SEGMENTED VACUUM MANIFOLD

Agilent Technologies, Inc...

1. An ion processing device, comprising:
a vacuum manifold comprising a plurality of electrically conductive manifold segments serially positioned and enclosing a
volume along a longitudinal axis, the manifold segments electrically isolated from each other wherein each manifold segment
is independently addressable by a voltage source; and

an ion optics device positioned in the volume along the longitudinal axis and spaced from the vacuum manifold along a radial
direction orthogonal to the longitudinal axis, the ion optics device comprising an ion entrance and an ion exit and configured
for applying an axial voltage gradient from the ion entrance to the ion exit.

US Pat. No. 9,182,334

METHOD OF DETERMINING THERMODYNAMIC AND KINETIC PARAMETERS FROM MEASURED OFF RATES

Agilent Technologies, Inc...

1. A method for measuring a property of a binding interaction between a capture agent and a binding partner for said capture
agent, comprising:
a) contacting a population of particles that are linked to a capture agent with a substrate comprising a binding partner to
produce capture agent/binding partner complexes, wherein said population of particles comprises first particles that are bound
to a single molecule of said capture agent and second particles that are bound to two molecules of said capture agent;

b) applying a force to the particles, wherein said force is in a direction that separates said particles from said substrate;
c) separately measuring:
(i) the force required to disassociate the first particles from their respective complexes and
(ii) the force required to dissociate the second particles from their respective complexes;
d) calculating the off rates of said first particles and said second particles as a function of the applied force;
e) calculating the off rate for said first particles in the absence of applied force from the dependence of off rates on the
applied force; and

f) calculating the on rates for binding said first particles and said second particles, using the respective off rates calculated
in d) and e).

US Pat. No. 9,273,086

PROTECTED MONOMER AND METHOD OF FINAL DEPROTECTION FOR RNA SYNTHESIS

Agilent Technologies, Inc...

1. A method of deprotecting a solid support bound polynucleotide comprising at least one 2?-protected ribonucleotide residue
comprising the structure:
wherein:
BP is a protected or unprotected heterocycle;

R12 is a protecting group selected from a hydrocarbyl, a substituted hydrocarbyl, an aryl, and a substituted aryl;

X is O or S; and
PG is a thionocarbamate protecting group selected from one of the structures:
said method comprising:
contacting said polynucleotide with a composition comprising a diamine under conditions sufficient to deprotect said at least
one 2?-protected ribonucleotide residue.

US Pat. No. 9,337,008

ISOLATION OF CHARGED PARTICLE OPTICS FROM VACUUM CHAMBER DEFORMATIONS

Agilent Technologies, Inc...

1. A charged particle processing apparatus, comprising:
a vacuum chamber comprising a chamber wall;
an optics plate;
charged particle optics mounted to the optics plate; and
a plurality of mounting members coupled between the optics plate and the chamber wall, wherein the mounting members are configured
for cooperatively constraining six degrees of freedom of the optics plate, and at least one of the mounting members is movable
or flexible in at least one direction in response to deformation of the chamber wall.

US Pat. No. 9,247,629

WAVEGUIDE-BASED APPARATUS FOR EXCITING AND SUSTAINING A PLASMA

AGILENT TECHNOLOGIES, INC...

1. An apparatus, comprising:
an electromagnetic waveguide;
an iris structure providing an iris in the electromagnetic waveguide, the iris structure defining an iris hole, a first iris
slot at a first side of the iris hole, and a second iris slot at a second side of the iris hole;

a plasma torch disposed within the iris hole and comprising an outermost surface, wherein a gap is defined in the iris hole
between the outermost surface and the iris structure; and

a dielectric material disposed in the gap, outside of the plasma torch.
US Pat. No. 9,193,992

METHOD FOR DETERMINING PLOIDY OF A CELL

Agilent Technologies, Inc...

1. A method for determining the ploidy of a test genome, comprising:
a) obtaining a plurality of ratios for polymorphisms that are distributed throughout a test genome, wherein each of said ratios
is a ratio of the measured copy number of one allele of a polymorphism relative to the measured copy number of said allele
in a reference sample that has two copies of said allele;

b) plotting a distribution of the ratios to provide a distribution, wherein one axis of the distribution indicates a range
of ratios and another axis of the distribution represents a number of ratios, wherein the distribution comprises a number
of peaks; and

c) determining the ploidy of said test genome based on the number of peaks in said distribution.

US Pat. No. 9,236,232

MULTI-BORE CAPILLARY FOR MASS SPECTROMETER

AGILENT TECHNOLOGIES, INC...

1. A mass spectrometer system, comprising:
an ion source configured to produce ions; and
a capillary formed of a non-metallic material and configured to receive at least a portion of the ions from the ion source,
the capillary comprising an elongated body and a plurality of bores formed through the non-metallic material, traversing the
elongated body in a longitudinal direction from the ion source to a vacuum chamber of the mass spectrometer system, the plurality
of bores transporting the received ions through the capillary toward a mass analyzer for detection.

US Pat. No. 9,236,235

CURVED ION GUIDE AND RELATED METHODS

AGILENT TECHNOLOGIES, INC...

1. An ion guide comprising:
a plurality of curved electrodes arranged in parallel with each other and with a central curved axis, the curved central axis
being co-extensive with an arc of a circular section having a radius of curvature, each electrode being radially spaced from
the curved central axis, wherein the plurality of electrodes define a curved ion guide region arranged about the curved central
axis and between opposing pairs of the electrodes; and

an ion deflecting device configured for applying a radial DC electric field across the ion guide region and along the radius
of curvature.

US Pat. No. 9,234,892

MULTIPLE EPITOPE DETECTION IN AN FFPE TISSUE SECTION

Agilent Technologies, Inc...

1. A method for labelling a tissue section, comprising:
(a) labeling a formalin-fixed paraffin embedded (FFPE) tissue section using a first set of labeling reagents that comprises
a first primary antibody and a first labeled secondary antibody;

(b) treating the labeled tissue with a protease, thereby digesting the first primary antibody and/or the first labeled secondary
antibody and separating the label from the FFPE tissue section;

(c) washing the tissue section to remove the separated label and the protease; and
(d) labeling the FFPE tissue section using a second set of labeling reagents that comprises a second primary antibody and
a second labeled secondary antibody.

US Pat. No. 9,151,672

OPTICAL ABSORPTION SPECTROMETRY SYSTEM INCLUDING DICHROIC BEAM COMBINER AND SPLITTER

Agilent Technologies, Inc...

1. An optical absorption spectrometry system, comprising:
a first light source configured to emit first light having first wavelengths within a first wavelength range;
a second light source configured to emit second light having second wavelengths within a second wavelength range different
from the first wavelength range;

a dichroic beam combiner comprising a predetermined first reflectance/transmission transition region, the dichroic beam combiner
being configured to transmit a first portion of the first light and to reflect a second portion of the second light to provide
combined light; and

a wavelength selective module configured to disperse the combined light received at an entrance aperture, to select a sample
wavelength range of the dispersed light as sample light, and to output the sample light having the selected sample wavelength
range from an exit aperture for illuminating a sample.

US Pat. No. 9,455,132

ION MOBILITY SPECTROMETRY-MASS SPECTROMETRY (IMS-MS) WITH IMPROVED ION TRANSMISSION AND IMS RESOLUTION

Agilent Technologies, Inc...

1. An interface for an ion mobility spectrometry-mass spectrometry (IMS-MS) system, the interface comprising:
an ion mobility drift cell comprising a drift cell exit at which ions exit with a drift resolution;
a housing comprising a first chamber, an adjacent second chamber fluidly isolated from the first chamber, and a third chamber
downstream from the second chamber;

an ion funnel in the first chamber communicating with the drift cell exit, the ion funnel surrounding a funnel interior and
configured for generating a radial RF ion confining field in the funnel interior;

a first multipole ion guide in the second chamber, the first multipole ion guide comprising a plurality of guide electrodes
elongated along an axis, circumferentially spaced about the axis, and surrounding a guide interior, the guide electrodes each
comprising an insulating element and a resistive element surrounding the insulating element, and the guide electrodes configured
for generating a radial RF ion confining field in the guide interior and an axial DC gradient along a length of the first
multipole ion guide configured for maintaining the drift resolution of the ions, wherein the first multipole ion guide is
configured for operating at a pressure that avoids ion fragmentation;

a second multipole ion guide in the third chamber; and
an ion lens element positioned on the axis between the ion funnel and the first multipole ion guide.

US Pat. No. 9,328,730

ANGULAR SYNCHRONIZATION OF STATIONARY AND ORBITING PLATE SCROLL BLADES IN A SCROLL PUMP USING A METALLIC BELLOWS

Agilent Technologies, Inc...

1. The combination of pump head parts of a scroll pump and an appliance for use in assembling the pump head parts, the combination
comprising:
a frame;
an orbiting plate scroll having a front side and a back side, and comprising an orbiting scroll blade at its front side;
an annular metallic bellows having first and second ends;
a stationary plate scroll having a front side and a back side, and comprising a stationary scroll blade at its front side;
and

assembly parts by which the stationary and orbiting scroll blades are angularly synchronized with one another during assembly,
when the bellows is fixed to the orbiting plate scroll and to the frame, and the stationary plate scroll is fixed to the frame,

the assembly parts comprising a fixture having a reference feature, and fasteners that fasten the bellows at the first end
thereof to the orbiting plate scroll, the bellows at the second end thereof to the frame, and the stationary plate scroll
to the frame, respectively, and

wherein the fixture is configured so as to be mountable to an assemblage comprising the bellows and the orbiting plate scroll,
and

one of the orbiting plate scroll and the frame has another reference feature that is aligned with the reference feature of
the fixture, in the circumferential direction of the bellows.

US Pat. No. 9,200,885

INTERFEROMETER HAVING MULTIPLE SCAN CARRIAGES

Agilent Technologies, Inc...

1. An interferometer, comprising:
a first assembly comprising a base, a beam splitter assembly configured to split light emitted from a light source into first
and second portions of light, and a fixed mirror for reflecting the first portion of light;

a second assembly, movable with respect to the first assembly, comprising a first scan carriage, a second scan carriage and
a movable mirror connected to the second scan carriage for reflecting the second portion of light, wherein the beam splitter
assembly is further configured to combine the reflected first and second portions of light into a recombined radiation beam;

a pair of inner bearing flexures having ends connected to the base and ends connected to the first scan carriage, enabling
movement of the first scan carriage relative to the base; and

a pair of outer bearing flexures having ends connected to the first scan carriage and ends connected to the second scan carriage,
enabling movement of the second scan carriage relative to the first scan carriage,

wherein the movement of the first scan carriage and the movement of the second scan carriage enable a scan movement of the
movable mirror in a scan direction restricted such that the scan movement maintains a plane containing the movable mirror
parallel to a plurality of planes containing the movable mirror at respective distances between the second assembly and the
first assembly during the scan movement.

US Pat. No. 9,153,427

VACUUM ULTRAVIOLET PHOTON SOURCE, IONIZATION APPARATUS, AND RELATED METHODS

Agilent Technologies, Inc...

1. A vacuum ultraviolet (VUV) photon source, comprising:
a body enclosing an interior and comprising a VUV window, wherein the body is coaxially disposed about a longitudinal axis;
a first electrode disposed on the body outside the interior;
a dielectric barrier; and
a second electrode disposed on the dielectric barrier outside the interior, and separated from the first electrode by a gap
in the interior, wherein the first electrode and the second electrode are axially spaced from each other along the longitudinal
axis, and the dielectric barrier is disposed between the first electrode and the second electrode.

US Pat. No. 9,103,809

LIQUID HANDLER WITH DUAL PIPETTING GROUPS

Agilent Technologies, Inc...

1. A liquid handling system, comprising:
a first pipetting group, comprising at least one pipettor, movably arranged on a first arm, wherein each of the at least one
pipettor is independently movable in a Y-axis direction, which is parallel with a longitudinal axis of the first arm, and
in a Z-axis direction, which is perpendicular to the Y-axis direction; and

a second pipetting group, comprising at least one pipettor, movably arranged on a second arm, wherein each of the at least
one pipettor is independently movable in the Y-axis direction and in a Z-axis direction, wherein the first arm and the second
arm are movably arranged on at least one track such that the first arm and the second arm can independently move along the
at least one track in a X-axis direction, which is perpendicular to the Y-axis direction and the Z-axis direction, while keeping
the first arm parallel with the second arm, wherein the at least one pipettor of the first pipetting group is arranged on
a side of the first arm immediately facing the second pipetting group, wherein the at least one pipettor of the second pipetting
group is arranged on a side of the second arm immediately facing the first pipetting group.

US Pat. No. 9,404,491

SCROLL PUMP HAVING BELLOWS PROVIDING ANGULAR SYNCHRONIZATION AND BACK-UP SYSTEM FOR BELLOWS

Agilent Technologies, Inc...

1. A scroll pump comprising:
a frame;
a stationary plate scroll fixed to the frame and comprising a stationary scroll blade centered about a longitudinal axis of
the scroll pump, wherein the frame and the stationary plate scroll are a stationary part of the scroll pump;

an orbiting plate scroll comprising an orbiting scroll blade juxtaposed with the stationary scroll blade in a radial direction
of the scroll pump such that the stationary scroll blade and the orbiting scroll blade are nested;

an eccentric drive mechanism supported by the frame and operatively connected to the orbiting scroll blade so as to cause
the orbiting scroll blade to orbit about the longitudinal axis of the scroll pump, and wherein the eccentric drive mechanism
supports the orbiting scroll blade so as to be rotatable about a second axis that is parallel to the longitudinal axis of
the scroll pump, wherein the orbiting plate scroll is an orbital part of the scroll pump;

a metallic bellows comprising a first end and a second end, the metallic bellows being fixed at the second end to the frame,
and the orbiting scroll blade being fixed to the metallic bellows at the first end such that the metallic bellows angularly
synchronizes the orbiting scroll blade and the stationary scroll blade; and

a non-contacting back-up system comprising a plurality of pins extending axially in directions parallel to the longitudinal
axis of the scroll pump from one of the stationary and orbital parts of the scroll pump, and a guide part fixed relative to
the other of the stationary and orbital parts of the scroll pump, the guide part comprising a plurality of surfaces defining
a plurality of respective openings extending axially in the directions parallel to the longitudinal axis of the scroll pump,
wherein:

each of the plurality of pins extends axially into a respective one of the openings, and
each of the plurality of pins is spaced in its entirety from the other of the stationary and orbital parts of the scroll pump
such that a radial clearance exists between each pin of the plurality of pins and each respective surface.

US Pat. No. 9,182,325

METHOD AND APPARATUS FOR CHANGING RELATIVE CONCENTRATIONS OF GASES PRESENT WITHIN A GASEOUS SAMPLE FOR MASS SPECTROMETRY

Agilent Technologies, Inc...

1. An apparatus for changing relative concentrations of first and second analyte substances in an atmospheric analyte sample
to facilitate analysis of one of the analyte substances, the apparatus comprising:
a sample cell, defining therewithin a sample chamber;
a semipermeable boundary member disposed in the sample chamber to define first and second sides of the sample chamber;
a sample flow input duct for directing the atmospheric analyte sample containing respective initial concentrations of the
first and second analyte substances into the first side of the sample chamber;

a sample flow output duct for directing the atmospheric analyte sample containing respective final concentrations of the first
and second analyte substances out of the first side of the sample chamber; and

an analyzer connected to the sample flow output duct for analyzing the first and second analyte substances;
wherein the semipermeable boundary member permits migration therethrough of the first and second analyte substances to the
second side of the sample chamber at different rates so that the analyte sample exiting the first side via the sample flow
output duct has relative final concentrations of the first and second analyte substances that are different from the relative
initial concentrations;

wherein the apparatus is configured to direct an atmospheric sample comprising the initial concentrations of the first and
second analyte substances through the sample flow input duct and to analyze the final concentrations of the first and second
analyte substances that exit the first side via the sample flow output duct.

US Pat. No. 9,050,548

MULTI-CHANNEL FILTER ASSEMBLY AND RELATED APPARATUS AND METHODS

AGILENT TECHNOLOGIES, INC...

1. A filter assembly, comprising:
a housing comprising:
a first outside surface;
a second outside surface parallel to the first outside surface and spaced therefrom along a first axis;
a plurality of internal chambers between the first outside surface and the second outside surface, each internal chamber including
a first inside surface lying along a second axis perpendicular to the first axis, a second inside surface parallel to the
first inside surface, and a filter support extending from the second inside surface along the first axis, and each internal
chamber having a chamber height along the first axis and a chamber diameter along the second axis greater than the chamber
height;

a plurality of fluid inlet bores extending from the first outside surface to the respective first inside surfaces;
a plurality of fluid outlet bores extending from the respective second inside surfaces to the second outside surface; and
a lateral surface extending between the first outside surface and the second outside surface and defining a thickness of the
housing, wherein the first outside surface, the second outside surface, and the lateral surface are adjoined together such
that the housing has a unitary construction and the filter assembly is movable as a single unit; and

a plurality of filter elements disposed in the respective internal chambers in contact with the respective filter supports,
each filter element having a filter thickness along the first axis less than the chamber height, wherein:

each filter element partitions the respective internal chamber into a chamber inlet section between the first inside surface
and the filter element and a chamber outlet section between the filter element and second inside surface;

the housing establishes a plurality of fluid flow channels from the inlet bores, through the chamber inlet sections, through
the filter elements, through the chamber outlet sections and to the outlet bores, respectively; and

each fluid flow channel includes a transverse fluid flow component along the second axis in the chamber inlet section and
in the chamber outlet section.

US Pat. No. 9,117,617

AXIAL MAGNETIC ION SOURCE AND RELATED IONIZATION METHODS

Agilent Technologies, Inc...

1. An ion source, comprising:
a body comprising an ionization chamber and a sample inlet leading into the ionization chamber, the ionization chamber comprising
a first end and a second end, and having a length along a source axis from the first end to the second end;

a magnet assembly surrounding the body and configured for generating an axial magnetic field in the ionization chamber;
an electron source positioned at the first end and comprising a thermionic cathode and an electron reflector, the electron
source configured for accelerating an electron beam through the ionization chamber along the source axis; and

a lens assembly comprising an extractor positioned at the second end, a first lens element outside the ionization chamber
and spaced from the extractor along the source axis, and a second lens element spaced from the first lens element along the
source axis, wherein the extractor is configured for directing an ion beam out from the ionization chamber along the source
axis, the first lens element is configured for reflecting the electron beam toward the electron source, and the second lens
element is configured for transmitting higher energy ions while reflecting lower energy ions toward the first lens element.

US Pat. No. 9,134,335

CERAMIC INJECTION NEEDLE

Agilent Technologies, Inc...

1. A needle for handling a fluid in an analysis system, the needle comprising:
a needle body made of a ceramic material and having a fluid conduit extending between a fitting end and a seat end, the fitting
end being connectable to a fitting and the seat end is configured to be inserted into a seat, wherein the needle body is tapering
towards the fitting end;

a fixing body arranged on the needle body next to the fitting end for exerting an axial force when the needle body is connected
to the fitting, wherein the fixing body tapers towards the tapering of the needle body;

a slide-on element to be slid over the needle body so as to push the fixing body towards the fitting end; and
a springloading element-arranged over the needle body between the fixing body and the slide-on element.
US Pat. No. 9,169,286

USE OF N-ALKYL IMIDAZOLE FOR SULFURIZATION OF OLIGONUCLEOTIDES WITH AN ACETYL DISULFIDE

Agilent Technologies, Inc...

1. A method of sulfurizing at least one phosphite or thiophosphite linkage in an oligonucleotide, the method comprising:
contacting an oligonucleotide comprising a phosphite or thiophosphite intermediate with a composition comprising an acetyl
disulfide, a solvent, and an N-alkyl imidazole, for a time sufficient to convert the phosphite or thiophosphite intermediate
to a phosphorothioate or phosphorodithioate;

wherein the N-alkyl imidazole is at up to 25% (v/v) in the solvent.
US Pat. No. 9,074,203

LIGATION METHOD EMPLOYING RTCB

Agilent Technologies, Inc...

1. A method of processing a heterogeneous RNA sample comprising:
a) obtaining a fragmented RNA sample comprising:
i. RNA fragments of long RNA molecules; and
ii. unfragmented short RNA molecules; and
b) contacting said fragmented RNA sample with a first adaptor in the presence of a RtcB ligase, thereby producing a ligated
RNA sample comprising adaptor-ligated fragments of long RNA and unligated short RNAs.

US Pat. No. 9,267,630

FERRULE CHUCK WITH SURFACE MICRO-GRIP

Agilent Technologies, Inc...

1. A fitting for coupling a capillary to another component of a fluidic device, the fitting comprising
a ferrule configured for enclosing a front part of the capillary and for contributing to a fluidic sealing between the fitting
and the other component;

a ferrule chuck configured for enclosing a back part of the capillary;
a housing configured for accommodating at least a part of the ferrule chuck and for pushing the ferrule chuck against the
ferrule; and

a microstructure comprising multiple microprotrusions randomly distributed on at least a part of a surface of the ferrule
chuck for generating, wherein upon tightening the fitting at the other component, the microprotrusions are configured to exert
a gripping interaction from the surface of the ferrule chuck and penetrating into a surface of the capillary.

US Pat. No. 9,110,053

DRIED BLOOD SPOTTING PAPER DEVICE AND METHOD

Agilent Technologies, Inc...

1. A dried blood spotting device comprising:
a carrier card comprising a front face having a window therethrough, and a back face; and
punchable, untreated cellulose-free glass fiber paper disposed between the front face and the back face such that the glass
fiber paper is exposed in the window, wherein the front face and the back face are effective for supporting the glass fiber
paper during punching thereof, and the glass fiber paper is configured for uniform absorption of a blood droplet sample into
a homogeneous circular spot without a halo thereabout, and for enabling improved analysis for small molecules by liquid chromatography/mass
spectrometry of punched specimens of the sample absorbed glass fiber paper.

US Pat. No. 9,163,618

AUTOMATED CONVERSION BETWEEN SFC AND HPLC

Agilent Technologies, Inc...

1. An apparatus, comprising:
a source of CO2 supply; and
a fluidic switch configured to be selectively switched between: a first switch position which provides a first flow path configuration
through the switch for a liquid chromatographic (LC) system, and further provides a secondary flow path configuration; and
a second switch position which provides a second flow path configuration through the switch for a supercritical fluid chromatographic
system,

wherein the source of CO2 supply communicates with the secondary flow path configuration when the switch is in the first position,
and with the second flow path configuration when the switch is in the second position.

US Pat. No. 9,465,016

CHROMATOGRAPHIC SYSTEM AND METHOD OF ISOLATING COMPOUND IN SAMPLE USING CHROMATOGRAPHIC SYSTEM

Agilent Technologies, Inc...

1. A method of isolating a compound in a sample, comprising a plurality of compounds, by chromatography, the method comprising:
determining an analytical gradient of an analytical system;
performing an analytical run using the analytical gradient;
identifying a target compound in the sample using chromatographic results of the analytical run;
determining an analytical retention time of the target compound using the chromatographic results of the analytical run;
determining an elution point of the target compound using the analytical retention time, the analytical gradient and characteristics
of the analytical system;

determining automatically a focusing gradient of a preparative system, up-scaled from the analytical gradient, the focusing
gradient including a slope segment; and

performing a preparative run using the focusing gradient to separate the target compound from the sample,
wherein the slope segment of the focusing gradient includes a slope determining a concentration of solvent in a solvent mixture,
the concentration linearly increasing from a first offset below the elution point of the target compound to a second offset
above the elution point of the target compound.

US Pat. No. 9,291,605

VALVE FOR USE IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY HAVING A SPHERICAL SEAT WITH BEVELED OUTER FACES

Agilent Technologies, Inc...

1. A valve for use in high-performance chromatography, comprising;
a spherical seating structure having a sealing surface, and
a ball configured for constraining, when it abuts against the sealing surface of the spherical seating structure, a fluid
from flowing through the valve and for moving in an axial direction in order to allow the fluid to flow through the valve,

wherein the spherical seating structure has a beveled outer face separate and apart from the sealing surface, the beveled
outer face being inclined with respect to the axial direction, cooperating with a housing structure and being configured such
that a force acting on the valve along the axial direction generates a force acting on the ball, and

wherein the inclination of the beveled outer face is configured such that the force acting on the ball counteracts a force
exerted by the ball on the spherical seating structure and essentially compensates it.

US Pat. No. 9,165,751

SAMPLE ATOMIZATION WITH REDUCED CLOGGING FOR ANALYTICAL INSTRUMENTS

Agilent Technologies, Inc...

1. A method for producing an aerosol from a liquid sample, comprising:
providing a nebulizer comprising a first nebulizer tube terminating at a first nebulizer outlet, and a second nebulizer tube
coaxially surrounding the first nebulizer tube and terminating at a second nebulizer outlet;

flowing a carrier gas through the second nebulizer tube;
flowing the liquid sample through the first nebulizer tube such that the liquid sample becomes entrained in the carrier gas
and is broken into droplets to form the aerosol; and

flowing the aerosol out from the second nebulizer outlet, wherein:
the liquid sample comprises a high concentration of dissolved salt or dissolved solid material; and
the first nebulizer outlet comprises a sharp edge at which an inside diameter of the first nebulizer tube is substantially
the same as an outside diameter of the first nebulizer tube, such that the sharp edge prevents accumulation of precipitates
of salt or solid material at the first nebulizer outlet.

US Pat. No. 9,417,219

SAMPLE SEPARATION DEVICE WITH VALVE

Agilent Technologies, Inc...

1. A sample separation device for separating a sample, the sample separation device comprising:
a first fluid supply path for supplying a first fluid,
a second fluid supply path for supplying a second fluid,
a sample separation unit adapted for separating the sample,
a sensor configured for determining a value of a parameter related to the fluid, and
a valve configured for selectively coupling both of the first and second fluid supply paths to the sensor and to the sample
separation unit; or coupling one of the first and second fluid supply paths to the sensor, while blocking the fluid supply
path with the sensor coupled to the fluid supply path.

US Pat. No. 9,360,006

PRIMARY PISTON CORRECTION DURING TRANSFER

Agilent Technologies, Inc...

1. A method for controlling operation of a pump unit, the pump unit comprising a primary piston pump having a primary piston
and a secondary piston pump having a secondary piston, the primary piston pump being fluidically connected with the secondary
piston pump, and the pump unit operating periodically according to a pump cycle, the method comprising:
operating the pump unit according to a deliver-and-fill phase of the pump cycle during which the primary piston pump delivers
fluid to the secondary piston pump and to a fluidic system located downstream of the pump unit, while the secondary piston
performs an intake phase according to a predefined position-versus-time curve;

determining a fluid pressure of fluid dispensed by the pump unit; and
during a first time interval of the pump cycle, performing a closed loop control of a position of the primary piston in dependence
on the determined fluid pressure, while the secondary piston performs the intake phase according to the predefined position-versus-time
curve, wherein the first time interval corresponds to the deliver-and-fill phase.

US Pat. No. 9,335,265

SPECTROGRAPHIC SYSTEM UTILIZING A CHIRPED, PULSED OPTICAL SOURCE

Agilent Technologies, Inc...

1. An apparatus comprising:
a chirped light source that generates an optical signal;
a beam splitter that splits said optical signal into a reference optical signal and a sample optical signal;
a detector that mixes said sample optical signal and said reference optical signal, after said sample optical signal has traversed
an experimental sample and generates a signal having an AC component related to an attenuation and a dispersion introduced
by said experimental sample, wherein said reference optical signal traverses a reference optical path characterized by a reference
optical path length between said beam splitter and said detector, and wherein said sample optical signal traverses a sample
optical path characterized by a sample optical path length between said beam splitter and said detector, said sample optical
path length and said reference optical path length being chosen such that said reference optical signal and said sample optical
signal overlap in time but do not arrive at said detector at the same time.

US Pat. No. 9,255,245

SAMPLE PROBES AND METHODS FOR SAMPLING INTRACELLULAR MATERIAL

Agilent Technologies, Inc...

1. A sample probe, comprising:
a tip comprising a distal end extending along a first direction and configured for penetrating a cellular membrane, an opening
located at or proximal to the distal end, and a plurality of tip microchannels extending through the tip and communicating
with the opening; and

a body adjoining the tip and comprising a plurality of body microchannels, wherein the body extends along a second direction
perpendicular to the first direction, and at least one of the body microchannels communicates with at least one of the tip
microchannels,

wherein the plurality of tip microchannels or body microchannels comprises:
a first microchannel comprising a first section and a second section, the first section interconnecting the second section
with the opening and having a first characteristic dimension, and the second section having a second characteristic dimension
greater than the first characteristic dimension; and

a second microchannel communicating with the first microchannel at or near a junction between the first section and the second
section.

US Pat. No. 9,360,013

SCROLL PUMP HAVING AXIALLY COMPLIANT SPRING ELEMENT

Agilent Technologies, Inc...

1. A scroll pump, comprising:
a frame;
a stationary plate scroll fixed relative to the frame and having a stationary plate, and a stationary scroll blade projecting
axially from the stationary plate;

an orbiting plate scroll including an orbiting plate, and an orbiting scroll blade projecting axially from the orbiting plate,
and nested with the stationary scroll blade;

at least one tip seal, each said at least one tip seal interposed between an axial end of the scroll blade of a respective
one of the stationary and orbiting plate scrolls and the plate of the other of the stationary plate and orbiting plate scrolls;

an eccentric drive mechanism supported by the frame and operative to drive the orbiting plate scroll in an orbit about the
longitudinal axis,

the eccentric drive mechanism including a crankshaft having a main shaft and a crank, a counterbalance mounted to the crankshaft
so as to be slidable along the crankshaft in an axial direction parallel to the longitudinal axis of the pump, and bearings,

the orbiting plate scroll being carried by the crank and the main shaft of the crankshaft being supported by the frame via
said bearings, and one of the bearings being a flexure-locating bearing; and

a flexure axially interposed between said counterbalance and the flexure-locating bearing, the flexure having compliance in
the axial direction, wherein:

the flexure is an annular member having first and second opposite sides and radially innermost and outermost portions, and
the first side of the flexure has a first surface extending substantially perpendicular to a central axis of the annular member,
and a first projection that projects, at the radially outermost portion of the flexure, axially from the first surface in
a direction parallel to the central axis of the annular member.

US Pat. No. 9,335,309

FLUIDIC VALVE WITH SELECTIVELY SWITCHABLE STORAGE PATHS

Agilent Technologies, Inc...

1. A sample separation apparatus for separating a fluidic sample, the sample separation apparatus comprising:
a first separation unit for separating the fluidic sample;
a first fluid drive configured for generating a fluid flow for conducting the fluidic sample to be separated through the first
separation unit;

a second separation unit, arranged downstream of the first separation unit, for further separating the fluidic sample after
treatment by the first separation unit;

a second fluid drive configured for generating a fluid flow for conducting the fluidic sample or at least parts thereof, after
treatment by the first separation unit, through the second separation unit;

a fluidic valve having a first inlet fluidically coupled to one of the first fluid drive and the second fluid drive;
the fluidic valve having a second inlet fluidically coupled to the other of the first fluid drive and the second fluid drive;
the fluidic valve comprising at least two different sets of storage paths, wherein each set of storage paths comprises a first
storage path; and wherein the first storage path of a first set of said at least two sets of storage paths has a first volume
and the first storage path of a second set of said at least two sets of storage paths has a second volume different from the
first volume;

the fluidic valve being configured for selectively switching one set of said at least two sets of storage paths to the first
inlet and the second inlet.

US Pat. No. 9,260,747

ANALYSIS OF SINGLE NUCLEOTIDE POLYMORPHISMS USING END LABELING

Agilent Technologies, Inc...

1. A method for analyzing a sequence comprising a SNP (single nucleotide polymorphism) site, comprising:
a) contacting a first DNA sample with a first restriction enzyme to provide DNA fragments, wherein:
i) said first restriction enzyme cleaves said sequence at a cleavage site only if a first allele of a SNP is present at said
SNP site;

b) end-labeling said DNA fragments to produce an end-labeled sample;
c) hybridizing said end-labeled sample to an array comprising a probe sequence that:
i) hybridizes to an end-labeled polynucleotide of said end-labeled sample;
ii) hybridizes to a fragment produced if said first restriction enzyme cleaves said sequence at said cleavage site; and
iii) does not span said cleavage site;
d) comparing the amount of hybridization between the digested sample and the probe sequence to a reference signal, and
e) determining whether the first allele of the SNP is present in the DNA sample, wherein the relative hybridization of the
digested sample to the probe as compared to the reference signal indicates whether the first allele of said SNP is present
in the DNA sample.

US Pat. No. 9,079,178

APPARATUS AND METHODS FOR PIPETTING WITH INTERCHANGEABILITY AMONG DIFFERENT PIPETTE TIPS

Agilent Technologies, Inc...

1. A method for operating a pipettor, the method comprising:
selecting a first pipette tip from a plurality of pipette tips, the plurality of pipette tips comprising one or more of the
first pipette tips and one or more second pipette tips, wherein the first pipette tip comprises a first pipette tip proximal
end of a first interface geometry and the second pipette tip comprises a second pipette tip proximal end of a second interface
geometry different from the first interface geometry;

selecting a first adaptor from a plurality of hollow adaptors, the plurality of adaptors comprising one or more of the first
adaptors and one or more second adaptors, wherein the first adaptor comprises a first adaptor proximal end and a first adaptor
distal end, the first adaptor distal end is configured for interfacing with the first pipette tip proximal end, the second
adaptor comprises a second adaptor proximal end of a same interface geometry as the first adaptor proximal end and a second
adaptor distal end of a different interface geometry than the first adaptor distal end, and the second adaptor distal end
is configured for interfacing with the second pipette tip proximal end;

moving the pipettor to the selected first adaptor;
fluidly coupling the pipettor with the first adaptor;
moving the pipettor with the first adaptor to the selected first pipette tip; and
fluidly coupling the first adaptor with the first pipette tip.
US Pat. No. 9,286,438

SYSTEMS AND METHODS FOR PRODUCING CHEMICAL ARRAY LAYOUTS

AGILENT TECHNOLOGIES, INC...

1. A computer-based system for producing an array layout, said system comprising:
(A) an input manager for receiving array request information from a user;
(B) a processing module comprising a processor comprising:
(i) an array set developer configured to:
(a) identify a total number of features required to array display a selected portion of probe content of array request information;
and

(b) evaluate whether said identified total number of features exceeds a single array format feature number limit to determine
whether a single array format or an array set of two or more array formats is required to array display said total number
of features to provide an array set decision; and

(ii) an array layout developer having a memory comprising a plurality of rules relating to array layout design, wherein said
array layout developer is configured to develop an array set layout for an array set of two or more array formats based on
application of one or more of a plurality of rules to information that includes array request information received from a
user and said array set decision; and

(C) an output manager for providing a first version of said array set layout to said user.
US Pat. No. 9,085,762

COMPOSITIONS AND METHODS USING SPLIT POLYMERASES

Agilent Technologies, Inc...

1. A split Family B DNA polymerase wherein the split is at a non-natural split site within the fingers domain of the DNA polymerase
and the split polymerase exhibits increased incorporation of non-natural nucleotides or increased utilization of modified
primer templates relative to the non-split polymerase.

US Pat. No. 9,448,177

FLAME PHOTOMETRIC DETECTOR

Agilent Technologies, Inc...

1. An apparatus for receiving a gas chromatography column, the apparatus comprising:
a transfer line having a first end and a second end opposing the first end;
a heater block disposed around a first portion of the transfer line, the heater block comprising a heater configured to maintain
the first portion of the transfer line at a first temperature range;

an emission block configured to receive the second end of the transfer line; and
a body tube substantially surrounding a second portion of the transfer line such that a gap is provided between the body tube
and the second portion of the transfer line, the body tube mechanically and thermally coupled to the emission block, wherein
the emission block and the body tube are maintained at a second temperature range that is lower than the first temperature
range, and the second portion of the transfer line is maintained at the first temperature range.

US Pat. No. 9,418,826

ION OPTICAL SYSTEM FOR MASS SPECTROMETER

Agilent Technologies, Inc...

1. A mass spectrometer, comprising:
a plasma generation device for generating plasma for ionizing an introduced sample;
an interface device for drawing the plasma into vacuum;
an ion lens device for extracting and inducing ions as an ion beam from the plasma;
a mass analyzer for allowing a predetermined ion in the ion beam to pass along a first axis based on a mass-to-charge ratio;
an ion detector for detecting the ion;
at least one first ion deflection device disposed prior to the mass analyzer to conduct ion deflection; and
at least one second ion deflection device disposed between the mass analyzer and the ion detector and configured to generate
an electric field to deflect and induce the predetermined ion having passed the mass analyzer along the first axis so as to
be along a second axis to the ion detector,

wherein the at least one second ion deflection device comprises a first electrode positioned to deflect the ion beam toward
the second axis in response to application of a voltage to the first electrode, and at least one additional electrode positioned
on a side of the ion beam opposite to the first electrode.

US Pat. No. 9,341,516

SYSTEM FOR PERFORMING OPTICAL SPECTROSCOPY INCLUDING INTERFEROMETER

Agilent Technologies, Inc...

1. A system for performing optical spectroscopy measurements, comprising:
a light source configured to generate an input optical beam;
an interferometer configured to receive the input optical beam generated by the light source, the interferometer comprising:
a beam splitter configured to split the input optical beam into a first light beam and a second light beam;
a first light path configured to direct the first light beam through a sample to a first output port, the sample containing
an analyte of interest; and

a second light path configured to direct the second light beam to the first output port, at least one of the first light path
and the second light path being further configured to adjust a relative phase of a corresponding at least one of the first
light beam and the second light beam, respectively, so that the first light beam and the second light beam are out of phase
at the first output port, substantially canceling background light and outputting sample light corresponding to a portion
of the first light signal absorbed by the sample in the sample cell; and

a detection system configured to detect the sample light output at the first output port.

US Pat. No. 9,279,722

OPTICAL EMISSION SYSTEM INCLUDING DICHROIC BEAM COMBINER

Agilent Technologies, Inc...

1. A spectrometer system, comprising:
a light source configured to emit light from a sample, the emitted light comprising first light emitted in a first direction
and second light emitted in a second direction different from the first direction;

a light detection and measurement apparatus configured to perform emission spectrometry analysis on the sample by measuring
intensity and wavelength of each of the first light and the second light; and

a dichroic beam combiner configured to receive the first light via a first light path and the second light via a second light
path, to reflect a reflected portion of the first light into an entrance aperture of the light detection and measurement apparatus,
and to transmit a transmitted portion of the second light into the entrance aperture of the light detection and measurement
apparatus,

wherein the reflected portion of the first light reflected into the entrance aperture predominately has wavelengths in a first
range of wavelengths, and the transmitted portion of the second light transmitted into the entrance aperture predominately
has wavelengths in a second range of wavelengths different from the first range of wavelengths.

US Pat. No. 9,494,563

FITTING ELEMENT WITH BIO-COMPATIBLE SEALING

Agilent Technologies, Inc...

1. A fitting element configured for coupling a tubing to a fluidic device, the fluidic device comprising a receiving cavity
configured for receiving the fitting element, wherein the tubing comprises an inner contact surface comprising a biocompatible
material, the inner contact surface is configured to contact a fluid to be conducted by the tubing, and the receiving cavity
comprises a receiving contact surface comprising a bio-compatible material, the fitting element comprising:
a first sealing element comprising a bio-compatible material and being configured for sealing to the bio-compatible material
of the inner contact surface of the tubing; and

a second sealing element configured for sealing against a pressure ambient to a pressure of the fluid in the tubing,
wherein, upon coupling the tubing to the fluidic device via the fitting element, at least a portion of the receiving contact
surface, the first sealing element, and the second sealing element enclose an interspace, each surface of the interspace comprising
a bio-compatible material.

US Pat. No. 9,163,329

RNA LABELING METHOD

Agilent Technologies, Inc...

1. A method of sample analysis comprising:
a) obtaining a fragmented RNA sample comprising:
i. fragments of long RNA molecules, wherein the long RNA molecules are at least 200 nucleotides in length; and
ii. short RNA molecules of less than 200 nucleotides in length;
b) ligating an adaptor to an end of the RNA of said fragmented RNA sample to produce an adaptor-ligated sample that comprises
adaptor-ligated RNA molecules comprising:

i. adaptor-ligated short RNA comprising an adaptor portion and a short RNA portion; and
ii. adaptor-ligated long RNA fragments comprising an adaptor portion and a long RNA portion;
c) hybridizing said adaptor-ligated sample to a plurality of nucleic acid probes comprising:
i. a first nucleic acid probe that comprises a nucleotide sequence that is complementary to both said adaptor portion and
said short RNA portion of said adaptor-ligated short RNA; and

ii. a second probe that comprises a nucleotide sequence that is complementary to the long RNA portion of said adaptor-ligated
long RNA but not said adaptor portion of said long RNA; and

d) analyzing the adaptor-ligated RNA molecules that hybridize to said probes.

US Pat. No. 9,427,821

INTEGRATED MAGNETRON PLASMA TORCH, AND RELATED METHODS

Agilent Technologies, Inc...

1. A plasma source for generating microwave-induced plasma, comprising:
a microwave energy source comprising a main structure, the main structure comprising a cathode on a cathode axis, and an anode
spaced from the cathode by an interaction space, wherein the main structure further comprises a first side, a second side,
and a thickness from the first side to the second side along the cathode axis; and

a plasma torch positioned at the microwave energy source and comprising a torch outlet on the second side, wherein the plasma
torch establishes a gas flow path through the plasma torch from the first side to the second side, and the plasma torch is
positioned such that a gas flowing along the gas flow path in the plasma torch is irradiated by microwave energy generated
by the microwave energy source.

US Pat. No. 9,316,324

SHEAR VALVE WITH SILICON CARBIDE MEMBER

Agilent Technologies, Inc...

1. A shear valve for use in a high performance liquid chromatography system, the shear valve comprising:
a first shear valve member;
a second shear valve member; and
a housing for housing one of the first and the second shear valve members, wherein the housing is shrink-fit to the one of
the first and second shear valve members and is prestressed against the housed shear valve member to achieve sealing in pressurized
applications,

wherein at least one of the first and second shear valve members is configured to be moved with respect to the other, one
of the first and second shear valve members comprises a plurality of ports, and the other comprises at least one fluid path
for fluidly coupling respective ones of the ports in dependency on a relative movement position of the first and second shear
valve members with respect to each other,

wherein the first shear valve member is at least partially coated with or comprised of a ceramic material.
US Pat. No. 9,365,896

ADDITION OF AN ADAPTOR BY INVASIVE CLEAVAGE

Agilent Technologies, Inc...

1. A method comprising:
a) hybridizing genomic DNA to an adaptor, wherein the adaptor comprises:
a double stranded region;
a single stranded region comprising a 5? overhang; and
a nucleotide sequence for subsequent enzymatic processing;
wherein the 5? overhang hybridizes to the genomic DNA to produce a substrate for a flap endonuclease, and wherein said 5?
overhang consists of a sequence of N nucleotides and/or nucleotide analogs that base pair with any nucleotide, where N is
G, A, T or C;

b) cleaving said substrate using said flap endonuclease to produce a product comprising said adaptor and a flap endonuclease-generated
fragment of said genomic DNA that is hybridized to said 5? overhang and ligatable to the recessed end of said double stranded
region, wherein said cleaving occurs at a random position in said genomic DNA;

c) ligating the recessed end of said double stranded region to said fragment to produce an adaptor-ligated DNA;
d) intramolecularly ligating the ends of said adaptor-ligated DNA to produce a circular DNA molecule; and
e) enzymatically processing said circular DNA molecule using an oligonucleotide that hybridizes to said adaptor and an enzyme.

US Pat. No. 9,341,186

SCROLL VACUUM PUMP AND METHOD OF MAINTENANCE INCLUDING REPLACING A TIP SEAL OF A SCROLL VACUUM PUMP

Agilent Technologies, Inc...

1. A scroll pump comprising: an inlet portion having a pump inlet into which fluid is drawn by the pump, and an exhaust portion
including a pump outlet through which fluid is exhausted from the pump; a frame; a stationary plate scroll fixed to the frame
and including a stationary plate, and a stationary scroll blade projecting from the stationary plate; an orbiting plate scroll
including an orbiting plate, and an orbiting scroll blade projecting axially from the orbiting plate, the stationary scroll
blade having the form of a spiral including a plurality of successive wraps emanating from a central portion of the stationary
plate, the orbiting scroll blade having the form of a spiral including a plurality of successive wraps emanating from a central
portion of the orbiting plate, and the stationary and orbiting scroll blades being nested; a tip seal interposed between an
axial end of the scroll blade of one of the stationary and orbiting plate scrolls and the plate of the other of the stationary
plate and orbiting plate scrolls; an eccentric drive mechanism supported by the frame, and the orbiting plate scroll being
coupled to the eccentric drive mechanism so as to be driven by the eccentric drive mechanism in an orbit about a longitudinal
axis of the pump, wherein during the orbital motion of the orbiting plate scroll relative to the stationary plate scroll,
a series of pockets are simultaneously defined between the nested stationary and orbiting scroll blades, the series of pockets
constitute a compression stage of the pump, each of the pockets is selectively and sequentially placed in open communication
with the pump inlet and the pump outlet, and a compression process in which fluid trapped in the pocket is compressed occurs
between a point in time at which the pocket is in open communication with the pump inlet and a later point in time at which
the pocket is in open communication with the pump outlet; a ballast gas supply system operative to supply a stream of ballast
gas into the compression stage of the pump at a location near a downstream end of the compression stage with respect to the
direction of flow of fluid through the pump; and control means configured to calculate a monitored pressure value based on
both a first pressure of the fluid sensed in the pump at a location along the direction of flow when the ballast gas supply
system is disabled and is not supplying ballast gas into the compression stage, of the pump and a second pressure of the fluid
sensed at said location when the ballast gas supply system is enabled and is supplying ballast gas into the compression stage,
to compare the monitored pressure value with a reference pressure value, and to output a signal, indicative of a need to replace
the tip seal, when the monitored pressure value and the reference pressure value differ by at least a predetermined amount.

US Pat. No. 9,345,989

LOW NOISE BACK PRESSURE REGULATOR FOR SUPERCRITICAL FLUID CHROMATOGRAPHY

Agilent Technologies, Inc...

1. A device for a back pressure regulator, comprising:
a nozzle insert comprising;
an outlet flow channel that can receive an outlet flow tube and an inlet flow channel that can receive an inlet flow tube;
a seat, near an end of said insert, such that said seat can be in fluidic communication with said outlet flow channel; and
a nozzle body at least partially receiving said nozzle insert and said seat; and a diaphragm having a first surface positioned
adjacent to said seat wherein said nozzle body constrains a circumferential edge of said diaphragm.

US Pat. No. 9,353,749

AXIALLY COMPLIANT ORBITING PLATE SCROLL AND SCROLL PUMP COMPRISING THE SAME

Agilent Technologies, Inc...

1. An orbiting plate scroll, comprising:
an orbiting plate having a first side and a second side;
an orbiting scroll blade projecting in an axial direction from the first side of the orbiting plate; and
a flexure comprising a fixed end fixed to the orbiting plate at the second side of the orbiting plate, wherein the flexure
is configured and coupled to the orbiting plate such that the compliance of the flexure is substantially only in the axial
direction and at least a portion of the flexure is deflectable in the axial direction relative to the fixed end;

wherein the flexure comprises a cylindrical hub whose central longitudinal axis extends in the axial direction, and a flange
that extends from the hub and connects the hub to the orbiting plate, and

wherein the flange comprises a web that is spaced axially from the orbiting plate with the flexure relaxed such that a gap
exists between the web and the orbiting plate, and a union integral with the web and by which the flexure is integrated with
the orbiting plate at the fixed end.

US Pat. No. 9,587,268

FAST HYBRIDIZATION FOR NEXT GENERATION SEQUENCING TARGET ENRICHMENT

Agilent Technologies Inc....

1. A method for hybridizing a target nucleic acid to a bait nucleic acid, comprising
contacting a target nucleic acid with a bait nucleic acid in a composition and forming a hybridization mixture, said composition
comprising

(i) a salt of a divalent cation that has a concentration in a range from about 100 mM to about 600 mM,
(ii) a buffering agent, wherein said salt and said buffering agent are present in a molar ratio of about 2.5:1 to about 60:1,
and

(iii) a volume-excluding/thickening agent that has a concentration in a range from about 0.002% to about 0.1%, and
incubating the mixture for 8 hours or less,
wherein the volume-excluding/thickening agent is selected from the group consisting of hydroxypropyl methyl cellulose (HPMC),
hydroxyethyl methyl cellulose (HEMC), hydroxybutyl methyl cellulose, hydroxypropyl cellulose, methyl cellulose, and hydroxyl
methyl cellulose.

US Pat. No. 9,673,032

SAMPLE SPRAYER WITH ADJUSTABLE CONDUIT AND RELATED METHODS

Agilent Technologies Inc....

1. A sample sprayer, comprising:
a first conduit disposed along a longitudinal axis, the first conduit comprising a first inlet for receiving a flow of a liquid
sample, a first outlet for emitting the liquid sample, and a first conduit outer surface;

a second conduit surrounding the first conduit about the longitudinal axis, the second conduit comprising a second inlet for
receiving a flow of a gas and a second conduit inner surface spaced from the first conduit outer surface, wherein the first
conduit and the second conduit define an annular passage for conducting the gas;

a sprayer tip comprising a sprayer tip body and a fluid interaction region, wherein:
the sprayer tip body comprises an orifice disposed at an axial distance from the first outlet relative to the longitudinal
axis;

the fluid interaction region is disposed along the longitudinal axis between the first conduit and the orifice, and communicates
with the first outlet, the annular passage, and the orifice; and

the sprayer tip is configured to produce a sample spray by contact between the liquid sample and the gas in the fluid interaction
region and emit the sample spray from the orifice; and

an adjustable positioning device mechanically communicating with the first conduit and configured to translate the first conduit
along the longitudinal axis in response to adjustment of the positioning device, wherein an axial position of the first outlet
along the longitudinal axis is adjustable relative to the orifice.

US Pat. No. 9,459,239

INTAKE MONITORING FOR ACCURATE PROPORTIONING

Agilent Technologies, Inc...

1. A fluid supply system configured for metering two or more fluids in controlled proportions in accordance with a given metering
scheme and for supplying a resultant mixture, the fluid supply system comprising:
a plurality of solvent supply lines, each fluidically connected with a fluid source providing a respective fluid;
a pumping unit comprising an inlet, an outlet, and a reciprocating element configured for intaking fluid supplied at the inlet
and for supplying the pressurized fluid at the outlet, wherein the pumping unit is configured for taking in fluids from selected
solvent supply lines and for supplying a pressurized mixture of the fluids at the outlet;

a proportioning valve interposed between the solvent supply lines and the inlet, the proportioning valve configured for modulating
solvent composition by sequentially coupling selected ones of the solvent supply lines with the inlet;

a sensor configured for sensing process information in the fluid supply system relating to a fluid intake process of the pumping
unit;

an analysis entity configured for analyzing the sensed process information and for determining as a result of the analysis,
independently of a position of the reciprocating element within the pumping unit, the event of the beginning of a fluid intake
process, wherein the analysis entity is further configured for determining reciprocating element related information at the
event of the beginning of the fluid intake process; and

a control unit configured for switching the proportioning valve to sequentially couple selected ones of the solvent supply
lines to the inlet of the pumping unit at one or more switching points during a duty cycle of the reciprocating element coordinated
with the motion of the reciprocating element based on the given metering scheme and based on the reciprocating element related
information,

wherein the control unit is configured for coordinating operation of the pumping unit and the proportioning valve for at least
partially compensating for a discrepancy between an actual relationship between operation of the pumping unit and the proportioning
valve, as indicated by the reciprocating element related information determined by the analysis entity, and a target relationship
between operation of the pumping unit and the proportioning valve, as predefined for operating the fluid supply system.

US Pat. No. 9,461,443

OPTICAL SYSTEM HAVING REDUCED POINTING-ERROR NOISE

Agilent Technologies, Inc...

1. An apparatus comprising:
a variable angle light source characterized by a pivot point;
a variable response optical receiver having a receiving surface, said variable response optical receiver receiving light generated
by said variable angle light source on said receiving surface, said receiver generating a signal indicative of an intensity
of light that impinges on a receiving surface; and

a first optical system that images said pivot point to a fixed point relative to said receiving surface.

US Pat. No. 9,670,931

ROTARY VACUUM PUMP

Agilent Technologies Inc....

1. A rotary vacuum pump comprising:
a pump housing in which an inlet port and an outlet port are defined;
a plurality of pumping stages arranged inside the pump housing between the inlet port and the outlet port for pumping a gas
from the inlet port to the outlet port, each of the pumping stages comprising a stator stage integral with the pump housing
and a cooperating rotor disc integral with a rotating shaft centrally arranged in the housing, wherein the plurality of pumping
stages comprises:

a first set of pumping stages having rotor discs with a first outer diameter followed in the flow direction of the pumped
gas by a second set of pumping stages having rotor discs with a second outer diameter different from the first outer diameter;
and

an intermediate pumping stage interposed between the first set of pumping stages and the second set of pumping stages, the
intermediate pumping stage comprising:

a rotor disc of the intermediate pumping stage having an inner diameter, and an outer diameter between the first outer diameter
and the second outer diameter, and comprising an outer portion in a radial direction from the shaft that is axially tapered
from the inner diameter toward the outer diameter; and

a stator stage of the intermediate pumping stage comprising a stator ring cooperating with the rotor disc of the intermediate
pumping stage, and a spacer ring comprising a periphery and a center, wherein the spacer ring is axially tapered from the
periphery toward the center.

US Pat. No. 10,073,068

LIQUID CHROMATOGRAPHY COLUMNS WITH STRUCTURED WALLS

Agilent Technologies, Inc...

1. A liquid chromatography (LC) column, comprising:an inlet end;
an outlet end;
a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and comprising a main portion and a structured portion, wherein the structured portion comprises a helical structure, and the helical structure is continuous, or is partitioned into helical segments spaced from each other; and
a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion and other particles are in contact with the main portion.
US Pat. No. 9,523,085

THERMOSTABLE TYPE-A DNA POLYMERASE MUTANTS WITH INCREASED POLYMERIZATION RATE AND RESISTANCE TO INHIBITORS

Agilent Technologies Inc....

1. A mutant thermostable Type-A DNA polymerase consisting of or comprising:
a first mutation at residue 507 of wild-type Taq DNA polymerase, which has the sequence of SEQ ID NO:4, or at a residue corresponding
to residue 507 of wild-type Taq DNA polymerase in another thermostable Type-A DNA polymerase; and at least one additional
mutation at a residue selected from 59, 155, 245, 375, 508, 734, and 749 of wild-type Taq DNA polymerase, or at a corresponding
residue in another thermostable Type-A DNA polymerase,

wherein the combination of mutations provides a mutant polymerase that possesses a faster polymerization rate and a higher
resistance to polymerization activity inhibitors than the wild-type DNA polymerase from which it is derived.

US Pat. No. 9,343,281

METHODS AND APPARATUS FOR INCREASED ION THROUGHPUT IN TANDEM MASS SPECTROMETERS

Agilent Technologies, Inc...

1. A tandem mass spectrometry (MS) system, comprising:
a first mass analyzer configured for receiving a plurality of parent ions spanning a full parent mass range, wherein the full
parent mass range comprises N parent mass sub-ranges;

an ion fragmentation device;
a second mass analyzer;
an ion detector; and
a computing device configured for:
controlling the first mass analyzer, the ion fragmentation device, the second mass analyzer, and the ion detector according
to a modulation format comprising the following steps:

(i) in a first iteration, transmitting a first packet of the parent ions received by the first mass analyzer to a fragmentation
device, wherein the first packet spans the full parent mass range except for a first rejected sub-range, the first rejected
sub-range being one of the N parent mass sub-ranges;

(ii) fragmenting the parent ions of the first packet to produce a plurality of first daughter ions;
(iii) measuring the first daughter ions to acquire first daughter spectral data;
(iv) repeating steps (i) to (iii) for at least (N?1) additional iterations wherein, in each additional iteration, a new packet
of the parent ions is transmitted to the fragmentation device, the new packet spans the full parent mass range except for
a new rejected sub-range different from rejected sub-range of the first iteration and of any other previous iteration, the
new packet is fragmented to produce a plurality of new daughter ions, and the new daughter ions are measured to acquire new
daughter spectral data;

selecting one of the N parent mass sub-ranges; and
associating a group of measured daughter ions from the acquired daughter spectral data with the selected parent mass sub-range,
wherein the group corresponds to daughter ions produced from parent ions of the selected parent mass sub-range.

US Pat. No. 9,711,339

METHOD TO GENERATE DATA ACQUISITION METHOD OF MASS SPECTROMETRY

Agilent Technologies Inc....

19. In a plasma ion source mass spectrometry apparatus wherein a sample to be measured is supplied into plasma to ionize elements
in the sample, a beam of the generated ions is introduced into a mass spectrometer through a collision/reaction cell, and
ionized elements are separated and detected according to mass-to-charge ratio, a non-transitory computer program for automatically
creating a mass spectrometry method used for operating the plasma ion source mass spectrometry apparatus, the computer program
enabling a computer to execute:
a procedure for semi-quantitatively measuring at least all elements in the sample that affect the setting of measurement conditions,
using the plasma ion source mass spectrometry apparatus;

a procedure for determining a plasma condition based on the total concentration of the semi-quantitatively measured elements;
a procedure for estimating, for each of the semi-quantitatively measured elements, signal strengths of the element and interference
components in the sample and, based on the resultant estimates, estimating the concentration of the element; and

a procedure for, based on the estimated signal strengths of the elements and the interference components and the estimated
element concentration, determining at least one mass spectrometry method including at least one of:

(1) a plasma condition;
(2) a combination of internal standard elements used for correction at the time of quantitating an element to be measured;
(3) a tuning condition for the collision/reaction cell;
(4) a mass-to-charge ratio used in a mass spectrometer; and
(5) an integration time used in the mass spectrometer.

US Pat. No. 9,429,020

SCROLL PUMP HAVING AXIALLY COMPLIANT SPRING ELEMENT

Agilent Technologies, Inc...

1. A scroll pump, comprising:
a frame;
a stationary plate scroll fixed relative to the frame and having a stationary plate, and a scroll blade projecting axially
from the stationary plate in a direction parallel to a longitudinal axis of the pump;

an orbiting plate scroll including an orbiting plate, and an orbiting scroll blade projecting axially from the orbiting plate
in a direction parallel to the longitudinal axis, and nested with the stationary scroll blade,

at least one tip seal, each said at least one tip seal interposed between an axial end of the scroll blade of a respective
one of the stationary plate and orbiting plate scrolls and the plate of the other of the stationary plate and orbiting plate
scrolls;

an eccentric drive mechanism supported by the frame and operative to drive the orbiting plate scroll in an orbit about the
longitudinal axis, and including a counterbalance, a drive shaft, and bearings,

the drive shaft comprising a crankshaft having a main shaft and a crank,
the orbiting plate scroll being carried by the crank, and the main shaft of the crankshaft being supported by the frame via
said bearings, and

wherein the eccentric drive mechanism has an axially facing flexure-locating surface, one of the bearings is a flexure-locating
bearing, the crankshaft is supported such that it is movable axially relative to the flexure-locating bearing, and the flexure-locating
surface is a surface counterbalance; and

a flexure axially interposed between said axially facing flexure-locating surface and said flexure-locating bearing, the flexure
having compliance in an axial direction parallel to the longitudinal axis of the pump and configured to enable movement of
the flexure-locating surface to position the orbiting plate scroll in the axial direction.

US Pat. No. 9,423,384

METHOD TRANSFER BETWEEN FLUIDIC DEVICES CONSIDERING DEVIATIONS FROM IDEAL BEHAVIOR

Agilent Technologies, Inc...

1. An apparatus that converts an operation for controlling a first fluidic device to a corresponding operation for controlling
a second fluidic device, the apparatus comprising:
an input/output device configured to receive a first target operation for treating a first fluid by the first fluidic device,
wherein the first target operation defines a sequence of instructions for controlling the first fluidic device;

a database configured to store modeling parameters for the first and second fluidic devices;
and a processor programmed to:
receive the first target operation from the input/output device and receive the modeling parameters for the first and second
fluidic devices from the database, determine a real operation based on the first target operation and the modeling parameters
of the first fluidic device, wherein the real operation represents actual behavior of treating the first fluid when controlling
the first fluidic device with the first target operation, and

determine a second target operation, for treating a second fluid by the second fluidic device, based upon the real operation
and the modeling parameters of the second fluidic device, wherein the second target operation defines a sequence of instructions
for controlling the second fluidic device;

store the determined second target operation in the database; and
control the operation of the second fluidic device for treating the second fluid according to the determined second target
operation, or transfer the determined second target operation to a data processing unit for controlling the operation of the
second fluidic device for treating the second fluid according to the determined second target operation.

US Pat. No. 9,450,330

CONNECTOR ASSEMBLY FOR AN INDUCTIVELY COUPLED PLASMA SOURCE

Agilent Technologies, Inc...

1. A connector assembly configured to join an induction coil to a radio frequency generator to provide, together with a plasma
torch, an inductively coupled plasma source for a spectrometer, the connector assembly comprising:
a radial clamping member associated with the radio frequency generator, the radial clamping member comprising an internal
surface configured to receive an end of an induction coil; and

a sealing member, wherein the connector assembly is configured to provide a secure connection between the end of the induction
coil and the RF generator that is substantially electrically conductive and substantially liquid-tight without causing lasting
deformation of the radial clamping member and wherein the sealing member enables telescopic adjustment of the induction coil
position while maintaining the substantially liquid-tight secure connection between the end of the induction coil and the
RF generator; and wherein the tightening or fastening of the radial clamping member does not distort or deform the induction
coil in any way elastically or plastically.

US Pat. No. 9,435,339

VIBRATION/NOISE MANAGEMENT IN A SCROLL COMPRESSOR

Agilent Technologies, Inc...

1. A scroll pump comprising:
a pump head/motor assembly including a stationary plate scroll fixed in the pump, an orbiting plate scroll, a pump motor having
a rotary output, and a drive shaft coupling the pump motor to the orbiting plate scroll;

feet having bottom surfaces that constitute the bottom of the pump, whereby the bottom surfaces of the feet will rest against
a support surface when the pump is set on the support surface in its operating position;

a vibration isolation system to which the pump head/motor assembly is mounted;
a sound-muffling enclosure interposed between the vibration isolation system and the feet such that the sound-muffling enclosure
is also interposed between the pump head/motor assembly and the feet; and

a locking system by which the pump head/motor assembly can be locked independently of the vibration isolation system to the
sound-muffling enclosure, and by which the sound-muffling enclosure can be locked to the same support surface that the feet
are resting on independently of the feet and without the pump head/motor assembly being locked to the sound-muffling enclosure
such that the pump head/motor assembly is supported by the support surface only via the vibration isolation system,

wherein the pump can be selectively placed using the locking system in a first mode in which the feet are resting against
the support surface while the pump head/motor assembly is hard mounted to the sound-muffling enclosure such that it cannot
move relative to the sound-muffling enclosure, and

a second mode in which the feet are resting against the support surface while the pump head/motor assembly is not locked to
the sound-muffling enclosure and the sound-muffling enclosure is locked to the support surface independently of the feet,
whereby the sound-muffling enclosure can be hard mounted to the support surface such that it cannot move relative to the support
surface while the pump head/motor assembly is free mounted to the sound-muffling enclosure via the vibration isolation system
such that it can vibrate relative to the sound-muffling enclosure.

US Pat. No. 9,945,820

MIXER BYPASS SAMPLE INJECTION FOR LIQUID CHROMATOGRAPHY

Agilent Technologies, Inc...

1. A liquid chromatography (LC) system, comprising:a column;
a first solvent source;
a second solvent source;
a mixer communicating with the first solvent source via a first solvent line, with the second solvent source via a second solvent line, and with the column via a solvent mixture line;
a fluidic junction in the solvent mixture line; and
an injection valve comprising a sample loop separate from the solvent mixture line and communicating with the fluidic junction and an injection solvent source, wherein the injection valve is adjustable to a sample injection position that defines a flow path for an injection solvent through the sample loop and to the fluidic junction, such that the injection solvent carries a sample in the sample loop into the solvent mixture line downstream from the mixer.

US Pat. No. 9,921,191

GAS CHROMATOGRAPHIC (GC) UNIT, SCALABLE GC SYSTEMS USING SAME, AND RELATED METHODS

Agilent Technologies, Inc...

1. A gas chromatographic (GC) unit, comprising:
an inlet-side microfluidic device comprising an inlet body having a plurality of inlet-side channels and a plurality of inlet-side
ports, each inlet-side port communicating with at least one of the inlet-side channels, wherein a first inlet-side channel
extending inwardly from a first side of the inlet body is in communication with at least two other inlet-side channels extending
inwardly from different sides of the inlet body than the first side of the inlet body;

an outlet-side microfluidic device comprising an outlet body having a plurality of outlet-side channels and a plurality of
outlet-side ports, each outlet-side port communicating with at least one of the outlet-side channels, wherein a first outlet-side
channel extending inwardly from a first side of the outlet body is in communication with at least two other outlet-side channels
extending inwardly from different sides of the outlet body than the first side of the outlet body;

a column comprising a column inlet coupled to a first inlet-side port of the plurality of inlet-side ports, and a column outlet
coupled to a first outlet-side port of the plurality of outlet-side ports; and

a flow controller (FC) comprising at least one FC input port for receiving a flow of a carrier gas, a first FC output port
communicating with the at least one FC input port, and a second FC output port communicating with the at least one FC input
port, wherein the first FC output port communicates with an additional inlet-side port of the plurality of inlet-side ports
in communication with the column inlet of the column.

US Pat. No. 9,366,255

SCROLL VACUUM PUMP HAVING EXTERNAL AXIAL ADJUSTMENT MECHANISM

Agilent Technologies, Inc...

1. A scroll pump, comprising:
a frame;
a stationary plate scroll assembled and fixed relative to the frame, the stationary plate scroll comprising a stationary plate,
and a stationary scroll blade projecting axially from the stationary plate in a direction parallel to a longitudinal axis
of the scroll pump;

an orbiting plate scroll comprising an orbiting plate, and an orbiting scroll blade projecting axially from the orbiting plate
in a direction parallel to the longitudinal axis, and nested with the stationary scroll blade;

at least one solid plastic tip seal interposed between an axial end of at least one of the stationary scroll blade and the
orbiting scroll blade and the opposing one of the orbiting plate or the stationary plate;

an eccentric drive mechanism assembled to and supported by the frame, the orbiting plate scroll being assembled to and supported
by the eccentric drive mechanism, and the eccentric drive mechanism being operative to drive the orbiting plate scroll in
an orbit about the longitudinal axis,

wherein the frame, the stationary plate scroll, the orbiting plate scroll and the eccentric drive mechanism constitute respective
parts of a pump head of the scroll pump, which are assembled to one another; and

a threaded fastener that adjusts the distance, in the longitudinal direction, between the stationary plate and the orbiting
plate while said parts of the pump head remain assembled to one another.

US Pat. No. 9,945,762

APPARATUS AND METHOD FOR INTRODUCING SAMPLE INTO A SEPARATION UNIT OF A CHROMATOGRAPHY SYSTEM WITHOUT DISRUPTING A MOBILE PHASE

Agilent Technologies, Inc...

1. A system for introducing a sample into a mobile phase directed to a separation unit of a chromatography system, the system comprising:a mobile phase pumping system configured to meter a combined solvent as the mobile phase, the mobile phase comprising a strong solvent and a weak solvent, and pressurize the mobile phase, the mobile phase pumping system further configured to direct a flow of the pressurized mobile phase to the separation unit;
a booster pump configured to pressurize the weak solvent and deliver a first portion of a flow of the pressurized weak solvent to the mobile phase pumping system and a second portion of the flow of the pressurized weak solvent to a sample loop; and
a valve assembly disposed upstream from the separation unit and configured to pressurize the sample loop loaded with the sample using the second portion of the flow of the pressurized weak solvent, the second portion supplied to the valve assembly being pressurized by the booster pump, and subsequently to switch the pressurized sample loop into the pressurized mobile phase, thereby introducing the pressurized sample into the pressurized mobile phase prior to introduction of the pressurized sample into the separation unit;
wherein the mobile phase from the mobile phase pumping system remains pressurized while the sample loop is being pressurized by the booster pump.

US Pat. No. 9,435,773

SAMPLE INJECTOR WITH METERING DEVICE BALANCING PRESSURE DIFFERENCES IN AN INTERMEDIATE VALVE STATE

Agilent Technologies, Inc...

1. A sample injector for use in a fluid separation system for separating compounds of a fluidic sample in a mobile phase,
the sample injector comprising:
a switchable valve;
a sample loop in fluid communication with the valve and configured for receiving the fluidic sample;
a metering device positioned so as to form a part of the sample loop and configured for introducing a metered amount of the
fluidic sample into a portion of the sample loop; and

a control unit configured for controlling the valve to switch among a first position, a second position and an intermediate
position in order to transfer the sample loop between a low pressure corresponding to the first position of the valve and
a high pressure corresponding to the second position of the valve,

the control unit being further configured for controlling the metering device to vary the pressure in the sample loop, while
the valve is in the intermediate position, between the low pressure for loading the fluidic sample and the high pressure for
driving the mobile phase,

wherein the metering device and the sample loop are in fluid communication in each of the first position and the second position
of the valve.

US Pat. No. 9,416,407

METHOD FOR GENOME ANALYSIS

Agilent Technologies, Inc...

1. A method of analyzing a test genome, comprising:
a) contacting under primer extension conditions a genomic sample comprising said test genome with a set of at least 10 sequence-specific
primers that are complementary to sites in only a top strand strand of a reference chromosomal region from the same species
as said test genome, to produce primer extension products; and

b) hybridizing said primer extension products to an array of probes comprising:
i. first probes that are complementary to the top strand of said reference chromosomal region and
ii. second probes that are complementary to the bottom strand of said reference chromosomal region;
to obtain hybridization results; and
c) analyzing said hybridization results to identify a chromosomal inversion or translocation in said test genome.

US Pat. No. 9,373,488

THIN FIELD TERMINATOR FOR LINEAR QUADRUPOLE ION GUIDES, AND RELATED SYSTEMS AND METHODS

Agilent Technologies, Inc...

1. An ion guide assembly, comprising:
a quadrupole ion guide comprising an entrance end, an exit end, and four guide electrodes elongated along a guide axis from
the entrance end to the exit end and positioned at a radial distance from the guide axis;

a quadrupole lens comprising four plates spaced from each other around the guide axis and positioned at an axial distance
from the entrance end or the exit end, wherein each plate is axially aligned with a respective one of the guide electrodes;
and

a direct current (DC) voltage source configured for applying DC potentials to the guide electrodes and the plates effective
for generating a DC quadrupolar field in the ion guide volume and terminating the DC quadrupolar field at the plates.

US Pat. No. 9,297,036

NUCLEIC ACID PROBES FOR ANALYSIS OF SMALL RNAS AND OTHER POLYNUCLEOTIDES

AGILENT TECHNOLOGIES, INC...

1. A nucleic acid duplex, comprising a polynucleotide and a probe that are base-paired with each other,
a) wherein said polynucleotide-comprises a labeled nucleotide added to the 3?end of said polynucleotide; and
b) wherein said probe comprises:
i) a region that is base-paired with said polynucleotide to form a duplex; and
ii) a Tm enhancement domain comprising: a) a nucleotide clamp that is base-paired with said labeled nucleotide and b) a hairpin region,
wherein said Tm enhancement domain increases stability of said duplex via coaxial stacking, and wherein said probe is bound to a surface of
a solid support by its 3? terminus.

US Pat. No. 9,589,775

PLASMA CLEANING FOR MASS SPECTROMETERS

Agilent Technologies, Inc...

1. A mass spectrometry (MS) system, comprising:
an ion source configured for producing analyte ions;
a chamber;
an internal surface located in the chamber;
a plasma source configured for generating or flowing plasma in the chamber effective for cleaning the internal surface, the
plasma source comprising a microwave power source, a plasma outlet, and a housing communicating with the chamber via the plasma
outlet, wherein the plasma source is configured for applying a microwave power sufficient to generate plasma both in the housing
and in the chamber; and

a controller configured for switching between operating the MS system in an analytical mode and a cleaning mode, wherein:
during the analytical mode, the controller is configured for producing analytical data from the ion measurement signals; and
during the cleaning mode the controller is configured for operating the plasma source to generate or flow the plasma in the
chamber.

US Pat. No. 9,513,265

PRESSURIZABLE INJECTION SEAT

Agilent Technologies, Inc...

1. An injection device, comprising:
a housing defining a space;
a seat located in said space, the seat being configured for receiving a flow path element at a flow path connection site in
the space, wherein the flow path element is configured for injecting a pressurized fluid through the seat; and

a pressure source for pressurizing a medium surrounding the flow path connection site in said space, thereby reducing the
pressure difference between the pressurized fluid in said flow path element and said medium.

US Pat. No. 10,145,823

GAS CHROMATOGRAPHY (GC) COLUMN HEATER CONTROL USING MULTIPLE TEMPERATURE SENSORS

AGILENT TECHNOLOGIES, INC...

1. A gas chromatography column temperature control apparatus, comprising:a column heating apparatus;
a first temperature sensor located within or directly adjacent to the column heating apparatus;
a second temperature sensor disposed above the column heating apparatus; and
a gas chromatography column disposed between the first temperature sensor and the second temperature sensor, wherein a temperature of the gas chromatography column is altered based on temperature data from the first and second temperature sensors.

US Pat. No. 9,605,674

VACUUM SCROLL PUMP HAVING PRESSURE-BALANCED ORBITING PLATE SCROLL

Agilent Technologies, Inc...

1. A vacuum scroll pump, comprising:
an inlet portion having a pump inlet, and an exhaust portion having a pump outlet;
a frame;
a stationary plate scroll fixed to the frame and comprising a stationary plate comprising a central portion, a back side,
a front side, and a stationary scroll blade projecting from the front side, wherein the stationary scroll blade has the form
of a spiral comprising a plurality of successive wraps emanating from the central portion of the stationary plate;

an orbiting plate scroll comprising an orbiting plate comprising a central portion, a back side, a front side that faces the
front side of the stationary plate, and an orbiting scroll blade projecting axially from the front side of the orbiting plate
toward the front side of the stationary plate, wherein the orbiting scroll blade has the form of a spiral comprising a plurality
of successive wraps emanating from the central portion of the orbiting plate,

and wherein the stationary scroll blade and the orbiting scroll blade are nested such that pockets are delimited by and between
the stationary scroll blade and the orbiting scroll blade, the pockets being disposed in series between the pump inlet and
the pump outlet and collectively constituting a compression stage of the vacuum scroll pump;

an eccentric drive mechanism supported by the frame and operatively connected to the orbiting plate scroll so as to cause
the orbiting plate scroll to orbit about a longitudinal axis of the vacuum scroll pump, and the orbiting plate scroll being
supported by the eccentric drive mechanism so as to be rotatable about a second axis parallel to the longitudinal axis; and

a tubular bellows comprising a first end connected to the orbiting plate at the back side thereof and a second end connected
to the frame,

wherein an axial counterbalancing chamber is defined within the frame at least in part by the tubular bellows, and is aligned
in the direction of the longitudinal axis with part of the back side of the orbiting plate, and

the orbiting plate has a gas bypass passage connecting the counterbalancing chamber and an intermediate one of said series
of pockets that constitute the compression stage; and

axial gas force control means for selectively placing said intermediate one of the pockets in open communication with the
counterbalancing chamber and for closing off communication between said intermediate one of the pockets and the counterbalancing
chamber, via the gas bypass passage.

US Pat. No. 9,451,971

INTRAMEDULLARY FIXATION ASSEMBLY AND DEVICES AND METHODS FOR INSTALLING THE SAME

Agilent Technologies, Inc...

1. An intramedullary fixation member for use in repairing a distal radius bone fracture, the distal radius having an articular
surface, the intramedullary fixation member comprising:
an elongate fixation member sized for insertion into a medullary canal of the radius, the elongate fixation member having
a first end and a second end, the second end adapted for placement within the medullary canal in spaced relation to the articular
surface of the distal radius, a concave side and a convex side extending between the first and second ends, the concave and
concave sides each defining a radius of curvature, the elongate fixation member defining a first fastener opening proximal
to the first end, the first fastener opening extending transversely through the elongate fixation member along a first fastener
axis and configured to receive a first fastener therethrough such that the first fastener such that a first fastener received
therethrough extends laterally outward of the fixation member, the first end of the fixation member defining additional fastener
openings disposed between the first fastener opening and the second end, the additional fastener openings extending transversely
through the fixation member along respective additional fastener axes, the additional fastener axes extending at different
angles with respect to the first fastener axis and to each other, each of the additional fastener openings being configured
to receive an additional fastener therethrough, wherein a first u-shaped slot is defined on a first side of the elongate fixation
member and a second u-shaped slot is defined on a second side of the elongate fixation member, wherein the u-shaped slots
are sized and configured to couple a driving handle to the elongate fixation member, and wherein a tail section extends from
the second end of the fixation member, the tail section having two spaced arms having opposing outer surfaces and configured
to be urged laterally outward in respective first and second opposing directions and into contact with a cortical wall defining
the medullary canal of the radius, wherein the tail section has a reduced maximum thickness measured across the opposing outer
surface of a first one of the spaced arms to the opposing outer surface of a second one of the spaced arms in a first position
as compared to a thickness of the first end of the fixation member such that each arm is configured to move from the first
position wherein the maximum thickness of the spaced arms measured across the opposing outer surfaces is smaller than the
thickness of the first end to a second position laterally outward from the first position, and wherein the tail section is
substantially straight; wherein the intramedullary fixation member further comprises a guide assembly attached to the elongate
fixation member by a threaded fastener extending through the first fastener opening.

US Pat. No. 9,377,468

ION MOBILITY MASS SPECTROMETRY TAGS FOR QUANTITATIVE APPLICATIONS AND METHODS THEREOF

Agilent Technologies, Inc...

15. A method for analyzing peptides, comprising:
reacting a sample comprising peptides with one or more compounds having the formula (I) thereby forming one or more compounds
of the formula (II):

A-Q-X-L or A-X-Q-L  (I)
A-Q-X-L-T or A-X-Q-L-T  (II)
wherein
A is a group comprising a repeatable monomer structural unit;
Q is a crown ether;
X is a cleavable group comprising a cleavable moiety that is cleavable via collision-induced dissociation (CID), electron-capture
dissociation (ECD), electron-transfer dissociation (ETD), higher-energy collisional dissociation (HCD, or pulsed-Q dissociation
(PQD;

L is a linker comprising repeatable monomer structural unit; and
T is a peptide comprising terminal guanidinium moiety, wherein the guanidinium moiety is capable of forming intra-molecular
complex with Q;

separating the formed compounds of formula (II) by ion mobility; and
causing cleavage of the cleavable group and dissociation of the compounds of formula (II) thereby forming fragments.
US Pat. No. 10,093,962

FAST HYBRIDIZATION FOR NEXT GENERATION SEQUENCING TARGET ENRICHMENT

Agilent Technologies, Inc...

1. A composition for nucleic acid hybridization, comprisinga salt of a divalent cation that has a concentration in the range from about 100 mM to about 600 mM,
a buffering agent, wherein said salt and said buffering agent are present in a molar ratio of about 2.5:1 to about 60:1, and
a volume-excluding/thickening agent that (i) is selected from the group consisting of hydroxypropyl methyl cellulose (HPMC), hydroxyethyl methyl cellulose (HEMC), hydroxybutyl methyl cellulose, hydroxypropyl cellulose, methyl cellulose, and hydroxyl methyl cellulose and (ii) has a concentration in the range from about 0.002% to about 0.1% w/w.

US Pat. No. 9,212,747

SEALING CONFIGURATION WITH METAL-COATED STRUCTURE

Agilent Technologies, Inc...

1. A sealing configuration configured for sealingly coupling a first fluid flow path of a first device to a second fluid flow
path of a second device, the sealing configuration comprising:
a support body comprising an upper surface, a lower surface, and a through hole between the upper surface and the lower surface,
wherein the through hole couples the first fluid flow path to the second fluid flow path when the sealing configuration is
arranged between the first device and the second device, and at least one of the upper surface and the lower surface comprises
a structure configured for increasing a surface pressure when coupling the first device to the second device, the structure
comprising one or more indentations; and

a metal coating configured for increasing sealing, the metal coating disposed between one or more indentations on at least
one raised contact surface of at least one of the upper surface and the lower surface on a same side of the support body as
the structure, wherein the metal coating comprises a material softer than a material of the support body.

US Pat. No. 10,455,683

ION THROUGHPUT PUMP AND METHOD

Agilent Technologies, Inc...

1. An ion throughput pump (ITP), comprising:a pump inlet configured to communicate with a vacuum chamber;
an ionization source downstream from the pump inlet and the vacuum chamber, the ionization source configured for ionizing gas species received from the vacuum chamber, wherein the pump inlet defines a path for the gas species to enter the ionization source from the vacuum chamber, and wherein the ionization source comprises a magnet assembly;
a pump outlet;
ion optics arranged generally along a longitudinal axis of the ITP, and configured for accelerating ions produced by the ionization source toward the pump outlet; and
a roughing pump stage spaced from the pump inlet along the longitudinal axis, the roughing pump stage configured for receiving the ions from the ionization source, producing neutral species from the ions, and pumping the neutral species through the pump outlet, wherein:
the ionization source defines an ionization region between the pump inlet and the roughing pump stage;
the ion optics are configured for generating an electric field through the ionization region oriented along the longitudinal axis; and
the magnet assembly is configured to generate a magnetic field that in the ionization region is predominantly oriented in a radial direction orthogonal to the longitudinal axis, such that the ionization source generates a Hall-effect plasma in the ionization region.

US Pat. No. 10,072,260

TARGET ENRICHMENT OF RANDOMLY SHEARED GENOMIC DNA FRAGMENTS

AGILENT TECHNOLOGIES, INC...

1. A method comprising:(a) hybridizing randomly sheared genomic DNA to a halo probe to produce a first circular complex, wherein said halo probe comprises:
(i) a first oligonucleotide comprising flanking sequences that hybridize to different regions in a fragment of the randomly sheared genomic DNA and a central sequence: and
(ii) one or more second oligonucleotides that are complementary to the central sequence of the first oligonucleotide;
(b) enzymatically digesting the overhanging ends of the genomic fragment in the first circular complex to provide a second circular complex in which 5? and 3? ends of the one or more second oligonucleotide are ligatably adjacent to the 3? and 5? ends of the digested genomic fragment; and
(c) ligating the ends of the digested genomic fragment of (b) to the ends of the one or more second oligonucleotide to produce a circular DNA molecule.

US Pat. No. 9,726,875

SYNTHESIZING LIGHT FIELDS IN MICROSCOPY

Agilent Technologies, Inc...

1. A method for synthesizing a light field representation of a sample, the method comprising:
acquiring bright field image data for a plurality of pixels of the sample;
acquiring phase image data for the plurality of pixels;
calculating mean ray pointing direction data, (?x, ?y), for the plurality of pixels based on the acquired phase image data, based on the following relation: [?x, ?y]??/2???(x, y), wherein ?(x, y) is the phase image data, ? is the wavelength of light utilized to acquire the phase image
data, and ??(x, y) is the gradient of the phase image data; and

calculating a light field based on the calculated mean ray pointing direction data and the acquired bright field image data.

US Pat. No. 9,536,723

THIN FIELD TERMINATOR FOR LINEAR QUADRUPOLE ION GUIDES, AND RELATED SYSTEMS AND METHODS

Agilent Technologies, Inc...

1. An ion guide assembly, comprising:
a quadrupole ion guide comprising an entrance end, an exit end, and four guide electrodes elongated along a guide axis from
the entrance end to the exit end and positioned at a radial distance from the guide axis;

a quadrupole lens comprising four plates spaced from each other around the guide axis and positioned at an axial distance
from the entrance end or the exit end, wherein each plate is axially aligned with a respective one of the guide electrodes;
and

a direct current (DC) voltage source configured for applying DC potentials to the guide electrodes and the plates effective
for generating a DC quadrupolar field in the ion guide volume and terminating the DC quadrupolar field at the plates.

US Pat. No. 10,072,261

DOUBLE COUPLING METHOD FOR OLIGONUCLEOTIDE SYNTHESIS

Agilent Technologies, Inc...

1. A method for double coupling a nucleoside phosphoramidite during synthesis of an oligonucleotide, the method comprising:(a) contacting a free hydroxyl group of a terminal nucleoside residue attached to a solid phase support with a first sample of a protected nucleoside phosphoramidite to couple the protected nucleoside to the terminal nucleoside residue via an internucleoside P(III) linkage;
(b) after step (a), exposing the contacted nucleoside residue to an oxidizing agent to oxidize the linkage and produce a first coupled and oxidized product;
(c) after step (b), contacting the first coupled and oxidized product with a second sample of the protected nucleoside phosphoramidite to couple the protected nucleoside to residual free hydroxyl groups of the terminal nucleoside residue via an internucleoside P(III) linkage; and
(d) after step (c), adding an oxidizing agent to oxidize the linkage and produce a protected terminal nucleoside residue.

US Pat. No. 9,831,078

ION SOURCE FOR MASS SPECTROMETERS

Agilent Technologies, Inc...

1. An ion source, comprising:
an ion funnel comprising a first opening at a first end, a second opening at a second end disposed at a distance from the
first opening along an axis, and a plurality of electrodes between the first end and the second end, wherein the first opening
is configured to receive neutral analyte molecules, and the electrodes are configured to generate a time-dependent electric
field for confining ions in a radial direction orthogonal to the axis and to generate a static electric field oriented along
the axis for guiding ions toward the second opening; and

an ionization device disposed in the ion funnel and configured to emit electromagnetic radiation or electrons to ionize the
neutral analyte molecules in the ion funnel.

US Pat. No. 9,803,782

FITTING ELEMENT WITH HYDRAULIC GRIP FORCE ELEMENT

Agilent Technologies, Inc...

1. A fitting element, in particular for an HPLC application, configured for providing a fluidic coupling of a tubing to a
fluidic device, the fitting element comprising:
a gripping piece configured to exert, upon coupling of the tubing to the fluidic device, a grip force (G) between the fitting
element and the tubing, wherein the gripping piece comprises a hydraulic element configured to transform an axial force (S)
into a hydraulic pressure (P) within the hydraulic element;

a housing for housing the hydraulic element and for retaining the hydraulic element in a certain space, wherein the gripping
piece at least partially defines the housing;

a rigid piston movable in an axial direction of an elongation of the tubing for contacting the hydraulic element and exerting
the axial force (S) on the hydraulic element; and

a first joint element movable in the axial direction for contacting the piston and pushing the piston in the axial direction
and into contact with the hydraulic element, wherein:

the first joint element is configured for movably engaging a second joint element of the fluidic device by a screw connection,
such that movably engaging the second joint element moves the first joint element in the axial direction; and

the hydraulic pressure (P) in the hydraulic element causes the grip force (G).

US Pat. No. 9,803,627

SERIAL TYPE PUMP COMPRISING A HEAT EXCHANGER

Agilent Technologies, Inc...

1. A pump unit comprising:
a primary piston pump,
a secondary piston pump,
a flow path fluidically connecting in series the primary piston pump and the secondary piston pump, wherein:
the pump unit is configured for executing a duty cycle comprising a delivery-and-fill phase, in which the primary piston pump
supplies a flow of liquid to the secondary piston pump, and during the delivery-and-fill phase, the flow of liquid supplied
by the primary piston pump is partly used for filling up the secondary piston pump and partly used for maintaining continuous
flow of liquid dispensed at an outlet of the secondary piston pump,

the flow path comprises a heat exchanger, wherein liquid supplied by the primary piston pump passes through the heat exchanger
before being supplied to the secondary piston pump, and

the heat exchanger is configured for reducing a temperature difference between a temperature of liquid supplied to the heat
exchanger and a temperature of the secondary piston pump influenced by flow rates of liquid supplied by the primary piston
pump, and enforced by the heat exchanger, during the delivery-and-fill phase, in that the heat exchanger is kept at a temperature
of the secondary piston pump, so that after having passed the heat exchanger, liquid supplied to the secondary piston pump
during the delivery-and-fill phase has substantially the same temperature as the temperature of the secondary piston pump,

and further comprising one or more of the following:
a thermostatic heating element configured to keep both the heat exchanger and the secondary piston pump at a predefined temperature;
the heat exchanger comprises a heat reservoir and one or more capillaries in thermal contact with the heat reservoir;
a control unit configured to superpose at least one corrective movement to at least one of the primary piston pump and secondary
piston pump so as to compensate for volumetric effects that are due to temperature variations of the liquid;

a common heat reservoir thermally coupling the heat exchanger and the secondary piston pump;
the pump unit being configured to execute the duty cycle such that the delivery-and-fill phase extends over less than 10%
of the duty cycle; or

the heat exchanger comprising a pattern of heat exchange elements configured to intensify the thermal contact of liquid to
the heat exchanger.

US Pat. No. 9,771,575

METHODS FOR ON-ARRAY FRAGMENTATION AND BARCODING OF DNA SAMPLES

Agilent Technologies, Inc...

1. A method of tagging DNA in a plurality of DNA samples, the method comprising:
(i) providing a first planar solid substrate comprising a plurality of spatially-addressable features, wherein each feature:
(a) is in a separate aqueous droplet; and
(b) comprises a transposase complex, wherein the transposase complex is bound to the surface of the first planar solid substrate
and comprises a first oligonucleotide domain comprising a unique barcode sequence; and

(ii) combining the plurality of features with one or more DNA samples that are on a second planar solid substrate, wherein
the combining is done under conditions that permit transposase-mediated tagging of DNA,

to produce a plurality of tagged DNA samples.

US Pat. No. 9,625,695

MAGNIFYING ASSEMBLY FOR AN INFRARED MICROSCOPE

Agilent Technologies, Inc...

1. An infrared microscope comprising:
a sample stage configured to support a sample;
an objective configured to focus radiation emanated from the sample to an intermediate image plane between the objective and
an infrared detector; and

a magnifying assembly comprising a first set of reflective elements provided in a fixed position and a second set of reflective
elements, the second set of reflective elements configured to move between an inoperative position in which radiation is incident
on the first set of reflective elements and an operative position in which the radiation is incident on the second set of
reflective elements and the second set of reflective elements effectively blocks the first set of reflective elements, wherein
magnification provided by the magnifying assembly is varied.

US Pat. No. 9,590,583

ALTERNATING CURRENT (AC) COUPLER FOR WIDEBAND AC SIGNALS AND RELATED METHODS

Agilent Technologies, Inc...

1. An AC coupler for transmitting high-frequency components of a wideband signal, the AC coupler comprising:
an input end for receiving the wideband signal;
an output end for transmitting an output signal;
a signal conductor in electrical communication between the input end and the output end, the signal conductor comprising a
conductive element and a signal conductor capacitor configured to block direct current (DC) components of the wideband signal
on the order of kilovolts or greater while transmitting high-frequency alternating current (AC) components of the wideband
signal; and

a shielding structure defining a signal return path between the input end and the output end, the shielding structure being
configured for conducting at least the AC components of the wideband signal in the signal return path, wherein:

the signal conductor has a signal conductor width;
the shielding structure has a width substantially greater than the signal conductor width to confine electric fields and currents
in the shielding structure substantially to a region proximate to the signal conductor; and

the signal conductor and the shielding structure are arranged as a transmission line.

US Pat. No. 10,480,979

FLOW METERS, FLOW METER CARTRIDGES, AND RELATED METHODS

Agilent Technologies, Inc...

10. A flow meter, comprising:a mainframe comprising a receptacle; and
a cartridge comprising a cartridge housing enclosing a cartridge interior, and configured for removable installation in the mainframe, a fluid inlet configured for conducting fluid from outside the cartridge housing to the cartridge interior, a chamber disposed in the cartridge interior, a chamber inlet communicating with the chamber, a flow sensor disposed in the cartridge interior and configured for measuring fluid flow rate into the chamber, and a fluid outlet, wherein:
the flow sensor comprises a displacement transducer disposed in the chamber;
the cartridge defines a flow path through the fluid inlet, through the chamber inlet, to the chamber, and toward the fluid outlet; and
the cartridge is movable through the receptacle between an uninstalled position at which the cartridge is unengaged with the mainframe, and an installed position at which the cartridge is engaged with the mainframe.

US Pat. No. 9,890,190

PREPARATION OF LONG SYNTHETIC OLIGONUCLEOTIDES BY SQUARATE CONJUGATION CHEMISTRY

Agilent Technologies, Inc...

1. A method of conjugating oligonucleotides, comprising:
activating a terminal of first oligonucleotide using a squarate reagent to produce an activated first oligonucleotide; and
binding the first oligonucleotide and a second oligonucleotide to a splint oligonucleotide;
to conjugate the activated first oligonucleotide with a terminal of the second oligonucleotide via a squaramide linkage to
produce a squaramide-linked oligonucleotide.

US Pat. No. 9,804,609

MASS FLOW CONTROLLERS AND METHODS FOR AUTO-ZEROING FLOW SENSOR WITHOUT SHUTTING OFF A MASS FLOW CONTROLLER

Agilent Technologies, Inc...

1. A mass flow controller, comprising:
a proportional valve having a first port connected to a first external line;
a mass flow sensor;
a first flow line connecting from a second port of the proportional valve through the mass flow sensor to a second external
line, wherein one of the first external line and the second external line is an entry line for connection to a fluid source
and the other of the first external line and the second external line is an exit line;

a second flow line joining the first flow line at a first junction located upstream of the mass flow sensor and at a second
junction located downstream of the mass flow sensor;

a switching valve placed at the first junction or the second junction to join the first flow line and the second flow line
such that the switching valve can regulate a flow of a fluid through the first flow line or the second flow line, wherein
the switching valve is switchable between a first state at which the switching valve allows the fluid to flow through the
first flow line, and a second state at which the switching valve allows the fluid to flow through the second flow line while
producing a zero-flow condition at the mass flow sensor; and

a feedback control comprising control electronics and a feedback control loop connecting the control electronics with the
proportional valve and the mass flow sensor, said feedback control configured to regulate the proportional valve based on
signals measured by the mass flow sensor, wherein the feedback control is configured to send a control signal to the proportional
valve effective for keeping a flow rate of fluid exiting the exit line substantially constant while the switching valve is
in the second state and the fluid is flowing through the second flow line.

US Pat. No. 9,786,400

ADJUSTABLE APERTURE DEVICE WITH INTEGRAL APERTURE HOLES

Agilent Technologies, Inc...

1. An adjustable aperture device for an electromagnetic radiation detecting apparatus, the aperture device comprising:
a position adjustment body configured for adjusting a position of a selected aperture hole of a plurality of selectable aperture
holes, wherein electromagnetic radiation is to propagate through the selected aperture hole;

a guide unit configured for guiding the position adjustment body along a predefined guide direction, wherein the guide unit
is configured for guiding the position adjustment body via exactly two contact points defining degrees of freedom according
to which the position adjustment body is allowed to move;

an aperture body defining the plurality of aperture holes and comprising a plurality of engagement sections configured for
selectively receiving the position adjustment body from the guide unit, wherein the position adjustment body is engagable
in a selectable one of the plurality of engagement sections to thereby select the selected aperture hole from the plurality
of selectable aperture holes;

a pre-loading element configured for pre-loading the position adjustment body towards the aperture body; and
a drive unit configured for driving the aperture body to move so that the position adjustment body is engaged in a respective
one of the plurality of engagement sections.

US Pat. No. 9,694,301

TWO-DIMENSIONAL FLUID SEPARATION WITH CONTROLLED PRESSURE

Agilent Technologies, Inc...

1. A sample separation apparatus for separating a liquid sample, the sample separation apparatus comprising:
a first separation unit configured for separating the liquid sample, the first separation unit comprising an inlet, an outlet,
and a first column between the inlet and the outlet, wherein the first column contains a separating material;

a first pump fluidically coupled to the first separation unit, the first pump configured for generating a first fluid flow
for conducting the liquid sample to be separated through the first separation unit, wherein the first pump comprises a movable
piston;

a second separation unit, arranged downstream of the first separation unit, configured for further separating the liquid sample
or fractions thereof after separation by the first separation unit, the second separation unit comprising an inlet, an outlet,
and a second column between the inlet and the outlet, wherein the second column contains a separating material, and the outlet
of the first separation unit is fluidically coupled to the inlet of the second separation unit via a first fluidic path between
the outlet of the first separation unit and the inlet of the second separation unit;

a second pump configured for generating a second fluid flow for conducting at least part of the liquid sample after separation
by the first separation unit, through the second separation unit, wherein the second pump comprises a movable piston, and
the second pump is fluidically coupled to the first fluidic path via a second fluidic path;

a flow coupler comprising at least two fluid inlet terminals in fluid communication with each other and a fluid outlet terminal
in fluid communication with the at least two fluid inlet terminals, the fluid outlet terminal being fluidically connectable
to the second separation unit;

a fluidic valve comprising a plurality of fluidic interfaces respectively fluidically coupled to the first pump via the first
separation unit, to the second pump, and to the inlet of the second separation unit via the at least two fluid inlet terminals,
the fluidic valve being switchable to a plurality of switching states for performing the separation of the liquid sample in
the second separation unit, wherein the plurality of switching states determine which fluidic interfaces of the plurality
of fluidic interfaces are respectively fluidically coupled to the first pump, to the second pump, and to the at least two
fluid inlet terminals, and the fluidic valve defines at least a portion of the first fluidic path and a portion of the second
fluidic path; and

a control device configured for:
switching the fluidic valve to the plurality of switching states; and
adjusting a pressure at a defined position to a defined value by controlling an operating parameter of at least the second
pump, wherein the defined position is in the first fluidic path or the second fluidic path.

US Pat. No. 9,644,199

IMMOBILIZED TRANSPOSASE COMPLEXES FOR DNA FRAGMENTATION AND TAGGING

Agilent Technologies, Inc...

1. A solid substrate-bound transposase complex, wherein the complex comprises:
a transposase component comprising a first transposase and a second transposase;
an oligonucleotide adapter component comprising a first-oligonucleotide adapter and a second oligonucleotide adapter,
wherein each oligonucleotide adapter comprises at least one double stranded portion that contains a recognition sequence for
a transposase of the complex, and

wherein the first oligonucleotide adapter is bound to the first transposase and the second oligonucleotide adapter is bound
to the second transposase;

a linker component comprising a specific binding pair, one of the members of the specific binding pair being bound to the
first oligonucleotide adapter and the other member of the specific binding pair being bound to a solid substrate, wherein
the second oligonucleotide adapter is not bound to a member of any specific binding pair; and

a solid substrate.

US Pat. No. 9,627,190

ENERGY RESOLVED TIME-OF-FLIGHT MASS SPECTROMETRY

Agilent Technologies, Inc...

1. A time-of-flight mass spectrometry (TOF-MS) system, comprising:
an ion source;
a TOF analyzer comprising an ion accelerator, a flight region, and an ion detector comprising a plurality of channels;
an ion dispersion device configured for dispersing ions from the ion source into a plurality of spatially separated ion beams
based on different kinetic energies of the ions, wherein ions of different kinetic energies travel in the flight region in
spatially separated flight paths and respective channels are aligned with the flight paths; and

a computing device configured for:
receiving an ion measurement signal from the ion detector corresponding to detection of an ion;
determining a time of flight and a kinetic energy of the ion based on the ion measurement signal; and
calculating a mass of the ion by applying a mass calibration function based on the time of flight, the kinetic energy, and
one or more instrument-dependent calibration constants.

US Pat. No. 9,576,778

DATA PROCESSING FOR MULTIPLEXED SPECTROMETRY

Agilent Technologies, Inc...

1. A method for acquiring ion measurement data from a sample, the method comprising:
acquiring raw measurement data generated by an ion detector; and
removing noise from the raw measurement data by:
arranging the raw measurement data into an array of data points, the array comprising a plurality of columns and a plurality
of rows;

for each row, counting a total number of positive data points contained in the row, and determining whether the total number
of positive data points is less than a threshold value, wherein:

if the total number of positive data points is less than the threshold value, then setting all data points in the row to zero;
and

if the total number of positive data points is greater than or equal to the threshold value, then retaining all data points
in the row.

US Pat. No. 9,395,109

EFFICIENT CHILLER FOR A SUPERCRITICAL FLUID CHROMATOGRAPHY PUMP

Agilent Technologies, Inc...

1. A pump head chiller assembly for chilling a pump head of a pump, the pump head chiller assembly comprising:
mobile phase tubing comprising a mobile phase inlet, a mobile phase outlet configured to fluidly communicate with the pump
head, and a multi-turn tubing section between the mobile phase inlet and the mobile phase outlet, wherein the mobile phase
tubing is configured to flow a mobile phase through the mobile phase tubing and toward the pump head;

a Peltier element comprising a cool side and an opposing hot side;
a chiller plate arranged on the cool side, wherein:
the mobile phase tubing is interposed between and in thermal contact with the cool side and the chiller plate, such that the
Peltier element and the chiller plate are configured to pre-chill the mobile phase, including a compressible fluid thereof,
in the mobile phase tubing;

the cool side is in thermal contact with the chiller plate such that the Peltier element removes heat from the chiller plate;
and

the chiller plate is configured for mounting in thermal contact with the pump head such that the chiller plate removes heat
from the pump head; and

a first heat exchanger comprising a cooler block arranged on the hot side and configured to remove heat from the Peltier element
by circulating a coolant through the cooler block.

US Pat. No. 9,995,421

VIBRATION DAMPER FOR VACUUM PUMPS

Agilent Technologies, Inc...

1. An apparatus, comprising:a vibration damper comprising an elastic material and being arranged between a vacuum pump and a vacuum chamber, said elastic material bridging a space between adjacent coupling components associated respectively with the vacuum pump and the vacuum chamber, wherein the vibration damper assumes a resting configuration in response to a compression force with a magnitude lower than a predetermined threshold, and assumes a compressed configuration in response to a compression force with a magnitude greater than the predetermined threshold, the resting configuration forming a connection between the vacuum pump and the vacuum chamber, and the compressed configuration forming a vacuum-tight connection between the vacuum pump and the vacuum chamber, and the compressed configuration having at least one gap preventing connection between the vacuum pump and the vacuum chamber except by the elastic material, the elastic material comprising a toroidal seal seated in a semi-toroidal seat of a flange comprising at least one of the adjacent coupling components.

US Pat. No. 9,905,410

TIME-OF-FLIGHT MASS SPECTROMETRY USING MULTI-CHANNEL DETECTORS

Agilent Technologies, Inc...

1. A time-of-flight mass spectrometry (TOF-MS) system, comprising:
an ion source;
a TOF analyzer comprising an ion accelerator, a flight tube, and an ion detector comprising a plurality of channels; and
an electric field or magnetic field ion dispersion device comprising at least one of an electrode for generation of an electric
field or a magnet for generation of a magnetic field, and configured for dispersing ions from the ion source into a plurality
of spatially separated flight paths in the flight tube,

wherein: before arriving at the detector, a drift energy of the ions is modulated according to a repeating modulation sequence
comprising a plurality of iterations,

the drift energy at each iteration is different from the drift energies of the other iterations, and
respective channels of the ion detector are aligned with the flight paths.
US Pat. No. 9,657,339

METHOD FOR AMPLIFICATION OF TARGET NUCLEIC ACID

Agilent Technologies, Inc...

1. A method for amplifying at least one target nucleic acid in a nucleic acid sample, comprising:
(a) fragmenting the nucleic acid sample to create at least one target fragment comprising said target nucleic acid and comprising
two probe-complementary portions, wherein at least one of said two probe-complementary portions is located at an end of the
target fragment;

(b) contacting said fragmented nucleic acid sample with at least one probe which is provided with an immobilization moiety
and optionally is immobilized on a solid phase by means of said moiety, and which comprises two target fragment-complementary
portions which are complementary in sequence to the probe-complementary portions of the target fragment, wherein said portions
of the probe may be adjacent, or separated by an intervening non-target fragment-complementary portion;

(c) rendering the fragmented nucleic acid sample single-stranded, wherein the step may occur before, contemporaneously with
or after step (b);

(d) allowing the probe-complementary portions of the target fragment to hybridize with the target-fragment complementary portions
of the probe;

(e) if the probe provided in step (b) is not immobilized, immobilizing the probe-target fragment hybrid on a solid phase by
means of said immobilization moiety;

(f) separating non-immobilized nucleic acid fragments from the solid phase;
(g) contacting the solid phase with a ligase to ligate, directly or indirectly, ligatable 5? and 3? ends of the target fragment
whereby the target fragment is circularized, wherein where one of said probe-complementary portions of the target fragment
is not located at an end of the target fragment, the ligatable end, other than that at which a probe-complementary portion
is located, is created by the action of a flap endonuclease or an exonuclease; and

(h) amplifying said circularized target fragment.

US Pat. No. 9,429,499

METHODS, DEVICES, AND SYSTEMS FOR CONTROLLING THE RATE OF GAS DEPRESSURIZATION WITHIN A VIAL CONTAINING A GAS SAMPLE

Agilent Technologies, Inc...

1. A method for controlling a rate of gas depressurization within a vial having a head space to be sampled by a head space
sampling device, said head space sampling device having a ventilation pathway connectable in fluid communication with a lower
pressure environment, and a sample loop connectable between and in fluid communication with said head space and said ventilation
pathway, said method comprising:
establishing fluid communication between said sample loop and said head space,
establishing a sample gas pressure within said head space, said sample gas pressure being greater than the pressure in said
lower pressure environment;

connecting said sample loop in fluid communication with said lower pressure environment through said ventilation pathway;
and

venting gas from said head space through said sample loop to said lower pressure environment, thereby allowing sample gas
to flow from said head space to said sample loop, to provide a predetermined rate of gas depressurization within the head
space and sample loop,

wherein the pressure of sample gas in said head space and sample loop is directly controlled by said predetermined rate of
gas depressurization to a predetermined head space pressure setpoint less than the initial sample gas pressure.

US Pat. No. 9,841,337

PRESSURE DETERMINATION FOR HPLC APPLICATIONS

Agilent Technologies, Inc...

1. A pressure determining unit configured for determining a pressure of a fluid, the pressure determining unit comprising:
a body structure comprising a fluidic path configured for conducting the fluid, wherein the body structure has a first surface
in a first dimension and in a second dimension, and a thickness in a third dimension, and

a deformation detector configured for responding to an elongation into the second dimension of the first surface of the body
structure by generating a signal indicative of a value of the pressure of the fluid in the body structure,

wherein the fluidic path of the body structure comprises one or more first channel segments, each first channel segment having
a height into the third dimension being at least twice its width into the second dimension.

US Pat. No. 9,791,107

PACKET-WISE PROPORTIONING FOLLOWED BY IMMEDIATE LONGITUDINAL MIXING

Agilent Technologies, Inc...

1. A fluid supply system adapted for metering two or more fluids in defined proportions and for supplying a resultant mixture,
the fluid supply system comprising:
a plurality of solvent supply lines, each fluidically connected with a fluid source providing a respective fluid;
a pumping unit comprising a reciprocating element adapted for intaking fluid supplied at an inlet of the pumping unit and
for supplying the pressurized fluid at an outlet of the pumping unit, wherein the pumping unit is adapted for taking in fluids
in defined proportions from selected solvent supply lines and for supplying a pressurized mixture of the fluids at its outlet;

a proportioning valve interposed between the solvent supply lines and the inlet of the pumping unit, the proportioning valve
adapted for modulating solvent composition by sequentially coupling selected ones of the solvent supply lines with the inlet
of the pumping unit in the course of the fluid intake phase of the pumping unit;

a longitudinal mixing unit adapted for mixing longitudinally subsequent sections of the fluids so as to modify their succession
in flow direction, wherein the longitudinal mixing unit is located downstream of the proportioning valve and upstream of the
pumping unit.

US Pat. No. 9,651,426

LIGHT SOURCE WITH CONTROLLABLE LINEAR POLARIZATION

Agilent Technologies, Inc...

1. A light source comprising:
a first laser that emits a beam of linearly polarized light that is characterized by a propagation direction;
a first wire-grid polarization filter characterized by a first linear polarization pass direction and a first actuator for
causing said first linear polarization pass direction to rotate relative to said beam of linearly polarized light, said first
wire-grid polarization filter being positioned such that said beam of linearly polarized light passes through said first wire-grid
polarization filter;

a second wire-grid polarization filter characterized by a second linear polarization pass direction and a second actuator
for causing said second linear polarization pass direction to rotate relative to said beam of linearly polarized light, said
second wire-grid polarization filter being positioned such that said beam of linearly polarized light passes through said
second wire-grid polarization filter after passing through said first wire-grid polarization filter; and

a controller for setting said first and second linear polarization pass directions to provide linearly polarized light having
a specified polarization direction, said first linear polarization pass direction being different from said second linear
polarization pass direction for at least one value of said specified polarization value.

US Pat. No. 9,880,099

SCANNER FOR SPATIALLY OFFSET RAMAN SPECTROSCOPY

Agilent Technologies LDA ...

1. Apparatus for carrying out spatially offset Raman spectroscopy on a sample, comprising:
a light source arranged to form a beam of probe light directed along an optical path to an entry region on the sample;
collection optics arranged to receive said probe light from a collection region on the sample following subsurface scattering
of said probe light within the sample;

a spectral analyser arranged to detect Raman scattering spectral features in the probe light received through the collection
optics; and

a rotatable prism disposed in the optical path, the rotatable prism being arranged such that a spatial offset between the
entry region and the collection region is dependent upon the angle of rotation of the prism.

US Pat. No. 9,873,907

METHOD FOR FRAGMENTING GENOMIC DNA USING CAS9

Agilent Technologies, Inc...

1. A method for fragmenting a genome comprising:
(a) combining a sample containing genomic DNA with a plurality of Cas9-gRNA complexes, wherein each of the Cas9-gRNA complexes
comprises a Cas9 protein and a Cas9-associated guide RNA from a set of at least 10 Cas9-associated guide RNAs that are complimentary
to different, pre-defined, sites in the genome, wherein each said different, predefined, site in the genome is followed by
a protospacer adjacent motif;

(b) incubating the reaction mixture so that the Cas9 protein cleaves the genomic DNA to produce at least 5 fragments of the
genomic DNA having a size within 20% of a chosen size, wherein the chosen size is a length in the range of 1 kb to 100 kb;
and

(c) isolating the at least 5 fragments of the genomic DNA from the remainder of the genome.

US Pat. No. 9,863,877

INFRARED SPECTROMETER AND SCANNER UTILIZING ATTENUATED TOTAL REFLECTION

Agilent Technologies, Inc...

1. An attenuated total reflection (ATR) objective comprising:
a first optical element comprising a planar face;
an input port adapted to receive an input collimated light beam characterized by a central ray, said input port being characterized
by a pivot point through which said central ray passes;

a mask that divides said input collimated light beam of light into first and second portions, said mask preventing light in
said first portion from reaching said planar face; and

a second optical element that focuses said second portion on a point on said planar face such that substantially all of said
second portion is reflected from said planar face, said second optical element collecting light reflected from said planar
face and collimating said collected light into an output collimated light beam that leaves said input port, said output collimated
light beam a central ray coincident with said central ray of said input collimated light beam.

US Pat. No. 9,834,774

METHODS AND COMPOSITIONS FOR RAPID SEAMLESS DNA ASSEMBLY

Agilent Technologies, Inc...

1. A DNA assembly method comprising:
(a) obtaining at least two double-stranded DNA fragments, wherein the at least two double-stranded DNA fragments comprise
ends having sequences at opposite ends that can selectively hybridize with one another to form a circular structure comprising
the at least two double-stranded DNA fragments; and

(b) contacting the at least two double-stranded DNA fragments with:
(i) a DNA polymerase;
(ii) a flap endonuclease; and
(iii) a DNA ligase;under reaction conditions that promote hybridization of the at least two DNA fragments and support the activities of components
(i), (ii) and (iii), wherein the ends of the at least two double-stranded DNA fragments are partially extended by the DNA
polymerase to form a 5?-single stranded overhang, the flap endonuclease cleaves the 5?-single stranded overhang forming a
circular structure having gaps, and the DNA ligase seals the gaps to produce a product comprising an assembled circular DNA
comprising at least a portion of each of the at least two double-stranded DNA fragments.

US Pat. No. 9,782,692

PREVENTION OF PHASE SEPARATION UPON PROPORTIONING AND MIXING FLUIDS

Agilent Technologies, Inc...

1. A liquid supply system configured to supply liquids, the liquid supply system comprising:
a plurality of liquid supply lines, each fluidically connected with a liquid source providing a respective liquid;
a pumping unit comprising an inlet and an outlet, the pumping unit configured to intake liquids at the inlet in defined proportions
from selected ones of the liquid supply lines and to supply a pressurized mixture of the liquids at the outlet;

a proportioning valve interposed between the liquid supply lines and the inlet of the pumping unit, the proportioning valve
configured to modulate solvent composition by sequentially coupling the selected ones of the liquid supply lines with the
inlet of the pumping unit while the pumping unit is intaking liquids;

a liquid packet supply unit configured to control the proportioning valve to supply the liquids from the selected ones of
the liquid supply lines as of a sequence of liquid packets, the liquid packets comprising a packet of first liquid and a packet
of second liquid, wherein the first liquid and the second liquid are media capable of forming separate phases upon direct
interaction with each other; and

a phase separation inhibiting unit configured to communicate with the liquid packet supply unit to inhibit the forming of
separate phases by inserting an intermediate liquid packet between the packet of first liquid and the packet of second liquid.

US Pat. No. 9,732,113

DENDRIMERIC DYE-CONTAINING OLIGONUCLEOTIDE PROBES AND METHODS OF PREPARATION AND USES THEREOF

AGILENT TECHNOLOGIES, INC...

1. An oligonucleotide probe, comprising:
(a) a nucleic acid sequence capable of hybridization with genomic DNA or a cellular RNA from a cell of interest;
(b) a primer sequence that is covalently attached to the 5? end of the nucleic acid sequence of (a); and
(c) a dendrimeric group that is covalently attached to the primer sequence of (b) and comprises two or more fluorescent moieties.

US Pat. No. 9,751,085

SINGLE-PIECE FERRULE WITH UNDERCUT TAPERING PART

Agilent Technologies, Inc...

1. A single-piece ferrule for a fitting for coupling a capillary to another component of a fluidic device, the ferrule comprising:
a single-piece ferrule body of unitary construction;
wherein the ferrule body comprises a cone end, a compression end, and a lumen extending from the cone end to the compression
end and configured for receiving at least a part of the capillary;

wherein the ferrule body comprises a tapering front part at the cone end configured for forming a sealed connection with a
housing of the fitting, the tapering front part comprising a front face, a back face, and a lateral surface tapering from
the back face toward the front face;

wherein a back side of the tapering front part has an annular undercut formed as a circumferential recess extending into the
back face along an extension direction of the lumen and tapering inwardly in a direction extending from the compression end
to the cone end; and

wherein the ferrule body has a tapering middle part adjacent to the annular undercut of the tapering front part.

US Pat. No. 10,073,012

DEBRIS FILTER FOR FLUIDIC MEASUREMENT WITH RECESS SIZE DECREASING IN FLUID FLOW DIRECTION

Agilent Technologies, Inc...

1. A filter for filtering debris out of a fluid flowing along a fluid flow direction in a fluidic member of a sample separation device, the filter comprising:a plurality of filter structures, stacked along the fluid flow direction and each filter structure having voids with an exactly defined uniform void size for each filter structure;
wherein a plurality of the voids of each stacked filter structure comprises a substantially uniform diameter extending entirely through a thickness of each stack, with the uniform void size decreasing along the fluid flow direction from stack to stack in order to produce fraction-wise filtering of the debris with larger particles filtered out relatively early in the fluid flow direction through the stacked filter structure.

US Pat. No. 9,850,521

IN VITRO ASSAY BUFFER FOR CAS9

Agilent Technologies, Inc...

1. A reaction mixture for in vitro cleavage of a nucleic acid target comprising:
a) a Cas9 protein;
b) a guide RNA;
c) a salt;
d) a buffering agent;
e) a nucleic acid target; and
f) a non-ionic surfactant, wherein the non-ionic surfactant is a polyoxyethylene or polyoxypropylene surfactant, a glycosidic
surfactant, or a bile salt surfactant;
wherein components a)-f) are combined in vitro.

US Pat. No. 9,823,226

HPLC SAMPLE INTRODUCTION WITH COUPLING SAMPLE RESERVOIRS IN PARALLEL BETWEEN MOBILE PHASE DRIVE AND SEPARATION UNIT

Agilent Technologies, Inc...

1. A sample dispatcher configured for individually introducing a plurality of portions of one or more sample fluids into a
flow of a mobile phase of a separation system configured for separating compounds of the sample fluids, wherein the separation
system comprises a mobile phase drive configured for driving the mobile phase through a separation unit configured for separating
compounds of the sample fluids in the mobile phase, the sample dispatcher comprising:
a plurality of sample reservoirs, each configured for receiving and temporarily storing a respective sample fluid portion
or at least a part thereof;

wherein the sample dispatcher is configured for selectively coupling one of the plurality of sample reservoirs between the
mobile phase drive and the separation unit; and

the sample dispatcher is further configured for coupling at least two of the plurality of sample reservoirs in parallel between
the mobile phase drive and the separation unit.

US Pat. No. 9,709,563

PASSIVATION OF SURFACES AFTER LIGAND COUPLING

Agilent Technologies, Inc...

1. A passivated substrate for use in assays, comprising
at least one covalently bonded ligand having specific binding activity for a molecule, wherein the covalently bonded ligand
is directly bonded to a substrate; and

at least one covalently bonded blocking agent, wherein the blocking agent is a hydrophilic polymer directly bonded to the
substrate; and, further the blocking agent and the ligand have at least one additional covalent bond to one another.

US Pat. No. 9,631,618

DETERMINATION OF A FLUID LOSS OF A PISTON PUMP BASED ON EVACUATION TIME VARIATION

Agilent Technologies, Inc...

1. An arrangement, comprising
a piston pump for delivering fluid, the piston pump comprising a piston chamber and a piston arranged such that it can reciprocate
in the piston chamber;

an additional piston pump in fluid connection with the piston pump, the additional piston pump comprising an additional piston
chamber and an additional piston arranged such that it can reciprocate in the additional piston chamber; and

a device for determining a leakage of fluid in the piston pump, the device comprising:
a control unit for controlling the piston in such a manner that the piston executes two piston chamber evacuation processes
with differing evacuation times, in each case for at least partial evacuating of fluid being located in the piston chamber;
and

a determination unit for determining a leakage of fluid based on a comparison of fluid quantities evacuated from the piston
chamber in the two piston chamber evacuation processes,

wherein:
the additional piston pump is arranged so as to accommodate at least temporarily a proportion of the fluid evacuated during
the piston chamber evacuation processes; and

the determination unit is set up so as to determine the leakage based on a difference of a volume of fluid lost within different
measurement intervals corresponding to the two piston chamber evacuation processes, wherein a residence time of the piston
pump in two different pump states is of different length, and wherein the two different pump states comprise a first pump
state in which the piston chamber is decoupled from the additional piston chamber and a second pump state in which the piston
chamber is coupled to the additional piston chamber.

US Pat. No. 10,184,835

HIGH DYNAMIC RANGE INFRARED IMAGING SPECTROSCOPY

Agilent Technologies, Inc...

1. An apparatus comprising:a variable attenuator adapted to receive a light beam generated by an MIR laser and that generates an attenuated light beam therefrom characterized by an attenuation level;
a stage adapted to carry a specimen to be scanned said stage moving said specimen in a first direction;
an optical assembly that focuses said attenuated light beam to a point on said specimen, said optical assembly comprising an objective lens that focuses said attenuated light beam to said point and a mirror that is fixed with respect to said objective lens and that directs said light beam into said objective lens, said objective lens and said mirror moving together in a second direction that is orthogonal to said first direction;
a light detector that measures an intensity of light leaving said point on said specimen, said light detector being characterized by a detector dynamic range; and
a controller that forms a plurality of line MIR images from said intensity as a function of position on said specimen, each line image comprising a plurality of pixels, each pixel value being indicative of a reflectivity of said specimen at a corresponding point on said specimen, wherein for at least one position of said stage, multiple line MIR images are formed without changing said one position of said stage with a different level of attenuation of said light beam for each of said line MIR images, said controller combining said plurality of line MIR images to generate a combined MIR line image having a dynamic range greater than said detector dynamic range.
US Pat. No. 9,890,417

SIGNAL AMPLIFICATION OF FLUORESCENCE IN SITU HYBRIDIZATION

Agilent Technologies, Inc...

1. A method of detecting a target nucleic acid, the method comprising:
(a) obtaining a labeled nucleic acid probe for a target nucleic acid, wherein the probe is double-stranded and comprises a
capture tag in only one strand of the probe, and wherein the one strand comprising the capture tag is complementary to the
target nucleic acid;

(b) contacting both strands of the probe, in denatured form, with the target nucleic acid under conditions suitable for nucleic
acid hybridization, to produce a duplex comprising the capture tag and a target nucleic acid;

(c) linking the probe in the duplex to a signal amplification conjugate via the capture tag; and
(d) producing an amplified detectable signal from the signal amplification conjugate in the duplex.

US Pat. No. 9,876,330

WAVELENGTH TUNABLE EXTERNAL CAVITY QUANTUM CASCADE LASER UTILIZING AN ANGLE TUNED IMMERSION GRATING AS A WAVELENGTH SELECTIVE FILTER ELEMENT

Agilent Technologies, Inc...

1. A light source comprising:
a gain chip that amplifies light passing therethrough;
an retro reflecting prism characterized by pivot axis within said retro reflecting prism, an input light direction, an output
light direction and a diffraction grating that receives light emitted by said gain chip traveling in said input light direction,
returns a diffracted light beam to said gain chip along said input light direction and generates an output light beam; and

a first actuator that causes said retro reflecting prism to rotate about said pivot axis in response to a control signal being
coupled to said first actuator.

US Pat. No. 9,850,525

CAS9-BASED ISOTHERMAL METHOD OF DETECTION OF SPECIFIC DNA SEQUENCE

Agilent Technologies, Inc...

1. An isothermal method for detecting in a sample a target nucleic acid strand having (i) a first CAS-targeted site having
a first target sequence and a first PAM site which is 3? to the first target sequence, and (ii) a second CAS-targeted site
having a second target sequence and a second PAM site which is 3? to the second target sequence, the first target sequence
being different and upstream from the second target sequence, the method comprising the steps of:
(a) contacting a sample suspected to contain the target nucleic acid strand with:
a first CAS9 mutant having a single-strand nicking activity,
a first guide RNA (gRNA) targeting the first CAS-targeted site,
a strand-displacing nucleic acid polymerase, and
nucleotides,
under conditions allowing
nicking of the target nucleic acid strand by the CAS9 mutant at the first CAS targeted site, and
strand-displacing by the strand-displacing nucleic acid polymerase to create one or more copies of a section of the target
nucleic acid strand, each copy containing the second CAS-targeted site;

(b) contacting said one or more copies with:
a second CAS9 mutant having a single-strand nicking activity,
a second gRNA targeting the second CAS-targeted site,
a strand-displacing nucleic acid polymerase,
nucleotides, and
one or more circular probes, each having a tag region and a CAS region that is complementary to the second CAS-targeted site,
under conditions allowing
hybridizing of said one or more copies to said one or more circular probes to generate one or more annealed copies,
nicking of said one or more annealed copies by the second CAS9 mutant at the second CAS-targeted site, and
strand-displacing by the strand-displacing nucleic acid polymerase to create one or more extension products of said one or
more annealed copies, each product containing a detecting region that is complementary to the tag region and recognizable
by a detecting agent; and

(c) detecting presence of the one or more extension products using the detecting agent, whereby the presence of the one or
more extension products is an indicator of the presence of the target nucleic acid strand in the sample.

US Pat. No. 9,833,754

SAMPLE DILUTION TO SPECIFIABLE DILUTION RATIO

Agilent Technologies, Inc...

1. A dilution apparatus for diluting a fluidic sample, in accordance with a specifiable dilution ratio, the dilution apparatus
comprising:
a dilution fluid supply device configured for supplying a dilution fluid, at a first quantity per time being flow rate A;
a transport fluid supply device configured for supplying a transport fluid, at a second quantity per time being flow rate
B;

a first fluid accommodation unit configured for accommodating a first fluid volume;
a second fluid accommodation unit configured for accommodating a second fluid volume;
a control device configured for controlling flow of the dilution fluid, the transport fluid and the fluidic sample so that:
in a first operation mode, the fluidic sample, being accommodated in the first fluid accommodation unit, is forced to flow
to the second fluid accommodation unit at the flow rate B of the transport fluid while the fluidic sample is diluted by being
mixed with the dilution fluid at a flow rate A;

in a second operation mode, the mixture of the dilution fluid and the fluidic sample, being accommodated in the second fluid
accommodation unit, is forced to flow from the second fluid accommodation unit to the first fluid accommodation unit while
being further diluted by being mixed with further dilution fluid such that the specifiable dilution ratio is based at least
on flowrates of the dilution fluid compared to flowrates of the transport fluid.

US Pat. No. 9,802,975

PROTECTING GROUPS FOR “Z NUCLEOTIDE” AND METHODS THEREOF

Agilent Technologies, Inc...

1. A compound having the structural formula (I):

wherein
R1 is NO2, CN, F or C1-C4 perfluoroalkyl;

A is O or S;
R2 is a H, halogen, O-methyl or OR2?, wherein R2? is a first protecting group;

R3 is a second protecting group or a phosphoramidite group;

R5 is a third protecting group;

R is a protected amino group of the formula N?C(R?)NR?2 or N(R?)COR? or N(COR?)2, wherein each R? is independently selected from H, linear-, branched-, saturated- or unsaturated-alkyl, aryl, alkaryl and
aralkyl; or wherein R is a protected amino group of the formula N?C(R?)NR?2, wherein NR?2 is joined to form a 5-8 membered ring wherein said ring can contain a heteroatom selected from O or S; and

each of X, Y and Z is independently H, halogen, or a C1-C3 alkyl, wherein two or more of X, Y and Z may together form a 5-8 membered saturated, un-saturated or aryl ring.

US Pat. No. 9,671,375

FLUIDIC CHIP WITH DISPLACABLE PATTERNED LAYER FOR DETECTING FLUID PRESSURE

Agilent Technologies, Inc...

1. A fluidic chip device configured for processing a fluid, the fluidic chip device comprising:
a plurality of layers laminated to one another;
wherein at least a part of the layers comprises a patterned section of an alternating sequence of bars and fluidic channels
for conducting the fluid under pressure along a resultant flow direction, the patterned section having a length along the
resultant flow direction and being configured for being displaceable in response to the pressure such that the length of the
patterned section changes in response to the pressure; and

a pressure detector configured for responding to the displacement of the patterned section by generating a detector signal
being indicative of a value of the pressure.

US Pat. No. 9,580,746

SYNTHESIS OF LONG FISH PROBES

Agilent Technologies, Inc...

1. A method comprising:
(a) synthesizing a set of overlapping oligonucleotides that comprises probe sequences that hybridize to unique sequences in
a chromosome;

(b) assembling the overlapping oligonucleotides in a way that produces one or more double stranded polynucleotides that each
comprises multiple probe sequences, wherein the contiguous nucleotide sequence of each of said one or more double stranded
polynucleotides is at least 95% identical to a sequence of said chromosome;

(c) labeling the one or more double stranded polynucleotides to produce one or more labeled probes; and
(d) hybridizing said labeled probes to an intact chromosome that comprises repeat sequences, in situ.

US Pat. No. 9,799,503

TRAVELING-WELL ION GUIDES AND RELATED SYSTEMS AND METHODS

Agilent Technologies, Inc...

1. An ion guide, comprising:
an entrance end;
an exit end at a distance from the entrance end along a guide axis;
a plurality of electrodes surrounding a guide volume and axially spaced from each other along the guide axis from the entrance
end to the exit end; and

RF electronics configured for:
applying an RF drive signal to the electrodes effective for generating an RF field in the guide volume comprising a plurality
of potential wells distributed along the guide axis, wherein the RF field comprises a waveform effective for moving the potential
wells in at least one set of the electrodes in an axial direction toward the exit end; and

setting a speed at which the potential wells move in the axial direction, such that an effective potential of the RF field
is time averaged to approximate a non-zero magnitude of the effective potential on the guide axis.

US Pat. No. 9,783,791

MUTANT REVERSE TRANSCRIPTASE AND METHODS OF USE

Agilent Technologies, Inc...

1. An isolated mutant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase having increased reverse transcriptase activity
comprising the sequence of SEQ ID NO: 19 with the exception of a mutation at at least one of the following amino acid positions:
E69, E302, W313, L435, N454, and M651,
wherein the mutant MMLV reverse transcriptase comprises at least one of the following mutations:
a glutamic acid to lysine mutation at position E69,
a glutamic acid to lysine mutation at position E302,
a glutamic acid to arginine mutation at position E302,
a tryptophan to phenylalanine mutation at position W313,
a leucine to glycine mutation at position L435,
a leucine to methionine mutation at position L435,
an asparagine to lysine mutation at position N454,
an asparagine to arginine mutation at position N454, and
a methionine to leucine mutation at position M651.

US Pat. No. 9,610,576

HYDROLYTICALLY STABLE ION-EXCHANGE STATIONARY PHASES AND USES THEREOF

Agilent Technologies, Inc...

1. An ion-exchange medium, comprising:
a particulate substrate having an average particle size of about 0.5 ?m to about 100 ?m in diameter;
a hydrolytically stable monolayer of a bi- or poly-functional organosilane covalently attached to a surface of the particulate
substrate and comprising a plurality of cation-exchange sites, the bi- or poly-functional organosilane having the structural
formula of (I) and having a molecular weight less than about 20,000,


wherein:
each of R1, R2, R3, R4, R5 and R6 are independently H, a C1-C6 alkyl group, a Si atom of the surface of the particulate substrate, or a Si atom of another bi- or poly-functional organosilane,

each of Q1 and Q2 is independently selected from a linear or branched, acyclic or cyclic, saturated aliphatic moiety, optionally with one or
more carbon atoms substituted by oxygen, nitrogen or a sulfur heteroatom,

L is an aliphatic spacer group, optionally with one or more carbon atoms substituted by oxygen, nitrogen or sulfur heteroatoms,
R7 is H or a C1-C6 alkyl group,

R9 is H or an alkyl group,

l is 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12, and
Y is —(C?O)—(CH2)j—(C?O)—OR10 or —(C?O)—(CH2)j—U—(CH2)j—(CH?O)—OR10,
wherein R10 is H, U is —NH—, oxygen or a sulfur heteroatom and j is an integer from about 1 to about 6.

US Pat. No. 9,512,163

COMPOSITIONS AND METHODS FOR CONJUGATING OLIGONUCLEOTIDES

Agilent Technologies, Inc...

1. An oligonucleotide derivative having the structure of formula (A):
wherein R3 is a first oligonucleotide; R1 is selected from the group consisting of alkyl, cycloalkyl, aryl, heterocyclyl, and heteroaryl; R2 is a selected from a (C1-C12) hydrocarbyl chain and a polyethylene glycol linker attached to a 5? hydroxy group, a 3? hydroxy group, or an exocyclic amino
group on a nucleobase of the first oligonucleotide, wherein (C1-C12) hydrocarbyl chain is optionally substituted with a group selected from —O—R?, —O—CO—R?, —NR?—R?, —NR?—CO—R?, —CO—NR?—R?,
—CO—R?, —CN, halogen, or a combination thereof, wherein R? and R? are independently H or (C1-C6) hydrocarbyl; Z1 is S, or O, and Z2 is NR4 or S, wherein R4 is selected from H, alkyl, aryl, heterocyclyl, or heteroaryl.

US Pat. No. 9,932,566

CIS-BLOCKED GUIDE RNA

AGILENT TECHNOLOGIES, INC...

1. A guide RNA comprising an RNA molecule or a group of RNA molecules, wherein the guide RNA comprises:a guide sequence complementary to a target site,
a protein-binding sequence with tracrRNA activity, and
a blocking sequence,
wherein the guide sequence is linked to the blocking sequence, optionally by a linker, wherein the guide sequence is complementary to a target sequence, and the blocking sequence is complementary to at least a portion of the guide sequence such that the blocking sequence competes with the target site and an off-target site for hybridization to the guide sequence, and wherein:
(i) hybridization of the guide sequence to the target site is thermodynamically favored over competing hybridization of the guide sequence to the blocking sequence, and
(ii) hybridization of the guide sequence to the blocking sequence is thermodynamically, favored over competing hybridization of the guide sequence to the off-target site.

US Pat. No. 9,792,416

PEAK CORRELATION AND CLUSTERING IN FLUIDIC SAMPLE SEPARATION

Agilent Technologies, Inc...

1. A device for analyzing measurement data, the device comprising:
a processor configured to receive the measurement data, the measurement data comprising a plurality of data sets corresponding
to a plurality of respective measurements, wherein:

the plurality of respective measurements are performed by a fluid separation apparatus in a plurality of respective measurement
runs on a plurality of respective fluidic samples;

each data set comprises a plurality of features indicative of different fractions of one of the plurality of respective fluidic
samples;

each feature represents a combination of a value of a first measurement parameter with a value of a second measurement parameter;
and

the first measurement parameter is selected from the group consisting of: a retention time of a chromatography measurement,
a retention volume of a chromatography measurement, and a mass to charge ratio of a coupled chromatography and mass spectroscopy
measurement;

a cluster determining unit configured to determine feature clusters by clustering features from different data sets corresponding
to the same fraction based on at least one decision criterion, and further configured to determine, from the feature clusters,
a suspicious feature for which a rule for clustering has failed;

a spread determining unit configured to determine for at least a part of the feature clusters a spread of the features within
a respective feature cluster; and

a display unit configured to display at least the part of the feature clusters together with a graphical indication of the
corresponding spread, including displaying the suspicious feature, and further configured to display at least the part of
the feature clusters according to a coordinate system comprising a first axis and a second axis, wherein:

the first axis corresponds to the value of the first measurement parameter; and
the second axis corresponds to the number of the respective measurement run.

US Pat. No. 9,739,794

SAMPLE INJECTOR WITH DISCONNECTABLE INJECTION NEEDLE

Agilent Technologies, Inc...

1. A sample injector for injecting a fluid into a fluidic path between a high pressure pump and a separation column, the sample
injector comprising:
a robot arm configured for moving an injection needle, when being connected to the robot arm, between a fluid container containing
the fluid and a seat in fluid communication with the fluidic path;

the injection needle configured for aspirating the fluid from the fluid container, when the injection needle has been moved
to the fluid container, and for injecting aspirated fluid into the fluidic path, when the injection needle is accommodated
in the seat, the injection needle forming a part of the fluidic path when accommodated in the seat;

the seat configured for accommodating the injection needle and providing fluid communication with the fluidic path;
wherein the robot arm is configured for selectively disconnecting the injection needle from the robot arm when the injection
needle is accommodated in the seat, such that the injection needle remains a part of the fluidic path after being disconnected
from the robot arm; and

wherein the robot arm is configured for performing a further task while the injection needle is disconnected from the robot
arm.

US Pat. No. 9,758,773

THERMOSTABLE TYPE-A DNA POLYMERASE MUTANT WITH INCREASED RESISTANCE TO INHIBITORS IN BLOOD

Agilent Technologies, Inc...

1. An isolated, mutant thermostable Type-A DNA polymerase comprising the amino acid sequence of SEP ID NO: 11, wherein the
isolated, mutant thermostable Type-A DNA polymerase possesses (i) DNA polymerase activity and (ii) a higher resistance to
a polymerization activity inhibitor than the wild-type DNA polymerase from which the isolated, mutant thermostable Type-A
DNA polymerase is derived.
US Pat. No. 9,758,817

METHOD FOR IDENTIFYING A NUCLEIC ACID IN A SAMPLE

Agilent Technologies, Inc...

1. A method of sample analysis comprising:
a) contacting a nucleic acid sample with a first primer and a second primer under PCR conditions to produce a double stranded
product, wherein said second primer comprises a first label and is 5? blocked;

b) contacting said double stranded product with an exonuclease to degrade one strand of said double-stranded product to produce
a single-stranded product, wherein said the strand that is degraded is an extension product of the first primer;

c) contacting the single-stranded product with a third primer under primer extension conditions, wherein said third primer
comprises a second label and wherein said contacting results in hybridization of the third primer to the single stranded product
and extension thereof using said single stranded product as a template to produce a partial duplex that comprises a single
stranded portion and a double stranded portion; and

d) detecting the first and second labels of said partial duplex.

US Pat. No. 9,683,958

NANOFLUIDIC DEVICE FOR CHARGE ANALYSIS OF STRAIGHTENED MOLECULES

Agilent Technologies, Inc...

1. A nanofluidic sensing device comprising:
a) a channel comprising a floor and a ceiling, wherein said floor and said ceiling are spaced by less than 1 ?m and the channel
comprises an entrance end and an exit end that define the longitudinal axis of said channel;

b) an array of charge sensors in the floor and/or ceiling of said channel, arranged along the longitudinal axis of said channel;
c) a capture area in the floor and/or ceiling of said channel at the entrance end of said channel, wherein said capture area
comprises surface exposed groups that affix an end of a functionalized nucleic acid molecule to the capture area; and

d) a first electrode and a second electrode, wherein said first and second electrodes are positioned to provide an electrophoretic
force along the longitudinal axis of said channel, thereby straightening any nucleic acid molecule that is attached to said
capture area and placing a region of interest of said nucleic acid molecule in proximity with a plurality of said charge sensors.

US Pat. No. 9,543,137

SAMPLE DROPLET GENERATION FROM SEGMENTED FLUID FLOW AND RELATED DEVICES AND METHODS

Agilent Technologies, Inc...

1. A sample introduction device, comprising:
a segmented sample array source configured for flowing a linear segmented sample array comprising a plurality of first plugs
alternately separated by a plurality of second plugs, wherein the first plugs comprise a first fluid and analytes and the
second plugs comprise a second fluid different from the first fluid; and

a sample droplet generator configured for generating a stream of droplets from the segmented sample array wherein the droplets
comprise the analytes, the sample droplet generator comprising:

a first tube comprising a first tube inlet communicating with the segmented sample array source and a first tube outlet, the
first tube defining a sample path; and

a second tube surrounding the first tube and comprising a second tube outlet, wherein the second tube defines a gas path between
the first tube and the second tube leading to the second tube outlet, and the second tube outlet is positioned such that the
sample path merges into the gas path, and

wherein the first tube and the second tube extend along a longitudinal axis, and the second tube outlet is positioned at a
downstream axial distance from the first tube outlet.

US Pat. No. 10,327,321

CHAMBERS FOR MICROWAVE PLASMA GENERATION

AGILENT TECHNOLOGIES, INC...

1. A microwave chamber for plasma generation comprising:a launch structure at a first end of the microwave chamber, the launch structure defining a source opening that is configured to receive a microwave source for producing microwave energy;
a termination section at a second end of the microwave chamber opposite the first end, the termination section configured to substantially block propagation of the microwave energy from the second end of the chamber:
an internal wall structure for guiding the microwave energy generated by the microwave source from the first end toward the second end of the microwave chamber, the internal wall structure at least partially defining a cavity configured to house an antenna of the microwave source when the source opening receives the microwave source, wherein the internal wall structure comprises:
an impedance matching section intermediate the first end and the second end:
a capacitive loaded section intermediate the impedance matching section and the second end; and
at least one ridge extending at least partially through the impedance matching section and the capacitive loaded section along a longitudinal axis of the chamber, wherein
the capacitive loaded section defines a first opening extending through a first minor wall and a second opening extending through a second wall, the second opening and the first opening being opposite each other to receive a plasma torch in the capacitive loaded section along a second axis when the antenna of the microwave source is positioned in the cavity of the microwave chamber, the second axis extending through the first opening and the second opening.

US Pat. No. 9,863,921

FLUID INTERFACE BETWEEN FLUID LINES OF DIFFERING CROSS-SECTIONAL AREA

Agilent Technologies, Inc...

1. A fluid transfer device for transferring a fluid from a first fluid channel with a first cross-sectional area at an outlet
side into a common second fluid channel with a second cross-sectional area at an inlet side, the second cross-sectional area
being larger than the first cross-sectional area, the fluid transfer device comprising:
a fluid inlet interface at which the fluid is transferable out of the first fluid channel into the fluid transfer device;
an inlet branch fluidically coupled with the fluid inlet interface, and configured to split the fluid from the first fluid
channel into a plurality of inlet branch channels;

a plurality of outlet branches fluidically coupled with the inlet branch channels, wherein each of the outlet branches is
configured to split the fluid from the inlet branch channels into a respective plurality of outlet branch channels; and

a fluid outlet interface comprising a plurality of outlet openings a) fluidically coupled with the outlet branch channels
and b) forming a two-dimensional array of the outlet openings by which the fluid outlet interface is fluidically coupled with
the common second fluid channel in a two-dimensional manner across the second cross-sectional area, the fluid outlet interface
configured to transfer the fluid in the outlet branch channels into the common second fluid channel via the outlet openings,

wherein the inlet branch, the outlet branches, the inlet branch channels and the outlet branch channels are disposed such
that the fluid exits from the fluid outlet interface, distributed in a two-dimensional manner across the second cross-sectional
area.

US Pat. No. 9,839,941

SYSTEM AND METHOD FOR FACILITATING MANUAL SORTING OF OBJECTS

Agilent Technologies, Inc...

1. An apparatus for facilitating manual sorting of objects, said apparatus comprising:
a display surface comprising a surface that is adapted to be selectively illuminated and is adapted for receiving a plurality
of objects that are to be sorted;

an identification reader that reads identification information stored on said objects; and
a controller that causes an area on said display surface corresponding to one of said objects to be illuminated based on said
identification information and indicates a location to which said one of said objects is to be moved.

US Pat. No. 9,739,661

INFRARED IMAGING SYSTEM WITH AUTOMATIC REFERENCING

Agilent Technologies, Inc...

1. An apparatus comprising:
a tunable mid-infrared (MIR) laser that generates a light beam;
a specimen stage adapted to carry a specimen to be scanned, said stage moving said specimen in a first direction;
an optical assembly that focuses said light beam to a point on said specimen, said optical assembly comprises a scanning assembly
having a focusing lens that focuses said light beam to a point on said specimen and a mirror that moves in a second direction
relative to said stage such that said focusing lens maintains a fixed distance between said focusing lens and said specimen
stage, said first direction being different from said second direction;

a first light detector that measures a first intensity of light leaving said point on said specimen;
a controller that forms a MIR image from said first intensity of light; and
a reference stage that is different from said specimen stage positioned such that said mirror moves over said reference stage
in response to a command from said controller, wherein

said focusing lens and said mirror move together in said second direction over said specimen stage and said reference stage.

US Pat. No. 10,240,196

TRANSPOSASE-RANDOM PRIMING DNA SAMPLE PREPARATION

AGILENT TECHNOLOGIES, INC...

1. A method comprising:(a) contacting a sample comprising double stranded DNA fragments having an average length of from 150 bp to 1.5 Kb with a plurality of transposase duplexes each loaded with an adapter to produce adapter-tagged fragments, wherein the adapter comprises a duplex region comprising a transposase recognition sequence and a 5? overhang region comprising a first primer sequence;
(b) performing a primer extension reaction on the adapter tagged fragments using a random primer to produce random primer extension products, wherein the random primer comprises a random 3? sequence and a 5? sequence comprising a second primer sequence;
(c) amplifying the random primer extension products of (b) by polymerase chain reaction (PCR) using a forward primer comprising the first primer sequence at its 3? end and a reverse primer comprising the second primer sequence at its 3? end to produce PCR amplification products.
US Pat. No. 10,017,879

CHEMICAL ARRAYS AND METHODS OF USING THE SAME

AGILENT TECHNOLOGIES, INC...

1. A method comprising:(a) subjecting an array of a plurality of non-nucleic acid polymer features comprising non-nucleic acid polymer first moieties immobilized on a surface of a solid support via a cleavable domain to conditions sufficient to cleave said cleavable domain and generate a solution phase composition of said non-nucleic acid polymer first moieties, wherein said non-nucleic acid features are arranged on the surface of said solid support in addressable regions and wherein said solution phase composition is a mixture comprising two or more of said non-nucleic acid polymer-first moieties; and
(b) contacting said solution phase composition of said non-nucleic acid polymer-first moieties with one or more reactants to produce a mixture of product molecules that are different from said non-nucleic acid polymer first moieties.
US Pat. No. 9,834,814

SPATIAL MOLECULAR BARCODING OF IN SITU NUCLEIC ACIDS

Agilent Technologies, Inc...

1. A method of processing a sample, comprising:
(a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample;
(b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise
oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the location of the oligonucleotide
on the solid support and wherein the array is made by

(i) synthesizing the oligonucleotides on a solid support, and
(ii) cleaving the oligonucleotides from the solid support in the gas phase,
to produce an array of oligonucleotides that are spatially addressed but not attached to the support;
(c) hybridizing the nucleic acid tags, or a copy of the same, with said oligonucleotides to produce duplexes; and
(d) extending the oligonucleotides in said duplexes to produce extension products that each comprises (i) a molecular barcode
and (ii) a copy of a nucleic acid tag or a complement thereof.

US Pat. No. 9,822,780

SCROLL VACUUM PUMP AND METHOD OF MAINTENANCE INCLUDING REPLACING A TIP SEAL OF A SCROLL VACUUM PUMP

Agilent Technologies, Inc...

1. A method of operating and maintaining a scroll vacuum pump, the method comprising:
running the scroll pump while the pump is connected to a system, at an inlet of the scroll pump, to discharge fluid from the
system;

while the scroll pump is discharging fluid from the system, sensing a first pressure proportional to a first fraction of the
compression ratio of the pump and a second pressure proportional to a second fraction of the compression ratio of the pump;

calculating a monitored pressure value based on both the first and second sensed pressures;
comparing the monitored pressure value based on the first and second sensed pressures with a reference pressure value; and
changing a tip seal, interposed between an axial end of a scroll blade of one of stationary and orbiting plate scrolls and
a plate of the other of the stationary plate and orbiting plate scrolls of the scroll pump, at a point in time after the monitored
pressure value and the reference pressure value differ by at least a predetermined amount.

US Pat. No. 9,784,720

QUICK LOCK CONNECTOR FOR CONNECTING A CAPILLARY TO A FLUIDIC CONDUIT OF A FLUIDIC COMPONENT

Agilent Technologies, Inc...

1. A fitting for providing a fluid connection between a capillary and a fluidic conduit of a fluidic component, the fitting
comprising:
a fitting housing comprising a housing end with an opening;
a force applicator configured for applying a fixing force for fixing the capillary within the fitting, the force applicator
comprising a shaft connecting the force applicator to the fitting housing for pivoting the force applicator relative to the
fitting housing, wherein the capillary is disposed through the opening;

a force limitation mechanism configured for limiting the fixing force being applicable by the force applicator to the capillary;
a force splitter configured for splitting the fixing force into an advance force component for advancing the capillary towards
the fluidic component and into a clamping force component for clamping the capillary within the fitting;

a biasing mechanism arranged between the force applicator and the force splitter, and the biasing mechanism disposed between
the housing end and the force splitter, the biasing mechanism configured for biasing the force splitter against the capillary;
and

a coupling piece arranged as a separate member between the shaft and the biasing mechanism.

US Pat. No. 9,702,374

SPIRAL PUMPING STAGE AND VACUUM PUMP INCORPORATING SUCH PUMPING STAGE

Agilent Technologies, Inc...

1. A spiral pumping stage, comprising:
a stator ring comprising a plurality of spiral channels on at least one face thereof, an inner perimeter, an outer perimeter,
and an outer side wall;

a rotor disc arranged opposite to the at least one face and configured for rotating at high speed;
a main inlet at the inner perimeter;
an outlet at the outer perimeter; and
an additional side inlet at the outer side wall, wherein:
the stator ring is divided into a first circular sector extending to the additional side inlet and a second circular sector
extending on a remaining portion of the stator ring; and

the plurality of spiral channels on the at least one face comprises one or more centripetal pumping channels defined by corresponding
spiral ribs and extending in the first circular sector from respective channel inlets of the one or more centripetal pumping
channels at the outer perimeter toward respective channel outlets of the one or more centripetal pumping channels at the inner
perimeter, and one or more centrifugal pumping channels defined by corresponding spiral ribs and extending in the second circular
sector from respective channel inlets of the one or more centrifugal pumping channels at the inner perimeter toward respective
channel outlets of the one or more centrifugal pumping channels at the outer perimeter.

US Pat. No. 9,638,676

GC COLUMN CONNECTION WITH A PLANAR CONNECTION TO MATING DEVICES

Agilent Technologies, Inc...

1. A fitting for fluidically coupling a gas chromatography (GC) column to another structure, the fitting comprising:
a GC column comprising a tubular element;
a first end that receives a ferrule haying the tubular element disposed therein, the tubular element being oriented in a first
direction; and

a second end fluidically connected to the first end and having an opening oriented in a second direction that is different
than the first direction, the second end comprising a substantially planar portion; and

a channel between the first end and the second end, and a coating is disposed over the channel, wherein the coating reduces
interactions of analytes with the channel.